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Protein

Probable S-adenosylmethionine synthase 1

Gene

sams-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Probable S-adenosylmethionine synthase 1 (sams-1), Probable S-adenosylmethionine synthase 3 (sams-3), Probable S-adenosylmethionine synthase 4 (sams-4), Probable S-adenosylmethionine synthase 5 (sams-5)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumBy similarity1
Binding sitei16ATPBy similarity1
Metal bindingi44PotassiumBy similarity1
Binding sitei57MethionineBy similarity1
Binding sitei100MethionineBy similarity1
Binding sitei245ATPBy similarity1
Binding sitei245Methionine; shared with neighboring subunitBy similarity1
Binding sitei268ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei272ATP; shared with neighboring subunitBy similarity1
Binding sitei276ATP; shared with neighboring subunitBy similarity1
Binding sitei276MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168ATPBy similarity3
Nucleotide bindingi234 – 237ATPBy similarity4
Nucleotide bindingi251 – 252ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • negative regulation of lipid storage Source: WormBase
  • one-carbon metabolic process Source: UniProtKB-KW
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-adenosylmethionine synthase 1 (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase 1
Alternative name(s):
Methionine adenosyltransferase 1
Short name:
MAT 1
Gene namesi
Name:sams-1
ORF Names:C49F5.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC49F5.1; CE08852; WBGene00008205; sams-1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001744441 – 403Probable S-adenosylmethionine synthase 1Add BLAST403

Proteomic databases

EPDiO17680.
PaxDbiO17680.
PeptideAtlasiO17680.
PRIDEiO17680.

2D gel databases

World-2DPAGE0011:O17680.
0020:O17680.

Expressioni

Gene expression databases

BgeeiWBGene00008205.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
sams-3P503054EBI-2412749,EBI-2412743
sams-4P503064EBI-2412749,EBI-2412759

Protein-protein interaction databases

IntActiO17680. 4 interactors.
MINTiMINT-3386375.
STRINGi6239.C49F5.1.2.

Structurei

3D structure databases

ProteinModelPortaliO17680.
SMRiO17680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiO17680.
KOiK00789.
OMAiCETQIAY.
OrthoDBiEOG091G08Z2.
PhylomeDBiO17680.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O17680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKFLFTSE SVSEGHPDKM CDQISDAVLD AHLKQDPNAK VACETVTKTG
60 70 80 90 100
MVMLCGEITS KAVVDYQVLV RRVIEKIGFT DSSIGFDHKT CNVLVALEQQ
110 120 130 140 150
SPEIAAGVHV NKDGEDVGAG DQGIMFGYAT DETEETMPLT LILSHKINAE
160 170 180 190 200
LHKLRRNGTL PWVRPDSKSQ VTIEYESRHG ATIPLRVHTV VVSTQHSPDV
210 220 230 240 250
TLEKLRETIL ADVIKKVIPA SLLDESTVFH INPCGTFIVG GPMGDAGVTG
260 270 280 290 300
RKIIVDTYGG WGAHGGGAFS GKDPTKVDRS AAYAARWVAT SLVKAGLAKR
310 320 330 340 350
VLVQLSYAIG IAKPISVLVY AFGTSPLTDE ELHQIVEDSF DLTPGKIIKE
360 370 380 390 400
LDLKRPIYEK TAENGHFGHS EFPWEQPKAL KISPALLEKA KGNPIPATSA

IAH
Length:403
Mass (Da):43,582
Last modified:January 1, 1998 - v1
Checksum:i81A54B625BC7D96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81485 Genomic DNA. Translation: CAB03975.1.
PIRiT20070.
RefSeqiNP_510002.1. NM_077601.3.
UniGeneiCel.5591.

Genome annotation databases

EnsemblMetazoaiC49F5.1.1; C49F5.1.1; WBGene00008205.
C49F5.1.2; C49F5.1.2; WBGene00008205.
C49F5.1.3; C49F5.1.3; WBGene00008205.
GeneIDi181370.
KEGGicel:CELE_C49F5.1.
UCSCiC49F5.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81485 Genomic DNA. Translation: CAB03975.1.
PIRiT20070.
RefSeqiNP_510002.1. NM_077601.3.
UniGeneiCel.5591.

3D structure databases

ProteinModelPortaliO17680.
SMRiO17680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO17680. 4 interactors.
MINTiMINT-3386375.
STRINGi6239.C49F5.1.2.

2D gel databases

World-2DPAGE0011:O17680.
0020:O17680.

Proteomic databases

EPDiO17680.
PaxDbiO17680.
PeptideAtlasiO17680.
PRIDEiO17680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC49F5.1.1; C49F5.1.1; WBGene00008205.
C49F5.1.2; C49F5.1.2; WBGene00008205.
C49F5.1.3; C49F5.1.3; WBGene00008205.
GeneIDi181370.
KEGGicel:CELE_C49F5.1.
UCSCiC49F5.1.1. c. elegans.

Organism-specific databases

CTDi181370.
WormBaseiC49F5.1; CE08852; WBGene00008205; sams-1.

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
GeneTreeiENSGT00500000044811.
HOGENOMiHOG000245710.
InParanoidiO17680.
KOiK00789.
OMAiCETQIAY.
OrthoDBiEOG091G08Z2.
PhylomeDBiO17680.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
ReactomeiR-CEL-156581. Methylation.
R-CEL-1614635. Sulfur amino acid metabolism.
R-CEL-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

PROiO17680.

Gene expression databases

BgeeiWBGene00008205.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETK1_CAEEL
AccessioniPrimary (citable) accession number: O17680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.