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Protein

Zinc metalloproteinase nas-32

Gene

nas-32

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable metalloprotease.By similarity

Catalytic activityi

Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911Zinc; catalyticPROSITE-ProRule annotation
Active sitei292 – 2921PROSITE-ProRule annotation
Metal bindingi295 – 2951Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi301 – 3011Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.A40.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-32 (EC:3.4.24.21)
Alternative name(s):
Nematode astacin 32
Gene namesi
Name:nas-32
ORF Names:T02B11.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT02B11.7; CE50805; WBGene00003550; nas-32.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 651630Zinc metalloproteinase nas-32PRO_0000028936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence analysis
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis
Disulfide bondi393 ↔ 401PROSITE-ProRule annotation
Disulfide bondi395 ↔ 412PROSITE-ProRule annotation
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence analysis
Disulfide bondi415 ↔ 426PROSITE-ProRule annotation
Disulfide bondi434 ↔ 467PROSITE-ProRule annotation
Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence analysis
Disulfide bondi495 ↔ 516PROSITE-ProRule annotation
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi610 ↔ 647PROSITE-ProRule annotation
Disulfide bondi619 ↔ 640PROSITE-ProRule annotation
Disulfide bondi628 ↔ 644PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO16977.
PRIDEiO16977.

Expressioni

Gene expression databases

BgeeiWBGene00003550.

Structurei

3D structure databases

ProteinModelPortaliO16977.
SMRiO16977. Positions 172-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini380 – 43354EGF-likeAdd
BLAST
Domaini434 – 554121CUBAdd
BLAST
Domaini610 – 64738ShKTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 15255Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 1 CUB domain.Curated
Contains 1 EGF-like domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
InParanoidiO16977.
KOiK08076.
OMAiYEYGSFM.
OrthoDBiEOG091G098N.
PhylomeDBiO16977.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O16977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRFFICYIG FLSIFLDFIL ADKDNNSEEE RDRKFDWKFE NENGKPEHET
60 70 80 90 100
VTVPKLPDGS YFWKWTWNSR INSTTAATPT STVTTSTSAP TTSPRVYKLK
110 120 130 140 150
SEARKSLRKA LRGVPPEKRK KQLKKMGKKM MKIPKITKKE SNKLHKSYRK
160 170 180 190 200
VKITENPPAL DMFEVNERAG LNEYLFQGDI NLNNNQIAKI SSEQSSKSRR
210 220 230 240 250
KKRQIDNLAQ FWPGKVVYYY FDSGLTTTVQ QIVRDAITFL ESNTCLKFEL
260 270 280 290 300
NSTATNRIFS GVGCYSDTGM LGGEQTLSLG YGCEVTGTAA HEIAHTLGLF
310 320 330 340 350
HTQMRSDRDD YVTIDLTDVP ESSQQNFIKL TEATSTNLVD YEYGSFMHYS
360 370 380 390 400
GRAFVSSGGV DSIVPKDPVM VYTMGGRIVT FLDLKMLNTH YSCSCPTILS
410 420 430 440 450
CGNGGFTNPA NCSVCICPYG FGGALCTERT DYGCGSTLTA TDTWQQETYT
460 470 480 490 500
FGNASNSATA RPSAVYCNHW IQAPVGKQIQ FRIDSTYNTQ CVYGCTFNGV
510 520 530 540 550
EPKLKSDMTI TQARYCCDEF NAEIMTADFG VNPMPVFSFN RYYKTTYTWS
560 570 580 590 600
YRYVDSNVTA CADTSDKATC LSLKSAKEQG CSIYDTAQLK VMCAATMDLC
610 620 630 640 650
GKVASDDGTC KDRFPKSQCS TYSTNGMCTQ QPPLAAEFSC AETCGFCTNP

V
Length:651
Mass (Da):72,761
Last modified:March 1, 2004 - v3
Checksum:i01982B5BBDEC9FB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081565 Genomic DNA. Translation: CCD72452.1.
AJ561220 mRNA. Translation: CAD99220.1.
PIRiT32205.
RefSeqiNP_503351.3. NM_070950.4.

Genome annotation databases

GeneIDi178595.
KEGGicel:CELE_T02B11.7.
UCSCiT02B11.7. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081565 Genomic DNA. Translation: CCD72452.1.
AJ561220 mRNA. Translation: CAD99220.1.
PIRiT32205.
RefSeqiNP_503351.3. NM_070950.4.

3D structure databases

ProteinModelPortaliO16977.
SMRiO16977. Positions 172-394.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.A40.

Proteomic databases

PaxDbiO16977.
PRIDEiO16977.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi178595.
KEGGicel:CELE_T02B11.7.
UCSCiT02B11.7. c. elegans.

Organism-specific databases

CTDi178595.
WormBaseiT02B11.7; CE50805; WBGene00003550; nas-32.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
InParanoidiO16977.
KOiK08076.
OMAiYEYGSFM.
OrthoDBiEOG091G098N.
PhylomeDBiO16977.

Miscellaneous databases

PROiO16977.

Gene expression databases

BgeeiWBGene00003550.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR017050. Metallopeptidase_nem.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
IPR003582. ShKT_dom.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF01549. ShK. 1 hit.
[Graphical view]
PIRSFiPIRSF036365. Astacin_nematoda. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS51670. SHKT. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAS32_CAEEL
AccessioniPrimary (citable) accession number: O16977
Secondary accession number(s): Q7Z0M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.