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Protein

Two pore potassium channel protein sup-9

Gene

sup-9

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium channel involved in coordination of muscle contraction (PubMed:14534247). Activity is regulated by sup-18 (PubMed:24586202).2 Publications

GO - Molecular functioni

  • potassium channel activity Source: UniProtKB
  • potassium ion leak channel activity Source: GO_Central

GO - Biological processi

  • potassium ion transport Source: UniProtKB
  • regulation of muscle contraction Source: UniProtKB
  • stabilization of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-CEL-1299316. TWIK-releated acid-sensitive K+ channel (TASK).
R-CEL-5576886. Phase 4 - resting membrane potential.

Protein family/group databases

TCDBi1.A.1.9.7. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Two pore potassium channel protein sup-9
Alternative name(s):
Suppressor of unc-93 protein 9
n2P38
Gene namesi
Name:sup-9Imported
ORF Names:F34D6.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF34D6.3; CE28297; WBGene00006318; sup-9.

Subcellular locationi

  • Membrane Curated; Multi-pass membrane protein Curated

  • Note: Associated with dense bodies.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Intramembranei80 – 100Pore-forming; Name=Pore-forming 1Sequence analysisAdd BLAST21
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 157CytoplasmicSequence analysisAdd BLAST29
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Intramembranei186 – 206Pore-forming; Name=Pore-forming 2Sequence analysisAdd BLAST21
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 329CytoplasmicSequence analysisAdd BLAST89

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • muscle cell projection membrane Source: WormBase
  • striated muscle dense body Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → I in n2282; loss of function. 1 Publication1
Mutagenesisi22G → E in n213; loss of function. 1 Publication1
Mutagenesisi23A → V in lr129; loss of function. 1 Publication1
Mutagenesisi27D → N in n4265 and n3975; loss of function. Not required for sup-18 mediated regulation. 1 Publication1
Mutagenesisi41V → A in n1472; loss of function. 1 Publication1
Mutagenesisi44V → E in n233; loss of function. 1 Publication1
Mutagenesisi58D → V in n2291; loss of function. 1 Publication1
Mutagenesisi61I → S in lr35; loss of function. 1 Publication1
Mutagenesisi74A → V in n1025; loss of function. 1 Publication1
Mutagenesisi94I → N in n1016; loss of function. 1 Publication1
Mutagenesisi95G → D in n1020 and n2354; loss of function. 1 Publication1
Mutagenesisi100T → I in n3976; loss of function. Not required for sup-18 mediated regulation. 1 Publication1
Mutagenesisi101P → S in n190, n598, n2353 and n2356; loss of function. 1 Publication1
Mutagenesisi103T → I in n2347; loss of function. 1 Publication1
Mutagenesisi106G → E in n1009; loss of function. 1 Publication1
Mutagenesisi106G → R in n2351; loss of function. 1 Publication1
Mutagenesisi109F → S in lr45; loss of function. 1 Publication1
Mutagenesisi119P → S in n2281 and n2345; loss of function. 1 Publication1
Mutagenesisi121G → R in lr100; loss of function. 1 Publication1
Mutagenesisi122L → F in n264 and n3977; loss of function. Not required for sup-18 mediated regulation. 2 Publications1
Mutagenesisi165W → R in lr38; loss of function. 1 Publication1
Mutagenesisi172G → E in n219; loss of function. 1 Publication1
Mutagenesisi172G → R in n223 and n2355; loss of function. 1 Publication1
Mutagenesisi173G → E in n2288; partial loss of function. 1 Publication1
Mutagenesisi173G → R in n2294 and n2296; loss of function. 1 Publication1
Mutagenesisi174A → T in n2359; partial loss of function. 1 Publication1
Mutagenesisi181E → K in n2350; loss of function. 1 Publication1
Mutagenesisi190Y → F in n2352; loss of function. 1 Publication1
Mutagenesisi195T → I in n2278 and n2343; loss of function. 1 Publication1
Mutagenesisi200G → E in n191, n2283 and n2286; loss of function. 1 Publication1
Mutagenesisi202G → D in n2344; loss of function. 1 Publication1
Mutagenesisi202G → S in n1469; loss of function. 1 Publication1
Mutagenesisi203D → A in lr1; loss of function. 1 Publication1
Mutagenesisi203D → N in n2346 and n2349; loss of function. 1 Publication1
Mutagenesisi226F → S in n1557; loss of function. 1 Publication1
Mutagenesisi230G → E in n2176; loss of function. 1 Publication1
Mutagenesisi230G → R in n2348; loss of function. 1 Publication1
Mutagenesisi235S → F in n189 and n2358; loss of function. 1 Publication1
Mutagenesisi236A → M in n2360 and n2361; partial loss of function. 1 Publication1
Mutagenesisi236A → T in n1550, n3310, e2655 and 2661; gain of function, uncoordinated rubber band response in heterozygous mutants, inviable in homozygous mutants. 1 Publication1
Mutagenesisi242V → M in lr142; loss of function. 1 Publication1
Mutagenesisi244R → W in n3935; loss of function. 1 Publication1
Mutagenesisi292S → A in n4259; loss of function. 1 Publication1
Mutagenesisi292S → F in n1435, n3942, n4253 and n4254; loss of function. 1 Publication1
Mutagenesisi293C → A: Loss of function. 1 Publication1
Mutagenesisi294S → A in n4262; loss of function. 1 Publication1
Mutagenesisi295C → A: Loss of function. 1 Publication1
Mutagenesisi296Y → A: Loss of function. 1 Publication1
Mutagenesisi303L → P in n4269; loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017701 – 329Two pore potassium channel protein sup-9Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Glycosylationi182N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO17185.

Expressioni

Tissue specificityi

Low levels along surface of body-wall muscle cells, in vulval and intestinal muscles and, more weakly, in anal depressor and sphincter muscles. Also expressed in a subset of head neurons.2 Publications

Developmental stagei

Expressed in body wall muscles from 3.5-fold stage of embryogenesis with highest levels in late embryos and L1 stage larvae. Lower levels persist to adulthood.1 Publication

Gene expression databases

BgeeiWBGene00006318.

Interactioni

Subunit structurei

May form a complex with the regulatory subunits unc-93 and sup-10.1 Publication

Protein-protein interaction databases

STRINGi6239.F34D6.3.

Structurei

3D structure databases

ProteinModelPortaliO17185.
SMRiO17185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 296May be important for regulation by and/or interaction with sup-101 Publication8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 98Selectivity filterSequence analysis6
Motifi198 – 203Selectivity filterSequence analysis6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4404. Eukaryota.
ENOG410XRZJ. LUCA.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000231463.
InParanoidiO17185.
KOiK04919.
OMAiMTMNTED.
OrthoDBiEOG091G08DH.
PhylomeDBiO17185.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSiPR01333. 2POREKCHANEL.
PR01095. TASKCHANNEL.

Sequencei

Sequence statusi: Complete.

O17185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQNIRTLS LIVCTLTYLL VGAAVFDALE TENEILQRKL VQRVREKLKT
60 70 80 90 100
KYNMSNADYE ILEATIVKSV PHKAGYQWKF SGAFYFATTV ITTIGYGHST
110 120 130 140 150
PMTDAGKVFC MLYALAGIPL GLIMFQSIGE RMNTFAAKLL RFIRRAAGKQ
160 170 180 190 200
PIVTSSDLII FCTGWGGLLI FGGAFMFSSY ENWTYFDAVY YCFVTLTTIG
210 220 230 240 250
FGDYVALQKR GSLQTQPEYV FFSLVFILFG LTVISAAMNL LVLRFLTMNT
260 270 280 290 300
EDERRDEQEA ILAAQGLVRV GDPTADDDFG RLPLSDNVSL ASCSCYQLPD
310 320
EKLRHRHRKH TEPHGGPPTF SGMTTRPKY
Length:329
Mass (Da):36,992
Last modified:October 1, 2001 - v2
Checksum:i338A6D9A577464CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357729 mRNA. Translation: AAQ84518.1.
AF083652 mRNA. Translation: AAC32863.1.
FO081204 Genomic DNA. Translation: CCD69862.1.
PIRiT32347.
T43509.
RefSeqiNP_494333.1. NM_061932.3.
UniGeneiCel.14253.

Genome annotation databases

EnsemblMetazoaiF34D6.3; F34D6.3; WBGene00006318.
GeneIDi173613.
KEGGicel:CELE_F34D6.3.
UCSCiF34D6.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY357729 mRNA. Translation: AAQ84518.1.
AF083652 mRNA. Translation: AAC32863.1.
FO081204 Genomic DNA. Translation: CCD69862.1.
PIRiT32347.
T43509.
RefSeqiNP_494333.1. NM_061932.3.
UniGeneiCel.14253.

3D structure databases

ProteinModelPortaliO17185.
SMRiO17185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F34D6.3.

Protein family/group databases

TCDBi1.A.1.9.7. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiO17185.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF34D6.3; F34D6.3; WBGene00006318.
GeneIDi173613.
KEGGicel:CELE_F34D6.3.
UCSCiF34D6.3. c. elegans.

Organism-specific databases

CTDi173613.
WormBaseiF34D6.3; CE28297; WBGene00006318; sup-9.

Phylogenomic databases

eggNOGiKOG4404. Eukaryota.
ENOG410XRZJ. LUCA.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000231463.
InParanoidiO17185.
KOiK04919.
OMAiMTMNTED.
OrthoDBiEOG091G08DH.
PhylomeDBiO17185.

Enzyme and pathway databases

ReactomeiR-CEL-1299316. TWIK-releated acid-sensitive K+ channel (TASK).
R-CEL-5576886. Phase 4 - resting membrane potential.

Miscellaneous databases

PROiO17185.

Gene expression databases

BgeeiWBGene00006318.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSiPR01333. 2POREKCHANEL.
PR01095. TASKCHANNEL.
ProtoNetiSearch...

Entry informationi

Entry nameiSUP9_CAEEL
AccessioniPrimary (citable) accession number: O17185
Secondary accession number(s): O76795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

Uncoordinated rubber band response is phenocopied by exposure to the unc-49 agonist muscimol.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.