O17473 (CATL_BRUPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cathepsin L-like EC=3.4.22.15
Organism	Brugia pahangi (Filarial nematode worm)
Taxonomic identifier	6280 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Brugia

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Potential

Propeptide

17 – 181

165

Activation peptide Potential

PRO_0000026293

Chain

182 – 395

214

Cathepsin L-like

PRO_0000026294

Sites

Active site

206

1

By similarity

Active site

342

1

By similarity

Active site

363

1

By similarity

Amino acid modifications

Disulfide bond

203 ↔ 246

Ref.2

Disulfide bond

237 ↔ 278

Ref.2

Disulfide bond

337 ↔ 385

Ref.2

Secondary structure

1

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395

Helix Strand Turn

Details...

Turn

188 – 192

5

Beta strand

202 – 204

3

Helix

206 – 223

18

Helix

231 – 237

7

Helix

251 – 258

8

Beta strand

259 – 261

3

Turn

266 – 268

3

Beta strand

279 – 283

5

Helix

300 – 310

11

Beta strand

313 – 316

4

Helix

322 – 326

5

Beta strand

343 – 349

7

Beta strand

358 – 362

5

Beta strand

367 – 369

3

Beta strand

374 – 378

5

Helix

384 – 386

3

Sequences

	Sequence		Length	Mass (Da)
	O17473 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6EC65C20333BE2D1	FASTA	395	44,549
	10 20 30 40 50 60 MWTIAQLAVF AILALADFAV AGEIEQLKEV LGKFNKDYKQ GNMTRLASDF RSALKEYGDG 70 80 90 100 110 120 QQGESTVVQE FLKKTEDNGE QRAMEKLETE WKDYVTALGK HYDQKENNFR MAIFESNELM 130 140 150 160 170 180 TERINKKYEQ GLVSYTTALN DLADLTDEEF MVRNGLRLPN QTDLRGKRQT SEFYRYDKSE 190 200 210 220 230 240 RLPDQVDWRT KGAVTPVRNQ GECGSCYAFA TAAALEAYHK QMTGRLLDLS PQNIVDCTRN 250 260 270 280 290 300 LGNNGCSGGY MPTAFQYASR YGIAMESRYP YVGTEQRCRW QQSIAVVTDN GFNEIQPGDE 310 320 330 340 350 360 LALKHAVAKR GPVVVGISGS KRSFRFYKDG VYSEGNCGRP DHAVLAVGYG THPSYGDYWI 370 380 390 VKNSWGTDWG KDGYVYMARN RGNMCHIASA ASFPI

References

[1]	"Cathepsin L-like cysteine protease from Brugia pahangi third stage larvae." Selzer P.M., Huong X., Britton C., McKerrow J.H. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular model of Brugia pahangi third-stage larvae cysteine protease." Selzer P.M., Chen X., Cohen F.E., McKerrow J.H. Submitted (JUL-1998) to the PDB data bank Cited for: 3D-STRUCTURE MODELING OF 182-395, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF031819 mRNA. Translation: AAB86867.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BMU	model	-	A	182-395	[»]

ProteinModelPortal

O17473.

SMR

O17473. Positions 182-395.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]

PANTHER

PTHR12411. PTHR12411. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CATL_BRUPA

Accession

Primary (citable) accession number: O17473

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	October 3, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)