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Protein

Putative glucosylceramidase 1

Gene

gba-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei358 – 3581NucleophileBy similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. sphingolipid metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 1 (EC:3.2.1.45)
Gene namesi
Name:gba-1
ORF Names:C33C12.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiC33C12.3a; CE29208; WBGene00016335; gba-1.
C33C12.3b; CE39682; WBGene00016335; gba-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 523500Putative glucosylceramidase 1PRO_0000421453Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-24530N.
MINTiMINT-1038373.
STRINGi6239.C33C12.3a.

Structurei

3D structure databases

ProteinModelPortaliO16580.
SMRiO16580. Positions 23-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiO16580.
KOiK01201.
OMAiEDFQWKI.
OrthoDBiEOG76DTRZ.
PhylomeDBiO16580.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: O16580-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSRFLLKIF IFLAVFGVDS VRAADCTEKT FKTGTVCVCS LDSCDEIPPL
60 70 80 90 100
DITMGQAALY TTSHTGARLH RDVIYATDTE PFGTLHMTID SSKKYQTIQG
110 120 130 140 150
FGSTFSDASG ANLKSLPDKL SDLIMKQYFS DTGLNLQFGR VPIASTDFSG
160 170 180 190 200
RVYSYNDVAN DYSMQNFNLT KEDFQWKIPY IKNAQKYNPN LKLFAAPWAA
210 220 230 240 250
PGWLKTTKEM TGPGALNGKA GDNYHQAYAK YFVRFLEEYG KSGISFWGLS
260 270 280 290 300
TQNQPTLGSD KKNKIQSTLF TAETQRDFIK TDLGPALAAS SSGKDVKLLI
310 320 330 340 350
LDDNRGNLPK WADTVLNDMD AAKYVGGIGV HAYQDGETDN HLDETHKKHP
360 370 380 390 400
NFFILGTEAS EGYGSKDTHV DYGNWDRAAD TASDILDNMN NWMTGWTERN
410 420 430 440 450
LILDALGGPS WVSDYTDAPV IAFPAMAQFY KQPMFYAIAH FSHFIKPGAV
460 470 480 490 500
RIDHSLNVIE LEVETTAFLN PDGSKVIVML NKGSLVSTEH TVVVQDAADS
510 520
RNHYHFTLPH RAITTLYIQT SQF
Length:523
Mass (Da):58,167
Last modified:December 1, 2001 - v2
Checksum:i428C0B2E6AE7AAD2
GO
Isoform b (identifier: O16580-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:470
Mass (Da):52,340
Checksum:i16ABA6A0E1D5212F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5353Missing in isoform b. CuratedVSP_045724Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080754 Genomic DNA. Translation: CCD66449.1.
FO080754 Genomic DNA. Translation: CCD66450.1.
PIRiT31964.
RefSeqiNP_001040750.1. NM_001047285.2. [O16580-1]
NP_001040751.1. NM_001047286.1. [O16580-2]
UniGeneiCel.9090.

Genome annotation databases

EnsemblMetazoaiC33C12.3a; C33C12.3a; WBGene00016335. [O16580-1]
GeneIDi173574.
KEGGicel:CELE_C33C12.3.
UCSCiC33C12.3a. c. elegans. [O16580-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080754 Genomic DNA. Translation: CCD66449.1.
FO080754 Genomic DNA. Translation: CCD66450.1.
PIRiT31964.
RefSeqiNP_001040750.1. NM_001047285.2. [O16580-1]
NP_001040751.1. NM_001047286.1. [O16580-2]
UniGeneiCel.9090.

3D structure databases

ProteinModelPortaliO16580.
SMRiO16580. Positions 23-484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24530N.
MINTiMINT-1038373.
STRINGi6239.C33C12.3a.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC33C12.3a; C33C12.3a; WBGene00016335. [O16580-1]
GeneIDi173574.
KEGGicel:CELE_C33C12.3.
UCSCiC33C12.3a. c. elegans. [O16580-1]

Organism-specific databases

CTDi173574.
WormBaseiC33C12.3a; CE29208; WBGene00016335; gba-1.
C33C12.3b; CE39682; WBGene00016335; gba-1.

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiO16580.
KOiK01201.
OMAiEDFQWKI.
OrthoDBiEOG76DTRZ.
PhylomeDBiO16580.

Enzyme and pathway databases

UniPathwayiUPA00222.

Miscellaneous databases

NextBioi880229.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiGLCM1_CAEEL
AccessioniPrimary (citable) accession number: O16580
Secondary accession number(s): A8DZ32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.