O16810 (ORC1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Origin recognition complex subunit 1
Short name=DmORC1

Gene names

Name:	Orc1
ORF Names:	CG10667

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Ref.1
Subunit structure	ORC is composed of six subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ORC1 family. Contains 1 BAH domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA amplification Inferred from mutant phenotype. Source: FlyBase DNA-dependent DNA replication initiation Inferred from direct assay. Source: FlyBase cell proliferation Inferred from mutant phenotype. Source: FlyBase
Cellular component	nuclear origin of replication recognition complex Inferred from direct assay. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Origin recognition complex subunit 1		PRO_0000127069
Regions
	Domain	45 – 187	143	BAH
	Nucleotide binding	598 – 605	8	ATP Potential
Amino acid modifications
	Modified residue	293	1	Phosphoserine Ref.6
	Modified residue	368	1	Phosphoserine Ref.5
	Modified residue	419	1	Phosphothreonine Ref.6
	Modified residue	426	1	Phosphoserine Ref.6
	Modified residue	430	1	Phosphoserine Ref.6
	Modified residue	533	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	159	1	A → S in AAC47802. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O16810 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: AACD002DEB10A136	FASTA	924	103,281
	10 20 30 40 50 60 MVNKENARSV GQQVKWIGSQ DELPPVKNLE HKNVYFYQKC IYGPLTLSVG DFILVSNADA 70 80 90 100 110 120 AEPDTVSGCD VARILHMYEL RELTDREPCR AIVQWYSWPK AIPHNKYDDD EVAIDFSLEV 130 140 150 160 170 180 IEEHRPYDND VALGAIYRKC IVLEGTSKTS AEEILKRHAN KLKSTACPMF VSRYRFVKVK 190 200 210 220 230 240 RSYRLIPLEI HLEQPEDNAR PTRSSRKSLT AHRESKRSIS ARHDDTAGNK GSSVEKRRRA 250 260 270 280 290 300 SMAASSSVEF IDVNSFICEN KVSPIKIVGG RSVVRLSEKK NAPEINANYL PASPLTEKNA 310 320 330 340 350 360 KVETPKSRAS AARRNLNLSL DRGADTTADS DCLNYSIVQQ TPDPKTPSND MKIKLRLSER 370 380 390 400 410 420 RRSVRLASMD VDPLSLEEAV QEPNAQGRKR LGVANGDIYH TPTKKSKEPL ESAAATEQTP 430 440 450 460 470 480 STRRKSILKS ATSRLAEGTP RRSIHLSNIV EQRVFEDDEI ISTPKRGRSK KTVQDNDEDY 490 500 510 520 530 540 SPKKSVQKTP TRTRRSSTTT KTATTPSKGI TTATATPMTP SQKMKKIRAG ELSPSMQQRT 550 560 570 580 590 600 DLPAKDSSKS ELQLAREQLH VSVVPKSLPC REREFENIYA FLEGKIQDQC GGCMYVSGVP 610 620 630 640 650 660 GTGKTATVTG VIRTLQRMAK QNELPAFEYL EINGMRLTEP RQAYVQIYKQ LTGKTVSWEQ 670 680 690 700 710 720 AHALLEKRFT TPAPRRVTTV LLVDELDILC NRRQDVVYNL LDWPTKSAAK LVVVTIANTM 730 740 750 760 770 780 DLPERLLMGK VTSRLGLTRL TFQPYSHKQL QEIVTARLGG SETFKGEAVQ LVARKVAAVS 790 800 810 820 830 840 GDARRALDIC RRATEIADTA AVKCVTMLHV QQALAEMIAS AKVQAIRNCS RMEQIFLQAI 850 860 870 880 890 900 AAEVTRTGVE ETTFMGVYQQ VETIAAFMGV TFPPPGRALR LCSKLGAERL IISEHSRNDL 910 920 FQKILLNVSA DDIHYALRVE EMVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes." Pak D.T.S., Pflumm M., Chesnokov I., Huang D.W., Kellum R., Marr J., Romanowski P., Botchan M.R. Cell 91:311-323(1997) [PubMed: 9363940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-293; THR-419; SER-426; SER-430 AND SER-533, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017647 mRNA. Translation: AAC47802.1. AE013599 Genomic DNA. Translation: AAF59236.1. AY094780 mRNA. Translation: AAM11133.1.
RefSeq	NP_477303.1. NM_057955.3.
UniGene	Dm.2266.
3D structure databases
ProteinModelPortal	O16810.
SMR	O16810. Positions 555-923.
ModBase	Search...
Protein-protein interaction databases
STRING	O16810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088959; FBpp0088033; FBgn0022772.
GeneID	35686.
KEGG	dme:Dmel_CG10667.
NMPDR	fig|7227.3.peg.3672.
Organism-specific databases
CTD	4998.
FlyBase	FBgn0022772. Orc1.
Phylogenomic databases
eggNOG	inNOG09177.
GeneTree	EMGT00050000013881.
InParanoid	O16810.
OMA	DELDILC.
OrthoDB	EOG4GTHTT.
PhylomeDB	O16810.
Gene expression databases
Bgee	O16810.
GermOnline	CG10667. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR001025. BAH_dom. IPR015163. Cdc6_C_dom. IPR020793. ORC1. [Graphical view]
KO	K02603.
PANTHER	PTHR10763:SF6. ORC1. 1 hit.
Pfam	PF00004. AAA. 1 hit. PF01426. BAH. 1 hit. PF09079. Cdc6_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00439. BAH. 1 hit. SM01074. Cdc6_C. 1 hit. [Graphical view]
PROSITE	PS51038. BAH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	794721.

Entry information

Entry name

ORC1_DROME

Accession

Primary (citable) accession number: O16810
Secondary accession number(s): Q9V4N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	February 21, 2001
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project