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Protein

Pyruvate carboxylase 1

Gene

pyc-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.By similarity

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.Curated

Cofactori

Protein has several cofactor binding sites:
  • biotinBy similarity
  • Zn2+By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471ATPSequence analysis
Binding sitei231 – 2311ATPSequence analysis
Binding sitei266 – 2661ATPSequence analysis
Active sitei323 – 3231Sequence analysis
Metal bindingi568 – 5681Divalent metal cationBy similarity
Binding sitei640 – 6401SubstrateBy similarity
Metal bindingi737 – 7371Divalent metal cation; via carbamate groupBy similarity
Metal bindingi767 – 7671Divalent metal cationBy similarity
Metal bindingi769 – 7691Divalent metal cationBy similarity
Binding sitei904 – 9041SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Biotin, Metal-binding, Nucleotide-binding, Pyruvate, Zinc

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4094-MONOMER.
BRENDAi6.4.1.1. 1045.
ReactomeiR-CEL-196780. Biotin transport and metabolism.
R-CEL-70263. Gluconeogenesis.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase 1 (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase 1
Short name:
PCB 1
Gene namesi
Name:pyc-1Imported
ORF Names:D2023.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiD2023.2a; CE09072; WBGene00004258; pyc-1.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • mitochondrion Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11751175Pyruvate carboxylase 1PRO_0000239937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei737 – 7371N6-carboxylysineBy similarity
Modified residuei1140 – 11401N6-biotinyllysinePROSITE-ProRule annotationBy similarity

Proteomic databases

EPDiO17732.
PaxDbiO17732.

PTM databases

iPTMnetiO17732.

Expressioni

Gene expression databases

ExpressionAtlasiO17732. baseline.

Interactioni

Protein-protein interaction databases

BioGridi44641. 1 interaction.
DIPiDIP-25614N.
IntActiO17732. 2 interactions.
MINTiMINT-1104864.
STRINGi6239.D2023.2a.3.

Structurei

3D structure databases

ProteinModelPortaliO17732.
SMRiO17732. Positions 489-1174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 481451Biotin carboxylationSequence analysisAdd
BLAST
Domaini151 – 348198ATP-graspPROSITE-ProRule annotationAdd
BLAST
Domaini559 – 828270CarboxyltransferaseSequence analysisAdd
BLAST
Domaini1099 – 117476Biotinyl-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni567 – 5715Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Sequence analysis
Contains 1 biotinyl-binding domain.PROSITE-ProRule annotation
Contains 1 carboxyltransferase domain.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IU5D. Eukaryota.
COG1038. LUCA.
GeneTreeiENSGT00550000074986.
HOGENOMiHOG000282801.
InParanoidiO17732.
KOiK01958.
OMAiYAIQSRV.
PhylomeDBiO17732.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view]
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00867. CPSASE_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O17732-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSRIPPIF ANVVRQTHYR NYANGVIKPR EFNKVMVANR GEIAIRVFRA
60 70 80 90 100
LTELNKTSVA IYAEQDKNSM HRLKADEAYL VGKGLPPVAA YLTIDQIIET
110 120 130 140 150
ALKHNIDAIH PGYGFLSERS DFAAACQNAG IVFIGPSPDV MARMGDKVAA
160 170 180 190 200
RQAAIEAGVQ VVPGTPGPIT TADEAVEFAK QYGTPIILKA AYGGGGRGIR
210 220 230 240 250
RVDKLEEVEE AFRRSYSEAQ AAFGDGSLFV EKFVERPRHI EVQLLGDHHG
260 270 280 290 300
NIVHLYERDC SVQRRHQKVV EIAPAPALPE GVREKILADA LRLARHVGYQ
310 320 330 340 350
NAGTVEFLVD QKGNYYFIEV NARLQVEHTV TEEITGVDLV QAQIRIAEGK
360 370 380 390 400
SLDDLKLSQE TIQTTGSAIQ CRVTTEDPAK GFQPDSGRIE VFRSGEGMGI
410 420 430 440 450
RLDSASAFAG SVISPHYDSL MVKVIASARN HPNAAAKMIR ALKKFRIRGV
460 470 480 490 500
KTNIPFLLNV LRQPSFLDAS VDTYFIDEHP ELFQFKPSQN RAQKLLNYLG
510 520 530 540 550
EVKVNGPTTP LATDLKPAVV SPPIPYIPAG AKPPTGLRDV LVQRGPTEFA
560 570 580 590 600
KEVRSRPGCM ITDTTFRDAH QSLLATRVRT YDMAAISPFV AQSFNGLFSL
610 620 630 640 650
ENWGGATFDV SMRFLHECPW ERLQTLRKLI PNIPFQCLLR GANAMGYSNY
660 670 680 690 700
PDNVIYKFCE LAVKNGMDVF RVFDSLNYLP NLLVGMEAVG KAGGVVEAAI
710 720 730 740 750
AYTGDVTDKS RDKYDLKYYL NLADQLVKAQ AHILSIKDMA GVLKPEAAKL
760 770 780 790 800
LIGALRDKFP DIPIHVHTHD TSGAGVAAML ECAKAGADVV DAAVDSMSGM
810 820 830 840 850
TSQPSMGAIV ASLQGTKHDT GLSLDDISKY SAYWESTRQL YAPFECATTM
860 870 880 890 900
KSGNADVYKH EIPGGQYTNL QFQAFSLGLG PQFDEVKRMY REANLVLGDI
910 920 930 940 950
IKVTPSSKIV GDLAQFMVQN NLTRETLVDR ADDLSFPKSV VDFMQGNVGQ
960 970 980 990 1000
PPYGFPEPLR TKVLRGKPKV DGRPGENAKP VDLDAVKVEL EEKHGRTLSE
1010 1020 1030 1040 1050
EDVMSYSMFP TVFDEFETFR QQYGPVDKLP TRLFLTGLEI AEEVDVEIES
1060 1070 1080 1090 1100
GKTLAIQLLA EGKLNKRGER EVFFDLNGQM RSIFVVDKEA SKEIVTRPRA
1110 1120 1130 1140 1150
LPGVRGHIGA PMPGDVLELK IKEGDKVTKK QPLFVLSAMK MEMVIDSPIA
1160 1170
GTVKAIHAPQ GTKCSAGDLV VEVEP
Length:1,175
Mass (Da):129,285
Last modified:January 1, 1998 - v1
Checksum:iDE3D21194C21E6AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237467 mRNA. Translation: AAF60326.1.
Z81052 Genomic DNA. Translation: CAB02872.1.
PIRiT20346.
RefSeqiNP_001256376.1. NM_001269447.1.
UniGeneiCel.22714.

Genome annotation databases

EnsemblMetazoaiD2023.2a.1; D2023.2a.1; WBGene00004258.
D2023.2a.2; D2023.2a.2; WBGene00004258.
GeneIDi179616.
KEGGicel:CELE_D2023.2.
UCSCiD2023.2.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237467 mRNA. Translation: AAF60326.1.
Z81052 Genomic DNA. Translation: CAB02872.1.
PIRiT20346.
RefSeqiNP_001256376.1. NM_001269447.1.
UniGeneiCel.22714.

3D structure databases

ProteinModelPortaliO17732.
SMRiO17732. Positions 489-1174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44641. 1 interaction.
DIPiDIP-25614N.
IntActiO17732. 2 interactions.
MINTiMINT-1104864.
STRINGi6239.D2023.2a.3.

PTM databases

iPTMnetiO17732.

Proteomic databases

EPDiO17732.
PaxDbiO17732.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiD2023.2a.1; D2023.2a.1; WBGene00004258.
D2023.2a.2; D2023.2a.2; WBGene00004258.
GeneIDi179616.
KEGGicel:CELE_D2023.2.
UCSCiD2023.2.1. c. elegans.

Organism-specific databases

CTDi179616.
WormBaseiD2023.2a; CE09072; WBGene00004258; pyc-1.

Phylogenomic databases

eggNOGiENOG410IU5D. Eukaryota.
COG1038. LUCA.
GeneTreeiENSGT00550000074986.
HOGENOMiHOG000282801.
InParanoidiO17732.
KOiK01958.
OMAiYAIQSRV.
PhylomeDBiO17732.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciRETL1328306-WGS:GSTH-4094-MONOMER.
BRENDAi6.4.1.1. 1045.
ReactomeiR-CEL-196780. Biotin transport and metabolism.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

NextBioi906166.
PROiO17732.

Gene expression databases

ExpressionAtlasiO17732. baseline.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.20.20.70. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005481. BC-like_N.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR003379. Carboxylase_cons_dom.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR009057. Homeodomain-like.
IPR016185. PreATP-grasp_dom.
IPR000891. PYR_CT.
IPR005930. Pyruv_COase.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00289. Biotin_carb_N. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
PF00682. HMGL-like. 1 hit.
PF02436. PYC_OADA. 1 hit.
[Graphical view]
PIRSFiPIRSF001594. Pyruv_carbox. 1 hit.
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01235. pyruv_carbox. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00867. CPSASE_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cadmium-inducible isoform of pyruvate carboxylase from Caenorhabditis elegans."
    Liao V.H.-C., Freedman J.H.
    DNA Seq. 12:137-145(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. Bienvenut W.V.
    Submitted (MAR-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 286-292; 452-462; 495-503; 718-728; 750-756; 892-902; 1021-1032 AND 1053-1063, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPYC1_CAEEL
AccessioniPrimary (citable) accession number: O17732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.