Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O16580 (GLCM1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative glucosylceramidase 1

EC=3.2.1.45
Gene names
Name:gba-1
ORF Names:C33C12.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathway

Lipid metabolism; sphingolipid metabolism.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

sphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionglucosylceramidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O16580-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O16580-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 523500Putative glucosylceramidase 1
PRO_0000421453

Sites

Active site3581Nucleophile By similarity

Amino acid modifications

Glycosylation1681N-linked (GlcNAc...) Ref.2

Natural variations

Alternative sequence1 – 5353Missing in isoform b.
VSP_045724

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified December 1, 2001. Version 2.
Checksum: 428C0B2E6AE7AAD2

FASTA52358,167
        10         20         30         40         50         60 
MKSRFLLKIF IFLAVFGVDS VRAADCTEKT FKTGTVCVCS LDSCDEIPPL DITMGQAALY 

        70         80         90        100        110        120 
TTSHTGARLH RDVIYATDTE PFGTLHMTID SSKKYQTIQG FGSTFSDASG ANLKSLPDKL 

       130        140        150        160        170        180 
SDLIMKQYFS DTGLNLQFGR VPIASTDFSG RVYSYNDVAN DYSMQNFNLT KEDFQWKIPY 

       190        200        210        220        230        240 
IKNAQKYNPN LKLFAAPWAA PGWLKTTKEM TGPGALNGKA GDNYHQAYAK YFVRFLEEYG 

       250        260        270        280        290        300 
KSGISFWGLS TQNQPTLGSD KKNKIQSTLF TAETQRDFIK TDLGPALAAS SSGKDVKLLI 

       310        320        330        340        350        360 
LDDNRGNLPK WADTVLNDMD AAKYVGGIGV HAYQDGETDN HLDETHKKHP NFFILGTEAS 

       370        380        390        400        410        420 
EGYGSKDTHV DYGNWDRAAD TASDILDNMN NWMTGWTERN LILDALGGPS WVSDYTDAPV 

       430        440        450        460        470        480 
IAFPAMAQFY KQPMFYAIAH FSHFIKPGAV RIDHSLNVIE LEVETTAFLN PDGSKVIVML 

       490        500        510        520 
NKGSLVSTEH TVVVQDAADS RNHYHFTLPH RAITTLYIQT SQF 

« Hide

Isoform b [UniParc].

Checksum: 16ABA6A0E1D5212F
Show »

FASTA47052,340

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[2]"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080754 Genomic DNA. Translation: CCD66449.1.
FO080754 Genomic DNA. Translation: CCD66450.1.
PIRT31964.
RefSeqNP_001040750.1. NM_001047285.2.
NP_001040751.1. NM_001047286.1.
UniGeneCel.9090.

3D structure databases

ProteinModelPortalO16580.
SMRO16580. Positions 23-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-24530N.
MINTMINT-1038373.
STRING6239.C33C12.3a.

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC33C12.3a; C33C12.3a; C33C12.3. [O16580-1]
C33C12.3b; C33C12.3b; C33C12.3. [O16580-2]
GeneID173574.
KEGGcel:CELE_C33C12.3.
UCSCC33C12.3a. c. elegans. [O16580-1]

Organism-specific databases

CTD173574.
WormBaseC33C12.3a; CE29208; WBGene00016335; gba-1.
C33C12.3b; CE39682; WBGene00016335; gba-1.

Phylogenomic databases

eggNOGCOG5520.
HOGENOMHOG000270031.
InParanoidO16580.
KOK01201.
OrthoDBEOG76DTRZ.
PhylomeDBO16580.

Enzyme and pathway databases

UniPathwayUPA00222.

Gene expression databases

ArrayExpressO16580.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio880229.

Entry information

Entry nameGLCM1_CAEEL
AccessionPrimary (citable) accession number: O16580
Secondary accession number(s): A8DZ32
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase