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Protein

Fidgetin-like protein 1

Gene

figl-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in spindle assembly which acts in the progression through mitosis during embryogenesis. Required for fertility.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

At 25 degrees Celsius and pH 8.0.

  1. KM=0.44 mM for ATP1 Publication
  1. Vmax=225 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0-8.8.1 Publication

Temperature dependencei

Optimum temperature is 25-30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei319ATP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi359 – 364ATPBy similarity6

GO - Molecular functioni

  • ATPase activity Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • hydrolase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • microtubule binding Source: WormBase
  • microtubule-severing ATPase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKO16299.

Names & Taxonomyi

Protein namesi
Recommended name:
Fidgetin-like protein 1 (EC:3.6.4.-)
Alternative name(s):
Fidgetin homolog
Gene namesi
Name:figl-1
ORF Names:F32D1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF32D1.1; CE09865; WBGene00017981; figl-1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Sterility owing to depletion of germ cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi360T → C: No effect on ATPase activity. 1 Publication1
Mutagenesisi362K → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi368C → A: Strongly inhibits ATPase activity. 1 Publication1
Mutagenesisi373C → A: Slightly inhibits ATPase activity. 1 Publication1
Mutagenesisi416E → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi461N → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi471R → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi472R → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi473R → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi527C → A: Slightly inhibits ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003027291 – 594Fidgetin-like protein 1Add BLAST594

Proteomic databases

EPDiO16299.
PaxDbiO16299.
PeptideAtlasiO16299.
PRIDEiO16299.

Expressioni

Tissue specificityi

Expressed in germ cells.1 Publication

Gene expression databases

BgeeiWBGene00017981.

Interactioni

Subunit structurei

Hexamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
gei-18Q7K7J04EBI-320880,EBI-2315822
itsn-1Q9U2T93EBI-320880,EBI-2414252
sorb-1O624826EBI-320880,EBI-325337
T04C9.1Q8MPT25EBI-320880,EBI-2315635

GO - Molecular functioni

  • microtubule binding Source: WormBase

Protein-protein interaction databases

BioGridi43882. 9 interactors.
DIPiDIP-25869N.
IntActiO16299. 19 interactors.
MINTiMINT-117972.
STRINGi6239.F32D1.1.

Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi289 – 291Combined sources3
Helixi296 – 305Combined sources10
Turni314 – 317Combined sources4
Helixi322 – 331Combined sources10
Helixi333 – 337Combined sources5
Turni339 – 341Combined sources3
Helixi344 – 346Combined sources3
Beta strandi350 – 360Combined sources11
Helixi362 – 372Combined sources11
Beta strandi376 – 381Combined sources6
Helixi382 – 386Combined sources5
Turni387 – 389Combined sources3
Helixi393 – 406Combined sources14
Beta strandi407 – 415Combined sources9
Helixi417 – 421Combined sources5
Helixi430 – 446Combined sources17
Beta strandi454 – 461Combined sources8
Helixi463 – 465Combined sources3
Turni468 – 473Combined sources6
Beta strandi475 – 479Combined sources5
Helixi485 – 496Combined sources12
Helixi505 – 514Combined sources10
Helixi520 – 536Combined sources17
Helixi537 – 543Combined sources7
Helixi547 – 549Combined sources3
Helixi555 – 564Combined sources10
Helixi571 – 584Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L15X-ray2.60A261-594[»]
4L16X-ray2.80A261-594[»]
ProteinModelPortaliO16299.
SMRiO16299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0740. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00570000078874.
HOGENOMiHOG000112588.
InParanoidiO16299.
OMAiHFDENII.
OrthoDBiEOG091G0Q8J.
PhylomeDBiO16299.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O16299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSPKRVKLN VTSGMRKRPE TGENNDDLYP PTALARNGIS PYFIGKPRRK
60 70 80 90 100
IVVETPSDSA QQQPPFKSRS QQNGLDDELD GIIIDEDEDR TVDVSFSQKQ
110 120 130 140 150
DTRKLKSRPF LGEKSSFKLG EIPKPKEEKR REEPFTMRGF DFGSDDKVTK
160 170 180 190 200
IRDKICDIVD PTNARRTDPN FIRQMHENTL KGIEVASNPH FKKTRAPTKN
210 220 230 240 250
RAAIQNTLGT LYPSFTTAAG QDPQNSKFQV PLDRQSSSQS IGSLAGIPPA
260 270 280 290 300
RRAPDIPKRC SNPLIRKAMG MDTEGGGKDE KMSGLRAEPT LKHFDENIIS
310 320 330 340 350
LIESEIMSVN NEIGWADVAG LEGAKKALRE IVVLPFKRPD VFTGIRAPPK
360 370 380 390 400
GVLLFGPPGT GKTMIGRCVA SQCKATFFNI SASSLTSKWV GEGEKLVRAL
410 420 430 440 450
FSVARLKLPS VIFIDEIDSL LSSRSESEHE SSRRIKTEFL VQLDGVNTAP
460 470 480 490 500
DERLLVLGAT NRPQELDEAA RRRFQKRLYI ALPEPESRTQ IVQNLLVGTR
510 520 530 540 550
HDITNHNLER IRELTDGYSG ADMRQLCTEA AMGPIRDIGD DIETIDKDDI
560 570 580 590
RAVTVMDFAE AARVVRPTVD DSQLDAYAAW DKKFGCLPPP SISR
Length:594
Mass (Da):66,187
Last modified:January 1, 1998 - v1
Checksum:i59E0FFBA05B6712F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68481.1.
PIRiT03922.
RefSeqiNP_504197.1. NM_071796.3.
UniGeneiCel.6491.

Genome annotation databases

EnsemblMetazoaiF32D1.1; F32D1.1; WBGene00017981.
GeneIDi178829.
KEGGicel:CELE_F32D1.1.
UCSCiF32D1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68481.1.
PIRiT03922.
RefSeqiNP_504197.1. NM_071796.3.
UniGeneiCel.6491.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4L15X-ray2.60A261-594[»]
4L16X-ray2.80A261-594[»]
ProteinModelPortaliO16299.
SMRiO16299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43882. 9 interactors.
DIPiDIP-25869N.
IntActiO16299. 19 interactors.
MINTiMINT-117972.
STRINGi6239.F32D1.1.

Proteomic databases

EPDiO16299.
PaxDbiO16299.
PeptideAtlasiO16299.
PRIDEiO16299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF32D1.1; F32D1.1; WBGene00017981.
GeneIDi178829.
KEGGicel:CELE_F32D1.1.
UCSCiF32D1.1. c. elegans.

Organism-specific databases

CTDi178829.
WormBaseiF32D1.1; CE09865; WBGene00017981; figl-1.

Phylogenomic databases

eggNOGiKOG0740. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00570000078874.
HOGENOMiHOG000112588.
InParanoidiO16299.
OMAiHFDENII.
OrthoDBiEOG091G0Q8J.
PhylomeDBiO16299.

Enzyme and pathway databases

SABIO-RKO16299.

Miscellaneous databases

PROiO16299.

Gene expression databases

BgeeiWBGene00017981.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIGL1_CAEEL
AccessioniPrimary (citable) accession number: O16299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.