O16580 (GLCM1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glucosylceramidase 1
EC=3.2.1.45

Gene names

Name:	gba-1
ORF Names:	C33C12.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.
Pathway	Lipid metabolism; sphingolipid metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lysosome organization Inferred from electronic annotation. Source: InterPro sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	lysosome Inferred from electronic annotation. Source: InterPro
Molecular_function	glucosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O16580-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: O16580-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 523	500	Putative glucosylceramidase 1		PRO_0000421453
Sites
	Active site	358	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	168	1	N-linked (GlcNAc...) Ref.2
Natural variations
	Alternative sequence	1 – 53	53	Missing in isoform b.		VSP_045724

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified December 1, 2001. Version 2. Checksum: 428C0B2E6AE7AAD2	FASTA	523	58,167
	10 20 30 40 50 60 MKSRFLLKIF IFLAVFGVDS VRAADCTEKT FKTGTVCVCS LDSCDEIPPL DITMGQAALY 70 80 90 100 110 120 TTSHTGARLH RDVIYATDTE PFGTLHMTID SSKKYQTIQG FGSTFSDASG ANLKSLPDKL 130 140 150 160 170 180 SDLIMKQYFS DTGLNLQFGR VPIASTDFSG RVYSYNDVAN DYSMQNFNLT KEDFQWKIPY 190 200 210 220 230 240 IKNAQKYNPN LKLFAAPWAA PGWLKTTKEM TGPGALNGKA GDNYHQAYAK YFVRFLEEYG 250 260 270 280 290 300 KSGISFWGLS TQNQPTLGSD KKNKIQSTLF TAETQRDFIK TDLGPALAAS SSGKDVKLLI 310 320 330 340 350 360 LDDNRGNLPK WADTVLNDMD AAKYVGGIGV HAYQDGETDN HLDETHKKHP NFFILGTEAS 370 380 390 400 410 420 EGYGSKDTHV DYGNWDRAAD TASDILDNMN NWMTGWTERN LILDALGGPS WVSDYTDAPV 430 440 450 460 470 480 IAFPAMAQFY KQPMFYAIAH FSHFIKPGAV RIDHSLNVIE LEVETTAFLN PDGSKVIVML 490 500 510 520 NKGSLVSTEH TVVVQDAADS RNHYHFTLPH RAITTLYIQT SQF
	Isoform b [UniParc]. Checksum: 16ABA6A0E1D5212F	FASTA	470	52,340
	10 20 30 40 50 60 MGQAALYTTS HTGARLHRDV IYATDTEPFG TLHMTIDSSK KYQTIQGFGS TFSDASGANL 70 80 90 100 110 120 KSLPDKLSDL IMKQYFSDTG LNLQFGRVPI ASTDFSGRVY SYNDVANDYS MQNFNLTKED 130 140 150 160 170 180 FQWKIPYIKN AQKYNPNLKL FAAPWAAPGW LKTTKEMTGP GALNGKAGDN YHQAYAKYFV 190 200 210 220 230 240 RFLEEYGKSG ISFWGLSTQN QPTLGSDKKN KIQSTLFTAE TQRDFIKTDL GPALAASSSG 250 260 270 280 290 300 KDVKLLILDD NRGNLPKWAD TVLNDMDAAK YVGGIGVHAY QDGETDNHLD ETHKKHPNFF 310 320 330 340 350 360 ILGTEASEGY GSKDTHVDYG NWDRAADTAS DILDNMNNWM TGWTERNLIL DALGGPSWVS 370 380 390 400 410 420 DYTDAPVIAF PAMAQFYKQP MFYAIAHFSH FIKPGAVRID HSLNVIELEV ETTAFLNPDG 430 440 450 460 470 SKVIVMLNKG SLVSTEHTVV VQDAADSRNH YHFTLPHRAI TTLYIQTSQF

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[2]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-168, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080754 Genomic DNA. Translation: CCD66449.1. FO080754 Genomic DNA. Translation: CCD66450.1.
PIR	T31964.
RefSeq	NP_001040750.1. NM_001047285.2. NP_001040751.1. NM_001047286.1.
UniGene	Cel.9090.
3D structure databases
HSSP	HSSP built from PDB template 1OGS based on UniProtKB P04062.
ProteinModelPortal	O16580.
SMR	O16580. Positions 23-484.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24530N.
MINT	MINT-1038373.
STRING	6239.C33C12.3a.
Protein family/group databases
CAZy	GH30. Glycoside Hydrolase Family 30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	C33C12.3a; C33C12.3a; C33C12.3.
GeneID	173574.
KEGG	cel:CELE_C33C12.3.
UCSC	C33C12.3a. c. elegans.
Organism-specific databases
CTD	173574.
WormBase	C33C12.3a; CE29208; WBGene00016335; gba-1. C33C12.3b; CE39682; WBGene00016335; gba-1.
Phylogenomic databases
eggNOG	COG5520.
GeneTree	ENSGT00390000009464.
HOGENOM	HOG000270031.
InParanoid	O16580.
KO	K01201.
OMA	EDFQWKI.
Enzyme and pathway databases
UniPathway	UPA00222.
Gene expression databases
ArrayExpress	O16580.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR013780. Glyco_hydro_13_b. IPR001139. Glyco_hydro_30. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR11069. PTHR11069. 1 hit.
Pfam	PF02055. Glyco_hydro_30. 1 hit. [Graphical view]
PRINTS	PR00843. GLHYDRLASE30.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	880229.

Entry information

Entry name

GLCM1_CAEEL

Accession

Primary (citable) accession number: O16580
Secondary accession number(s): A8DZ32

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2013
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project