O17214 (FUMH_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable fumarate hydratase, mitochondrial
Short name=Fumarase
EC=4.2.1.2

Gene names

Name:	fum-1
ORF Names:	H14A12.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Subcellular location	Mitochondrion By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrion Inferred from direct assay PubMed 20188671. Source: WormBase tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular_function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 501		Probable fumarate hydratase, mitochondrial		PRO_0000010328
Regions
	Region	167 – 170	4	B site By similarity
	Region	177 – 179	3	Substrate binding By similarity
Sites
	Binding site	138	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O17214 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DA578BE105509C1D	FASTA	501	53,647
	10 20 30 40 50 60 MSAVSMLQGE MLARGGAVIA RGASLATARN FSRTTVPMAK IRKERDTFGE LEVPADKYYG 70 80 90 100 110 120 AQTARSQMNF KIGGPEERMP IPVIHAFGIL KKAAALVNTE FGLDKKLADA ISQAADEVVD 130 140 150 160 170 180 GKLDEHFPLV TWQTGSGTQS NMNVNEVISN RAIEILGGEL GSKKPVHPND HVNMSQSSND 190 200 210 220 230 240 TFPTAMHIAV GREVNSRLLP ALKKLRTALH NKAEEFKDII KIGRTHTQDA VPLTLGQEFS 250 260 270 280 290 300 AYVTQLDNSI ARVESTLPRL YQLAAGGTAV GTGLNTRKGF AEKVAATVSE LTGLPFVTAP 310 320 330 340 350 360 NKFEALAAHD ALVEVHGALN TVAVSFMKIG NDIRFLGSGP RCGLGELSLP ENEPGSSIMP 370 380 390 400 410 420 GKVNPTQCEA ITMVAAQVMG NQVAVSVGGS NGHFELNVFK PLIVRNVLQS TRLLADSAVS 430 440 450 460 470 480 FTDHCVDGIV ANKDNIAKIM RESLMLVTAL NPHIGYDNAA KIAKTAHKNG TTLVQEAVKL 490 500 GILTEEQFAQ WVKPENMLGP K

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081548 Genomic DNA. Translation: CCD72341.1.
PIR	C88508.
RefSeq	NP_498642.1. NM_066241.7.
3D structure databases
ProteinModelPortal	O17214.
SMR	O17214. Positions 42-501.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25418N.
IntAct	O17214. 1 interaction.
MINT	MINT-1047829.
STRING	6239.H14A12.2a.
2D gel databases
World-2DPAGE	0020:O17214.
Proteomic databases
PaxDb	O17214.
PRIDE	O17214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	H14A12.2a; H14A12.2a; H14A12.2.
GeneID	176059.
KEGG	cel:CELE_H14A12.2.
UCSC	H14A12.2a. c. elegans.
Organism-specific databases
CTD	176059.
WormBase	H14A12.2a; CE11580; WBGene00001503; fum-1.
Phylogenomic databases
eggNOG	COG0114.
GeneTree	ENSGT00390000002779.
HOGENOM	HOG000061736.
InParanoid	O17214.
KO	K01679.
OMA	KDTMGEV.
Enzyme and pathway databases
UniPathway	UPA00223; UER01007.
Gene expression databases
ArrayExpress	O17214.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	890934.

Entry information

Entry name

FUMH_CAEEL

Accession

Primary (citable) accession number: O17214

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project