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Protein

Eukaryotic peptide chain release factor subunit 1

Gene

erfa-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-CEL-72764. Eukaryotic Translation Termination.
R-CEL-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-CEL-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1By similarity
Short name:
Eukaryotic release factor 1By similarity
Short name:
eRF1By similarity
Gene namesi
Name:erfa-1Imported
Synonyms:etf-1Imported
ORF Names:T05H4.6Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT05H4.6; CE27442; WBGene00020269; erfa-1.
T05H4.6b; CE13279; WBGene00020269; erfa-1.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559Eukaryotic peptide chain release factor subunit 1CuratedPRO_0000143148Add
BLAST

Proteomic databases

EPDiO16520.
PaxDbiO16520.
PRIDEiO16520.

Interactioni

Subunit structurei

Heterodimer of two subunits, one of which binds GTP.By similarity

Protein-protein interaction databases

IntActiO16520. 1 interaction.
STRINGi6239.T05H4.6.1.

Structurei

3D structure databases

ProteinModelPortaliO16520.
SMRiO16520. Positions 15-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

eggNOGiKOG0688. Eukaryota.
COG1503. LUCA.
GeneTreeiENSGT00390000009004.
HOGENOMiHOG000224681.
InParanoidiO16520.
OMAiRYKMKNS.
PhylomeDBiO16520.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.30.960.10. 1 hit.
InterProiIPR005140. eRF1_1_Pelota.
IPR005141. eRF1_2.
IPR005142. eRF1_3.
IPR029064. L30e-like.
IPR004403. Peptide_chain-rel_eRF1/aRF1.
IPR024049. Release_factor_eRF1/aRF1_N.
[Graphical view]
PANTHERiPTHR10113. PTHR10113. 1 hit.
PfamiPF03463. eRF1_1. 1 hit.
PF03464. eRF1_2. 1 hit.
PF03465. eRF1_3. 1 hit.
[Graphical view]
SMARTiSM01194. eRF1_1. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF55481. SSF55481. 1 hit.
TIGRFAMsiTIGR03676. aRF1/eRF1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: O16520-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPAAGATA GGDDAADRNV EMWKIKRLIK SLELARGNGT SMISLIIPPK
60 70 80 90 100
DQVARIQRML AEEYGTASNI KSRVNRLSVL GAITSVQGRL KLYNKVPPNG
110 120 130 140 150
LVVYCGTIMT DEGKEKKVNI DFEPFKAINT SLYLCDNKFH TEALQGLLAD
160 170 180 190 200
DNKFGFIIMD GNGCLFGTLQ GNTREVLHKF TVDLPKKHGR GGQSAVRFAR
210 220 230 240 250
LRNEKRHNYV RKVAENSVEQ FIKNDKVTVA GLILAGSADF KTELGQSDMF
260 270 280 290 300
DQRLQAKMIK TVDIAYGGEN GFNQAIELAA DTLASVKFIQ EKKLIGGYFD
310 320 330 340 350
EISQDTGKYV FGVKDTLAAL EMGAIETLIC WENLDIVRYK MKNSLGEDIL
360 370 380 390 400
LNLRPDEEKD KSHFTDSETG QDMEIIETMP LLEWFANNYK NFGAALEIVT
410 420 430 440 450
DKSQEGAQFV RGFGGIGGLL RYRVDLAHLV SSFFFINLFI NYPALSNYNI
460 470 480 490 500
SLSQSSSSLI AISHTTLYSK FLFHFIEFAP RVPHYCRFVW SVDAFSSAGK
510 520 530 540 550
ASHVCQHTEK ETFHARNEDA IGGLFNRTKV EARATHSHSM TSTLSLPSVD

VSTRNSNDF
Note: No experimental confirmation available.Curated
Length:559
Mass (Da):62,165
Last modified:October 5, 2010 - v2
Checksum:i93F49F4232D958D7
GO
Isoform bImported (identifier: O16520-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-443: LVSSFFFINLFINYP → VDLEDELDNIDLDDY
     444-559: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:443
Mass (Da):49,218
Checksum:iF77E11365B03565D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei429 – 44315LVSSF…FINYP → VDLEDELDNIDLDDY in isoform b. CuratedVSP_057543Add
BLAST
Alternative sequencei444 – 559116Missing in isoform b. CuratedVSP_057544Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081501 Genomic DNA. Translation: CCD72044.1.
FO081501 Genomic DNA. Translation: CDG24149.1.
PIRiT31907.
RefSeqiNP_001024107.2. NM_001028936.4. [O16520-1]
NP_001294675.1. NM_001307746.1. [O16520-2]
UniGeneiCel.8961.

Genome annotation databases

EnsemblMetazoaiT05H4.6a; T05H4.6a; WBGene00020269. [O16520-1]
GeneIDi179028.
KEGGicel:CELE_T05H4.6.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081501 Genomic DNA. Translation: CCD72044.1.
FO081501 Genomic DNA. Translation: CDG24149.1.
PIRiT31907.
RefSeqiNP_001024107.2. NM_001028936.4. [O16520-1]
NP_001294675.1. NM_001307746.1. [O16520-2]
UniGeneiCel.8961.

3D structure databases

ProteinModelPortaliO16520.
SMRiO16520. Positions 15-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO16520. 1 interaction.
STRINGi6239.T05H4.6.1.

Proteomic databases

EPDiO16520.
PaxDbiO16520.
PRIDEiO16520.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05H4.6a; T05H4.6a; WBGene00020269. [O16520-1]
GeneIDi179028.
KEGGicel:CELE_T05H4.6.

Organism-specific databases

CTDi179028.
WormBaseiT05H4.6; CE27442; WBGene00020269; erfa-1.
T05H4.6b; CE13279; WBGene00020269; erfa-1.

Phylogenomic databases

eggNOGiKOG0688. Eukaryota.
COG1503. LUCA.
GeneTreeiENSGT00390000009004.
HOGENOMiHOG000224681.
InParanoidiO16520.
OMAiRYKMKNS.
PhylomeDBiO16520.

Enzyme and pathway databases

ReactomeiR-CEL-72764. Eukaryotic Translation Termination.
R-CEL-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-CEL-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiO16520.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.30.960.10. 1 hit.
InterProiIPR005140. eRF1_1_Pelota.
IPR005141. eRF1_2.
IPR005142. eRF1_3.
IPR029064. L30e-like.
IPR004403. Peptide_chain-rel_eRF1/aRF1.
IPR024049. Release_factor_eRF1/aRF1_N.
[Graphical view]
PANTHERiPTHR10113. PTHR10113. 1 hit.
PfamiPF03463. eRF1_1. 1 hit.
PF03464. eRF1_2. 1 hit.
PF03465. eRF1_3. 1 hit.
[Graphical view]
SMARTiSM01194. eRF1_1. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF55481. SSF55481. 1 hit.
TIGRFAMsiTIGR03676. aRF1/eRF1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiERF1_CAEEL
AccessioniPrimary (citable) accession number: O16520
Secondary accession number(s): S6EZT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 5, 2010
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.