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Protein

Serine/threonine-protein phosphatase 2A regulatory subunit pptr-1

Gene

pptr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable regulatory subunit of serine/threonine-protein phosphatase let-92 which negatively regulates the insulin receptor signaling cascade composed of daf-2, age-1, akt-1, akt-2 and sgk-1 by promoting the dephosphorylation of akt-1 on 'Thr-350' (PubMed:19249087). Negatively regulates several functions controlled by the insulin pathway including dauer formation, lifespan, fat storage and stress resistance (PubMed:19249087). Plays a role in the asymmetric segregation of the P granule components during embryonic cell divisions but does not play an essential role in specifying germ cell fate (PubMed:21127218). In adults, required to promote germ cell proliferation and differentiation when exposed to thermic stress (PubMed:21127218).2 Publications

GO - Molecular functioni

  • protein kinase binding Source: WormBase
  • protein phosphatase activator activity Source: WormBase

GO - Biological processi

  • dauer larval development Source: WormBase
  • determination of adult lifespan Source: WormBase
  • negative regulation of insulin receptor signaling pathway Source: WormBase
  • P granule assembly Source: WormBase
  • protein dephosphorylation Source: WormBase
  • regulation of lipid storage Source: WormBase
  • response to heat Source: WormBase
  • signal transduction Source: InterPro

Enzyme and pathway databases

ReactomeiR-CEL-195253. Degradation of beta-catenin by the destruction complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A regulatory subunit pptr-1Curated
Alternative name(s):
Serine/threonine-protein phosphatase 2A 56kDa regulatory subunit pptr-1Curated
Serine/threonine-protein phosphatase 2A regulatory subunit B' pptr-1Curated
Gene namesi
Name:pptr-1Imported
ORF Names:W08G11.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiW08G11.4; CE32003; WBGene00012348; pptr-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants have small gonads and the number of progeny is reduced (PubMed:21127218). At higher temperatures, 20 percent of mutants are sterile with no gametes (PubMed:21127218). Mutant embryos display several defects in P granule partitioning during the first cell divisions. Unlike in wild-type, plg-1-positive granules becomes unstable during mitosis resulting in their disassembly and in the loss of asymmetric segregation of plg-1 between somatic and germline blastomeres (PubMed:21127218). plg-1-positive granules reform during interphase but are fewer and smaller (PubMed:21127218). By the 100-cell stage, only small granules remain, and these are not enriched in Z2/Z3 primordial germ cells compared to wild-type. In addition, asymmetric segregation of cey-2 and nos-2 mRNAs, 2 components of P granules, is lost without affecting their degradation in the somatic lineage after cell division (PubMed:21127218). RNAi-mediated knockdown suppresses constitutive dauer formation, and, increases lifespan, fat storage and thermotolerance and reduces daf-16 nuclear localization in a daf-2 (e1370) mutant background (PubMed:19249087).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004377541 – 542Serine/threonine-protein phosphatase 2A regulatory subunit pptr-1CuratedAdd BLAST542

Proteomic databases

EPDiO18178.
PaxDbiO18178.
PeptideAtlasiO18178.
PRIDEiO18178.

Expressioni

Tissue specificityi

Expressed in pharynx, vulva and spermatheca.1 Publication

Gene expression databases

BgeeiWBGene00012348.

Interactioni

Subunit structurei

Part of a complex consisting of a common heterodimeric core enzyme, composed of catalytic subunit let-92 and constant regulatory subunit paa-1, that associates with a variety of regulatory subunits which confer distinct properties to the holoenzyme (Probable). Interacts with akt-1 but not akt-2 (PubMed:19249087). Interacts with sgk-1 (PubMed:19249087).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
akt-1Q179413EBI-2298122,EBI-1770718

GO - Molecular functioni

  • protein kinase binding Source: WormBase

Protein-protein interaction databases

IntActiO18178. 3 interactors.
STRINGi6239.W08G11.4.

Structurei

3D structure databases

ProteinModelPortaliO18178.
SMRiO18178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2085. Eukaryota.
ENOG410XQJW. LUCA.
GeneTreeiENSGT00550000074525.
HOGENOMiHOG000067326.
InParanoidiO18178.
KOiK11584.
OMAiTEQAYPE.
OrthoDBiEOG091G06HU.
PhylomeDBiO18178.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
PANTHERiPTHR10257. PTHR10257. 1 hit.
PfamiView protein in Pfam
PF01603. B56. 1 hit.
PIRSFiPIRSF028043. PP2A_B56. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

O18178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGSGHSLTG APHQIPPPRT QGAATGGQQL SATANQFVDK IDPFHNKRGT
60 70 80 90 100
SRRLRINNSS RYNVDSAQEL VQLALIKDTA ANEQPALVIE KLVQCQHVFD
110 120 130 140 150
FYDPVAQLKC KEIKRAALNE LIDHITSTKG AIVETIYPAV IKMVAKNIFR
160 170 180 190 200
VLPPSENCEF DPEEDEPTLE VSWPHLQLVY ELFLRFLESP DFQASIGKKY
210 220 230 240 250
IDQRFVLKLL DLFDSEDPRE RDFLKTVLHR IYGKFLGLRA FIRKHINNMF
260 270 280 290 300
LRFVYETDSF NGVGELLEIL GSIINGFALP LKQEHKVFLV KVLLPLHKPK
310 320 330 340 350
CLSLYHAQLA YCVVQFIEKD SSLTPQVFEA LLKFWPRTCS SKEVMFLGEV
360 370 380 390 400
EEILDIIEPE QFKKIIDPLF RQLAKCVSSP HFQVAERALY FWNNEYILSL
410 420 430 440 450
IEDTSSLVMP IMFPALYRIS KEHWNQTIVA LVYNVLKTFM EMNGKLFDEL
460 470 480 490 500
TSTYKGERLR EKQREKDRDA FWKKMEALEL NPPAEGKEVT PSLFPEKLTD
510 520 530 540
YLKKDGPNMT PLPVATAGGG DKSPSVVKKS STGSETTTPA KK
Length:542
Mass (Da):61,835
Last modified:November 25, 2008 - v4
Checksum:i4AA532BACB97CA92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284605 Genomic DNA. Translation: CAB07297.4.
RefSeqiNP_507133.4. NM_074732.5.
UniGeneiCel.6704.

Genome annotation databases

EnsemblMetazoaiW08G11.4; W08G11.4; WBGene00012348.
GeneIDi180100.
KEGGicel:CELE_W08G11.4.
UCSCiW08G11.4. c. elegans.

Similar proteinsi

Entry informationi

Entry namei2A51_CAEEL
AccessioniPrimary (citable) accession number: O18178
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: November 25, 2008
Last modified: November 22, 2017
This is version 124 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families