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Protein

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase

Gene

ogt-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei612 – 6121Proton acceptorBy similarity
Binding sitei954 – 9541UDPBy similarity
Binding sitei957 – 9571UDPBy similarity
Binding sitei1040 – 10401UDPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1010 – 10134UDPBy similarity
Nucleotide bindingi1016 – 10194UDPBy similarity
Nucleotide bindingi1034 – 10363UDPBy similarity

GO - Molecular functioni

  • protein N-acetylglucosaminyltransferase activity Source: WormBase
  • protein serine/threonine phosphatase activity Source: WormBase

GO - Biological processi

  • dauer larval development Source: WormBase
  • energy reserve metabolic process Source: WormBase
  • glycogen metabolic process Source: WormBase
  • lipid storage Source: WormBase
  • protein dephosphorylation Source: WormBase
  • protein O-linked glycosylation Source: WormBase
  • reproduction Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase (EC:2.4.1.-)
Alternative name(s):
O-GlcNAc
OGT
Gene namesi
Name:ogt-1
ORF Names:K04G7.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiK04G7.3a; CE25042; WBGene00003858; ogt-1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11511151UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferasePRO_0000191775Add
BLAST

Proteomic databases

EPDiO18158.
PaxDbiO18158.

Expressioni

Gene expression databases

BgeeiWBGene00003858.
ExpressionAtlasiO18158. baseline.

Interactioni

Protein-protein interaction databases

BioGridi41212. 3 interactions.
DIPiDIP-26521N.
MINTiMINT-1051575.
STRINGi6239.K04G7.3a.

Structurei

3D structure databases

ProteinModelPortaliO18158.
SMRiO18158. Positions 130-1141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati125 – 15834TPR 1Add
BLAST
Repeati193 – 22634TPR 2Add
BLAST
Repeati227 – 26034TPR 3Add
BLAST
Repeati261 – 29434TPR 4Add
BLAST
Repeati295 – 32834TPR 5Add
BLAST
Repeati329 – 36234TPR 6Add
BLAST
Repeati363 – 39634TPR 7Add
BLAST
Repeati397 – 43034TPR 8Add
BLAST
Repeati431 – 46434TPR 9Add
BLAST
Repeati465 – 49834TPR 10Add
BLAST
Repeati499 – 53234TPR 11Add
BLAST
Repeati533 – 56634TPR 12Add
BLAST
Repeati567 – 57711TPR 13; truncatedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi591 – 60717Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 10644Gln-richAdd
BLAST

Domaini

The TPR repeat domain mediates recognition of protein substrates.By similarity

Sequence similaritiesi

Contains 13 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG4626. Eukaryota.
COG0457. LUCA.
COG3914. LUCA.
GeneTreeiENSGT00550000074327.
HOGENOMiHOG000003765.
InParanoidiO18158.
KOiK09667.
OMAiKLAYMPN.
OrthoDBiEOG091G024Y.
PhylomeDBiO18158.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 1 hit.
PF00515. TPR_1. 1 hit.
PF13374. TPR_10. 1 hit.
PF13414. TPR_11. 4 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 12 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 12 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O18158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPNYFQSY NKVIGATGEQ LAPGAVPPHP VLAPSIAPGG VAGVSAANMA
60 70 80 90 100
NIMQTPGFAN LVQQAIRTQL ENQAAQQLAV NQQFQLNGAT AVQQQLLLTP
110 120 130 140 150
QQSLAQPIAL APQPTVVLNG VSETLKKVTE LAHRQFQSGN YVEAEKYCNL
160 170 180 190 200
VFQSDPNNLP TLLLLSAINF QTKNLEKSMQ YSMLAIKVNN QCAEAYSNLG
210 220 230 240 250
NYYKEKGQLQ DALENYKLAV KLKPEFIDAY INLAAALVSG GDLEQAVTAY
260 270 280 290 300
FNALQINPDL YCVRSDLGNL LKAMGRLEEA KVCYLKAIET QPQFAVAWSN
310 320 330 340 350
LGCVFNSQGE IWLAIHHFEK AVTLDPNFLD AYINLGNVLK EARIFDRAVS
360 370 380 390 400
AYLRALNLSG NHAVVHGNLA CVYYEQGLID LAIDTYKKAI DLQPHFPDAY
410 420 430 440 450
CNLANALKEK GSVVEAEQMY MKALELCPTH ADSQNNLANI KREQGKIEDA
460 470 480 490 500
TRLYLKALEI YPEFAAAHSN LASILQQQGK LNDAILHYKE AIRIAPTFAD
510 520 530 540 550
AYSNMGNTLK EMGDSSAAIA CYNRAIQINP AFADAHSNLA SIHKDAGNMA
560 570 580 590 600
EAIQSYSTAL KLKPDFPDAY CNLAHCHQII CDWNDYDKRV RKLVQIVEDQ
610 620 630 640 650
LCKKRLPSVH PHHSMLYPLS HAARIAIAAK HASLCFDKVH VQMLGKTPLI
660 670 680 690 700
HADRFSVQNG QRLRIGYVSS DFGNHPTSHL MQSIPGMHDR SRVEVFCYAL
710 720 730 740 750
SVNDGTNFRS KLMNESEHFV DLSQIPCNGK AAEKIAQDGI HILINMNGYT
760 770 780 790 800
KGARNEIFAL RPAPIQVMWL GYPSTSGATF MDYIITDAVT SPLRLANAFT
810 820 830 840 850
EKLAYMPHTF FIGDHAQMLR HLTDKVVVKD KETTERDSCL IMNTANMDPI
860 870 880 890 900
LAKSEIKEQV LDTEVVSGPN KELVRAEMVL PVLEVPTEPI KQMIMTGQMT
910 920 930 940 950
MNVMEDMNVQ NGLGQSQMHH KAATGEEIPN SVLLTSRAQY QLPDDAIVFC
960 970 980 990 1000
NFNQLYKIDP STLDMWIKIL ENVPKSILWL LRFPYQGEEH IRKYCVERGL
1010 1020 1030 1040 1050
DPSRIVFSNV AAKEEHVRRG QLADVCLDTP LCNGHTTGMD ILWTGTPMVT
1060 1070 1080 1090 1100
MPLESLASRV ATSQLYALGV PELVAKTRQE YVSIAVRLGT DADHLANMRA
1110 1120 1130 1140 1150
KVWMARTSST LFDVKQYCHD MEDLLGQMWK RYESGMPIDH ITNNTETPHG

L
Length:1,151
Mass (Da):128,012
Last modified:October 1, 2002 - v2
Checksum:iD1CE2A19401CC44F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291T → A in AAB63465 (PubMed:9083068).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77412 mRNA. Translation: AAB63465.1.
FO080149 Genomic DNA. Translation: CCD61614.1.
PIRiE88499.
RefSeqiNP_001040860.1. NM_001047395.3.
UniGeneiCel.16882.

Genome annotation databases

EnsemblMetazoaiK04G7.3a; K04G7.3a; WBGene00003858.
GeneIDi176000.
KEGGicel:CELE_K04G7.3.
UCSCiK04G7.3a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77412 mRNA. Translation: AAB63465.1.
FO080149 Genomic DNA. Translation: CCD61614.1.
PIRiE88499.
RefSeqiNP_001040860.1. NM_001047395.3.
UniGeneiCel.16882.

3D structure databases

ProteinModelPortaliO18158.
SMRiO18158. Positions 130-1141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41212. 3 interactions.
DIPiDIP-26521N.
MINTiMINT-1051575.
STRINGi6239.K04G7.3a.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

Proteomic databases

EPDiO18158.
PaxDbiO18158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK04G7.3a; K04G7.3a; WBGene00003858.
GeneIDi176000.
KEGGicel:CELE_K04G7.3.
UCSCiK04G7.3a. c. elegans.

Organism-specific databases

CTDi176000.
WormBaseiK04G7.3a; CE25042; WBGene00003858; ogt-1.

Phylogenomic databases

eggNOGiKOG4626. Eukaryota.
COG0457. LUCA.
COG3914. LUCA.
GeneTreeiENSGT00550000074327.
HOGENOMiHOG000003765.
InParanoidiO18158.
KOiK09667.
OMAiKLAYMPN.
OrthoDBiEOG091G024Y.
PhylomeDBiO18158.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiO18158.

Gene expression databases

BgeeiWBGene00003858.
ExpressionAtlasiO18158. baseline.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 1 hit.
PF00515. TPR_1. 1 hit.
PF13374. TPR_10. 1 hit.
PF13414. TPR_11. 4 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 12 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 12 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGT1_CAEEL
AccessioniPrimary (citable) accession number: O18158
Secondary accession number(s): Q21232
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.