O16797 (RL3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L3

Gene names

Name:	RpL3
ORF Names:	CG4863

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the ribosomal protein L3P family.
Sequence caution	The sequence AAR96131.1 differs from that shown. Reason: Frameshift at position 403.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitotic spindle elongation Inferred from mutant phenotype. Source: FlyBase translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: O16797-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: O16797-4)

Also known as: E;

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Note: No experimental confirmation available.

Isoform C (identifier: O16797-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.
     122-138: WYKSKKKAFTKASKKWT → CSSISLLRELFKSLNVV
     139-416: Missing.

Note: No experimental confirmation available.

Isoform D (identifier: O16797-2)

The sequence of this isoform differs from the canonical sequence as follows:
     124-161: KSKKKAFTKA...KMLRYCKVIR → VSEDHVVVLP...ADTCFLLESG
     162-416: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 416	415	60S ribosomal protein L3		PRO_0000077237
Natural variations
	Alternative sequence	1 – 13	13	Missing in isoform B and isoform C.		VSP_022012
	Alternative sequence	122 – 138	17	WYKSK…SKKWT → CSSISLLRELFKSLNVV in isoform C.		VSP_005714
	Alternative sequence	124 – 161	38	KSKKK…CKVIR → VSEDHVVVLPTPFVAIFFAP GVHTHKMAADTCFLLESG in isoform D.		VSP_005715
	Alternative sequence	139 – 416	278	Missing in isoform C.		VSP_005716
	Alternative sequence	162 – 416	255	Missing in isoform D.		VSP_005717

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 779D843982334AA5	FASTA	416	46,916
	10 20 30 40 50 60 MSHRKFSAPR HGSMAFYPKK RSARHRGKVK AFPKDDASKP VHLTCFIGYK AGMTHIVREA 70 80 90 100 110 120 DRPGSKINKK EVVEAVTVLE TPPMIVVGAV GYIETPFGLR ALVNVWAQHL SEECRRRFYK 130 140 150 160 170 180 NWYKSKKKAF TKASKKWTDD LGKKSIENDF RKMLRYCKVI RVIAHSQIRL IKQRQKKAHV 190 200 210 220 230 240 MEIQLNGGSI EDKVKWAREH LEKPIQVSNV FGQDEMIDCV GVTKGKGFKG VTSRWHTKKL 250 260 270 280 290 300 PRKTHKGLRK VACIGAWHPS RVSTTVARAG QKGYHHRTEI NKKIYRIGAG IHTKDGKVIK 310 320 330 340 350 360 NNASTEYDLT DKSITPMGGF PHYGEVNNDF VMIKGCCIGS KKRIITLRKS LLKHTKRSAL 370 380 390 400 410 EQIKLKFIDT SSKMGHGRFQ TPADKLAFMG PLKKDRLKEE AAATTAAAAA ATTTSA
	Isoform B (E) [UniParc]. Checksum: 880747452B915FE0	FASTA	403	45,436
	10 20 30 40 50 60 MAFYPKKRSA RHRGKVKAFP KDDASKPVHL TCFIGYKAGM THIVREADRP GSKINKKEVV 70 80 90 100 110 120 EAVTVLETPP MIVVGAVGYI ETPFGLRALV NVWAQHLSEE CRRRFYKNWY KSKKKAFTKA 130 140 150 160 170 180 SKKWTDDLGK KSIENDFRKM LRYCKVIRVI AHSQIRLIKQ RQKKAHVMEI QLNGGSIEDK 190 200 210 220 230 240 VKWAREHLEK PIQVSNVFGQ DEMIDCVGVT KGKGFKGVTS RWHTKKLPRK THKGLRKVAC 250 260 270 280 290 300 IGAWHPSRVS TTVARAGQKG YHHRTEINKK IYRIGAGIHT KDGKVIKNNA STEYDLTDKS 310 320 330 340 350 360 ITPMGGFPHY GEVNNDFVMI KGCCIGSKKR IITLRKSLLK HTKRSALEQI KLKFIDTSSK 370 380 390 400 MGHGRFQTPA DKLAFMGPLK KDRLKEEAAA TTAAAAAATT TSA
	Isoform C [UniParc]. Checksum: 4EC10DB7B64DB666	FASTA	125	14,132
	10 20 30 40 50 60 MAFYPKKRSA RHRGKVKAFP KDDASKPVHL TCFIGYKAGM THIVREADRP GSKINKKEVV 70 80 90 100 110 120 EAVTVLETPP MIVVGAVGYI ETPFGLRALV NVWAQHLSEE CRRRFYKNCS SISLLRELFK SLNVV
	Isoform D [UniParc]. Checksum: 03925456CDC7AED2	FASTA	161	18,136
	10 20 30 40 50 60 MSHRKFSAPR HGSMAFYPKK RSARHRGKVK AFPKDDASKP VHLTCFIGYK AGMTHIVREA 70 80 90 100 110 120 DRPGSKINKK EVVEAVTVLE TPPMIVVGAV GYIETPFGLR ALVNVWAQHL SEECRRRFYK 130 140 150 160 NWYVSEDHVV VLPTPFVAIF FAPGVHTHKM AADTCFLLES G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the gene for Drosophila L3 ribosomal protein." Chan H.Y.E., Zhang Y., Hoheisel J.D., O'Kane C.J. Gene 212:119-125(1998) [PubMed: 9661671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF016835 mRNA. Translation: AAC26144.1. AE014297 Genomic DNA. Translation: AAF54610.2. AE014297 Genomic DNA. Translation: AAF54611.1. AE014297 Genomic DNA. Translation: AAF54612.2. AE014297 Genomic DNA. Translation: AAN13496.1. BT011339 mRNA. Translation: AAR96131.1. Frameshift.
RefSeq	NP_524316.1. NM_079592.3. NP_731547.1. NM_169377.2. NP_731550.2. NM_169380.2.
3D structure databases
ProteinModelPortal	O16797.
SMR	O16797. Positions 1-393.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17151N.
IntAct	O16797. 2 interactions.
MINT	MINT-277369.
STRING	O16797.
Proteomic databases
PRIDE	O16797.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0082346; FBpp0081822; FBgn0020910.
GeneID	41347.
KEGG	dme:Dmel_CG4863.
Organism-specific databases
CTD	6122.
FlyBase	FBgn0020910. RpL3.
Phylogenomic databases
eggNOG	inNOG06868.
GeneTree	EMGT00050000009023.
InParanoid	O16797.
OMA	KVGQETH.
OrthoDB	EOG4NCJV4.
PhylomeDB	O16797.
Gene expression databases
ArrayExpress	O16797.
Bgee	O16797.
GermOnline	CG4863. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000597. Ribosomal_L3. IPR019926. Ribosomal_L3_CS. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
KO	K02925.
Pfam	PF00297. Ribosomal_L3. 1 hit. [Graphical view]
SUPFAM	SSF50447. Translat_factor. 1 hit.
PROSITE	PS00474. RIBOSOMAL_L3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	823392.

Entry information

Entry name

RL3_DROME

Accession

Primary (citable) accession number: O16797
Secondary accession number(s): Q0KI83 , Q6NNE9, Q9VGR4, Q9VGR5, Q9VGR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project