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O16299

- FIGL1_CAEEL

UniProt

O16299 - FIGL1_CAEEL

Protein

Fidgetin-like protein 1

Gene

figl-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Has a role in spindle assembly which acts in the progression through mitosis during embryogenesis. Required for fertility.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.1 Publication

    Cofactori

    Magnesium.1 Publication

    Kineticsi

    At 25 degrees Celsius and pH 8.0.

    1. KM=0.44 mM for ATP1 Publication

    Vmax=225 nmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 8.0-8.8.1 Publication

    Temperature dependencei

    Optimum temperature is 25-30 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei319 – 3191ATP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi359 – 3646ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity Source: WormBase
    2. ATP binding Source: UniProtKB-KW
    3. hydrolase activity Source: UniProtKB
    4. magnesium ion binding Source: UniProtKB
    5. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: WormBase
    2. ATP metabolic process Source: UniProtKB
    3. mitotic nuclear division Source: UniProtKB-KW
    4. positive regulation of mitotic cell cycle Source: WormBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKO16299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fidgetin-like protein 1 (EC:3.6.4.-)
    Alternative name(s):
    Fidgetin homolog
    Gene namesi
    Name:figl-1
    ORF Names:F32D1.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome V

    Organism-specific databases

    WormBaseiF32D1.1; CE09865; WBGene00017981; figl-1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: WormBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Sterility owing to depletion of germ cells.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi360 – 3601T → C: No effect on ATPase activity. 1 Publication
    Mutagenesisi362 – 3621K → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi368 – 3681C → A: Strongly inhibits ATPase activity. 1 Publication
    Mutagenesisi373 – 3731C → A: Slightly inhibits ATPase activity. 1 Publication
    Mutagenesisi416 – 4161E → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi461 – 4611N → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi471 – 4711R → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi472 – 4721R → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi473 – 4731R → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi527 – 5271C → A: Slightly inhibits ATPase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 594594Fidgetin-like protein 1PRO_0000302729Add
    BLAST

    Proteomic databases

    PaxDbiO16299.
    PRIDEiO16299.

    Expressioni

    Tissue specificityi

    Expressed in germ cells.1 Publication

    Interactioni

    Subunit structurei

    Hexamer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gei-18Q7K7J04EBI-320880,EBI-2315822
    itsn-1Q9U2T93EBI-320880,EBI-2414252
    sorb-1O624826EBI-320880,EBI-325337
    T04C9.1Q8MPT25EBI-320880,EBI-2315635

    Protein-protein interaction databases

    BioGridi43882. 9 interactions.
    DIPiDIP-25869N.
    IntActiO16299. 18 interactions.
    MINTiMINT-117972.
    STRINGi6239.F32D1.1.

    Structurei

    Secondary structure

    1
    594
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi289 – 2913
    Helixi296 – 30510
    Turni314 – 3174
    Helixi322 – 33110
    Helixi333 – 3375
    Turni339 – 3413
    Helixi344 – 3463
    Beta strandi350 – 36011
    Helixi362 – 37211
    Beta strandi376 – 3816
    Helixi382 – 3865
    Turni387 – 3893
    Helixi393 – 40614
    Beta strandi407 – 4159
    Helixi417 – 4215
    Helixi430 – 44617
    Beta strandi454 – 4618
    Helixi463 – 4653
    Turni468 – 4736
    Beta strandi475 – 4795
    Helixi485 – 49612
    Helixi505 – 51410
    Helixi520 – 53617
    Helixi537 – 5437
    Helixi547 – 5493
    Helixi555 – 56410
    Helixi571 – 58414

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4L15X-ray2.60A261-594[»]
    4L16X-ray2.80A261-594[»]
    ProteinModelPortaliO16299.
    SMRiO16299. Positions 286-588.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00570000078874.
    HOGENOMiHOG000112588.
    InParanoidiO16299.
    OMAiHFDENII.
    OrthoDBiEOG7GXPCR.
    PhylomeDBiO16299.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O16299-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYSPKRVKLN VTSGMRKRPE TGENNDDLYP PTALARNGIS PYFIGKPRRK    50
    IVVETPSDSA QQQPPFKSRS QQNGLDDELD GIIIDEDEDR TVDVSFSQKQ 100
    DTRKLKSRPF LGEKSSFKLG EIPKPKEEKR REEPFTMRGF DFGSDDKVTK 150
    IRDKICDIVD PTNARRTDPN FIRQMHENTL KGIEVASNPH FKKTRAPTKN 200
    RAAIQNTLGT LYPSFTTAAG QDPQNSKFQV PLDRQSSSQS IGSLAGIPPA 250
    RRAPDIPKRC SNPLIRKAMG MDTEGGGKDE KMSGLRAEPT LKHFDENIIS 300
    LIESEIMSVN NEIGWADVAG LEGAKKALRE IVVLPFKRPD VFTGIRAPPK 350
    GVLLFGPPGT GKTMIGRCVA SQCKATFFNI SASSLTSKWV GEGEKLVRAL 400
    FSVARLKLPS VIFIDEIDSL LSSRSESEHE SSRRIKTEFL VQLDGVNTAP 450
    DERLLVLGAT NRPQELDEAA RRRFQKRLYI ALPEPESRTQ IVQNLLVGTR 500
    HDITNHNLER IRELTDGYSG ADMRQLCTEA AMGPIRDIGD DIETIDKDDI 550
    RAVTVMDFAE AARVVRPTVD DSQLDAYAAW DKKFGCLPPP SISR 594
    Length:594
    Mass (Da):66,187
    Last modified:January 1, 1998 - v1
    Checksum:i59E0FFBA05B6712F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO081012 Genomic DNA. Translation: CCD68481.1.
    PIRiT03922.
    RefSeqiNP_504197.1. NM_071796.3.
    UniGeneiCel.6491.

    Genome annotation databases

    EnsemblMetazoaiF32D1.1; F32D1.1; WBGene00017981.
    GeneIDi178829.
    KEGGicel:CELE_F32D1.1.
    UCSCiF32D1.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO081012 Genomic DNA. Translation: CCD68481.1 .
    PIRi T03922.
    RefSeqi NP_504197.1. NM_071796.3.
    UniGenei Cel.6491.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4L15 X-ray 2.60 A 261-594 [» ]
    4L16 X-ray 2.80 A 261-594 [» ]
    ProteinModelPortali O16299.
    SMRi O16299. Positions 286-588.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 43882. 9 interactions.
    DIPi DIP-25869N.
    IntActi O16299. 18 interactions.
    MINTi MINT-117972.
    STRINGi 6239.F32D1.1.

    Proteomic databases

    PaxDbi O16299.
    PRIDEi O16299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F32D1.1 ; F32D1.1 ; WBGene00017981 .
    GeneIDi 178829.
    KEGGi cel:CELE_F32D1.1.
    UCSCi F32D1.1. c. elegans.

    Organism-specific databases

    CTDi 178829.
    WormBasei F32D1.1 ; CE09865 ; WBGene00017981 ; figl-1.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00570000078874.
    HOGENOMi HOG000112588.
    InParanoidi O16299.
    OMAi HFDENII.
    OrthoDBi EOG7GXPCR.
    PhylomeDBi O16299.

    Enzyme and pathway databases

    SABIO-RK O16299.

    Miscellaneous databases

    NextBioi 902732.
    PROi O16299.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Identification of a cysteine residue important for the ATPase activity of C. elegans fidgetin homologue."
      Yakushiji Y., Yamanaka K., Ogura T.
      FEBS Lett. 578:191-197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-360; CYS-368; CYS-373 AND CYS-527.
    3. "Mutational analysis of the functional motifs in the ATPase domain of Caenorhabditis elegans fidgetin homologue FIGL-1: firm evidence for an intersubunit catalysis mechanism of ATP hydrolysis by AAA ATPases."
      Yakushiji Y., Nishikori S., Yamanaka K., Ogura T.
      J. Struct. Biol. 156:93-100(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, MUTAGENESIS OF LYS-362; GLU-416; ASN-461; ARG-471; ARG-472 AND ARG-473.
    4. "The AAA-ATPase FIGL-1 controls mitotic progression, and its levels are regulated by the CUL-3MEL-26 E3 ligase in the C. elegans germ line."
      Luke-Glaser S., Pintard L., Tyers M., Peter M.
      J. Cell Sci. 120:3179-3187(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiFIGL1_CAEEL
    AccessioniPrimary (citable) accession number: O16299
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3