ID   GLCM2_CAEEL             Reviewed;         516 AA.
AC   O16581;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 2.
DT   27-MAR-2024, entry version 143.
DE   RecName: Full=Putative glucosylceramidase 2;
DE            EC=3.2.1.45;
DE   Flags: Precursor;
GN   Name=gba-2; ORFNames=C33C12.8;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC   -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family. {ECO:0000305}.
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DR   EMBL; FO080754; CCD66456.1; -; Genomic_DNA.
DR   RefSeq; NP_494208.2; NM_061807.5.
DR   AlphaFoldDB; O16581; -.
DR   SMR; O16581; -.
DR   BioGRID; 47987; 3.
DR   DIP; DIP-25825N; -.
DR   STRING; 6239.C33C12.8.1; -.
DR   CAZy; GH30; Glycoside Hydrolase Family 30.
DR   PaxDb; 6239-C33C12-8; -.
DR   PeptideAtlas; O16581; -.
DR   EnsemblMetazoa; C33C12.8.1; C33C12.8.1; WBGene00016340.
DR   GeneID; 183155; -.
DR   KEGG; cel:CELE_C33C12.8; -.
DR   UCSC; C33C12.8; c. elegans.
DR   AGR; WB:WBGene00016340; -.
DR   WormBase; C33C12.8; CE41877; WBGene00016340; gba-2.
DR   eggNOG; KOG2566; Eukaryota.
DR   GeneTree; ENSGT00390000009464; -.
DR   HOGENOM; CLU_014379_1_2_1; -.
DR   InParanoid; O16581; -.
DR   OMA; WIDWNII; -.
DR   OrthoDB; 3473901at2759; -.
DR   PhylomeDB; O16581; -.
DR   UniPathway; UPA00222; -.
DR   PRO; PR:O16581; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00016340; Expressed in adult organism and 1 other cell type or tissue.
DR   GO; GO:0004348; F:glucosylceramidase activity; IBA:GO_Central.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IBA:GO_Central.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR033452; GH30_C.
DR   InterPro; IPR001139; Glyco_hydro_30.
DR   InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR   PANTHER; PTHR11069:SF39; GLUCOSYLCERAMIDASE 2-RELATED; 1.
DR   Pfam; PF02055; Glyco_hydro_30; 1.
DR   Pfam; PF17189; Glyco_hydro_30C; 1.
DR   PRINTS; PR00843; GLHYDRLASE30.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid metabolism; Reference proteome; Signal;
KW   Sphingolipid metabolism.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..516
FT                   /note="Putative glucosylceramidase 2"
FT                   /id="PRO_0000421454"
FT   ACT_SITE        254
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        358
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   516 AA;  57783 MW;  D7A02EB80C218ED6 CRC64;
     MSIAWSCFVL GLFALASLQV ALANDCAQKT FKTGIVCVCN ITYCDEIPDI NLLSGQAATF
     TTSKSGARLH RDVVYATNSD PLTSMHFTID SSKTYQTIQG FGSTFSDASG ANLKSLPDQM
     ADTILRQYFS DSGLNLQFGR VPIASNDFSS RVYTYDDNLE DYNMAHFSLQ REDYQWKIPY
     MQMAQKYNHD LKFFAVPWSA PGWLKTTNST KGYGILLGTN QDTYHKSYVT YILHFLEEYQ
     KNGILFWGLS TQNEPTSGSD KKTKMQSMGF TAEFQRDFIK LDIGPALKSS NAGKNVKILI
     LDDNRGNLPK WADTVLNDKD AASYVSGIAV HSYQDDESDK HLTQTHNNHP DVFIFGTEAS
     EGSKSKDVDY GSFDRAEDYV SDILDDFNNW VTGWTERNLV LDAQGGPSWV SGFADAPVIA
     FPALAQFYKQ PMFYAIAHFS HFLKPGAVRI DHSLNMPNPE IERSAFLNPD GSKVVVLHNK
     NPLAPYSLSI KDTMKSTDHY QVHLSPKTIV TLYIQN
//