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Protein

Poly(A) RNA polymerase gld-2

Gene

gld-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. Acts as a regulator of mitosis/meiosis required for progression through meiotic prophase during oogenesis and spermatogenesis and for promotion of the entry into meiosis from the mitotic cell cycle. May act by regulating and activating gld-1 mRNA activity in germline.7 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi606Magnesium or manganese; catalyticCurated1
Metal bindingi608Magnesium or manganese; catalyticCurated1

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • meiotic cell cycle Source: UniProtKB-KW
  • mRNA processing Source: UniProtKB-KW
  • nucleus organization Source: WormBase
  • regulation of cell division Source: WormBase
  • RNA polyadenylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Meiosis, mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.19. 1045.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-2 (EC:2.7.7.19)
Alternative name(s):
Defective in germ line development protein 2
Gene namesi
Name:gld-2
ORF Names:ZC308.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiZC308.1a; CE32766; WBGene00001596; gld-2.
ZC308.1b; CE15159; WBGene00001596; gld-2.
ZC308.1c; CE33255; WBGene00001596; gld-2.
ZC308.1d; CE33256; WBGene00001596; gld-2.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • P granule Source: WormBase
  • protein complex Source: WormBase
  • RNA-directed RNA polymerase complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi608D → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi875E → K in h292; induces defects in entry into meiosis and abolishes interaction with gld-3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003415561 – 1113Poly(A) RNA polymerase gld-2Add BLAST1113

Proteomic databases

EPDiO17087.
PaxDbiO17087.
PeptideAtlasiO17087.
PRIDEiO17087.

Expressioni

Tissue specificityi

Germline-specific.1 Publication

Developmental stagei

Abundant in embryos, fourth larval stages and adults.1 Publication

Gene expression databases

BgeeiWBGene00001596.
ExpressionAtlasiO17087. differential.

Interactioni

Subunit structurei

Interacts with gld-3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
gld-3Q95ZK72EBI-2420017,EBI-317828

Protein-protein interaction databases

BioGridi37791. 1 interactor.
IntActiO17087. 2 interactors.
STRINGi6239.ZC308.1a.

Structurei

Secondary structure

11113
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi548 – 560Combined sources13
Helixi564 – 581Combined sources18
Turni582 – 584Combined sources3
Beta strandi589 – 593Combined sources5
Helixi594 – 596Combined sources3
Beta strandi605 – 612Combined sources8
Turni620 – 622Combined sources3
Helixi623 – 635Combined sources13
Beta strandi641 – 647Combined sources7
Beta strandi649 – 651Combined sources3
Beta strandi653 – 658Combined sources6
Helixi660 – 662Combined sources3
Beta strandi666 – 672Combined sources7
Helixi675 – 686Combined sources12
Turni687 – 689Combined sources3
Helixi693 – 706Combined sources14
Beta strandi712 – 716Combined sources5
Helixi719 – 732Combined sources14
Beta strandi733 – 735Combined sources3
Helixi741 – 744Combined sources4
Turni746 – 748Combined sources3
Beta strandi749 – 753Combined sources5
Helixi755 – 757Combined sources3
Turni773 – 775Combined sources3
Helixi776 – 778Combined sources3
Helixi781 – 791Combined sources11
Helixi795 – 797Combined sources3
Turni798 – 800Combined sources3
Beta strandi801 – 804Combined sources4
Turni805 – 808Combined sources4
Beta strandi809 – 812Combined sources4
Turni861 – 865Combined sources5
Helixi869 – 871Combined sources3
Helixi886 – 909Combined sources24
Helixi912 – 916Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZRLX-ray2.28A528-923[»]
5JNBX-ray2.49A/B/C/D546-923[»]
ProteinModelPortaliO17087.
SMRiO17087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini780 – 816PAP-associatedAdd BLAST37

Domaini

In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain. RNA-binding is mediated through its interaction with gld-3.

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
InParanoidiO17087.
KOiK14079.
OMAiMEDWELD.
OrthoDBiEOG091G0BUD.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O17087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVMAQQQKNA ERNDEHTRRN RSPSVDSVSR VQQQSGGFAF YNQQSNHQYQ
60 70 80 90 100
QSHPRRTSFS RDGNTGYYNN HSGNKRQTYN NQRGGRSYNH RGNSNYQQNG
110 120 130 140 150
EYSGNQGCVP KYHQRNQNYP QLQPKYSYFQ PHQRPIFNST QGYGTYSVRR
160 170 180 190 200
SSPPSPSALS SSTANSTSNR APTQPPILLR HAEPASDKNH QGSDHEQNHD
210 220 230 240 250
PKIHLYRSAG TAPGGYTQCP SPYKQPPPQP PSTPSSSDKR IEQQQAEDWP
260 270 280 290 300
TRFQHPPPQF RRGQDPMPAS IELQHKTANQ TMPVDIVQTN QQKTVSSYER
310 320 330 340 350
AAQFRASASE LPTDSVDAKH PCFANERMQS ALIGISPQLK TQQQSPGIPI
360 370 380 390 400
QNEAEASAVM KAMRSFQFHN WPQMSHGSYY PMPYHLENQM RPMKSGDQLP
410 420 430 440 450
LNQQNHNLSG FPAFVGKSSL VGSSLNTRNS SEADPEEMPR IMEKLDDEVT
460 470 480 490 500
GADHDKTIDE NRRRIHKSQE PRIGTEEKAL NELPRKANRR NSSCSSISSV
510 520 530 540 550
SESSSPSALD ESTLTKILPT DNFRGGRGFA SPSPPTSLLS EPLSRMDVLS
560 570 580 590 600
EKIWDYHNKV SQTDEMLQRK LHLRDMLYTA ISPVFPLSGL YVVGSSLNGF
610 620 630 640 650
GNNSSDMDLC LMITNKDLDQ KNDAVVVLNL ILSTLQYEKF VESQKLILAK
660 670 680 690 700
VPILRINFAA PFDDITVDLN ANNSVAIRNT HLLCYYSSYD WRVRPLVSVV
710 720 730 740 750
KEWAKRKGIN DANKSSFTSY SLVLMVIHFL QCGPTKVLPN LQQSYPNRFS
760 770 780 790 800
NKVDVRTLNV TMALEEVADD IDQSLSEKTT LGELLIGFLD YYANEFNYDR
810 820 830 840 850
DAISIRQGRR VERAALAVRP KIHSNSEGDK ETPPPSSSAS TSSIHNGGTP
860 870 880 890 900
GIPMHHSISN PHFWRSQWRC VCIEEPFTNS NTAHSIYDEM VFEAIKKAFR
910 920 930 940 950
EAHGELQHNH DLDKLMECEP IKASTTNTGA AVFAATYEGE RPLAQQPNTI
960 970 980 990 1000
ACASLRVLNS IPVSSGPGHY HYQQQSNQNL SRPQRPGSNQ GYQMNNNRGF
1010 1020 1030 1040 1050
NGNNQQQHQN RRSFNNQSSS NPGNGSTGPR SSRSNENVRD SSRQQNSQKG
1060 1070 1080 1090 1100
SSGVSVSKEN VASTTGVPVD KKQQNSNRKD DGNRTKRSPM VQSPEPAKTK
1110
SEKTPMASSN VSQ
Length:1,113
Mass (Da):124,612
Last modified:March 1, 2003 - v2
Checksum:iEDDB685EFE422F4B
GO
Isoform b (identifier: O17087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-482: MVMAQQQKNA...IGTEEKALNE → MPSSIICDVS...TGNEGVSSWK

Note: No experimental confirmation available.
Show »
Length:871
Mass (Da):96,841
Checksum:iD80C8364E41FFF8F
GO
Isoform c (identifier: O17087-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MPLSTGSIDS...VVEYIKFLER

Note: No experimental confirmation available.
Show »
Length:1,036
Mass (Da):115,092
Checksum:i970E49DFDAD1C5A5
GO
Isoform d (identifier: O17087-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-334: MVMAQQQKNA...NERMQSALIG → MCWAVPQPAIFYQLMAIDVVEYIKFLER

Note: No experimental confirmation available.
Show »
Length:807
Mass (Da):90,176
Checksum:iC7B5080DC23A7243
GO

RNA editingi

Edited at position 400.1 Publication
Partially edited. RNA editing takes place in most germline-specific transcripts.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti400P → L in RNA edited version. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0343281 – 482MVMAQ…KALNE → MPSSIICDVSHEGEASGGPS PSSRISNGFSHTVCDLHHNF PIVKNRRKYHNSISTQSSSS TTTSLERISTSTCTSTSSSA SDAIISRAEKDVQTFLVSDE STTGDHEEEEEDDEDGDDES DHNDYDYEDDGERSEHDHEQ TDNDDDEDEIEPFVSSWCFA MGTQRSVMSSVGEKAPKSNV KSMEDWELDVVERAHRESHL AELYADLSWRFYTHPPTSFC LARVFLRHGLTGNEGVSSWK in isoform b. CuratedAdd BLAST482
Alternative sequenceiVSP_0343291 – 334MVMAQ…SALIG → MPLSTGSIDSGFFGCTTNDS LKKGDIEDPQKTTPLSMTDE EETFSTSSSGFSQSSAPPLG DAILSSSNSTSSYLTLNSGA YPRKHRTRVRRSNRKLKRLP QNMSMRFESSSLFSNTLTES LETASCCVSVAGSSRSSLSS SSNSANASYDDMVIRLYFYF KSLLIAINHQKMICESIAYL VKDFDARWGNSGVRKKACDP KGCLAPIYTSSRNTDGGLDK KLVKPANFPMCWAVPQPAIF YQLMAIDVVEYIKFLER in isoform c. CuratedAdd BLAST334
Alternative sequenceiVSP_0343301 – 334MVMAQ…SALIG → MCWAVPQPAIFYQLMAIDVV EYIKFLER in isoform d. CuratedAdd BLAST334

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY125085 mRNA. Translation: AAM94369.1.
FO081588 Genomic DNA. Translation: CCD72658.1.
FO081588 Genomic DNA. Translation: CCD72659.1.
FO081588 Genomic DNA. Translation: CCD72660.1.
FO081588 Genomic DNA. Translation: CCD72661.1.
PIRiT32274.
T32275.
RefSeqiNP_001021844.1. NM_001026673.1. [O17087-2]
NP_001021845.1. NM_001026674.1. [O17087-4]
NP_491841.2. NM_059440.2. [O17087-3]
NP_491842.2. NM_059441.4. [O17087-1]
UniGeneiCel.9280.

Genome annotation databases

EnsemblMetazoaiZC308.1a; ZC308.1a; WBGene00001596. [O17087-1]
GeneIDi172338.
KEGGicel:CELE_ZC308.1.
UCSCiZC308.1a. c. elegans. [O17087-1]

Keywords - Coding sequence diversityi

Alternative splicing, RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY125085 mRNA. Translation: AAM94369.1.
FO081588 Genomic DNA. Translation: CCD72658.1.
FO081588 Genomic DNA. Translation: CCD72659.1.
FO081588 Genomic DNA. Translation: CCD72660.1.
FO081588 Genomic DNA. Translation: CCD72661.1.
PIRiT32274.
T32275.
RefSeqiNP_001021844.1. NM_001026673.1. [O17087-2]
NP_001021845.1. NM_001026674.1. [O17087-4]
NP_491841.2. NM_059440.2. [O17087-3]
NP_491842.2. NM_059441.4. [O17087-1]
UniGeneiCel.9280.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZRLX-ray2.28A528-923[»]
5JNBX-ray2.49A/B/C/D546-923[»]
ProteinModelPortaliO17087.
SMRiO17087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37791. 1 interactor.
IntActiO17087. 2 interactors.
STRINGi6239.ZC308.1a.

Proteomic databases

EPDiO17087.
PaxDbiO17087.
PeptideAtlasiO17087.
PRIDEiO17087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZC308.1a; ZC308.1a; WBGene00001596. [O17087-1]
GeneIDi172338.
KEGGicel:CELE_ZC308.1.
UCSCiZC308.1a. c. elegans. [O17087-1]

Organism-specific databases

CTDi172338.
WormBaseiZC308.1a; CE32766; WBGene00001596; gld-2.
ZC308.1b; CE15159; WBGene00001596; gld-2.
ZC308.1c; CE33255; WBGene00001596; gld-2.
ZC308.1d; CE33256; WBGene00001596; gld-2.

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
InParanoidiO17087.
KOiK14079.
OMAiMEDWELD.
OrthoDBiEOG091G0BUD.

Enzyme and pathway databases

BRENDAi2.7.7.19. 1045.

Miscellaneous databases

PROiO17087.

Gene expression databases

BgeeiWBGene00001596.
ExpressionAtlasiO17087. differential.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLD2_CAEEL
AccessioniPrimary (citable) accession number: O17087
Secondary accession number(s): Q86S49, Q86S50, Q86S51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.