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Protein

Ras-related protein Rab6

Gene

Rab6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Mediates membrane trafficking during egg chamber growth and organization, possibly upstream of exocyst component Sec5. Also during oogenesis, plays a role, together with BicD but independently of Sec5, in the polarization of the oocyte microtubule cytoskeleton, in the localization of oskar mRNA and in the anterodorsal secretion of grk. Required for anterograde opsin transport through the ER-Golgi complex. Plays a role, together with Rich, in regulating CadN transport in photoreceptor cells which is required for the formation of normal synaptic connections between axons from the inner photoreceptor cells in the eye and postsynaptic cells in the brain medulla layer M6. Necessary for proper development of bristle shafts of macrochaete and microchaete on the head, thorax and scutellum. Modulates Notch signaling. As a key regulator of vesicular traffic, plays a critical role in the regulation of actin organization and is required for normal rates of phagocytic uptake during phagocytosis involved in defense against viral and fungal infection.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279GTP
Nucleotide bindingi67 – 715GTP
Nucleotide bindingi125 – 1284GTP
Nucleotide bindingi155 – 1573GTP

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • axon guidance Source: FlyBase
  • compound eye morphogenesis Source: FlyBase
  • defense response to fungus Source: FlyBase
  • exocytosis Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • protein transport Source: InterPro
  • R7 cell development Source: FlyBase
  • Rab protein signal transduction Source: FlyBase
  • regulation of postsynaptic membrane potential Source: FlyBase
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Keywords - Ligandi

Actin-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab6Imported
Alternative name(s):
Protein warthog1 Publication
Gene namesi
Name:Rab6Imported
Synonyms:wrt1 Publication
ORF Names:CG6601
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015797. Rab6.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasmic, membrane-bounded vesicle Source: FlyBase
  • Golgi apparatus Source: FlyBase
  • Golgi membrane Source: UniProtKB-SubCell
  • neuronal cell body Source: FlyBase
  • perikaryon Source: UniProtKB-SubCell
  • synapse Source: FlyBase
  • vesicle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Larval lethal.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261T → N: Does not affect interaction with Rich. 1 Publication
Mutagenesisi62 – 621R → C: Produces short bristles but does not alter the direction of their growth. 1 Publication
Mutagenesisi71 – 711Q → L: Impairs maturation of ninaE and Rh3. Causes accumulation of membranes at the base of the rhabdomeres and retinal degeneration. Abolishes interaction with Rich. Alters direction of bristle growth and produces aberrations in the circumferential ridges. 3 Publications
Mutagenesisi125 – 1251N → I: Does not affect maturation of ninaE or Rh3. Does not cause accumulation of membranes at the base of the rhabdomeres or retinal degeneration. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Ras-related protein Rab6PRO_0000425445Add
BLAST

Proteomic databases

PaxDbiO18334.
PRIDEiO18334.

Expressioni

Tissue specificityi

Expressed in larval eye, wing and leg imaginal disks and in salivary gland. Expressed in the larval optic lobe, showing an enrichment in the neuropil. In the adult brain, expressed in photoreceptors and mushroom body.2 Publications

Gene expression databases

ExpressionAtlasiO18334. differential.
GenevisibleiO18334. DM.

Interactioni

Subunit structurei

Interacts with Rich and Act5C. Interacts with BicD (via C-terminal domain). Interacts (in GTP-bound) with GCC1/CG10703 and cbs.6 Publications

Protein-protein interaction databases

BioGridi60686. 49 interactions.
MINTiMINT-828566.
STRINGi7227.FBpp0079943.

Structurei

Secondary structure

1
208
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 2010Combined sources
Helixi25 – 3410Combined sources
Beta strandi46 – 5611Combined sources
Beta strandi59 – 6810Combined sources
Helixi72 – 776Combined sources
Helixi79 – 835Combined sources
Beta strandi86 – 938Combined sources
Helixi97 – 1015Combined sources
Helixi103 – 11412Combined sources
Beta strandi117 – 1259Combined sources
Helixi127 – 1326Combined sources
Helixi137 – 14711Combined sources
Beta strandi150 – 1556Combined sources
Turni156 – 1594Combined sources
Helixi162 – 17110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y8EX-ray1.39A/B1-177[»]
ProteinModelPortaliO18334.
SMRiO18334. Positions 7-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO18334.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 499Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
InParanoidiO18334.
KOiK07893.
OMAiLSTTKWI.
OrthoDBiEOG77DJ7M.
PhylomeDBiO18334.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O18334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGDFGNPL RKFKLVFLGE QSVGKTSLIT RFMYDSFDNT YQATIGIDFL
60 70 80 90 100
SKTMYLEDRT VRLQLWDTAG QERFRSLIPS YIRDSTVAVV VYDITNTNSF
110 120 130 140 150
HQTSKWIDDV RTERGSDVII MLVGNKTDLS DKRQVSTEEG ERKAKELNVM
160 170 180 190 200
FIETSAKAGY NVKQLFRRVA AALPGMDSTE NKPSEDMQEV VLKDSPNETK

DPEGGCAC
Length:208
Mass (Da):23,480
Last modified:January 1, 1998 - v1
Checksum:i5812E7A7CB16B133
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84314 mRNA. Translation: BAA21707.1.
AE014134 Genomic DNA. Translation: AAF53168.1.
AY060261 mRNA. Translation: AAL25300.1.
RefSeqiNP_001285869.1. NM_001298940.1.
NP_477172.1. NM_057824.5.
UniGeneiDm.4067.

Genome annotation databases

EnsemblMetazoaiFBtr0080361; FBpp0079943; FBgn0015797.
FBtr0346437; FBpp0312096; FBgn0015797.
GeneIDi34636.
KEGGidme:Dmel_CG6601.
UCSCiCG6601-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84314 mRNA. Translation: BAA21707.1.
AE014134 Genomic DNA. Translation: AAF53168.1.
AY060261 mRNA. Translation: AAL25300.1.
RefSeqiNP_001285869.1. NM_001298940.1.
NP_477172.1. NM_057824.5.
UniGeneiDm.4067.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y8EX-ray1.39A/B1-177[»]
ProteinModelPortaliO18334.
SMRiO18334. Positions 7-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60686. 49 interactions.
MINTiMINT-828566.
STRINGi7227.FBpp0079943.

Proteomic databases

PaxDbiO18334.
PRIDEiO18334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080361; FBpp0079943; FBgn0015797.
FBtr0346437; FBpp0312096; FBgn0015797.
GeneIDi34636.
KEGGidme:Dmel_CG6601.
UCSCiCG6601-RA. d. melanogaster.

Organism-specific databases

CTDi34636.
FlyBaseiFBgn0015797. Rab6.

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
InParanoidiO18334.
KOiK07893.
OMAiLSTTKWI.
OrthoDBiEOG77DJ7M.
PhylomeDBiO18334.

Miscellaneous databases

EvolutionaryTraceiO18334.
GenomeRNAii34636.
NextBioi789437.
PROiO18334.

Gene expression databases

ExpressionAtlasiO18334. differential.
GenevisibleiO18334. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rab proteins of Drosophila melanogaster: novel members of the Rab-protein family."
    Satoh A.K., Tokunaga F., Ozaki K.
    FEBS Lett. 404:65-69(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Oregon-RImported.
    Tissue: HeadImported.
  2. "Rab6 regulation of rhodopsin transport in Drosophila."
    Shetty K.M., Kurada P., O'Tousa J.E.
    J. Biol. Chem. 273:20425-20430(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF GLN-71 AND ASN-125.
  3. "The developmental role of warthog, the notch modifier encoding Drab6."
    Purcell K., Artavanis-Tsakonas S.
    J. Cell Biol. 146:731-740(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ARG-62 AND GLN-71.
    Tissue: Embryo1 Publication and Imaginal disk1 Publication.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Head1 Publication.
  7. "Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis."
    Coutelis J.B., Ephrussi A.
    Development 134:1419-1430(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BICD.
  8. "Rab6 and the secretory pathway affect oocyte polarity in Drosophila."
    Januschke J., Nicolas E., Compagnon J., Formstecher E., Goud B., Guichet A.
    Development 134:3419-3425(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BICD.
  9. "Golgi coiled-coil proteins contain multiple binding sites for Rab family G proteins."
    Sinka R., Gillingham A.K., Kondylis V., Munro S.
    J. Cell Biol. 183:607-615(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GCC1/CG10703 AND CBS.
  10. "Identification and functional analysis of antifungal immune response genes in Drosophila."
    Jin L.H., Shim J., Yoon J.S., Kim B., Kim J., Kim-Ha J., Kim Y.J.
    PLoS Pathog. 4:E1000168-E1000168(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: TISSUE SPECIFICITY.
  12. "Rich regulates target specificity of photoreceptor cells and N-cadherin trafficking in the Drosophila visual system via Rab6."
    Tong C., Ohyama T., Tien A.C., Rajan A., Haueter C.M., Bellen H.J.
    Neuron 71:447-459(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RICH, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF THR-26 AND GLN-71.
  13. "Involvement of Rab6 in the regulation of phagocytosis against virus infection in invertebrates."
    Ye T., Tang W., Zhang X.
    J. Proteome Res. 11:4834-4846(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ACT5C.
  14. "Structure of the Drosophila melanogaster Rab6 GTPase at 1.4A resolution."
    Walden M., Jenkins H.T., Edwards T.A.
    Acta Crystallogr. F 67:744-748(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 1-177 IN COMPLEX WITH GTP-ANALOG.

Entry informationi

Entry nameiRAB6_DROME
AccessioniPrimary (citable) accession number: O18334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.