Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative glucosylceramidase 2

Gene

gba-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathway:isphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei254 – 2541Proton donorBy similarity
Active sitei358 – 3581NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 2 (EC:3.2.1.45)
Gene namesi
Name:gba-2
ORF Names:C33C12.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC33C12.8; CE41877; WBGene00016340; gba-2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 516493Putative glucosylceramidase 2PRO_0000421454Add
BLAST

Proteomic databases

PaxDbiO16581.
PRIDEiO16581.

Interactioni

Protein-protein interaction databases

BioGridi47987. 3 interactions.
DIPiDIP-25825N.
MINTiMINT-1040113.
STRINGi6239.C33C12.8.

Structurei

3D structure databases

ProteinModelPortaliO16581.
SMRiO16581. Positions 22-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiO16581.
KOiK01201.
OMAiIPYMQMA.
OrthoDBiEOG76DTRZ.
PhylomeDBiO16581.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O16581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIAWSCFVL GLFALASLQV ALANDCAQKT FKTGIVCVCN ITYCDEIPDI
60 70 80 90 100
NLLSGQAATF TTSKSGARLH RDVVYATNSD PLTSMHFTID SSKTYQTIQG
110 120 130 140 150
FGSTFSDASG ANLKSLPDQM ADTILRQYFS DSGLNLQFGR VPIASNDFSS
160 170 180 190 200
RVYTYDDNLE DYNMAHFSLQ REDYQWKIPY MQMAQKYNHD LKFFAVPWSA
210 220 230 240 250
PGWLKTTNST KGYGILLGTN QDTYHKSYVT YILHFLEEYQ KNGILFWGLS
260 270 280 290 300
TQNEPTSGSD KKTKMQSMGF TAEFQRDFIK LDIGPALKSS NAGKNVKILI
310 320 330 340 350
LDDNRGNLPK WADTVLNDKD AASYVSGIAV HSYQDDESDK HLTQTHNNHP
360 370 380 390 400
DVFIFGTEAS EGSKSKDVDY GSFDRAEDYV SDILDDFNNW VTGWTERNLV
410 420 430 440 450
LDAQGGPSWV SGFADAPVIA FPALAQFYKQ PMFYAIAHFS HFLKPGAVRI
460 470 480 490 500
DHSLNMPNPE IERSAFLNPD GSKVVVLHNK NPLAPYSLSI KDTMKSTDHY
510
QVHLSPKTIV TLYIQN
Length:516
Mass (Da):57,783
Last modified:February 5, 2008 - v2
Checksum:iD7A02EB80C218ED6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080754 Genomic DNA. Translation: CCD66456.1.
RefSeqiNP_494208.2. NM_061807.5.
UniGeneiCel.15726.

Genome annotation databases

EnsemblMetazoaiC33C12.8; C33C12.8; WBGene00016340.
GeneIDi183155.
KEGGicel:CELE_C33C12.8.
UCSCiC33C12.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080754 Genomic DNA. Translation: CCD66456.1.
RefSeqiNP_494208.2. NM_061807.5.
UniGeneiCel.15726.

3D structure databases

ProteinModelPortaliO16581.
SMRiO16581. Positions 22-492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi47987. 3 interactions.
DIPiDIP-25825N.
MINTiMINT-1040113.
STRINGi6239.C33C12.8.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PaxDbiO16581.
PRIDEiO16581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC33C12.8; C33C12.8; WBGene00016340.
GeneIDi183155.
KEGGicel:CELE_C33C12.8.
UCSCiC33C12.8. c. elegans.

Organism-specific databases

CTDi183155.
WormBaseiC33C12.8; CE41877; WBGene00016340; gba-2.

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiO16581.
KOiK01201.
OMAiIPYMQMA.
OrthoDBiEOG76DTRZ.
PhylomeDBiO16581.

Enzyme and pathway databases

UniPathwayiUPA00222.

Miscellaneous databases

NextBioi920114.
PROiO16581.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiGLCM2_CAEEL
AccessioniPrimary (citable) accession number: O16581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: February 5, 2008
Last modified: June 24, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.