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O17087

- GLD2_CAEEL

UniProt

O17087 - GLD2_CAEEL

Protein

Poly(A) RNA polymerase gld-2

Gene

gld-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. Acts as a regulator of mitosis/meiosis required for progression through meiotic prophase during oogenesis and spermatogenesis and for promotion of the entry into meiosis from the mitotic cell cycle. May act by regulating and activating gld-1 mRNA activity in germline.7 Publications

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi606 – 6061Magnesium or manganese; catalyticCurated
    Metal bindingi608 – 6081Magnesium or manganese; catalyticCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: WormBase
    4. protein binding Source: IntAct

    GO - Biological processi

    1. embryo development ending in birth or egg hatching Source: WormBase
    2. meiotic nuclear division Source: UniProtKB-KW
    3. mRNA processing Source: UniProtKB-KW
    4. nucleus organization Source: WormBase
    5. regulation of cell division Source: WormBase
    6. RNA polyadenylation Source: WormBase

    Keywords - Molecular functioni

    Developmental protein, Transferase

    Keywords - Biological processi

    Meiosis, mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase gld-2 (EC:2.7.7.19)
    Alternative name(s):
    Defective in germ line development protein 2
    Gene namesi
    Name:gld-2
    ORF Names:ZC308.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiZC308.1a; CE32766; WBGene00001596; gld-2.
    ZC308.1b; CE15159; WBGene00001596; gld-2.
    ZC308.1c; CE33255; WBGene00001596; gld-2.
    ZC308.1d; CE33256; WBGene00001596; gld-2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. P granule Source: WormBase
    3. RNA-directed RNA polymerase complex Source: WormBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi608 – 6081D → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi875 – 8751E → K in h292; induces defects in entry into meiosis and abolishes interaction with gld-3. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11131113Poly(A) RNA polymerase gld-2PRO_0000341556Add
    BLAST

    Proteomic databases

    PaxDbiO17087.
    PRIDEiO17087.

    Expressioni

    Tissue specificityi

    Germline-specific.1 Publication

    Developmental stagei

    Abundant in embryos, fourth larval stages and adults.1 Publication

    Interactioni

    Subunit structurei

    Interacts with gld-3.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gld-3Q95ZK72EBI-2420017,EBI-317828

    Protein-protein interaction databases

    BioGridi37791. 1 interaction.
    IntActiO17087. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO17087.
    SMRiO17087. Positions 554-877.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini780 – 81637PAP-associatedAdd
    BLAST

    Domaini

    In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain. RNA-binding is mediated through its interaction with gld-3.

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    InParanoidiO17087.
    KOiK14079.
    OMAiQKMICES.
    OrthoDBiEOG7DRJ2S.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: O17087-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVMAQQQKNA ERNDEHTRRN RSPSVDSVSR VQQQSGGFAF YNQQSNHQYQ     50
    QSHPRRTSFS RDGNTGYYNN HSGNKRQTYN NQRGGRSYNH RGNSNYQQNG 100
    EYSGNQGCVP KYHQRNQNYP QLQPKYSYFQ PHQRPIFNST QGYGTYSVRR 150
    SSPPSPSALS SSTANSTSNR APTQPPILLR HAEPASDKNH QGSDHEQNHD 200
    PKIHLYRSAG TAPGGYTQCP SPYKQPPPQP PSTPSSSDKR IEQQQAEDWP 250
    TRFQHPPPQF RRGQDPMPAS IELQHKTANQ TMPVDIVQTN QQKTVSSYER 300
    AAQFRASASE LPTDSVDAKH PCFANERMQS ALIGISPQLK TQQQSPGIPI 350
    QNEAEASAVM KAMRSFQFHN WPQMSHGSYY PMPYHLENQM RPMKSGDQLP 400
    LNQQNHNLSG FPAFVGKSSL VGSSLNTRNS SEADPEEMPR IMEKLDDEVT 450
    GADHDKTIDE NRRRIHKSQE PRIGTEEKAL NELPRKANRR NSSCSSISSV 500
    SESSSPSALD ESTLTKILPT DNFRGGRGFA SPSPPTSLLS EPLSRMDVLS 550
    EKIWDYHNKV SQTDEMLQRK LHLRDMLYTA ISPVFPLSGL YVVGSSLNGF 600
    GNNSSDMDLC LMITNKDLDQ KNDAVVVLNL ILSTLQYEKF VESQKLILAK 650
    VPILRINFAA PFDDITVDLN ANNSVAIRNT HLLCYYSSYD WRVRPLVSVV 700
    KEWAKRKGIN DANKSSFTSY SLVLMVIHFL QCGPTKVLPN LQQSYPNRFS 750
    NKVDVRTLNV TMALEEVADD IDQSLSEKTT LGELLIGFLD YYANEFNYDR 800
    DAISIRQGRR VERAALAVRP KIHSNSEGDK ETPPPSSSAS TSSIHNGGTP 850
    GIPMHHSISN PHFWRSQWRC VCIEEPFTNS NTAHSIYDEM VFEAIKKAFR 900
    EAHGELQHNH DLDKLMECEP IKASTTNTGA AVFAATYEGE RPLAQQPNTI 950
    ACASLRVLNS IPVSSGPGHY HYQQQSNQNL SRPQRPGSNQ GYQMNNNRGF 1000
    NGNNQQQHQN RRSFNNQSSS NPGNGSTGPR SSRSNENVRD SSRQQNSQKG 1050
    SSGVSVSKEN VASTTGVPVD KKQQNSNRKD DGNRTKRSPM VQSPEPAKTK 1100
    SEKTPMASSN VSQ 1113
    Length:1,113
    Mass (Da):124,612
    Last modified:March 1, 2003 - v2
    Checksum:iEDDB685EFE422F4B
    GO
    Isoform b (identifier: O17087-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-482: MVMAQQQKNA...IGTEEKALNE → MPSSIICDVS...TGNEGVSSWK

    Note: No experimental confirmation available.

    Show »
    Length:871
    Mass (Da):96,841
    Checksum:iD80C8364E41FFF8F
    GO
    Isoform c (identifier: O17087-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-334: MVMAQQQKNA...NERMQSALIG → MPLSTGSIDS...VVEYIKFLER

    Note: No experimental confirmation available.

    Show »
    Length:1,036
    Mass (Da):115,092
    Checksum:i970E49DFDAD1C5A5
    GO
    Isoform d (identifier: O17087-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-334: MVMAQQQKNA...NERMQSALIG → MCWAVPQPAIFYQLMAIDVVEYIKFLER

    Note: No experimental confirmation available.

    Show »
    Length:807
    Mass (Da):90,176
    Checksum:iC7B5080DC23A7243
    GO

    RNA editingi

    Partially edited. RNA editing takes place in most germline-specific transcripts.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti400 – 4001P → L in RNA edited version.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 482482MVMAQ…KALNE → MPSSIICDVSHEGEASGGPS PSSRISNGFSHTVCDLHHNF PIVKNRRKYHNSISTQSSSS TTTSLERISTSTCTSTSSSA SDAIISRAEKDVQTFLVSDE STTGDHEEEEEDDEDGDDES DHNDYDYEDDGERSEHDHEQ TDNDDDEDEIEPFVSSWCFA MGTQRSVMSSVGEKAPKSNV KSMEDWELDVVERAHRESHL AELYADLSWRFYTHPPTSFC LARVFLRHGLTGNEGVSSWK in isoform b. CuratedVSP_034328Add
    BLAST
    Alternative sequencei1 – 334334MVMAQ…SALIG → MPLSTGSIDSGFFGCTTNDS LKKGDIEDPQKTTPLSMTDE EETFSTSSSGFSQSSAPPLG DAILSSSNSTSSYLTLNSGA YPRKHRTRVRRSNRKLKRLP QNMSMRFESSSLFSNTLTES LETASCCVSVAGSSRSSLSS SSNSANASYDDMVIRLYFYF KSLLIAINHQKMICESIAYL VKDFDARWGNSGVRKKACDP KGCLAPIYTSSRNTDGGLDK KLVKPANFPMCWAVPQPAIF YQLMAIDVVEYIKFLER in isoform c. CuratedVSP_034329Add
    BLAST
    Alternative sequencei1 – 334334MVMAQ…SALIG → MCWAVPQPAIFYQLMAIDVV EYIKFLER in isoform d. CuratedVSP_034330Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY125085 mRNA. Translation: AAM94369.1.
    FO081588 Genomic DNA. Translation: CCD72658.1.
    FO081588 Genomic DNA. Translation: CCD72659.1.
    FO081588 Genomic DNA. Translation: CCD72660.1.
    FO081588 Genomic DNA. Translation: CCD72661.1.
    PIRiT32274.
    T32275.
    RefSeqiNP_001021844.1. NM_001026673.1. [O17087-2]
    NP_001021845.1. NM_001026674.1. [O17087-4]
    NP_491841.2. NM_059440.2. [O17087-3]
    NP_491842.2. NM_059441.4. [O17087-1]
    UniGeneiCel.9280.

    Genome annotation databases

    EnsemblMetazoaiZC308.1a; ZC308.1a; WBGene00001596. [O17087-1]
    GeneIDi172338.
    KEGGicel:CELE_ZC308.1.
    UCSCiZC308.1a. c. elegans. [O17087-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY125085 mRNA. Translation: AAM94369.1 .
    FO081588 Genomic DNA. Translation: CCD72658.1 .
    FO081588 Genomic DNA. Translation: CCD72659.1 .
    FO081588 Genomic DNA. Translation: CCD72660.1 .
    FO081588 Genomic DNA. Translation: CCD72661.1 .
    PIRi T32274.
    T32275.
    RefSeqi NP_001021844.1. NM_001026673.1. [O17087-2 ]
    NP_001021845.1. NM_001026674.1. [O17087-4 ]
    NP_491841.2. NM_059440.2. [O17087-3 ]
    NP_491842.2. NM_059441.4. [O17087-1 ]
    UniGenei Cel.9280.

    3D structure databases

    ProteinModelPortali O17087.
    SMRi O17087. Positions 554-877.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 37791. 1 interaction.
    IntActi O17087. 2 interactions.

    Proteomic databases

    PaxDbi O17087.
    PRIDEi O17087.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZC308.1a ; ZC308.1a ; WBGene00001596 . [O17087-1 ]
    GeneIDi 172338.
    KEGGi cel:CELE_ZC308.1.
    UCSCi ZC308.1a. c. elegans. [O17087-1 ]

    Organism-specific databases

    CTDi 172338.
    WormBasei ZC308.1a ; CE32766 ; WBGene00001596 ; gld-2.
    ZC308.1b ; CE15159 ; WBGene00001596 ; gld-2.
    ZC308.1c ; CE33255 ; WBGene00001596 ; gld-2.
    ZC308.1d ; CE33256 ; WBGene00001596 ; gld-2.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    InParanoidi O17087.
    KOi K14079.
    OMAi QKMICES.
    OrthoDBi EOG7DRJ2S.

    Miscellaneous databases

    NextBioi 875101.
    PROi O17087.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans."
      Wang L., Eckmann C.R., Kadyk L.C., Wickens M., Kimble J.
      Nature 419:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH GLD-3, MUTAGENESIS OF ASP-608 AND GLU-875.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Genetic regulation of entry into meiosis in Caenorhabditis elegans."
      Kadyk L.C., Kimble J.
      Development 125:1803-1813(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Control of the proliferation versus meiotic development decision in the C. elegans germline through regulation of GLD-1 protein accumulation."
      Hansen D., Wilson-Berry L., Dang T., Schedl T.
      Development 131:93-104(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline."
      Hansen D., Hubbard E.J.A., Schedl T.
      Dev. Biol. 268:342-357(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans."
      Eckmann C.R., Crittenden S.L., Suh N., Kimble J.
      Genetics 168:147-160(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GLD-3.
    7. "Caenorhabditis elegans atx-2 promotes germline proliferation and the oocyte fate."
      Maine E.M., Hansen D., Springer D., Vought V.E.
      Genetics 168:817-830(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing."
      Wang L., Kimble J., Wickens M.
      RNA 10:1444-1448(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA EDITING.
    9. "EGO-1, a putative RNA-directed RNA polymerase, promotes germline proliferation in parallel with GLP-1/notch signaling and regulates the spatial organization of nuclear pore complexes and germline P granules in Caenorhabditis elegans."
      Vought V.E., Ohmachi M., Lee M.-H., Maine E.M.
      Genetics 170:1121-1132(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ line."
      Suh N., Jedamzik B., Eckmann C.R., Wickens M., Kimble J.
      Proc. Natl. Acad. Sci. U.S.A. 103:15108-15112(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGLD2_CAEEL
    AccessioniPrimary (citable) accession number: O17087
    Secondary accession number(s): Q86S49, Q86S50, Q86S51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3