O17850 (ATX3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ataxin-3 homolog
EC=3.4.19.12

Alternative name(s):
Machado-Joseph disease-like protein

Gene names

Name:	atx-3
ORF Names:	F28F8.6

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with key regulators of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription. Acts as a deubiquitinating enzyme By similarity. UniProtKB P54252
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Nucleus By similarity UniProtKB P54252.
Sequence similarities	Contains 1 Josephin domain. Contains 2 UIM (ubiquitin-interacting motif) repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Repeat
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW synaptic transmission Inferred from mutant phenotype PubMed 16805848. Source: WormBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 17234717. Source: WormBase nucleus Inferred from direct assay PubMed 17234717. Source: WormBase
Molecular_function	omega peptidase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay PubMed 17234717. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc-48.2	P54812	3	EBI-320650,EBI-320265

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ataxin-3 homolog		PRO_0000053835
Regions
	Domain	7 – 178	172	Josephin
	Repeat	219 – 236	18	UIM 1
	Repeat	247 – 264	18	UIM 2
Sites
	Active site	20	1	Nucleophile By similarity
	Active site	117	1	Proton acceptor By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O17850 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 31798FD62667FB19	FASTA	317	35,864
	10 20 30 40 50 60 MSKDDPINSI FFEHQEAALC AQHALNMLLQ DALYKWQDLR DLAIQMDKME QQILGNANPT 70 80 90 100 110 120 PGRSENMNES GYFSIQVLEK ALETFSLKLT NIENPAMVDY KNNPLTARAY ICNLREHWFV 130 140 150 160 170 180 LRKFGNQWFE LNSVNRGPKL LSDTYVSMFL HQVSSEGYSI FVVQGVLPRS DADDLISLCP 190 200 210 220 230 240 VVPPKVTPKK EQKLEKVMTK FFNTVGKRLG GGSGAPPDSQ EEKDLAIAFA MSMETKDGSE 250 260 270 280 290 300 VSRSSAEIDE ENLRKAIELS QAPGPSEPAE IPLLTRSRSS TPPGASEPFS NAEQQRRDRQ 310 KFLERFEKKK EERNDEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Structural modeling of ataxin-3 reveals distant homology to adaptins." Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T. Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81071 Genomic DNA. Translation: CAB03016.1.
PIR	T21511.
RefSeq	NP_506873.1. NM_074472.7.
3D structure databases
ProteinModelPortal	O17850.
SMR	O17850. Positions 7-168.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27501N.
IntAct	O17850. 3 interactions.
MINT	MINT-1090524.
STRING	6239.F28F8.6.1.
Protein family/group databases
MEROPS	C86.003.
Proteomic databases
PaxDb	O17850.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F28F8.6.1; F28F8.6.1; F28F8.6. F28F8.6.2; F28F8.6.2; F28F8.6. F28F8.6.3; F28F8.6.3; F28F8.6.
GeneID	180049.
KEGG	cel:CELE_F28F8.6.
UCSC	F28F8.6.1. c. elegans.
Organism-specific databases
CTD	180049.
WormBase	F28F8.6; CE09760; WBGene00006446; atx-3.
Phylogenomic databases
eggNOG	NOG327234.
GeneTree	ENSGT00390000001830.
HOGENOM	HOG000006034.
InParanoid	O17850.
KO	K11863.
OMA	NALKFWG.
Family and domain databases
InterPro	IPR006155. Josephin. IPR003903. Ubiquitin-int_motif. [Graphical view]
Pfam	PF02099. Josephin. 1 hit. PF02809. UIM. 2 hits. [Graphical view]
PRINTS	PR01233. JOSEPHIN.
SMART	SM00726. UIM. 2 hits. [Graphical view]
PROSITE	PS50957. JOSEPHIN. 1 hit. PS50330. UIM. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	907892.

Entry information

Entry name

ATX3_CAEEL

Accession

Primary (citable) accession number: O17850

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project