Reviewed, UniProtKB/Swiss-Prot O17514 (MES2_CAEEL)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase mes-2
    EC=2.1.1.43
Alternative name(s):
    Maternal-effect sterile protein 2
    E(z) homolog

Gene names

Name:	mes-2
ORF Names:	R06A4.7

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic subunit of a the mes-2/mes-3/mes-6 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. The mes-2/mes-3/mes-6 complex may participate in the global inactivation of the X chromosomes in germline cells. This complex is required to exclude mes-4 from the inactivated X-chromosomes in germline cells. Ref.4 Ref.5
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subunit structure	Interacts directly with mes-6 via its N-terminal domain. Forms a heterotrimeric complex with mes-3 and mes-6. Does not interact with mes-4.
Subcellular location	Nucleus.
Tissue specificity	In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells.
Developmental stage	Expressed both maternally and zygotically. Expressed in all cells of early embryos. In late embryos and L1 larva, it is weakly expressed, while it is expressed at intermediate levels in the germline of L4 larvae. Ref.1
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Developmental protein Methyltransferase Repressor Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	Histone-lysine N-methyltransferase mes-2		PRO_0000213994
Regions
	Domain	620 – 741	122	SET
	Region	1 – 194	194	Interaction with mes-6
	Compositional bias	531 – 600	70	Cys-rich
Experimental info
	Mutagenesis	628 – 629	2	SK → PE in bn48; maternal-effect mutation. Progeny defects in gonad proliferation. Germ cell degeneration. Reduced levels of 'H3-K27Me2' and 'H3-K27Me3'.
	Mutagenesis	674	1	Y → H in bn72; maternal-effect mutation. Progeny defects in gonad proliferation. Germ cell degeneration. Reduced levels of 'H3-K27Me2' and 'H3-K27Me3'. Ref.5 Ref.1
	Sequence conflict	483 – 484	2	RK → VQ in AAC27124. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O17514-1 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: 91ABEBAD94A1D51E	FASTA	773	88,821
	10 20 30 40 50 60 MSNSEPSTST PSGKTKKRGK KCETSMGKSK KSKNLPRFVK IQPIFSSEKI KETVCEQGIE 70 80 90 100 110 120 ECKRMLKGHF NAIKDDYDIR VKDELDTDIK DWLKDASSSV NEYRRRLQEN LGEGRTIAKF 130 140 150 160 170 180 SFKNCEKYEE NDYKVSDSTV TWIKPDRTEE GDLMKKFRAP CSRIEVGDIS PPMIYWVPIE 190 200 210 220 230 240 QSVATPDQLR LTHMPYFGDG IDDGNIYEHL IDMFPDGIHG FSDNWSYVND WILYKLCRAA 250 260 270 280 290 300 LKDYQGSPDV FYYTLYRLWP NKSSQREFSS AFPVLCENFA EKGFDPSSLE PWKKTKIAEG 310 320 330 340 350 360 AQNLRNPTCY ACLAYTCAIH GFKAEIPIEF PNGEFYNAML PLPNNPENDG KMCSGNCWKS 370 380 390 400 410 420 VTMKEVSEVL VPDSEEILQK EVKIYFMKSR IAKMPIEDGA LIVNIYVFNT YIPFCEFVKK 430 440 450 460 470 480 YVDEDDEESK IRSCRDAYHL MMSMAENVSA RRLKMGQPSN RLSIKDRVNN FRRNQLSQEK 490 500 510 520 530 540 AKRKLRHDSL RIQALRDGLD AEKLIREDDM RDSQRNSEKV RMTAVTPITA CRHAGPCNAT 550 560 570 580 590 600 AENCACRENG VCSYMCKCDI NCSQRFPGCN CAAGQCYTKA CQCYRANWEC NPMTCNMCKC 610 620 630 640 650 660 DAIDSNIIKC RNFGMTRMIQ KRTYCGPSKI AGNGLFLLEP AEKDEFITEY TGERISDDEA 670 680 690 700 710 720 ERRGAIYDRY QCSYIFNIET GGAIDSYKIG NLARFANHDS KNPTCYARTM VVAGEHRIGF 730 740 750 760 770 YAKRRLEISE ELTFDYSYSG EHQIAFRMVQ TKERSEKPSR PKSQKLSKPM TSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MES-2, a maternal protein essential for viability of the germline in Caenorhabditis elegans, is homologous to a Drosophila Polycomb group protein." Holdeman R., Nehrt S., Strome S. Development 125:2457-2467(1998) [PubMed: 9609829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF 628-SER-LYS-629 AND TYR-674. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"The Caenorhabditis elegans maternal-effect sterile proteins, MES-2, MES-3, and MES-6, are associated in a complex in embryos." Xu L., Fong Y., Strome S. Proc. Natl. Acad. Sci. U.S.A. 98:5061-5066(2001) [PubMed: 11320248] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH MES-3 AND MES-6.
[4]	"Regulation of the different chromatin states of autosomes and X chromosomes in the germ line of C. elegans." Fong Y., Bender L., Wang W., Strome S. Science 296:2235-2238(2002) [PubMed: 12077420] [Abstract] Cited for: FUNCTION.
[5]	"The MES-2/MES-3/MES-6 complex and regulation of histone H3 methylation in C. elegans." Bender L.B., Cao R., Zhang Y., Strome S. Curr. Biol. 14:1639-1643(2004) [PubMed: 15380065] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH MES-3 AND MES-6, MUTAGENESIS OF 628-SER-LYS-629 AND TYR-674.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011893 mRNA. Translation: AAC27124.1. Z83120, Z81515 Genomic DNA. Translation: CAB05589.2. Z81515, Z83120 Genomic DNA. Translation: CAB04199.2.
PIR	T21436.
RefSeq	NP_496992.3.
UniGene	Cel.19654
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O17514.
Genome annotation databases
Ensembl	R06A4.7; R06A4.7; R06A4.7; Caenorhabditis elegans. [Genome view]
GeneID	175096.
KEGG	cel:R06A4.7.
NMPDR	fig|6239.3.peg.8192.
UCSC	R06A4.7. c. elegans.
Organism-specific databases
CTD	175096.
WormBase	WBGene00003220. mes-2.
WormPep	R06A4.7. CE28067. [WorfDB]
Phylogenomic databases
OMA	RIGFYAK.
Gene expression databases
ArrayExpress	O17514.
Family and domain databases
InterPro	IPR001214. SET. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	886736.

Entry information

Entry name

MES2_CAEEL

Accession

Primary (citable) accession number: O17514
Secondary accession number(s): O62335

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project