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Protein

Gastrulation defective protein 1

Gene

gad-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for initiation of gastrulation.1 Publication

GO - Biological processi

  • cell migration involved in gastrulation Source: WormBase
  • establishment of mitotic spindle orientation Source: WormBase
  • regulation of mitotic cell cycle Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

SignaLinkiO16519.

Names & Taxonomyi

Protein namesi
Recommended name:
Gastrulation defective protein 1
Gene namesi
Name:gad-1
ORF Names:T05H4.14
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT05H4.14; CE13295; WBGene00001513; gad-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi324 – 3241H → N in ct226; temperature-sensitive. In embryos from homozygous mutants, hermaphrodites reared at 25 degrees Celsius, the E cells divide early with abnormal spindle orientations and fail to migrate into the embryo, and no subsequent gastrulation movements occur. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 620620Gastrulation defective protein 1PRO_0000305141Add
BLAST

Proteomic databases

EPDiO16519.
PaxDbiO16519.

PTM databases

iPTMnetiO16519.

Expressioni

Developmental stagei

Expressed maternally. Present at high level in early embryos. In adult hermaphrodytes, expression is restricted to the germline.1 Publication

Gene expression databases

BgeeiWBGene00001513.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
nhr-111O455213EBI-327317,EBI-313236

Protein-protein interaction databases

BioGridi44073. 12 interactions.
DIPiDIP-27096N.
IntActiO16519. 13 interactions.
MINTiMINT-251309.
STRINGi6239.T05H4.14.2.

Structurei

3D structure databases

ProteinModelPortaliO16519.
SMRiO16519. Positions 123-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati121 – 16040WD 1Add
BLAST
Repeati168 – 20740WD 2Add
BLAST
Repeati222 – 26241WD 3Add
BLAST
Repeati278 – 31740WD 4Add
BLAST
Repeati323 – 36240WD 5Add
BLAST
Repeati415 – 45541WD 6Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat GAD-1 family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0772. Eukaryota.
ENOG410XP76. LUCA.
GeneTreeiENSGT00390000015433.
HOGENOMiHOG000172867.
InParanoidiO16519.
OMAiGCWHPKI.
OrthoDBiEOG091G043W.
PhylomeDBiO16519.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O16519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDNTAPKKN SEKKARVFDL QAMFAQTISN APRTEEPTHV PQSVSVSSKS
60 70 80 90 100
ESGVSSSAVS DDEDDFMPDL PPGFQMQQSE AGPSSSRAEG PEDSDDSDFD
110 120 130 140 150
ETEAISIIKL IPAACEAKIS HGTQAISALR VEPPGVRFAS GGLDYYVKLF
160 170 180 190 200
DFQKMDMSMR YDKELLPAES HVINSLAFSP NGETLVVASG EAIIRLLDRA
210 220 230 240 250
GKQWSETVRG DQYIIDLNIT KGHTATVNCV EFNPLNKNEF ISCSDDGSLR
260 270 280 290 300
LWNLDDHKVI TKCINKHRKV IKTKGANGKR VSPQVCTFSP DGKWIAAGCD
310 320 330 340 350
DGSVQAWKYG SQYVNVNYLV RKAHNGSITS IAFSPDSKRL LSRGFDDTLK
360 370 380 390 400
MWSLDNPKEP LLVKTGLENA FKSTDCGFSP RAEVVFTGTS SPNKDTPGTL
410 420 430 440 450
QFFDPMTFDL VYKIDYPGIS CHRIQWHPRL NQIIAGLSDG TIHVYYDQTM
460 470 480 490 500
SQRGVMSCVT KPLKRNRASE VVREDMVLSP LSLEMFQPRG EEGEEKEVTG
510 520 530 540 550
WRIKKYLRMQ DNKLRPEFRK PADMPINGKS ANGRVAASGG SLHSYLAKQI
560 570 580 590 600
GTARNAEFLK DTDVRASILK HAKDAEENPL YIDKAYRKTQ PKKIFQETAV
610 620
EPEDQDDEEL QPVFKMPRTK
Length:620
Mass (Da):69,046
Last modified:January 1, 1998 - v1
Checksum:i2A928EB132A97387
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081501 Genomic DNA. Translation: CCD72040.1.
PIRiT31906.
RefSeqiNP_504635.1. NM_072234.4.
UniGeneiCel.8123.

Genome annotation databases

EnsemblMetazoaiT05H4.14; T05H4.14; WBGene00001513.
GeneIDi179027.
KEGGicel:CELE_T05H4.14.
UCSCiT05H4.14. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081501 Genomic DNA. Translation: CCD72040.1.
PIRiT31906.
RefSeqiNP_504635.1. NM_072234.4.
UniGeneiCel.8123.

3D structure databases

ProteinModelPortaliO16519.
SMRiO16519. Positions 123-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44073. 12 interactions.
DIPiDIP-27096N.
IntActiO16519. 13 interactions.
MINTiMINT-251309.
STRINGi6239.T05H4.14.2.

PTM databases

iPTMnetiO16519.

Proteomic databases

EPDiO16519.
PaxDbiO16519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05H4.14; T05H4.14; WBGene00001513.
GeneIDi179027.
KEGGicel:CELE_T05H4.14.
UCSCiT05H4.14. c. elegans.

Organism-specific databases

CTDi179027.
WormBaseiT05H4.14; CE13295; WBGene00001513; gad-1.

Phylogenomic databases

eggNOGiKOG0772. Eukaryota.
ENOG410XP76. LUCA.
GeneTreeiENSGT00390000015433.
HOGENOMiHOG000172867.
InParanoidiO16519.
OMAiGCWHPKI.
OrthoDBiEOG091G043W.
PhylomeDBiO16519.

Enzyme and pathway databases

SignaLinkiO16519.

Miscellaneous databases

PROiO16519.

Gene expression databases

BgeeiWBGene00001513.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAD1_CAEEL
AccessioniPrimary (citable) accession number: O16519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.