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O17214 (FUMH_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable fumarate hydratase, mitochondrial

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fum-1
ORF Names:H14A12.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_00743.

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfumarate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrion

Inferred from direct assay PubMed 20188671. Source: WormBase

tricarboxylic acid cycle enzyme complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionfumarate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 501Probable fumarate hydratase, mitochondrial HAMAP-Rule MF_00743PRO_0000010328

Regions

Region167 – 1704B site By similarity
Region177 – 1793Substrate binding By similarity

Sites

Binding site1381Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O17214 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: DA578BE105509C1D

FASTA50153,647
        10         20         30         40         50         60 
MSAVSMLQGE MLARGGAVIA RGASLATARN FSRTTVPMAK IRKERDTFGE LEVPADKYYG 

        70         80         90        100        110        120 
AQTARSQMNF KIGGPEERMP IPVIHAFGIL KKAAALVNTE FGLDKKLADA ISQAADEVVD 

       130        140        150        160        170        180 
GKLDEHFPLV TWQTGSGTQS NMNVNEVISN RAIEILGGEL GSKKPVHPND HVNMSQSSND 

       190        200        210        220        230        240 
TFPTAMHIAV GREVNSRLLP ALKKLRTALH NKAEEFKDII KIGRTHTQDA VPLTLGQEFS 

       250        260        270        280        290        300 
AYVTQLDNSI ARVESTLPRL YQLAAGGTAV GTGLNTRKGF AEKVAATVSE LTGLPFVTAP 

       310        320        330        340        350        360 
NKFEALAAHD ALVEVHGALN TVAVSFMKIG NDIRFLGSGP RCGLGELSLP ENEPGSSIMP 

       370        380        390        400        410        420 
GKVNPTQCEA ITMVAAQVMG NQVAVSVGGS NGHFELNVFK PLIVRNVLQS TRLLADSAVS 

       430        440        450        460        470        480 
FTDHCVDGIV ANKDNIAKIM RESLMLVTAL NPHIGYDNAA KIAKTAHKNG TTLVQEAVKL 

       490        500 
GILTEEQFAQ WVKPENMLGP K 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO081548 Genomic DNA. Translation: CCD72341.1.
PIRC88508.
RefSeqNP_498642.1. NM_066241.7.
UniGeneCel.17823.

3D structure databases

ProteinModelPortalO17214.
SMRO17214. Positions 42-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid41267. 6 interactions.
DIPDIP-25418N.
IntActO17214. 3 interactions.
MINTMINT-1047829.
STRING6239.H14A12.2a.

2D gel databases

World-2DPAGE0020:O17214.

Proteomic databases

PaxDbO17214.
PRIDEO17214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaH14A12.2a; H14A12.2a; H14A12.2.
GeneID176059.
KEGGcel:CELE_H14A12.2.
UCSCH14A12.2a. c. elegans.

Organism-specific databases

CTD176059.
WormBaseH14A12.2a; CE11580; WBGene00001503; fum-1.

Phylogenomic databases

eggNOGCOG0114.
HOGENOMHOG000061736.
InParanoidO17214.
KOK01679.
OMARIEKDTM.
OrthoDBEOG75J0MX.
PhylomeDBO17214.

Enzyme and pathway databases

UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
HAMAPMF_00743. FumaraseC.
InterProIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio890934.
PROO17214.

Entry information

Entry nameFUMH_CAEEL
AccessionPrimary (citable) accession number: O17214
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase