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O17087 (GLD2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) RNA polymerase gld-2

EC=2.7.7.19
Alternative name(s):
Defective in germ line development protein 2
Gene names
Name:gld-2
ORF Names:ZC308.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1113 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. Acts as a regulator of mitosis/meiosis required for progression through meiotic prophase during oogenesis and spermatogenesis and for promotion of the entry into meiosis from the mitotic cell cycle. May act by regulating and activating gld-1 mRNA activity in germline. Ref.1 Ref.3 Ref.4 Ref.5 Ref.7 Ref.9 Ref.10

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Interacts with gld-3. Ref.1 Ref.6

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Germline-specific. Ref.1

Developmental stage

Abundant in embryos, fourth larval stages and adults. Ref.1

Domain

In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain. RNA-binding is mediated through its interaction with gld-3.

Sequence similarities

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Contains 1 PAP-associated domain.

RNA editing

Edited at position 400.
Partially edited. RNA editing takes place in most germline-specific transcripts. Ref.8

Binary interactions

With

Entry

#Exp.

IntAct

Notes

gld-3Q95ZK72EBI-2420017,EBI-317828

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O17087-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O17087-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-482: MVMAQQQKNA...IGTEEKALNE → MPSSIICDVS...TGNEGVSSWK
Note: No experimental confirmation available.
Isoform c (identifier: O17087-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MPLSTGSIDS...VVEYIKFLER
Note: No experimental confirmation available.
Isoform d (identifier: O17087-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MCWAVPQPAIFYQLMAIDVVEYIKFLER
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11131113Poly(A) RNA polymerase gld-2
PRO_0000341556

Regions

Domain780 – 81637PAP-associated

Sites

Metal binding6061Magnesium or manganese; catalytic Probable
Metal binding6081Magnesium or manganese; catalytic Probable

Natural variations

Alternative sequence1 – 482482MVMAQ…KALNE → MPSSIICDVSHEGEASGGPS PSSRISNGFSHTVCDLHHNF PIVKNRRKYHNSISTQSSSS TTTSLERISTSTCTSTSSSA SDAIISRAEKDVQTFLVSDE STTGDHEEEEEDDEDGDDES DHNDYDYEDDGERSEHDHEQ TDNDDDEDEIEPFVSSWCFA MGTQRSVMSSVGEKAPKSNV KSMEDWELDVVERAHRESHL AELYADLSWRFYTHPPTSFC LARVFLRHGLTGNEGVSSWK in isoform b.
VSP_034328
Alternative sequence1 – 334334MVMAQ…SALIG → MPLSTGSIDSGFFGCTTNDS LKKGDIEDPQKTTPLSMTDE EETFSTSSSGFSQSSAPPLG DAILSSSNSTSSYLTLNSGA YPRKHRTRVRRSNRKLKRLP QNMSMRFESSSLFSNTLTES LETASCCVSVAGSSRSSLSS SSNSANASYDDMVIRLYFYF KSLLIAINHQKMICESIAYL VKDFDARWGNSGVRKKACDP KGCLAPIYTSSRNTDGGLDK KLVKPANFPMCWAVPQPAIF YQLMAIDVVEYIKFLER in isoform c.
VSP_034329
Alternative sequence1 – 334334MVMAQ…SALIG → MCWAVPQPAIFYQLMAIDVV EYIKFLER in isoform d.
VSP_034330
Natural variant4001P → L in RNA edited version.

Experimental info

Mutagenesis6081D → A: Loss of enzyme activity. Ref.1
Mutagenesis8751E → K in h292; induces defects in entry into meiosis and abolishes interaction with gld-3. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified March 1, 2003. Version 2.
Checksum: EDDB685EFE422F4B

FASTA1,113124,612
        10         20         30         40         50         60 
MVMAQQQKNA ERNDEHTRRN RSPSVDSVSR VQQQSGGFAF YNQQSNHQYQ QSHPRRTSFS 

        70         80         90        100        110        120 
RDGNTGYYNN HSGNKRQTYN NQRGGRSYNH RGNSNYQQNG EYSGNQGCVP KYHQRNQNYP 

       130        140        150        160        170        180 
QLQPKYSYFQ PHQRPIFNST QGYGTYSVRR SSPPSPSALS SSTANSTSNR APTQPPILLR 

       190        200        210        220        230        240 
HAEPASDKNH QGSDHEQNHD PKIHLYRSAG TAPGGYTQCP SPYKQPPPQP PSTPSSSDKR 

       250        260        270        280        290        300 
IEQQQAEDWP TRFQHPPPQF RRGQDPMPAS IELQHKTANQ TMPVDIVQTN QQKTVSSYER 

       310        320        330        340        350        360 
AAQFRASASE LPTDSVDAKH PCFANERMQS ALIGISPQLK TQQQSPGIPI QNEAEASAVM 

       370        380        390        400        410        420 
KAMRSFQFHN WPQMSHGSYY PMPYHLENQM RPMKSGDQLP LNQQNHNLSG FPAFVGKSSL 

       430        440        450        460        470        480 
VGSSLNTRNS SEADPEEMPR IMEKLDDEVT GADHDKTIDE NRRRIHKSQE PRIGTEEKAL 

       490        500        510        520        530        540 
NELPRKANRR NSSCSSISSV SESSSPSALD ESTLTKILPT DNFRGGRGFA SPSPPTSLLS 

       550        560        570        580        590        600 
EPLSRMDVLS EKIWDYHNKV SQTDEMLQRK LHLRDMLYTA ISPVFPLSGL YVVGSSLNGF 

       610        620        630        640        650        660 
GNNSSDMDLC LMITNKDLDQ KNDAVVVLNL ILSTLQYEKF VESQKLILAK VPILRINFAA 

       670        680        690        700        710        720 
PFDDITVDLN ANNSVAIRNT HLLCYYSSYD WRVRPLVSVV KEWAKRKGIN DANKSSFTSY 

       730        740        750        760        770        780 
SLVLMVIHFL QCGPTKVLPN LQQSYPNRFS NKVDVRTLNV TMALEEVADD IDQSLSEKTT 

       790        800        810        820        830        840 
LGELLIGFLD YYANEFNYDR DAISIRQGRR VERAALAVRP KIHSNSEGDK ETPPPSSSAS 

       850        860        870        880        890        900 
TSSIHNGGTP GIPMHHSISN PHFWRSQWRC VCIEEPFTNS NTAHSIYDEM VFEAIKKAFR 

       910        920        930        940        950        960 
EAHGELQHNH DLDKLMECEP IKASTTNTGA AVFAATYEGE RPLAQQPNTI ACASLRVLNS 

       970        980        990       1000       1010       1020 
IPVSSGPGHY HYQQQSNQNL SRPQRPGSNQ GYQMNNNRGF NGNNQQQHQN RRSFNNQSSS 

      1030       1040       1050       1060       1070       1080 
NPGNGSTGPR SSRSNENVRD SSRQQNSQKG SSGVSVSKEN VASTTGVPVD KKQQNSNRKD 

      1090       1100       1110 
DGNRTKRSPM VQSPEPAKTK SEKTPMASSN VSQ 

« Hide

Isoform b [UniParc].

Checksum: D80C8364E41FFF8F
Show »

FASTA87196,841
Isoform c [UniParc].

Checksum: 970E49DFDAD1C5A5
Show »

FASTA1,036115,092
Isoform d [UniParc].

Checksum: C7B5080DC23A7243
Show »

FASTA80790,176

References

« Hide 'large scale' references
[1]"A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans."
Wang L., Eckmann C.R., Kadyk L.C., Wickens M., Kimble J.
Nature 419:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH GLD-3, MUTAGENESIS OF ASP-608 AND GLU-875.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Genetic regulation of entry into meiosis in Caenorhabditis elegans."
Kadyk L.C., Kimble J.
Development 125:1803-1813(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Control of the proliferation versus meiotic development decision in the C. elegans germline through regulation of GLD-1 protein accumulation."
Hansen D., Wilson-Berry L., Dang T., Schedl T.
Development 131:93-104(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline."
Hansen D., Hubbard E.J.A., Schedl T.
Dev. Biol. 268:342-357(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans."
Eckmann C.R., Crittenden S.L., Suh N., Kimble J.
Genetics 168:147-160(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GLD-3.
[7]"Caenorhabditis elegans atx-2 promotes germline proliferation and the oocyte fate."
Maine E.M., Hansen D., Springer D., Vought V.E.
Genetics 168:817-830(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing."
Wang L., Kimble J., Wickens M.
RNA 10:1444-1448(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA EDITING.
[9]"EGO-1, a putative RNA-directed RNA polymerase, promotes germline proliferation in parallel with GLP-1/notch signaling and regulates the spatial organization of nuclear pore complexes and germline P granules in Caenorhabditis elegans."
Vought V.E., Ohmachi M., Lee M.-H., Maine E.M.
Genetics 170:1121-1132(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ line."
Suh N., Jedamzik B., Eckmann C.R., Wickens M., Kimble J.
Proc. Natl. Acad. Sci. U.S.A. 103:15108-15112(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY125085 mRNA. Translation: AAM94369.1.
FO081588 Genomic DNA. Translation: CCD72658.1.
FO081588 Genomic DNA. Translation: CCD72659.1.
FO081588 Genomic DNA. Translation: CCD72660.1.
FO081588 Genomic DNA. Translation: CCD72661.1.
PIRT32274.
T32275.
RefSeqNP_001021844.1. NM_001026673.1.
NP_001021845.1. NM_001026674.1.
NP_491841.2. NM_059440.2.
NP_491842.2. NM_059441.4.
UniGeneCel.9280.

3D structure databases

ProteinModelPortalO17087.
SMRO17087. Positions 547-921.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid37791. 1 interaction.
IntActO17087. 2 interactions.

Proteomic databases

PaxDbO17087.
PRIDEO17087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZC308.1a; ZC308.1a; ZC308.1. [O17087-1]
GeneID172338.
KEGGcel:CELE_ZC308.1.
UCSCZC308.1a. c. elegans. [O17087-1]

Organism-specific databases

CTD172338.
WormBaseZC308.1a; CE32766; WBGene00001596; gld-2.
ZC308.1b; CE15159; WBGene00001596; gld-2.
ZC308.1c; CE33255; WBGene00001596; gld-2.
ZC308.1d; CE33256; WBGene00001596; gld-2.

Phylogenomic databases

eggNOGCOG5260.
InParanoidO17087.
KOK14079.
OMAMEDWELD.
OrthoDBEOG7DRJ2S.

Family and domain databases

InterProIPR002058. PAP_assoc.
[Graphical view]
PfamPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio875101.
PROO17087.

Entry information

Entry nameGLD2_CAEEL
AccessionPrimary (citable) accession number: O17087
Secondary accession number(s): Q86S49, Q86S50, Q86S51
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase