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Protein

Probable S-adenosylmethionine synthase 1

Gene

sams-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Probable S-adenosylmethionine synthase 1 (sams-1), Probable S-adenosylmethionine synthase 3 (sams-3), Probable S-adenosylmethionine synthase 4 (sams-4), Probable S-adenosylmethionine synthase 5 (sams-5)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumBy similarity1
Binding sitei16ATPBy similarity1
Metal bindingi44PotassiumBy similarity1
Binding sitei57MethionineBy similarity1
Binding sitei100MethionineBy similarity1
Binding sitei245ATPBy similarity1
Binding sitei245Methionine; shared with neighboring subunitBy similarity1
Binding sitei268ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei272ATP; shared with neighboring subunitBy similarity1
Binding sitei276ATP; shared with neighboring subunitBy similarity1
Binding sitei276MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168ATPBy similarity3
Nucleotide bindingi234 – 237ATPBy similarity4
Nucleotide bindingi251 – 252ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • negative regulation of lipid storage Source: WormBase
  • one-carbon metabolic process Source: UniProtKB-KW
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-CEL-156581 Methylation
R-CEL-1614635 Sulfur amino acid metabolism
R-CEL-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-adenosylmethionine synthase 1 (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase 1
Alternative name(s):
Methionine adenosyltransferase 1
Short name:
MAT 1
Gene namesi
Name:sams-1
ORF Names:C49F5.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi

Organism-specific databases

WormBaseiC49F5.1 ; CE08852 ; WBGene00008205 ; sams-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001744441 – 403Probable S-adenosylmethionine synthase 1Add BLAST403

Proteomic databases

EPDiO17680
PaxDbiO17680
PeptideAtlasiO17680
PRIDEiO17680

2D gel databases

World-2DPAGEi0011:O17680
0020:O17680

Expressioni

Gene expression databases

BgeeiWBGene00008205

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiO17680, 4 interactors
MINTiO17680
STRINGi6239.C49F5.1.2

Structurei

3D structure databases

ProteinModelPortaliO17680
SMRiO17680
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506 Eukaryota
COG0192 LUCA
GeneTreeiENSGT00500000044811
HOGENOMiHOG000245710
InParanoidiO17680
KOiK00789
OMAiPWEVPKN
OrthoDBiEOG091G08Z2
PhylomeDBiO17680

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O17680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKFLFTSE SVSEGHPDKM CDQISDAVLD AHLKQDPNAK VACETVTKTG
60 70 80 90 100
MVMLCGEITS KAVVDYQVLV RRVIEKIGFT DSSIGFDHKT CNVLVALEQQ
110 120 130 140 150
SPEIAAGVHV NKDGEDVGAG DQGIMFGYAT DETEETMPLT LILSHKINAE
160 170 180 190 200
LHKLRRNGTL PWVRPDSKSQ VTIEYESRHG ATIPLRVHTV VVSTQHSPDV
210 220 230 240 250
TLEKLRETIL ADVIKKVIPA SLLDESTVFH INPCGTFIVG GPMGDAGVTG
260 270 280 290 300
RKIIVDTYGG WGAHGGGAFS GKDPTKVDRS AAYAARWVAT SLVKAGLAKR
310 320 330 340 350
VLVQLSYAIG IAKPISVLVY AFGTSPLTDE ELHQIVEDSF DLTPGKIIKE
360 370 380 390 400
LDLKRPIYEK TAENGHFGHS EFPWEQPKAL KISPALLEKA KGNPIPATSA

IAH
Length:403
Mass (Da):43,582
Last modified:January 1, 1998 - v1
Checksum:i81A54B625BC7D96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81485 Genomic DNA Translation: CAB03975.1
PIRiT20070
RefSeqiNP_510002.1, NM_077601.3
UniGeneiCel.5591

Genome annotation databases

EnsemblMetazoaiC49F5.1.1; C49F5.1.1; WBGene00008205
C49F5.1.2; C49F5.1.2; WBGene00008205
C49F5.1.3; C49F5.1.3; WBGene00008205
GeneIDi181370
KEGGicel:CELE_C49F5.1
UCSCiC49F5.1.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMETK1_CAEEL
AccessioniPrimary (citable) accession number: O17680
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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