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Protein

Gastrulation defective protein 1

Gene

gad-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for initiation of gastrulation.1 Publication

GO - Biological processi

  • cell migration involved in gastrulation Source: WormBase
  • establishment of mitotic spindle orientation Source: WormBase
  • regulation of mitotic cell cycle Source: WormBase

Keywordsi

Molecular functionDevelopmental protein

Enzyme and pathway databases

SignaLinkiO16519

Names & Taxonomyi

Protein namesi
Recommended name:
Gastrulation defective protein 1
Gene namesi
Name:gad-1
ORF Names:T05H4.14
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT05H4.14 ; CE13295 ; WBGene00001513 ; gad-1

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi324H → N in ct226; temperature-sensitive. In embryos from homozygous mutants, hermaphrodites reared at 25 degrees Celsius, the E cells divide early with abnormal spindle orientations and fail to migrate into the embryo, and no subsequent gastrulation movements occur. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003051411 – 620Gastrulation defective protein 1Add BLAST620

Proteomic databases

EPDiO16519
PaxDbiO16519
PeptideAtlasiO16519
PRIDEiO16519

PTM databases

iPTMnetiO16519

Expressioni

Developmental stagei

Expressed maternally. Present at high level in early embryos. In adult hermaphrodytes, expression is restricted to the germline.1 Publication

Gene expression databases

BgeeiWBGene00001513

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
nhr-111O455213EBI-327317,EBI-313236

Protein-protein interaction databases

BioGridi44073, 12 interactors
DIPiDIP-27096N
IntActiO16519, 15 interactors
STRINGi6239.T05H4.14.2

Structurei

3D structure databases

ProteinModelPortaliO16519
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati121 – 160WD 1Add BLAST40
Repeati168 – 207WD 2Add BLAST40
Repeati222 – 262WD 3Add BLAST41
Repeati278 – 317WD 4Add BLAST40
Repeati323 – 362WD 5Add BLAST40
Repeati415 – 455WD 6Add BLAST41

Sequence similaritiesi

Belongs to the WD repeat GAD-1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0772 Eukaryota
ENOG410XP76 LUCA
GeneTreeiENSGT00390000015433
HOGENOMiHOG000172867
InParanoidiO16519
OMAiATVRTWE
OrthoDBiEOG091G043W
PhylomeDBiO16519

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

O16519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDNTAPKKN SEKKARVFDL QAMFAQTISN APRTEEPTHV PQSVSVSSKS
60 70 80 90 100
ESGVSSSAVS DDEDDFMPDL PPGFQMQQSE AGPSSSRAEG PEDSDDSDFD
110 120 130 140 150
ETEAISIIKL IPAACEAKIS HGTQAISALR VEPPGVRFAS GGLDYYVKLF
160 170 180 190 200
DFQKMDMSMR YDKELLPAES HVINSLAFSP NGETLVVASG EAIIRLLDRA
210 220 230 240 250
GKQWSETVRG DQYIIDLNIT KGHTATVNCV EFNPLNKNEF ISCSDDGSLR
260 270 280 290 300
LWNLDDHKVI TKCINKHRKV IKTKGANGKR VSPQVCTFSP DGKWIAAGCD
310 320 330 340 350
DGSVQAWKYG SQYVNVNYLV RKAHNGSITS IAFSPDSKRL LSRGFDDTLK
360 370 380 390 400
MWSLDNPKEP LLVKTGLENA FKSTDCGFSP RAEVVFTGTS SPNKDTPGTL
410 420 430 440 450
QFFDPMTFDL VYKIDYPGIS CHRIQWHPRL NQIIAGLSDG TIHVYYDQTM
460 470 480 490 500
SQRGVMSCVT KPLKRNRASE VVREDMVLSP LSLEMFQPRG EEGEEKEVTG
510 520 530 540 550
WRIKKYLRMQ DNKLRPEFRK PADMPINGKS ANGRVAASGG SLHSYLAKQI
560 570 580 590 600
GTARNAEFLK DTDVRASILK HAKDAEENPL YIDKAYRKTQ PKKIFQETAV
610 620
EPEDQDDEEL QPVFKMPRTK
Length:620
Mass (Da):69,046
Last modified:January 1, 1998 - v1
Checksum:i2A928EB132A97387
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081501 Genomic DNA Translation: CCD72040.1
PIRiT31906
RefSeqiNP_504635.1, NM_072234.4
UniGeneiCel.8123

Genome annotation databases

EnsemblMetazoaiT05H4.14; T05H4.14; WBGene00001513
GeneIDi179027
KEGGicel:CELE_T05H4.14
UCSCiT05H4.14 c. elegans

Similar proteinsi

Entry informationi

Entry nameiGAD1_CAEEL
AccessioniPrimary (citable) accession number: O16519
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health