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Protein

3 beta-hydroxysteroid dehydrogenase dhs-16

Gene

dhs-16

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

3 beta-hydroxysteroid dehydrogenase that converts 3 beta-hydroxysteroids to 3-ketosteroids, an essential step in the production of delta(7)-dafachronic acid from cholesterol. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth. Required for longevity in the absence of the germline.1 Publication

Pathwayi: dafachronic acid biosynthesis

This protein is involved in the pathway dafachronic acid biosynthesis, which is part of Steroid hormone biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway dafachronic acid biosynthesis and in Steroid hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • steroid dehydrogenase activity Source: UniProtKB

GO - Biological processi

  • cholesterol metabolic process Source: UniProtKB-KW
  • oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

ReactomeiR-CEL-5365859. RA biosynthesis pathway.
UniPathwayiUPA01020.

Chemistry databases

SwissLipidsiSLP:000000077.
SLP:000000192.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase dhs-16 (EC:1.1.1.-)
Gene namesi
Name:dhs-16
ORF Names:C10F3.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC10F3.2; CE08067; WBGene00000979; dhs-16.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 22HelicalSequence analysisAdd BLAST21
Transmembranei300 – 320HelicalSequence analysisAdd BLAST21
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004216791 – 3883 beta-hydroxysteroid dehydrogenase dhs-16Add BLAST388

Proteomic databases

PaxDbiO16881.

Expressioni

Tissue specificityi

Strongly expressed in the hypodermis and posterior pharyngeal bulb and in a number of unidentified neurons of the head and tail.1 Publication

Gene expression databases

BgeeiWBGene00000979.

Interactioni

Protein-protein interaction databases

STRINGi6239.C10F3.2.

Structurei

3D structure databases

ProteinModelPortaliO16881.
SMRiO16881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00860000133667.
InParanoidiO16881.
OMAiCHAFVPL.
OrthoDBiEOG091G0LMI.
PhylomeDBiO16881.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O16881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELIYILPL LCFVYFLFRR FVLENFYVES SGKYVMITGC DSGFGRLLAT
60 70 80 90 100
SLLDKHVNVF AACFTQQGMA SLHSEWKLKK GPKGQLYTLQ LDVTSQASVD
110 120 130 140 150
SAKSFVTKIL KEQNSKLWGL VNNAGIFSIH GPDDWCSVDE YASSLNVNTL
160 170 180 190 200
GAVRMCHAFV PLIKKSRGRI VTMGSTAGRL HGLYVAPYVT AKFAVEAYMD
210 220 230 240 250
CLRLEMRPFG VSVHILEPGC FKTELLNNDA QRMRIQKIWN SLSVETKEEY
260 270 280 290 300
GEDYRNDFER AWEAGVNVVA NPNIGWVVDC YSHALFSWWP RLRYCPGWDA
310 320 330 340 350
IFMFIPLSIF PTALQDWILA GLYKLNPGPS LTPAVLVKNK KRRSAIQWIQ
360 370 380
FLSQIAIIPL LYTIFFVKNT QKQTVETSTH HHNVTVSE
Length:388
Mass (Da):44,079
Last modified:January 1, 1998 - v1
Checksum:i2C576F267167DEA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080493 Genomic DNA. Translation: CCD64121.1.
PIRiT32153.
RefSeqiNP_504554.1. NM_072153.6.
UniGeneiCel.35961.

Genome annotation databases

EnsemblMetazoaiC10F3.2; C10F3.2; WBGene00000979.
GeneIDi178983.
KEGGicel:CELE_C10F3.2.
UCSCiC10F3.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080493 Genomic DNA. Translation: CCD64121.1.
PIRiT32153.
RefSeqiNP_504554.1. NM_072153.6.
UniGeneiCel.35961.

3D structure databases

ProteinModelPortaliO16881.
SMRiO16881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C10F3.2.

Chemistry databases

SwissLipidsiSLP:000000077.
SLP:000000192.

Proteomic databases

PaxDbiO16881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC10F3.2; C10F3.2; WBGene00000979.
GeneIDi178983.
KEGGicel:CELE_C10F3.2.
UCSCiC10F3.2. c. elegans.

Organism-specific databases

CTDi178983.
WormBaseiC10F3.2; CE08067; WBGene00000979; dhs-16.

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00860000133667.
InParanoidiO16881.
OMAiCHAFVPL.
OrthoDBiEOG091G0LMI.
PhylomeDBiO16881.

Enzyme and pathway databases

UniPathwayiUPA01020.
ReactomeiR-CEL-5365859. RA biosynthesis pathway.

Miscellaneous databases

PROiO16881.

Gene expression databases

BgeeiWBGene00000979.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHS16_CAEEL
AccessioniPrimary (citable) accession number: O16881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.