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Protein

Ras-related protein Rab6

Gene

Rab6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Mediates membrane trafficking during egg chamber growth and organization, possibly upstream of exocyst component Sec5. Also during oogenesis, plays a role, together with BicD but independently of Sec5, in the polarization of the oocyte microtubule cytoskeleton, in the localization of oskar mRNA and in the anterodorsal secretion of grk. Required for anterograde opsin transport through the ER-Golgi complex. Plays a role, together with Rich, in regulating CadN transport in photoreceptor cells which is required for the formation of normal synaptic connections between axons from the inner photoreceptor cells in the eye and postsynaptic cells in the brain medulla layer M6. Necessary for proper development of bristle shafts of macrochaete and microchaete on the head, thorax and scutellum. Modulates Notch signaling. As a key regulator of vesicular traffic, plays a critical role in the regulation of actin organization and is required for normal rates of phagocytic uptake during phagocytosis involved in defense against viral and fungal infection.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27GTP9
Nucleotide bindingi67 – 71GTP5
Nucleotide bindingi125 – 128GTP4
Nucleotide bindingi155 – 157GTP3

GO - Molecular functioni

  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • axon guidance Source: FlyBase
  • compound eye morphogenesis Source: FlyBase
  • defense response to fungus Source: FlyBase
  • exocytosis Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • photoreceptor cell maintenance Source: FlyBase
  • phototransduction Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • R7 cell development Source: FlyBase
  • Rab protein signal transduction Source: FlyBase
  • regulation of postsynaptic membrane potential Source: FlyBase
  • retrograde transport, endosome to Golgi Source: GO_Central
  • retrograde vesicle-mediated transport, Golgi to ER Source: GO_Central
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Keywords - Ligandi

Actin-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1912420. Pre-NOTCH Processing in Golgi.
R-DME-6798695. Neutrophil degranulation.
R-DME-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-DME-6811438. Intra-Golgi traffic.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.
R-DME-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab6Imported
Alternative name(s):
Protein warthog1 Publication
Gene namesi
Name:Rab6Imported
Synonyms:wrt1 Publication
ORF Names:CG6601
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015797. Rab6.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasmic, membrane-bounded vesicle Source: FlyBase
  • cytosol Source: GOC
  • Golgi apparatus Source: FlyBase
  • Golgi membrane Source: UniProtKB-SubCell
  • neuronal cell body Source: FlyBase
  • perikaryon Source: UniProtKB-SubCell
  • synapse Source: FlyBase
  • vesicle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Larval lethal.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26T → N: Does not affect interaction with Rich. 1 Publication1
Mutagenesisi62R → C: Produces short bristles but does not alter the direction of their growth. 1 Publication1
Mutagenesisi71Q → L: Impairs maturation of ninaE and Rh3. Causes accumulation of membranes at the base of the rhabdomeres and retinal degeneration. Abolishes interaction with Rich. Alters direction of bristle growth and produces aberrations in the circumferential ridges. 3 Publications1
Mutagenesisi125N → I: Does not affect maturation of ninaE or Rh3. Does not cause accumulation of membranes at the base of the rhabdomeres or retinal degeneration. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004254451 – 208Ras-related protein Rab6Add BLAST208

Proteomic databases

PaxDbiO18334.
PRIDEiO18334.

Expressioni

Tissue specificityi

Expressed in larval eye, wing and leg imaginal disks and in salivary gland. Expressed in the larval optic lobe, showing an enrichment in the neuropil. In the adult brain, expressed in photoreceptors and mushroom body.2 Publications

Gene expression databases

BgeeiFBgn0015797.
ExpressionAtlasiO18334. baseline.
GenevisibleiO18334. DM.

Interactioni

Subunit structurei

Interacts with Rich and Act5C. Interacts with BicD (via C-terminal domain). Interacts (in GTP-bound) with GCC1/CG10703 and cbs.6 Publications

Protein-protein interaction databases

BioGridi60686. 49 interactors.
MINTiMINT-828566.
STRINGi7227.FBpp0079943.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 20Combined sources10
Helixi25 – 34Combined sources10
Beta strandi46 – 56Combined sources11
Beta strandi59 – 68Combined sources10
Helixi72 – 77Combined sources6
Helixi79 – 83Combined sources5
Beta strandi86 – 93Combined sources8
Helixi97 – 101Combined sources5
Helixi103 – 114Combined sources12
Beta strandi117 – 125Combined sources9
Helixi127 – 132Combined sources6
Helixi137 – 147Combined sources11
Beta strandi150 – 155Combined sources6
Turni156 – 159Combined sources4
Helixi162 – 171Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y8EX-ray1.39A/B1-177[»]
ProteinModelPortaliO18334.
SMRiO18334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO18334.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 49Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
InParanoidiO18334.
KOiK07893.
OMAiMIDVRTN.
OrthoDBiEOG091G0ORZ.
PhylomeDBiO18334.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O18334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGDFGNPL RKFKLVFLGE QSVGKTSLIT RFMYDSFDNT YQATIGIDFL
60 70 80 90 100
SKTMYLEDRT VRLQLWDTAG QERFRSLIPS YIRDSTVAVV VYDITNTNSF
110 120 130 140 150
HQTSKWIDDV RTERGSDVII MLVGNKTDLS DKRQVSTEEG ERKAKELNVM
160 170 180 190 200
FIETSAKAGY NVKQLFRRVA AALPGMDSTE NKPSEDMQEV VLKDSPNETK

DPEGGCAC
Length:208
Mass (Da):23,480
Last modified:January 1, 1998 - v1
Checksum:i5812E7A7CB16B133
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84314 mRNA. Translation: BAA21707.1.
AE014134 Genomic DNA. Translation: AAF53168.1.
AY060261 mRNA. Translation: AAL25300.1.
RefSeqiNP_001285869.1. NM_001298940.1.
NP_477172.1. NM_057824.5.
UniGeneiDm.4067.

Genome annotation databases

EnsemblMetazoaiFBtr0080361; FBpp0079943; FBgn0015797.
FBtr0346437; FBpp0312096; FBgn0015797.
GeneIDi34636.
KEGGidme:Dmel_CG6601.
UCSCiCG6601-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84314 mRNA. Translation: BAA21707.1.
AE014134 Genomic DNA. Translation: AAF53168.1.
AY060261 mRNA. Translation: AAL25300.1.
RefSeqiNP_001285869.1. NM_001298940.1.
NP_477172.1. NM_057824.5.
UniGeneiDm.4067.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y8EX-ray1.39A/B1-177[»]
ProteinModelPortaliO18334.
SMRiO18334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60686. 49 interactors.
MINTiMINT-828566.
STRINGi7227.FBpp0079943.

Proteomic databases

PaxDbiO18334.
PRIDEiO18334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080361; FBpp0079943; FBgn0015797.
FBtr0346437; FBpp0312096; FBgn0015797.
GeneIDi34636.
KEGGidme:Dmel_CG6601.
UCSCiCG6601-RA. d. melanogaster.

Organism-specific databases

CTDi34636.
FlyBaseiFBgn0015797. Rab6.

Phylogenomic databases

eggNOGiKOG0094. Eukaryota.
ENOG410XPBI. LUCA.
InParanoidiO18334.
KOiK07893.
OMAiMIDVRTN.
OrthoDBiEOG091G0ORZ.
PhylomeDBiO18334.

Enzyme and pathway databases

ReactomeiR-DME-1912420. Pre-NOTCH Processing in Golgi.
R-DME-6798695. Neutrophil degranulation.
R-DME-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-DME-6811438. Intra-Golgi traffic.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.
R-DME-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

EvolutionaryTraceiO18334.
GenomeRNAii34636.
PROiO18334.

Gene expression databases

BgeeiFBgn0015797.
ExpressionAtlasiO18334. baseline.
GenevisibleiO18334. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB6_DROME
AccessioniPrimary (citable) accession number: O18334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.