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Protein

Carboxypeptidase E

Gene

egl-21

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During FMRFamide-like peptide (FaRPs or FLP) and neuropeptide-like protein (NLP) precursor processing, catalyzes the removal of Arg or Lys residues from the C-terminus following the initial endoprotease cleavage (PubMed:17564681, PubMed:12657671). By processing neuropeptides, modulates basal acetylcholine release at the ventral cord neuromuscular junctions (PubMed:12657671, PubMed:22275875). Involved in egg-laying, defecation and locomotion (PubMed:11813735, PubMed:17564681, PubMed:22275875). By processing FLP neuropeptides, regulates the turning step of male mating behavior (PubMed:17611271). Involved in reducing pharyngeal pumping in response to high CO2 levels (PubMed:25101962).6 Publications

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.2 Publications

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Zinc; catalyticBy similarity1
Metal bindingi104Zinc; catalyticBy similarity1
Metal bindingi232Zinc; catalyticBy similarity1
Active sitei329Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: WormBase
  • metallocarboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • acetylcholine secretion, neurotransmission Source: WormBase
  • insulin processing Source: GO_Central
  • larval feeding behavior Source: UniProtKB
  • protein processing Source: WormBase
  • regulation of eating behavior Source: UniProtKB

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM14.033.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E1 Publication (EC:3.4.17.102 Publications)
Short name:
CPE1 Publication
Alternative name(s):
Egg-laying defective protein 211 Publication
Gene namesi
Name:egl-211 PublicationImported
ORF Names:F01D4.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF01D4.4; CE09156; WBGene00001189; egl-21.

Subcellular locationi

  • Cell projectionaxon 1 Publication
  • Perikaryon 1 Publication
  • Cytoplasmic vesiclesecretory vesicle lumen 1 Publication

  • Note: Predominantly localizes to axons in the nerve ring.1 Publication

GO - Cellular componenti

  • axon Source: WormBase
  • cytoplasmic vesicle Source: WormBase
  • extracellular space Source: GO_Central
  • Golgi apparatus Source: GO_Central
  • neuronal cell body Source: WormBase
  • presynapse Source: GOC
  • secretory granule Source: GO_Central
  • synaptic membrane Source: GO_Central

Keywords - Cellular componenti

Cell projection, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_500415741122 – 472Carboxypeptidase ESequence analysisAdd BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi134N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi385N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi428N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO17754.
PaxDbiO17754.
PeptideAtlasiO17754.

Expressioni

Tissue specificityi

Expression is restricted to the nervous system.1 Publication

Gene expression databases

BgeeiWBGene00001189.

Interactioni

Protein-protein interaction databases

DIPiDIP-26452N.
IntActiO17754. 1 interactor.
MINTiMINT-1076769.
STRINGi6239.F01D4.4.1.

Structurei

3D structure databases

ProteinModelPortaliO17754.
SMRiO17754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
InParanoidiO17754.
KOiK01294.
OMAiINLIHNT.
OrthoDBiEOG091G06A9.
PhylomeDBiO17754.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiView protein in InterPro
IPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
PfamiView protein in Pfam
PF00246. Peptidase_M14. 1 hit.
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiView protein in SMART
SM00631. Zn_pept. 1 hit.
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiView protein in PROSITE
PS00132. CARBOXYPEPT_ZN_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O17754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHAMRPVLL VAALLAVTAH AFLGFGSGST HKDDAEWGHY HNQAQLEAKL
60 70 80 90 100
GEINEKCPEI TTLYEIGQSV EGRPLVVIQF STTPGEHIPT KPEVKLIGNM
110 120 130 140 150
HGNEPIGREL LLRFAETLCN GAINNDKEIV QLLNSTSIHI LPSMNPDGFE
160 170 180 190 200
LALGTEPAQR QWLTGRSNIN GVDLNRDFPD LDSIFYELQK IGVPKFDHLL
210 220 230 240 250
SLFEDNVDRQ PETIAVGQWT LSLPFVLSAN FHEGDLVANY PFDAAIDENS
260 270 280 290 300
QKTAYSASPD DGTFRWLAKS YADNHAHMSK NDHAPCDGTS QDAFARQGGI
310 320 330 340 350
TNGAKWYSVA GGMQDFNYLA TNAMEITLEL SCEKMPEGSQ LPRFWEDNQK
360 370 380 390 400
SIFEYVWKSH SGVKGMVVDA MTGEPIKRAV VWIRNGTETV PVKHPVTTWS
410 420 430 440 450
EGDFYRVLPA GKYEIIVAAE GYDIAAKNVT VENKVRDSAL VVNFALSPAA
460 470
DEPSENEQEQ IAELVNEIAR RR
Length:472
Mass (Da):52,279
Last modified:January 1, 1998 - v1
Checksum:iE3B1AFF682075A8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAB02881.1.
PIRiT20454.
RefSeqiNP_501935.1. NM_069534.6.
UniGeneiCel.17657.

Genome annotation databases

EnsemblMetazoaiF01D4.4.1; F01D4.4.1; WBGene00001189.
F01D4.4.2; F01D4.4.2; WBGene00001189.
F01D4.4.3; F01D4.4.3; WBGene00001189.
GeneIDi177940.
KEGGicel:CELE_F01D4.4.
UCSCiF01D4.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAB02881.1.
PIRiT20454.
RefSeqiNP_501935.1. NM_069534.6.
UniGeneiCel.17657.

3D structure databases

ProteinModelPortaliO17754.
SMRiO17754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-26452N.
IntActiO17754. 1 interactor.
MINTiMINT-1076769.
STRINGi6239.F01D4.4.1.

Protein family/group databases

MEROPSiM14.033.

Proteomic databases

EPDiO17754.
PaxDbiO17754.
PeptideAtlasiO17754.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF01D4.4.1; F01D4.4.1; WBGene00001189.
F01D4.4.2; F01D4.4.2; WBGene00001189.
F01D4.4.3; F01D4.4.3; WBGene00001189.
GeneIDi177940.
KEGGicel:CELE_F01D4.4.
UCSCiF01D4.4.1. c. elegans.

Organism-specific databases

CTDi177940.
WormBaseiF01D4.4; CE09156; WBGene00001189; egl-21.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
InParanoidiO17754.
KOiK01294.
OMAiINLIHNT.
OrthoDBiEOG091G06A9.
PhylomeDBiO17754.

Gene expression databases

BgeeiWBGene00001189.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiView protein in InterPro
IPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
PfamiView protein in Pfam
PF00246. Peptidase_M14. 1 hit.
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiView protein in SMART
SM00631. Zn_pept. 1 hit.
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiView protein in PROSITE
PS00132. CARBOXYPEPT_ZN_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCBPE_CAEEL
AccessioniPrimary (citable) accession number: O17754
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
Last sequence update: January 1, 1998
Last modified: March 15, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.