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Protein

Ceramide glucosyltransferase 1

Gene

cgt-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide.1 Publication

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.1 Publication

Pathwayi

GO - Molecular functioni

  1. ceramide glucosyltransferase activity Source: GO_Central

GO - Biological processi

  1. glucosylceramide biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiREACT_310934. Glycosphingolipid metabolism.
UniPathwayiUPA00222.

Protein family/group databases

CAZyiGT21. Glycosyltransferase Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferase 1 (EC:2.4.1.80)
Gene namesi
Name:cgt-1
ORF Names:T06C12.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT06C12.10; CE31986; WBGene00011517; cgt-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei70 – 9021HelicalSequence AnalysisAdd
BLAST
Transmembranei354 – 37421HelicalSequence AnalysisAdd
BLAST
Transmembranei403 – 42321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced brood size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Ceramide glucosyltransferase 1PRO_0000421281Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6239.T06C12.10.2.

Structurei

3D structure databases

ProteinModelPortaliO18037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000012898.
HOGENOMiHOG000039663.
InParanoidiO18037.
KOiK00720.
OMAiCLINSHA.
PhylomeDBiO18037.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13506. Glyco_transf_21. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O18037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAANEVVNL FASQATTPSS LDAVTTLETV STPTFIFPEV SDSQILQLMP
60 70 80 90 100
ATLYSGMNWL RDHLDGFSLL ALSGCIFVSV LYLVHIIAFF YSIYRLHHKV
110 120 130 140 150
EPDPTLPGVS VIKPIVGTDK NLYQNLESFF TSQYHSFELL FCFHSEEDEA
160 170 180 190 200
IEVVRSLIKK HPNIEAKILF EGEPVGMNPK VNNMMPAYRA ARYPLVLISD
210 220 230 240 250
SAIFMRPDGI LDMATTMMSH EKMASVTQIP YCKDRQGFHA AFEQIFFGTS
260 270 280 290 300
HARLYLVGNF LGVVCSSGMS SMMKKSALDE CGGMEKFGEY LAEDYFFAKA
310 320 330 340 350
LTSRGCKAAI STHPALQNSA SVTVLSFFNR IGRWIKLRIA MMPHLMVVEP
360 370 380 390 400
LQDCVTSGLI MAFGLNYLGG YSVYKTFGLH LFYWIVMDFS LMTSMQNGKF
410 420 430 440 450
NFTPFLFVFI WLFREFTSPF IFIKAVLAPT IVWRNNKFKL SWGGRIRTSK
460
NSQKVPEAVS LSKGAV
Length:466
Mass (Da):52,416
Last modified:March 1, 2003 - v2
Checksum:iEBC36C0EF42C5831
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81116 Genomic DNA. Translation: CAB03296.2.
PIRiT24561.
RefSeqiNP_506971.2. NM_074570.4.
UniGeneiCel.23835.

Genome annotation databases

EnsemblMetazoaiT06C12.10; T06C12.10; WBGene00011517.
GeneIDi188169.
KEGGicel:CELE_T06C12.10.
UCSCiT06C12.10.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81116 Genomic DNA. Translation: CAB03296.2.
PIRiT24561.
RefSeqiNP_506971.2. NM_074570.4.
UniGeneiCel.23835.

3D structure databases

ProteinModelPortaliO18037.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T06C12.10.2.

Protein family/group databases

CAZyiGT21. Glycosyltransferase Family 21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT06C12.10; T06C12.10; WBGene00011517.
GeneIDi188169.
KEGGicel:CELE_T06C12.10.
UCSCiT06C12.10.1. c. elegans.

Organism-specific databases

CTDi188169.
WormBaseiT06C12.10; CE31986; WBGene00011517; cgt-1.

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000012898.
HOGENOMiHOG000039663.
InParanoidiO18037.
KOiK00720.
OMAiCLINSHA.
PhylomeDBiO18037.

Enzyme and pathway databases

UniPathwayiUPA00222.
ReactomeiREACT_310934. Glycosphingolipid metabolism.

Miscellaneous databases

NextBioi937850.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR025993. Ceramide_glucosylTrfase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF13506. Glyco_transf_21. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division."
    Nomura K.H., Murata D., Hayashi Y., Dejima K., Mizuguchi S., Kage-Nakadai E., Gengyo-Ando K., Mitani S., Hirabayashi Y., Ito M., Nomura K.
    Glycobiology 21:834-848(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCGT1_CAEEL
AccessioniPrimary (citable) accession number: O18037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.