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O16581 (GLCM2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative glucosylceramidase 2

EC=3.2.1.45
Gene names
Name:gba-2
ORF Names:C33C12.8
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathway

Lipid metabolism; sphingolipid metabolism.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   DomainSignal
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

sphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionglucosylceramidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 516493Putative glucosylceramidase 2
PRO_0000421454

Sites

Active site2541Proton donor By similarity
Active site3581Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O16581 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: D7A02EB80C218ED6

FASTA51657,783
        10         20         30         40         50         60 
MSIAWSCFVL GLFALASLQV ALANDCAQKT FKTGIVCVCN ITYCDEIPDI NLLSGQAATF 

        70         80         90        100        110        120 
TTSKSGARLH RDVVYATNSD PLTSMHFTID SSKTYQTIQG FGSTFSDASG ANLKSLPDQM 

       130        140        150        160        170        180 
ADTILRQYFS DSGLNLQFGR VPIASNDFSS RVYTYDDNLE DYNMAHFSLQ REDYQWKIPY 

       190        200        210        220        230        240 
MQMAQKYNHD LKFFAVPWSA PGWLKTTNST KGYGILLGTN QDTYHKSYVT YILHFLEEYQ 

       250        260        270        280        290        300 
KNGILFWGLS TQNEPTSGSD KKTKMQSMGF TAEFQRDFIK LDIGPALKSS NAGKNVKILI 

       310        320        330        340        350        360 
LDDNRGNLPK WADTVLNDKD AASYVSGIAV HSYQDDESDK HLTQTHNNHP DVFIFGTEAS 

       370        380        390        400        410        420 
EGSKSKDVDY GSFDRAEDYV SDILDDFNNW VTGWTERNLV LDAQGGPSWV SGFADAPVIA 

       430        440        450        460        470        480 
FPALAQFYKQ PMFYAIAHFS HFLKPGAVRI DHSLNMPNPE IERSAFLNPD GSKVVVLHNK 

       490        500        510 
NPLAPYSLSI KDTMKSTDHY QVHLSPKTIV TLYIQN 

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080754 Genomic DNA. Translation: CCD66456.1.
RefSeqNP_494208.2. NM_061807.5.
UniGeneCel.15726.

3D structure databases

ProteinModelPortalO16581.
SMRO16581. Positions 22-492.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-25825N.
MINTMINT-1040113.
STRING6239.C33C12.8.

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PaxDbO16581.
PRIDEO16581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC33C12.8; C33C12.8; WBGene00016340.
GeneID183155.
KEGGcel:CELE_C33C12.8.
UCSCC33C12.8. c. elegans.

Organism-specific databases

CTD183155.
WormBaseC33C12.8; CE41877; WBGene00016340; gba-2.

Phylogenomic databases

eggNOGCOG5520.
GeneTreeENSGT00390000009464.
HOGENOMHOG000270031.
InParanoidO16581.
KOK01201.
OMAISESCIG.
OrthoDBEOG76DTRZ.
PhylomeDBO16581.

Enzyme and pathway databases

UniPathwayUPA00222.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio920114.

Entry information

Entry nameGLCM2_CAEEL
AccessionPrimary (citable) accession number: O16581
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: February 5, 2008
Last modified: June 11, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase