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Protein

Probable fumarate hydratase, mitochondrial

Gene

fum-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate = fumarate + H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei138 – 1381SubstrateBy similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: UniProtKB-EC

GO - Biological processi

  1. fumarate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiREACT_287541. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable fumarate hydratase, mitochondrial (EC:4.2.1.2)
Short name:
Fumarase
Gene namesi
Name:fum-1
ORF Names:H14A12.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiH14A12.2a; CE11580; WBGene00001503; fum-1.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: WormBase
  2. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 501Probable fumarate hydratase, mitochondrialPRO_0000010328
Transit peptidei1 – ?MitochondrionSequence Analysis

Proteomic databases

PaxDbiO17214.
PRIDEiO17214.

2D gel databases

World-2DPAGE0020:O17214.

Expressioni

Gene expression databases

ExpressionAtlasiO17214. baseline.

Interactioni

Protein-protein interaction databases

BioGridi41267. 6 interactions.
DIPiDIP-25418N.
IntActiO17214. 3 interactions.
MINTiMINT-1047829.
STRINGi6239.H14A12.2a.

Structurei

3D structure databases

ProteinModelPortaliO17214.
SMRiO17214. Positions 42-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 1704B siteBy similarity
Regioni177 – 1793Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0114.
GeneTreeiENSGT00390000002779.
HOGENOMiHOG000061736.
InParanoidiO17214.
KOiK01679.
OMAiNTPKGYD.
OrthoDBiEOG75J0MX.
PhylomeDBiO17214.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O17214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVSMLQGE MLARGGAVIA RGASLATARN FSRTTVPMAK IRKERDTFGE
60 70 80 90 100
LEVPADKYYG AQTARSQMNF KIGGPEERMP IPVIHAFGIL KKAAALVNTE
110 120 130 140 150
FGLDKKLADA ISQAADEVVD GKLDEHFPLV TWQTGSGTQS NMNVNEVISN
160 170 180 190 200
RAIEILGGEL GSKKPVHPND HVNMSQSSND TFPTAMHIAV GREVNSRLLP
210 220 230 240 250
ALKKLRTALH NKAEEFKDII KIGRTHTQDA VPLTLGQEFS AYVTQLDNSI
260 270 280 290 300
ARVESTLPRL YQLAAGGTAV GTGLNTRKGF AEKVAATVSE LTGLPFVTAP
310 320 330 340 350
NKFEALAAHD ALVEVHGALN TVAVSFMKIG NDIRFLGSGP RCGLGELSLP
360 370 380 390 400
ENEPGSSIMP GKVNPTQCEA ITMVAAQVMG NQVAVSVGGS NGHFELNVFK
410 420 430 440 450
PLIVRNVLQS TRLLADSAVS FTDHCVDGIV ANKDNIAKIM RESLMLVTAL
460 470 480 490 500
NPHIGYDNAA KIAKTAHKNG TTLVQEAVKL GILTEEQFAQ WVKPENMLGP

K
Length:501
Mass (Da):53,647
Last modified:December 31, 1997 - v1
Checksum:iDA578BE105509C1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081548 Genomic DNA. Translation: CCD72341.1.
PIRiC88508.
RefSeqiNP_498642.1. NM_066241.7.
UniGeneiCel.17823.

Genome annotation databases

EnsemblMetazoaiH14A12.2a; H14A12.2a; WBGene00001503.
GeneIDi176059.
KEGGicel:CELE_H14A12.2.
UCSCiH14A12.2a. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081548 Genomic DNA. Translation: CCD72341.1.
PIRiC88508.
RefSeqiNP_498642.1. NM_066241.7.
UniGeneiCel.17823.

3D structure databases

ProteinModelPortaliO17214.
SMRiO17214. Positions 42-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41267. 6 interactions.
DIPiDIP-25418N.
IntActiO17214. 3 interactions.
MINTiMINT-1047829.
STRINGi6239.H14A12.2a.

2D gel databases

World-2DPAGE0020:O17214.

Proteomic databases

PaxDbiO17214.
PRIDEiO17214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiH14A12.2a; H14A12.2a; WBGene00001503.
GeneIDi176059.
KEGGicel:CELE_H14A12.2.
UCSCiH14A12.2a. c. elegans.

Organism-specific databases

CTDi176059.
WormBaseiH14A12.2a; CE11580; WBGene00001503; fum-1.

Phylogenomic databases

eggNOGiCOG0114.
GeneTreeiENSGT00390000002779.
HOGENOMiHOG000061736.
InParanoidiO17214.
KOiK01679.
OMAiNTPKGYD.
OrthoDBiEOG75J0MX.
PhylomeDBiO17214.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.
ReactomeiREACT_287541. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi890934.
PROiO17214.

Gene expression databases

ExpressionAtlasiO17214. baseline.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiFUMH_CAEEL
AccessioniPrimary (citable) accession number: O17214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2003
Last sequence update: December 31, 1997
Last modified: March 31, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.