O17680 (METK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable S-adenosylmethionine synthase 1
Short name=AdoMet synthase 1
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 1
Short name=MAT 1

Gene names

Name:	sams-1
ORF Names:	C49F5.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro determination of adult lifespan Inferred from mutant phenotype. Source: WormBase lipid metabolic process Inferred from mutant phenotype. Source: WormBase lipid storage Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase negative regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of locomotion Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase translation Inferred from mutant phenotype. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
sams-3	P50305	4	EBI-2412749,EBI-2412743
sams-4	P50306	4	EBI-2412749,EBI-2412759

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Probable S-adenosylmethionine synthase 1		PRO_0000174444
Regions
	Nucleotide binding	118 – 123	6	ATP Potential
Sites
	Metal binding	18	1	Magnesium By similarity
	Metal binding	44	1	Potassium By similarity
	Metal binding	270	1	Potassium By similarity
	Metal binding	278	1	Magnesium By similarity
	Binding site	146	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O17680 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 81A54B625BC7D96B	FASTA	403	43,582
	10 20 30 40 50 60 MSSKFLFTSE SVSEGHPDKM CDQISDAVLD AHLKQDPNAK VACETVTKTG MVMLCGEITS 70 80 90 100 110 120 KAVVDYQVLV RRVIEKIGFT DSSIGFDHKT CNVLVALEQQ SPEIAAGVHV NKDGEDVGAG 130 140 150 160 170 180 DQGIMFGYAT DETEETMPLT LILSHKINAE LHKLRRNGTL PWVRPDSKSQ VTIEYESRHG 190 200 210 220 230 240 ATIPLRVHTV VVSTQHSPDV TLEKLRETIL ADVIKKVIPA SLLDESTVFH INPCGTFIVG 250 260 270 280 290 300 GPMGDAGVTG RKIIVDTYGG WGAHGGGAFS GKDPTKVDRS AAYAARWVAT SLVKAGLAKR 310 320 330 340 350 360 VLVQLSYAIG IAKPISVLVY AFGTSPLTDE ELHQIVEDSF DLTPGKIIKE LDLKRPIYEK 370 380 390 400 TAENGHFGHS EFPWEQPKAL KISPALLEKA KGNPIPATSA IAH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81485 Genomic DNA. Translation: CAB03975.1.
PIR	T20070.
RefSeq	NP_510002.1. NM_077601.2.
UniGene	Cel.5591.
3D structure databases
ProteinModelPortal	O17680.
SMR	O17680. Positions 5-380.
ModBase	Search...
Protein-protein interaction databases
IntAct	O17680. 5 interactions.
MINT	MINT-3386375.
STRING	O17680.
2D gel databases
World-2DPAGE	0011:O17680. 0020:O17680.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C49F5.1.1; C49F5.1.1; C49F5.1. C49F5.1.2; C49F5.1.2; C49F5.1. C49F5.1.3; C49F5.1.3; C49F5.1.
GeneID	181370.
KEGG	cel:C49F5.1.
UCSC	C49F5.1.1. c. elegans.
Organism-specific databases
CTD	181370.
WormBase	C49F5.1; CE08852; WBGene00008205; sams-1.
Phylogenomic databases
eggNOG	meNOG06160.
GeneTree	EMGT00050000000100.
HOGENOM	HBG443662.
InParanoid	O17680.
OMA	VMLCGEI.
PhylomeDB	O17680.
Gene expression databases
ArrayExpress	O17680.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
KO	K00789.
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	913642.

Entry information

Entry name

METK1_CAEEL

Accession

Primary (citable) accession number: O17680

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project