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O17087

- GLD2_CAEEL

UniProt

O17087 - GLD2_CAEEL

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Protein

Poly(A) RNA polymerase gld-2

Gene

gld-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. Acts as a regulator of mitosis/meiosis required for progression through meiotic prophase during oogenesis and spermatogenesis and for promotion of the entry into meiosis from the mitotic cell cycle. May act by regulating and activating gld-1 mRNA activity in germline.7 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi606 – 6061Magnesium or manganese; catalyticCurated
Metal bindingi608 – 6081Magnesium or manganese; catalyticCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: WormBase

GO - Biological processi

  1. embryo development ending in birth or egg hatching Source: WormBase
  2. meiotic nuclear division Source: UniProtKB-KW
  3. mRNA processing Source: UniProtKB-KW
  4. nucleus organization Source: WormBase
  5. regulation of cell division Source: WormBase
  6. RNA polyadenylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Meiosis, mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-2 (EC:2.7.7.19)
Alternative name(s):
Defective in germ line development protein 2
Gene namesi
Name:gld-2
ORF Names:ZC308.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiZC308.1a; CE32766; WBGene00001596; gld-2.
ZC308.1b; CE15159; WBGene00001596; gld-2.
ZC308.1c; CE33255; WBGene00001596; gld-2.
ZC308.1d; CE33256; WBGene00001596; gld-2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. P granule Source: WormBase
  3. RNA-directed RNA polymerase complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi608 – 6081D → A: Loss of enzyme activity. 1 Publication
Mutagenesisi875 – 8751E → K in h292; induces defects in entry into meiosis and abolishes interaction with gld-3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11131113Poly(A) RNA polymerase gld-2PRO_0000341556Add
BLAST

Proteomic databases

PaxDbiO17087.
PRIDEiO17087.

Expressioni

Tissue specificityi

Germline-specific.1 Publication

Developmental stagei

Abundant in embryos, fourth larval stages and adults.1 Publication

Interactioni

Subunit structurei

Interacts with gld-3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gld-3Q95ZK72EBI-2420017,EBI-317828

Protein-protein interaction databases

BioGridi37791. 1 interaction.
IntActiO17087. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliO17087.
SMRiO17087. Positions 554-877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini780 – 81637PAP-associatedAdd
BLAST

Domaini

In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain. RNA-binding is mediated through its interaction with gld-3.

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
InParanoidiO17087.
KOiK14079.
OMAiQKMICES.
OrthoDBiEOG7DRJ2S.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform a (identifier: O17087-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMAQQQKNA ERNDEHTRRN RSPSVDSVSR VQQQSGGFAF YNQQSNHQYQ
60 70 80 90 100
QSHPRRTSFS RDGNTGYYNN HSGNKRQTYN NQRGGRSYNH RGNSNYQQNG
110 120 130 140 150
EYSGNQGCVP KYHQRNQNYP QLQPKYSYFQ PHQRPIFNST QGYGTYSVRR
160 170 180 190 200
SSPPSPSALS SSTANSTSNR APTQPPILLR HAEPASDKNH QGSDHEQNHD
210 220 230 240 250
PKIHLYRSAG TAPGGYTQCP SPYKQPPPQP PSTPSSSDKR IEQQQAEDWP
260 270 280 290 300
TRFQHPPPQF RRGQDPMPAS IELQHKTANQ TMPVDIVQTN QQKTVSSYER
310 320 330 340 350
AAQFRASASE LPTDSVDAKH PCFANERMQS ALIGISPQLK TQQQSPGIPI
360 370 380 390 400
QNEAEASAVM KAMRSFQFHN WPQMSHGSYY PMPYHLENQM RPMKSGDQLP
410 420 430 440 450
LNQQNHNLSG FPAFVGKSSL VGSSLNTRNS SEADPEEMPR IMEKLDDEVT
460 470 480 490 500
GADHDKTIDE NRRRIHKSQE PRIGTEEKAL NELPRKANRR NSSCSSISSV
510 520 530 540 550
SESSSPSALD ESTLTKILPT DNFRGGRGFA SPSPPTSLLS EPLSRMDVLS
560 570 580 590 600
EKIWDYHNKV SQTDEMLQRK LHLRDMLYTA ISPVFPLSGL YVVGSSLNGF
610 620 630 640 650
GNNSSDMDLC LMITNKDLDQ KNDAVVVLNL ILSTLQYEKF VESQKLILAK
660 670 680 690 700
VPILRINFAA PFDDITVDLN ANNSVAIRNT HLLCYYSSYD WRVRPLVSVV
710 720 730 740 750
KEWAKRKGIN DANKSSFTSY SLVLMVIHFL QCGPTKVLPN LQQSYPNRFS
760 770 780 790 800
NKVDVRTLNV TMALEEVADD IDQSLSEKTT LGELLIGFLD YYANEFNYDR
810 820 830 840 850
DAISIRQGRR VERAALAVRP KIHSNSEGDK ETPPPSSSAS TSSIHNGGTP
860 870 880 890 900
GIPMHHSISN PHFWRSQWRC VCIEEPFTNS NTAHSIYDEM VFEAIKKAFR
910 920 930 940 950
EAHGELQHNH DLDKLMECEP IKASTTNTGA AVFAATYEGE RPLAQQPNTI
960 970 980 990 1000
ACASLRVLNS IPVSSGPGHY HYQQQSNQNL SRPQRPGSNQ GYQMNNNRGF
1010 1020 1030 1040 1050
NGNNQQQHQN RRSFNNQSSS NPGNGSTGPR SSRSNENVRD SSRQQNSQKG
1060 1070 1080 1090 1100
SSGVSVSKEN VASTTGVPVD KKQQNSNRKD DGNRTKRSPM VQSPEPAKTK
1110
SEKTPMASSN VSQ
Length:1,113
Mass (Da):124,612
Last modified:March 1, 2003 - v2
Checksum:iEDDB685EFE422F4B
GO
Isoform b (identifier: O17087-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-482: MVMAQQQKNA...IGTEEKALNE → MPSSIICDVS...TGNEGVSSWK

Note: No experimental confirmation available.

Show »
Length:871
Mass (Da):96,841
Checksum:iD80C8364E41FFF8F
GO
Isoform c (identifier: O17087-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MPLSTGSIDS...VVEYIKFLER

Note: No experimental confirmation available.

Show »
Length:1,036
Mass (Da):115,092
Checksum:i970E49DFDAD1C5A5
GO
Isoform d (identifier: O17087-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MCWAVPQPAIFYQLMAIDVVEYIKFLER

Note: No experimental confirmation available.

Show »
Length:807
Mass (Da):90,176
Checksum:iC7B5080DC23A7243
GO

RNA editingi

Partially edited. RNA editing takes place in most germline-specific transcripts.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti400 – 4001P → L in RNA edited version.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 482482MVMAQ…KALNE → MPSSIICDVSHEGEASGGPS PSSRISNGFSHTVCDLHHNF PIVKNRRKYHNSISTQSSSS TTTSLERISTSTCTSTSSSA SDAIISRAEKDVQTFLVSDE STTGDHEEEEEDDEDGDDES DHNDYDYEDDGERSEHDHEQ TDNDDDEDEIEPFVSSWCFA MGTQRSVMSSVGEKAPKSNV KSMEDWELDVVERAHRESHL AELYADLSWRFYTHPPTSFC LARVFLRHGLTGNEGVSSWK in isoform b. CuratedVSP_034328Add
BLAST
Alternative sequencei1 – 334334MVMAQ…SALIG → MPLSTGSIDSGFFGCTTNDS LKKGDIEDPQKTTPLSMTDE EETFSTSSSGFSQSSAPPLG DAILSSSNSTSSYLTLNSGA YPRKHRTRVRRSNRKLKRLP QNMSMRFESSSLFSNTLTES LETASCCVSVAGSSRSSLSS SSNSANASYDDMVIRLYFYF KSLLIAINHQKMICESIAYL VKDFDARWGNSGVRKKACDP KGCLAPIYTSSRNTDGGLDK KLVKPANFPMCWAVPQPAIF YQLMAIDVVEYIKFLER in isoform c. CuratedVSP_034329Add
BLAST
Alternative sequencei1 – 334334MVMAQ…SALIG → MCWAVPQPAIFYQLMAIDVV EYIKFLER in isoform d. CuratedVSP_034330Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY125085 mRNA. Translation: AAM94369.1.
FO081588 Genomic DNA. Translation: CCD72658.1.
FO081588 Genomic DNA. Translation: CCD72659.1.
FO081588 Genomic DNA. Translation: CCD72660.1.
FO081588 Genomic DNA. Translation: CCD72661.1.
PIRiT32274.
T32275.
RefSeqiNP_001021844.1. NM_001026673.1. [O17087-2]
NP_001021845.1. NM_001026674.1. [O17087-4]
NP_491841.2. NM_059440.2. [O17087-3]
NP_491842.2. NM_059441.4. [O17087-1]
UniGeneiCel.9280.

Genome annotation databases

EnsemblMetazoaiZC308.1a; ZC308.1a; WBGene00001596. [O17087-1]
GeneIDi172338.
KEGGicel:CELE_ZC308.1.
UCSCiZC308.1a. c. elegans. [O17087-1]

Keywords - Coding sequence diversityi

Alternative splicing, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY125085 mRNA. Translation: AAM94369.1 .
FO081588 Genomic DNA. Translation: CCD72658.1 .
FO081588 Genomic DNA. Translation: CCD72659.1 .
FO081588 Genomic DNA. Translation: CCD72660.1 .
FO081588 Genomic DNA. Translation: CCD72661.1 .
PIRi T32274.
T32275.
RefSeqi NP_001021844.1. NM_001026673.1. [O17087-2 ]
NP_001021845.1. NM_001026674.1. [O17087-4 ]
NP_491841.2. NM_059440.2. [O17087-3 ]
NP_491842.2. NM_059441.4. [O17087-1 ]
UniGenei Cel.9280.

3D structure databases

ProteinModelPortali O17087.
SMRi O17087. Positions 554-877.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 37791. 1 interaction.
IntActi O17087. 2 interactions.

Proteomic databases

PaxDbi O17087.
PRIDEi O17087.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZC308.1a ; ZC308.1a ; WBGene00001596 . [O17087-1 ]
GeneIDi 172338.
KEGGi cel:CELE_ZC308.1.
UCSCi ZC308.1a. c. elegans. [O17087-1 ]

Organism-specific databases

CTDi 172338.
WormBasei ZC308.1a ; CE32766 ; WBGene00001596 ; gld-2.
ZC308.1b ; CE15159 ; WBGene00001596 ; gld-2.
ZC308.1c ; CE33255 ; WBGene00001596 ; gld-2.
ZC308.1d ; CE33256 ; WBGene00001596 ; gld-2.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
InParanoidi O17087.
KOi K14079.
OMAi QKMICES.
OrthoDBi EOG7DRJ2S.

Miscellaneous databases

NextBioi 875101.
PROi O17087.

Family and domain databases

InterProi IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans."
    Wang L., Eckmann C.R., Kadyk L.C., Wickens M., Kimble J.
    Nature 419:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH GLD-3, MUTAGENESIS OF ASP-608 AND GLU-875.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Genetic regulation of entry into meiosis in Caenorhabditis elegans."
    Kadyk L.C., Kimble J.
    Development 125:1803-1813(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Control of the proliferation versus meiotic development decision in the C. elegans germline through regulation of GLD-1 protein accumulation."
    Hansen D., Wilson-Berry L., Dang T., Schedl T.
    Development 131:93-104(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline."
    Hansen D., Hubbard E.J.A., Schedl T.
    Dev. Biol. 268:342-357(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans."
    Eckmann C.R., Crittenden S.L., Suh N., Kimble J.
    Genetics 168:147-160(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GLD-3.
  7. "Caenorhabditis elegans atx-2 promotes germline proliferation and the oocyte fate."
    Maine E.M., Hansen D., Springer D., Vought V.E.
    Genetics 168:817-830(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing."
    Wang L., Kimble J., Wickens M.
    RNA 10:1444-1448(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA EDITING.
  9. "EGO-1, a putative RNA-directed RNA polymerase, promotes germline proliferation in parallel with GLP-1/notch signaling and regulates the spatial organization of nuclear pore complexes and germline P granules in Caenorhabditis elegans."
    Vought V.E., Ohmachi M., Lee M.-H., Maine E.M.
    Genetics 170:1121-1132(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ line."
    Suh N., Jedamzik B., Eckmann C.R., Wickens M., Kimble J.
    Proc. Natl. Acad. Sci. U.S.A. 103:15108-15112(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLD2_CAEEL
AccessioniPrimary (citable) accession number: O17087
Secondary accession number(s): Q86S49, Q86S50, Q86S51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3