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O18330

- MRJP1_APIME

UniProt

O18330 - MRJP1_APIME

Protein

Major royal jelly protein 1

Gene

MRJP1

Organism
Apis mellifera (Honeybee)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Major royal jelly protein 1: induces the differentiation of honeybee larvae into queens through an Egfr-mediated signaling pathway. Promotes body size increase by activating p70 S6 kinase, stimulates ovary development by augmenting the titer of vitellogenin (Vg) and juvenile hormone, and reduces developmental time by increasing the activity of mitogen-activated protein kinase and inducing the 20-hydroxyecdysone protein (20E). Most abundant protein found in the royal jelly which is the food of the queen honey bee larva. The royal jelly determines the development of the young larvae and is responsible for the high reproductive ability of the honeybee queen.
    Jellein-1: has antibacterial activity against the Gram-positive bacteria S.aureus ATCC 6535, S.saprophyticus and B.subtilis CCT2471, and the Gram-negative bacteria E.coli CCT1371, E.cloacae ATCC 23355, K.pneumoniae ATCC 13883 and P.aeruginosa ATCC 27853, and antifungal activity against C.albicans. Lack cytolytic activity and does not induce rat peritoneal mast cell degranulation.
    Jellein-2: has antibacterial activity against the Gram-positive bacteria S.aureus ATCC 6535, S.saprophyticus and B.subtilis CCT2471, and the Gram-negative bacteria E.coli CCT1371, E.cloacae ATCC 23355, K.pneumoniae ATCC 13883 and P.aeruginosa ATCC 27853, and antifungal activity against C.albicans. Lack cytolytic activity and does not induce rat peritoneal mast cell degranulation.
    Jellein-4: lacks antibacterial and antifungal activity. Lacks cytolytic activity and does not induce rat peritoneal mast cell degranulation.

    GO - Biological processi

    1. caste determination, influence by environmental factors Source: UniProtKB
    2. defense response to fungus Source: UniProtKB
    3. defense response to Gram-negative bacterium Source: UniProtKB
    4. defense response to Gram-positive bacterium Source: UniProtKB
    5. killing of cells of other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Developmental protein, Fungicide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Major royal jelly protein 1
    Short name:
    MRJP-1
    Alternative name(s):
    56-kDa protein 4
    Short name:
    p56kP-4
    Bee-milk protein
    Royalactin
    Cleaved into the following 3 chains:
    Alternative name(s):
    Jelleine-I
    Alternative name(s):
    Jelleine-II
    Alternative name(s):
    Jelleine-IV
    Gene namesi
    Name:MRJP1
    OrganismiApis mellifera (Honeybee)
    Taxonomic identifieri7460 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
    ProteomesiUP000005203: Unplaced

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei7627. Api m Apalbumin 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19192 PublicationsAdd
    BLAST
    Chaini20 – 432413Major royal jelly protein 1PRO_0000031043Add
    BLAST
    Peptidei424 – 4329Jellein-2PRO_0000224649
    Peptidei424 – 4318Jellein-4PRO_0000224650
    Peptidei425 – 4328Jellein-1PRO_0000224648

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Modified residuei431 – 4311Histidine amide; atypical1 Publication
    Modified residuei432 – 4321Leucine amide; atypical1 Publication

    Post-translational modificationi

    Glycosylated.1 Publication
    Jellein-2 is probably processed to yield jellein-1 and jellein-4.

    Keywords - PTMi

    Amidation, Glycoprotein

    Proteomic databases

    PaxDbiO18330.
    PRIDEiO18330.

    Expressioni

    Tissue specificityi

    Found in the hypopharyngeal glands of the worker honeybee.2 Publications

    Developmental stagei

    Produced in the cephalic glands of both the nurse bee and the forager bee. This bee milk protein changes to alpha-glucosidase in accordance with the age-dependent role change of the worker bee.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO18330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the major royal jelly protein family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3386.
    HOGENOMiHOG000136640.
    InParanoidiO18330.
    OMAiERHNIRT.
    PhylomeDBiO18330.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR017996. Royal_jelly.
    [Graphical view]
    PANTHERiPTHR10009. PTHR10009. 1 hit.
    PfamiPF03022. MRJP. 1 hit.
    [Graphical view]
    PRINTSiPR01366. ROYALJELLY.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O18330-1 [UniParc]FASTAAdd to Basket

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    MTRLFMLVCL GIVCQGTTGN ILRGESLNKS LPILHEWKFF DYDFGSDERR    50
    QDAILSGEYD YKNNYPSDID QWHDKIFVTM LRYNGVPSSL NVISKKVGDG 100
    GPLLQPYPDW SFAKYDDCSG IVSASKLAID KCDRLWVLDS GLVNNTQPMC 150
    SPKLLTFDLT TSQLLKQVEI PHDVAVNATT GKGRLSSLAV QSLDCNTNSD 200
    TMVYIADEKG EGLIVYHNSD DSFHRLTSNT FDYDPKFTKM TIDGESYTAQ 250
    DGISGMALSP MTNNLYYSPV ASTSLYYVNT EQFRTSDYQQ NDIHYEGVQN 300
    ILDTQSSAKV VSKSGVLFFG LVGDSALGCW NEHRTLERHN IRTVAQSDET 350
    LQMIASMKIK EALPHVPIFD RYINREYILV LSNKMQKMVN NDFNFDDVNF 400
    RIMNANVNEL ILNTRCENPD NDRTPFKISI HL 432
    Length:432
    Mass (Da):48,886
    Last modified:January 1, 1998 - v1
    Checksum:i9F42BF08D34A1A7B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281N → L AA sequence (PubMed:11302159)Curated
    Sequence conflicti30 – 301S → K AA sequence (PubMed:11302159)Curated

    Mass spectrometryi

    Molecular mass is 953.24±0.17 Da from positions 425 - 432. Determined by ESI. Jellein-1.1 Publication
    Molecular mass is 1054.30±0.18 Da from positions 424 - 432. Determined by ESI. Jellein-2.1 Publication
    Molecular mass is 942.13±0.17 Da from positions 424 - 431. Determined by ESI. Jellein-4.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D79207 mRNA. Translation: BAA23639.1.
    AF000633 mRNA. Translation: AAC61895.1.
    AF388203 Genomic DNA. Translation: AAM73637.1.
    RefSeqiNP_001011579.1. NM_001011579.1.
    UniGeneiAme.208.

    Genome annotation databases

    EnsemblMetazoaiGB14888-RA; GB14888-PA; GB14888.
    GeneIDi406090.
    KEGGiame:406090.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    A queen's dinner - Issue 130 of August 2011

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D79207 mRNA. Translation: BAA23639.1 .
    AF000633 mRNA. Translation: AAC61895.1 .
    AF388203 Genomic DNA. Translation: AAM73637.1 .
    RefSeqi NP_001011579.1. NM_001011579.1.
    UniGenei Ame.208.

    3D structure databases

    ProteinModelPortali O18330.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 7627. Api m Apalbumin 1.

    Proteomic databases

    PaxDbi O18330.
    PRIDEi O18330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai GB14888-RA ; GB14888-PA ; GB14888 .
    GeneIDi 406090.
    KEGGi ame:406090.

    Organism-specific databases

    CTDi 406090.

    Phylogenomic databases

    eggNOGi COG3386.
    HOGENOMi HOG000136640.
    InParanoidi O18330.
    OMAi ERHNIRT.
    PhylomeDBi O18330.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR017996. Royal_jelly.
    [Graphical view ]
    PANTHERi PTHR10009. PTHR10009. 1 hit.
    Pfami PF03022. MRJP. 1 hit.
    [Graphical view ]
    PRINTSi PR01366. ROYALJELLY.
    ProtoNeti Search...

    Publicationsi

    1. "Change in the mode of gene expression of the hypopharyngeal gland cells with an age-dependent role change of the worker honeybee Apis mellifera L."
      Ohashi K., Natori S., Kubo T.
      Eur. J. Biochem. 249:797-802(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-48; 63-71 AND 361-380, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION.
      Tissue: Hypopharyngeal gland.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-30.
      Tissue: Head.
    3. "Honeybee (Apis mellifera L.) mrjp gene family: computational analysis of putative promoters and genomic structure of mrjp1, the gene coding for the most abundant protein of larval food."
      Malecova B., Ramser J., O'Brien J.K., Janitz M., Judova J., Lehrach H., Simuth J.
      Gene 303:165-175(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Storage-dependent degradation of 57-kDa protein in royal jelly: a possible marker for freshness."
      Kamakura M., Fukuda T., Fukushima M., Yonekura M.
      Biosci. Biotechnol. Biochem. 65:277-284(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-34.
    5. "Jelleines: a family of antimicrobial peptides from the royal jelly of honeybees (Apis mellifera)."
      Fontana R., Mendes M.A., de Souza B.M., Konno K., Cesar L.M., Malaspina O., Palma M.S.
      Peptides 25:919-928(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 424-432, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AMIDATION AT HIS-431 AND LEU-432.
    6. "A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum."
      Kamakura M., Sakaki T.
      Protein Expr. Purif. 45:307-314(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    7. "Royalactin induces queen differentiation in honeybees."
      Kamakura M.
      Nature 473:478-483(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMRJP1_APIME
    AccessioniPrimary (citable) accession number: O18330
    Secondary accession number(s): Q548D6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Exhibits a growth factor-like action on primary-cultured rat hepatocytes by stimulating DNA synthesis and protecting cells from apoptosis induced by serum deprivation. Also activates mitogen-activated protein kinase, as well as protein kinase B, a key regulator of cell survival (PubMed:16290177).1 Publication
    Degraded proportionally to the period of storage, and is completely lost during storage at 40 degrees Celsius for 30 days.1 Publication

    Caution

    Neither His-431 nor Leu-432 is followed in the nucleotide sequence by the expected Gly residue that would be required to produce amidation.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3