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O16580

- GLCM1_CAEEL

UniProt

O16580 - GLCM1_CAEEL

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Protein

Putative glucosylceramidase 1

Gene
gba-1, C33C12.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei358 – 3581Nucleophile By similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. sphingolipid metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 1 (EC:3.2.1.45)
Gene namesi
Name:gba-1
ORF Names:C33C12.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome II

Organism-specific databases

WormBaseiC33C12.3a; CE29208; WBGene00016335; gba-1.
C33C12.3b; CE39682; WBGene00016335; gba-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 523500Putative glucosylceramidase 1PRO_0000421453Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi168 – 1681N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

ArrayExpressiO16580.

Interactioni

Protein-protein interaction databases

DIPiDIP-24530N.
MINTiMINT-1038373.
STRINGi6239.C33C12.3a.

Structurei

3D structure databases

ProteinModelPortaliO16580.
SMRiO16580. Positions 23-484.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiO16580.
KOiK01201.
OMAiEDFQWKI.
OrthoDBiEOG76DTRZ.
PhylomeDBiO16580.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: O16580-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKSRFLLKIF IFLAVFGVDS VRAADCTEKT FKTGTVCVCS LDSCDEIPPL    50
DITMGQAALY TTSHTGARLH RDVIYATDTE PFGTLHMTID SSKKYQTIQG 100
FGSTFSDASG ANLKSLPDKL SDLIMKQYFS DTGLNLQFGR VPIASTDFSG 150
RVYSYNDVAN DYSMQNFNLT KEDFQWKIPY IKNAQKYNPN LKLFAAPWAA 200
PGWLKTTKEM TGPGALNGKA GDNYHQAYAK YFVRFLEEYG KSGISFWGLS 250
TQNQPTLGSD KKNKIQSTLF TAETQRDFIK TDLGPALAAS SSGKDVKLLI 300
LDDNRGNLPK WADTVLNDMD AAKYVGGIGV HAYQDGETDN HLDETHKKHP 350
NFFILGTEAS EGYGSKDTHV DYGNWDRAAD TASDILDNMN NWMTGWTERN 400
LILDALGGPS WVSDYTDAPV IAFPAMAQFY KQPMFYAIAH FSHFIKPGAV 450
RIDHSLNVIE LEVETTAFLN PDGSKVIVML NKGSLVSTEH TVVVQDAADS 500
RNHYHFTLPH RAITTLYIQT SQF 523
Length:523
Mass (Da):58,167
Last modified:December 1, 2001 - v2
Checksum:i428C0B2E6AE7AAD2
GO
Isoform b (identifier: O16580-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:470
Mass (Da):52,340
Checksum:i16ABA6A0E1D5212F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5353Missing in isoform b. VSP_045724Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FO080754 Genomic DNA. Translation: CCD66449.1.
FO080754 Genomic DNA. Translation: CCD66450.1.
PIRiT31964.
RefSeqiNP_001040750.1. NM_001047285.2. [O16580-1]
NP_001040751.1. NM_001047286.1. [O16580-2]
UniGeneiCel.9090.

Genome annotation databases

EnsemblMetazoaiC33C12.3a; C33C12.3a; WBGene00016335. [O16580-1]
GeneIDi173574.
KEGGicel:CELE_C33C12.3.
UCSCiC33C12.3a. c. elegans. [O16580-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FO080754 Genomic DNA. Translation: CCD66449.1 .
FO080754 Genomic DNA. Translation: CCD66450.1 .
PIRi T31964.
RefSeqi NP_001040750.1. NM_001047285.2. [O16580-1 ]
NP_001040751.1. NM_001047286.1. [O16580-2 ]
UniGenei Cel.9090.

3D structure databases

ProteinModelPortali O16580.
SMRi O16580. Positions 23-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-24530N.
MINTi MINT-1038373.
STRINGi 6239.C33C12.3a.

Protein family/group databases

CAZyi GH30. Glycoside Hydrolase Family 30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C33C12.3a ; C33C12.3a ; WBGene00016335 . [O16580-1 ]
GeneIDi 173574.
KEGGi cel:CELE_C33C12.3.
UCSCi C33C12.3a. c. elegans. [O16580-1 ]

Organism-specific databases

CTDi 173574.
WormBasei C33C12.3a ; CE29208 ; WBGene00016335 ; gba-1.
C33C12.3b ; CE39682 ; WBGene00016335 ; gba-1.

Phylogenomic databases

eggNOGi COG5520.
GeneTreei ENSGT00390000009464.
HOGENOMi HOG000270031.
InParanoidi O16580.
KOi K01201.
OMAi EDFQWKI.
OrthoDBi EOG76DTRZ.
PhylomeDBi O16580.

Enzyme and pathway databases

UniPathwayi UPA00222 .

Miscellaneous databases

NextBioi 880229.

Gene expression databases

ArrayExpressi O16580.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR11069. PTHR11069. 1 hit.
Pfami PF02055. Glyco_hydro_30. 1 hit.
[Graphical view ]
PRINTSi PR00843. GLHYDRLASE30.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiGLCM1_CAEEL
AccessioniPrimary (citable) accession number: O16580
Secondary accession number(s): A8DZ32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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