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Protein

Zinc metalloproteinase nas-32

Gene

nas-32

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Metalloprotease.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291Zinc; catalyticPROSITE-ProRule annotation1
Active sitei292PROSITE-ProRule annotation1
Metal bindingi295Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi301Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM12.A40

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase nas-32 (EC:3.4.24.-By similarity)
Alternative name(s):
Nematode astacin 32
Gene namesi
Name:nas-32
ORF Names:T02B11.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT02B11.7 ; CE50805 ; WBGene00003550 ; nas-32

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000044267922 – 202By similarityAdd BLAST181
ChainiPRO_0000028936203 – 651Zinc metalloproteinase nas-32Add BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi393 ↔ 401PROSITE-ProRule annotation
Disulfide bondi395 ↔ 412PROSITE-ProRule annotation
Glycosylationi411N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi415 ↔ 426PROSITE-ProRule annotation
Disulfide bondi434 ↔ 467PROSITE-ProRule annotation
Glycosylationi453N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi495 ↔ 516PROSITE-ProRule annotation
Glycosylationi557N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi610 ↔ 647PROSITE-ProRule annotation
Disulfide bondi619 ↔ 640PROSITE-ProRule annotation
Disulfide bondi628 ↔ 644PROSITE-ProRule annotation

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO16977
PRIDEiO16977

Expressioni

Tissue specificityi

Expressed in pharyngeal, anal depressor, intestinal and vulva muscles, head neurons and head mesodermal cell.1 Publication

Gene expression databases

BgeeiWBGene00003550

Structurei

3D structure databases

ProteinModelPortaliO16977
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini380 – 433EGF-likeAdd BLAST54
Domaini434 – 554CUBAdd BLAST121
Domaini610 – 647ShKTPROSITE-ProRule annotationAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 152Lys-richAdd BLAST55

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiKOG3714 Eukaryota
ENOG410ZPX7 LUCA
InParanoidiO16977
KOiK08076
OrthoDBiEOG091G098N
PhylomeDBiO16977

Family and domain databases

CDDicd04280 ZnMc_astacin_like, 1 hit
Gene3Di3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR034035 Astacin-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR017050 Metallopeptidase_nem
IPR001506 Peptidase_M12A
IPR006026 Peptidase_Metallo
IPR003582 ShKT_dom
PfamiView protein in Pfam
PF01400 Astacin, 1 hit
PF01549 ShK, 1 hit
PIRSFiPIRSF036365 Astacin_nematoda, 1 hit
PRINTSiPR00480 ASTACIN
SMARTiView protein in SMART
SM00235 ZnMc, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS51670 SHKT, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O16977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRFFICYIG FLSIFLDFIL ADKDNNSEEE RDRKFDWKFE NENGKPEHET
60 70 80 90 100
VTVPKLPDGS YFWKWTWNSR INSTTAATPT STVTTSTSAP TTSPRVYKLK
110 120 130 140 150
SEARKSLRKA LRGVPPEKRK KQLKKMGKKM MKIPKITKKE SNKLHKSYRK
160 170 180 190 200
VKITENPPAL DMFEVNERAG LNEYLFQGDI NLNNNQIAKI SSEQSSKSRR
210 220 230 240 250
KKRQIDNLAQ FWPGKVVYYY FDSGLTTTVQ QIVRDAITFL ESNTCLKFEL
260 270 280 290 300
NSTATNRIFS GVGCYSDTGM LGGEQTLSLG YGCEVTGTAA HEIAHTLGLF
310 320 330 340 350
HTQMRSDRDD YVTIDLTDVP ESSQQNFIKL TEATSTNLVD YEYGSFMHYS
360 370 380 390 400
GRAFVSSGGV DSIVPKDPVM VYTMGGRIVT FLDLKMLNTH YSCSCPTILS
410 420 430 440 450
CGNGGFTNPA NCSVCICPYG FGGALCTERT DYGCGSTLTA TDTWQQETYT
460 470 480 490 500
FGNASNSATA RPSAVYCNHW IQAPVGKQIQ FRIDSTYNTQ CVYGCTFNGV
510 520 530 540 550
EPKLKSDMTI TQARYCCDEF NAEIMTADFG VNPMPVFSFN RYYKTTYTWS
560 570 580 590 600
YRYVDSNVTA CADTSDKATC LSLKSAKEQG CSIYDTAQLK VMCAATMDLC
610 620 630 640 650
GKVASDDGTC KDRFPKSQCS TYSTNGMCTQ QPPLAAEFSC AETCGFCTNP

V
Length:651
Mass (Da):72,761
Last modified:March 1, 2004 - v3
Checksum:i01982B5BBDEC9FB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081565 Genomic DNA Translation: CCD72452.1
AJ561220 mRNA Translation: CAD99220.1
PIRiT32205
RefSeqiNP_503351.3, NM_070950.4

Genome annotation databases

GeneIDi178595
KEGGicel:CELE_T02B11.7
UCSCiT02B11.7 c. elegans

Similar proteinsi

Entry informationi

Entry nameiNAS32_CAEEL
AccessioniPrimary (citable) accession number: O16977
Secondary accession number(s): Q7Z0M1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2004
Last modified: May 23, 2018
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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