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O18330

- MRJP1_APIME

UniProt

O18330 - MRJP1_APIME

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Protein
Major royal jelly protein 1
Gene
MRJP1
Organism
Apis mellifera (Honeybee)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major royal jelly protein 1: induces the differentiation of honeybee larvae into queens through an Egfr-mediated signaling pathway. Promotes body size increase by activating p70 S6 kinase, stimulates ovary development by augmenting the titer of vitellogenin (Vg) and juvenile hormone, and reduces developmental time by increasing the activity of mitogen-activated protein kinase and inducing the 20-hydroxyecdysone protein (20E). Most abundant protein found in the royal jelly which is the food of the queen honey bee larva. The royal jelly determines the development of the young larvae and is responsible for the high reproductive ability of the honeybee queen.3 Publications
Jellein-1: has antibacterial activity against the Gram-positive bacteria S.aureus ATCC 6535, S.saprophyticus and B.subtilis CCT2471, and the Gram-negative bacteria E.coli CCT1371, E.cloacae ATCC 23355, K.pneumoniae ATCC 13883 and P.aeruginosa ATCC 27853, and antifungal activity against C.albicans. Lack cytolytic activity and does not induce rat peritoneal mast cell degranulation.3 Publications
Jellein-2: has antibacterial activity against the Gram-positive bacteria S.aureus ATCC 6535, S.saprophyticus and B.subtilis CCT2471, and the Gram-negative bacteria E.coli CCT1371, E.cloacae ATCC 23355, K.pneumoniae ATCC 13883 and P.aeruginosa ATCC 27853, and antifungal activity against C.albicans. Lack cytolytic activity and does not induce rat peritoneal mast cell degranulation.3 Publications
Jellein-4: lacks antibacterial and antifungal activity. Lacks cytolytic activity and does not induce rat peritoneal mast cell degranulation.3 Publications

GO - Biological processi

  1. caste determination, influence by environmental factors Source: UniProtKB
  2. defense response to Gram-negative bacterium Source: UniProtKB
  3. defense response to Gram-positive bacterium Source: UniProtKB
  4. defense response to fungus Source: UniProtKB
  5. killing of cells of other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Developmental protein, Fungicide

Names & Taxonomyi

Protein namesi
Recommended name:
Major royal jelly protein 1
Short name:
MRJP-1
Alternative name(s):
56-kDa protein 4
Short name:
p56kP-4
Bee-milk protein
Royalactin
Cleaved into the following 3 chains:
Alternative name(s):
Jelleine-I
Alternative name(s):
Jelleine-II
Alternative name(s):
Jelleine-IV
Gene namesi
Name:MRJP1
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
ProteomesiUP000005203: Unplaced

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei7627. Api m Apalbumin 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 Publications
Add
BLAST
Chaini20 – 432413Major royal jelly protein 1
PRO_0000031043Add
BLAST
Peptidei424 – 4329Jellein-21 Publication
PRO_0000224649
Peptidei424 – 4318Jellein-4
PRO_0000224650
Peptidei425 – 4328Jellein-1
PRO_0000224648

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi144 – 1441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi177 – 1771N-linked (GlcNAc...) Reviewed prediction
Modified residuei431 – 4311Histidine amide; atypical1 Publication
Modified residuei432 – 4321Leucine amide; atypical1 Publication

Post-translational modificationi

Glycosylated.1 Publication
Jellein-2 is probably processed to yield jellein-1 and jellein-4.

Keywords - PTMi

Amidation, Glycoprotein

Proteomic databases

PaxDbiO18330.
PRIDEiO18330.

Expressioni

Tissue specificityi

Found in the hypopharyngeal glands of the worker honeybee.2 Publications

Developmental stagei

Produced in the cephalic glands of both the nurse bee and the forager bee. This bee milk protein changes to alpha-glucosidase in accordance with the age-dependent role change of the worker bee.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO18330.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3386.
HOGENOMiHOG000136640.
InParanoidiO18330.
OMAiERHNIRT.
PhylomeDBiO18330.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR017996. Royal_jelly.
[Graphical view]
PANTHERiPTHR10009. PTHR10009. 1 hit.
PfamiPF03022. MRJP. 1 hit.
[Graphical view]
PRINTSiPR01366. ROYALJELLY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18330-1 [UniParc]FASTAAdd to Basket

« Hide

MTRLFMLVCL GIVCQGTTGN ILRGESLNKS LPILHEWKFF DYDFGSDERR    50
QDAILSGEYD YKNNYPSDID QWHDKIFVTM LRYNGVPSSL NVISKKVGDG 100
GPLLQPYPDW SFAKYDDCSG IVSASKLAID KCDRLWVLDS GLVNNTQPMC 150
SPKLLTFDLT TSQLLKQVEI PHDVAVNATT GKGRLSSLAV QSLDCNTNSD 200
TMVYIADEKG EGLIVYHNSD DSFHRLTSNT FDYDPKFTKM TIDGESYTAQ 250
DGISGMALSP MTNNLYYSPV ASTSLYYVNT EQFRTSDYQQ NDIHYEGVQN 300
ILDTQSSAKV VSKSGVLFFG LVGDSALGCW NEHRTLERHN IRTVAQSDET 350
LQMIASMKIK EALPHVPIFD RYINREYILV LSNKMQKMVN NDFNFDDVNF 400
RIMNANVNEL ILNTRCENPD NDRTPFKISI HL 432
Length:432
Mass (Da):48,886
Last modified:January 1, 1998 - v1
Checksum:i9F42BF08D34A1A7B
GO

Mass spectrometryi

Molecular mass is 953.24±0.17 Da from positions 425 - 432. Determined by ESI. Jellein-1.1 Publication
Molecular mass is 1054.30±0.18 Da from positions 424 - 432. Determined by ESI. Jellein-2.1 Publication
Molecular mass is 942.13±0.17 Da from positions 424 - 431. Determined by ESI. Jellein-4.1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281N → L AA sequence 1 Publication
Sequence conflicti30 – 301S → K AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D79207 mRNA. Translation: BAA23639.1.
AF000633 mRNA. Translation: AAC61895.1.
AF388203 Genomic DNA. Translation: AAM73637.1.
RefSeqiNP_001011579.1. NM_001011579.1.
UniGeneiAme.208.

Genome annotation databases

EnsemblMetazoaiGB14888-RA; GB14888-PA; GB14888.
GeneIDi406090.
KEGGiame:406090.

Cross-referencesi

Web resourcesi

Protein Spotlight

A queen's dinner - Issue 130 of August 2011

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D79207 mRNA. Translation: BAA23639.1 .
AF000633 mRNA. Translation: AAC61895.1 .
AF388203 Genomic DNA. Translation: AAM73637.1 .
RefSeqi NP_001011579.1. NM_001011579.1.
UniGenei Ame.208.

3D structure databases

ProteinModelPortali O18330.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 7627. Api m Apalbumin 1.

Proteomic databases

PaxDbi O18330.
PRIDEi O18330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai GB14888-RA ; GB14888-PA ; GB14888 .
GeneIDi 406090.
KEGGi ame:406090.

Organism-specific databases

CTDi 406090.

Phylogenomic databases

eggNOGi COG3386.
HOGENOMi HOG000136640.
InParanoidi O18330.
OMAi ERHNIRT.
PhylomeDBi O18330.

Family and domain databases

Gene3Di 2.120.10.30. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR017996. Royal_jelly.
[Graphical view ]
PANTHERi PTHR10009. PTHR10009. 1 hit.
Pfami PF03022. MRJP. 1 hit.
[Graphical view ]
PRINTSi PR01366. ROYALJELLY.
ProtoNeti Search...

Publicationsi

  1. "Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L."
    Ohashi K., Natori S., Kubo T.
    Eur. J. Biochem. 249:797-802(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-48; 63-71 AND 361-380, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION.
    Tissue: Hypopharyngeal gland.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-30.
    Tissue: Head.
  3. "Honeybee (Apis mellifera L.) mrjp gene family: computational analysis of putative promoters and genomic structure of mrjp1, the gene coding for the most abundant protein of larval food."
    Malecova B., Ramser J., O'Brien J.K., Janitz M., Judova J., Lehrach H., Simuth J.
    Gene 303:165-175(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Storage-dependent degradation of 57-kDa protein in royal jelly: a possible marker for freshness."
    Kamakura M., Fukuda T., Fukushima M., Yonekura M.
    Biosci. Biotechnol. Biochem. 65:277-284(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-34.
  5. "Jelleines: a family of antimicrobial peptides from the royal jelly of honeybees (Apis mellifera)."
    Fontana R., Mendes M.A., de Souza B.M., Konno K., Cesar L.M., Malaspina O., Palma M.S.
    Peptides 25:919-928(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 424-432, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AMIDATION AT HIS-431 AND LEU-432.
  6. "A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum."
    Kamakura M., Sakaki T.
    Protein Expr. Purif. 45:307-314(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Royalactin induces queen differentiation in honeybees."
    Kamakura M.
    Nature 473:478-483(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMRJP1_APIME
AccessioniPrimary (citable) accession number: O18330
Secondary accession number(s): Q548D6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Exhibits a growth factor-like action on primary-cultured rat hepatocytes by stimulating DNA synthesis and protecting cells from apoptosis induced by serum deprivation. Also activates mitogen-activated protein kinase, as well as protein kinase B, a key regulator of cell survival (1 Publication).
Degraded proportionally to the period of storage, and is completely lost during storage at 40 degrees Celsius for 30 days (1 Publication).

Caution

Neither His-431 nor Leu-432 is followed in the nucleotide sequence by the expected Gly residue that would be required to produce amidation.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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