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Protein

Pyruvate carboxylase 1

Gene

pyc-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.By similarity

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.Curated

Cofactori

Protein has several cofactor binding sites:
  • biotinBy similarity
  • Zn2+By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147ATPSequence analysis1
Binding sitei231ATPSequence analysis1
Binding sitei266ATPSequence analysis1
Active sitei323Sequence analysis1
Metal bindingi568Divalent metal cationBy similarity1
Binding sitei640SubstrateBy similarity1
Metal bindingi737Divalent metal cation; via carbamate groupBy similarity1
Metal bindingi767Divalent metal cationBy similarity1
Metal bindingi769Divalent metal cationBy similarity1
Binding sitei904SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGluconeogenesis
LigandATP-binding, Biotin, Metal-binding, Nucleotide-binding, Pyruvate, Zinc

Enzyme and pathway databases

BRENDAi6.4.1.1 1045
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase 1 (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase 1
Short name:
PCB 1
Gene namesi
Name:pyc-1Imported
ORF Names:D2023.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiD2023.2a ; CE09072 ; WBGene00004258 ; pyc-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002399371 – 1175Pyruvate carboxylase 1Add BLAST1175

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei737N6-carboxylysineBy similarity1
Modified residuei1140N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

EPDiO17732
PaxDbiO17732
PeptideAtlasiO17732
PRIDEiO17732

PTM databases

iPTMnetiO17732

Expressioni

Gene expression databases

BgeeiWBGene00004258
ExpressionAtlasiO17732 baseline and differential

Interactioni

Subunit structurei

Interacts with sir-2.2 and sir-2.3.1 Publication

Protein-protein interaction databases

BioGridi44641, 1 interactor
DIPiDIP-25614N
IntActiO17732, 2 interactors
STRINGi6239.D2023.2a.3

Structurei

3D structure databases

ProteinModelPortaliO17732
SMRiO17732
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 481Biotin carboxylationSequence analysisAdd BLAST451
Domaini151 – 348ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini559 – 828Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST270
Domaini1099 – 1174Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni567 – 571Substrate bindingBy similarity5

Phylogenomic databases

eggNOGiENOG410IU5D Eukaryota
COG1038 LUCA
GeneTreeiENSGT00900000141244
HOGENOMiHOG000282801
InParanoidiO17732
KOiK01958
OMAiGQPHGGF
OrthoDBiEOG091G00X2
PhylomeDBiO17732

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

O17732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSRIPPIF ANVVRQTHYR NYANGVIKPR EFNKVMVANR GEIAIRVFRA
60 70 80 90 100
LTELNKTSVA IYAEQDKNSM HRLKADEAYL VGKGLPPVAA YLTIDQIIET
110 120 130 140 150
ALKHNIDAIH PGYGFLSERS DFAAACQNAG IVFIGPSPDV MARMGDKVAA
160 170 180 190 200
RQAAIEAGVQ VVPGTPGPIT TADEAVEFAK QYGTPIILKA AYGGGGRGIR
210 220 230 240 250
RVDKLEEVEE AFRRSYSEAQ AAFGDGSLFV EKFVERPRHI EVQLLGDHHG
260 270 280 290 300
NIVHLYERDC SVQRRHQKVV EIAPAPALPE GVREKILADA LRLARHVGYQ
310 320 330 340 350
NAGTVEFLVD QKGNYYFIEV NARLQVEHTV TEEITGVDLV QAQIRIAEGK
360 370 380 390 400
SLDDLKLSQE TIQTTGSAIQ CRVTTEDPAK GFQPDSGRIE VFRSGEGMGI
410 420 430 440 450
RLDSASAFAG SVISPHYDSL MVKVIASARN HPNAAAKMIR ALKKFRIRGV
460 470 480 490 500
KTNIPFLLNV LRQPSFLDAS VDTYFIDEHP ELFQFKPSQN RAQKLLNYLG
510 520 530 540 550
EVKVNGPTTP LATDLKPAVV SPPIPYIPAG AKPPTGLRDV LVQRGPTEFA
560 570 580 590 600
KEVRSRPGCM ITDTTFRDAH QSLLATRVRT YDMAAISPFV AQSFNGLFSL
610 620 630 640 650
ENWGGATFDV SMRFLHECPW ERLQTLRKLI PNIPFQCLLR GANAMGYSNY
660 670 680 690 700
PDNVIYKFCE LAVKNGMDVF RVFDSLNYLP NLLVGMEAVG KAGGVVEAAI
710 720 730 740 750
AYTGDVTDKS RDKYDLKYYL NLADQLVKAQ AHILSIKDMA GVLKPEAAKL
760 770 780 790 800
LIGALRDKFP DIPIHVHTHD TSGAGVAAML ECAKAGADVV DAAVDSMSGM
810 820 830 840 850
TSQPSMGAIV ASLQGTKHDT GLSLDDISKY SAYWESTRQL YAPFECATTM
860 870 880 890 900
KSGNADVYKH EIPGGQYTNL QFQAFSLGLG PQFDEVKRMY REANLVLGDI
910 920 930 940 950
IKVTPSSKIV GDLAQFMVQN NLTRETLVDR ADDLSFPKSV VDFMQGNVGQ
960 970 980 990 1000
PPYGFPEPLR TKVLRGKPKV DGRPGENAKP VDLDAVKVEL EEKHGRTLSE
1010 1020 1030 1040 1050
EDVMSYSMFP TVFDEFETFR QQYGPVDKLP TRLFLTGLEI AEEVDVEIES
1060 1070 1080 1090 1100
GKTLAIQLLA EGKLNKRGER EVFFDLNGQM RSIFVVDKEA SKEIVTRPRA
1110 1120 1130 1140 1150
LPGVRGHIGA PMPGDVLELK IKEGDKVTKK QPLFVLSAMK MEMVIDSPIA
1160 1170
GTVKAIHAPQ GTKCSAGDLV VEVEP
Length:1,175
Mass (Da):129,285
Last modified:January 1, 1998 - v1
Checksum:iDE3D21194C21E6AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237467 mRNA Translation: AAF60326.1
Z81052 Genomic DNA Translation: CAB02872.1
PIRiT20346
RefSeqiNP_001256376.1, NM_001269447.1
UniGeneiCel.22714

Genome annotation databases

EnsemblMetazoaiD2023.2a.1; D2023.2a.1; WBGene00004258
D2023.2a.2; D2023.2a.2; WBGene00004258
GeneIDi179616
KEGGicel:CELE_D2023.2
UCSCiD2023.2.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiPYC1_CAEEL
AccessioniPrimary (citable) accession number: O17732
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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