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O16580

- GLCM1_CAEEL

UniProt

O16580 - GLCM1_CAEEL

Protein

Putative glucosylceramidase 1

Gene

gba-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei358 – 3581NucleophileBy similarity

    GO - Molecular functioni

    1. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00222.

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative glucosylceramidase 1 (EC:3.2.1.45)
    Gene namesi
    Name:gba-1
    ORF Names:C33C12.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome II

    Organism-specific databases

    WormBaseiC33C12.3a; CE29208; WBGene00016335; gba-1.
    C33C12.3b; CE39682; WBGene00016335; gba-1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 523500Putative glucosylceramidase 1PRO_0000421453Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Gene expression databases

    ArrayExpressiO16580.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-24530N.
    MINTiMINT-1038373.
    STRINGi6239.C33C12.3a.

    Structurei

    3D structure databases

    ProteinModelPortaliO16580.
    SMRiO16580. Positions 23-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5520.
    GeneTreeiENSGT00390000009464.
    HOGENOMiHOG000270031.
    InParanoidiO16580.
    KOiK01201.
    OMAiEDFQWKI.
    OrthoDBiEOG76DTRZ.
    PhylomeDBiO16580.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: O16580-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKSRFLLKIF IFLAVFGVDS VRAADCTEKT FKTGTVCVCS LDSCDEIPPL    50
    DITMGQAALY TTSHTGARLH RDVIYATDTE PFGTLHMTID SSKKYQTIQG 100
    FGSTFSDASG ANLKSLPDKL SDLIMKQYFS DTGLNLQFGR VPIASTDFSG 150
    RVYSYNDVAN DYSMQNFNLT KEDFQWKIPY IKNAQKYNPN LKLFAAPWAA 200
    PGWLKTTKEM TGPGALNGKA GDNYHQAYAK YFVRFLEEYG KSGISFWGLS 250
    TQNQPTLGSD KKNKIQSTLF TAETQRDFIK TDLGPALAAS SSGKDVKLLI 300
    LDDNRGNLPK WADTVLNDMD AAKYVGGIGV HAYQDGETDN HLDETHKKHP 350
    NFFILGTEAS EGYGSKDTHV DYGNWDRAAD TASDILDNMN NWMTGWTERN 400
    LILDALGGPS WVSDYTDAPV IAFPAMAQFY KQPMFYAIAH FSHFIKPGAV 450
    RIDHSLNVIE LEVETTAFLN PDGSKVIVML NKGSLVSTEH TVVVQDAADS 500
    RNHYHFTLPH RAITTLYIQT SQF 523
    Length:523
    Mass (Da):58,167
    Last modified:December 1, 2001 - v2
    Checksum:i428C0B2E6AE7AAD2
    GO
    Isoform b (identifier: O16580-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-53: Missing.

    Show »
    Length:470
    Mass (Da):52,340
    Checksum:i16ABA6A0E1D5212F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5353Missing in isoform b. CuratedVSP_045724Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080754 Genomic DNA. Translation: CCD66449.1.
    FO080754 Genomic DNA. Translation: CCD66450.1.
    PIRiT31964.
    RefSeqiNP_001040750.1. NM_001047285.2. [O16580-1]
    NP_001040751.1. NM_001047286.1. [O16580-2]
    UniGeneiCel.9090.

    Genome annotation databases

    EnsemblMetazoaiC33C12.3a; C33C12.3a; WBGene00016335. [O16580-1]
    GeneIDi173574.
    KEGGicel:CELE_C33C12.3.
    UCSCiC33C12.3a. c. elegans. [O16580-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080754 Genomic DNA. Translation: CCD66449.1 .
    FO080754 Genomic DNA. Translation: CCD66450.1 .
    PIRi T31964.
    RefSeqi NP_001040750.1. NM_001047285.2. [O16580-1 ]
    NP_001040751.1. NM_001047286.1. [O16580-2 ]
    UniGenei Cel.9090.

    3D structure databases

    ProteinModelPortali O16580.
    SMRi O16580. Positions 23-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-24530N.
    MINTi MINT-1038373.
    STRINGi 6239.C33C12.3a.

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C33C12.3a ; C33C12.3a ; WBGene00016335 . [O16580-1 ]
    GeneIDi 173574.
    KEGGi cel:CELE_C33C12.3.
    UCSCi C33C12.3a. c. elegans. [O16580-1 ]

    Organism-specific databases

    CTDi 173574.
    WormBasei C33C12.3a ; CE29208 ; WBGene00016335 ; gba-1.
    C33C12.3b ; CE39682 ; WBGene00016335 ; gba-1.

    Phylogenomic databases

    eggNOGi COG5520.
    GeneTreei ENSGT00390000009464.
    HOGENOMi HOG000270031.
    InParanoidi O16580.
    KOi K01201.
    OMAi EDFQWKI.
    OrthoDBi EOG76DTRZ.
    PhylomeDBi O16580.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .

    Miscellaneous databases

    NextBioi 880229.

    Gene expression databases

    ArrayExpressi O16580.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGLCM1_CAEEL
    AccessioniPrimary (citable) accession number: O16580
    Secondary accession number(s): A8DZ32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3