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O16299

- FIGL1_CAEEL

UniProt

O16299 - FIGL1_CAEEL

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Protein

Fidgetin-like protein 1

Gene

figl-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in spindle assembly which acts in the progression through mitosis during embryogenesis. Required for fertility.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

At 25 degrees Celsius and pH 8.0.

  1. KM=0.44 mM for ATP1 Publication

Vmax=225 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0-8.8.1 Publication

Temperature dependencei

Optimum temperature is 25-30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei319 – 3191ATP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi359 – 3646ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: WormBase
  2. ATP binding Source: UniProtKB-KW
  3. hydrolase activity Source: UniProtKB
  4. magnesium ion binding Source: UniProtKB
  5. microtubule binding Source: WormBase

GO - Biological processi

  1. ATP catabolic process Source: WormBase
  2. ATP metabolic process Source: UniProtKB
  3. mitotic nuclear division Source: UniProtKB-KW
  4. positive regulation of mitotic cell cycle Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKO16299.

Names & Taxonomyi

Protein namesi
Recommended name:
Fidgetin-like protein 1 (EC:3.6.4.-)
Alternative name(s):
Fidgetin homolog
Gene namesi
Name:figl-1
ORF Names:F32D1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome V

Organism-specific databases

WormBaseiF32D1.1; CE09865; WBGene00017981; figl-1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Sterility owing to depletion of germ cells.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi360 – 3601T → C: No effect on ATPase activity. 1 Publication
Mutagenesisi362 – 3621K → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi368 – 3681C → A: Strongly inhibits ATPase activity. 1 Publication
Mutagenesisi373 – 3731C → A: Slightly inhibits ATPase activity. 1 Publication
Mutagenesisi416 – 4161E → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi461 – 4611N → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi471 – 4711R → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi472 – 4721R → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi473 – 4731R → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi527 – 5271C → A: Slightly inhibits ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 594594Fidgetin-like protein 1PRO_0000302729Add
BLAST

Proteomic databases

PaxDbiO16299.
PRIDEiO16299.

Expressioni

Tissue specificityi

Expressed in germ cells.1 Publication

Interactioni

Subunit structurei

Hexamer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
gei-18Q7K7J04EBI-320880,EBI-2315822
itsn-1Q9U2T93EBI-320880,EBI-2414252
sorb-1O624826EBI-320880,EBI-325337
T04C9.1Q8MPT25EBI-320880,EBI-2315635

Protein-protein interaction databases

BioGridi43882. 9 interactions.
DIPiDIP-25869N.
IntActiO16299. 18 interactions.
MINTiMINT-117972.
STRINGi6239.F32D1.1.

Structurei

Secondary structure

1
594
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi289 – 2913Combined sources
Helixi296 – 30510Combined sources
Turni314 – 3174Combined sources
Helixi322 – 33110Combined sources
Helixi333 – 3375Combined sources
Turni339 – 3413Combined sources
Helixi344 – 3463Combined sources
Beta strandi350 – 36011Combined sources
Helixi362 – 37211Combined sources
Beta strandi376 – 3816Combined sources
Helixi382 – 3865Combined sources
Turni387 – 3893Combined sources
Helixi393 – 40614Combined sources
Beta strandi407 – 4159Combined sources
Helixi417 – 4215Combined sources
Helixi430 – 44617Combined sources
Beta strandi454 – 4618Combined sources
Helixi463 – 4653Combined sources
Turni468 – 4736Combined sources
Beta strandi475 – 4795Combined sources
Helixi485 – 49612Combined sources
Helixi505 – 51410Combined sources
Helixi520 – 53617Combined sources
Helixi537 – 5437Combined sources
Helixi547 – 5493Combined sources
Helixi555 – 56410Combined sources
Helixi571 – 58414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L15X-ray2.60A261-594[»]
4L16X-ray2.80A261-594[»]
ProteinModelPortaliO16299.
SMRiO16299. Positions 286-588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00570000078874.
HOGENOMiHOG000112588.
InParanoidiO16299.
OMAiHFDENII.
OrthoDBiEOG7GXPCR.
PhylomeDBiO16299.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O16299-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYSPKRVKLN VTSGMRKRPE TGENNDDLYP PTALARNGIS PYFIGKPRRK
60 70 80 90 100
IVVETPSDSA QQQPPFKSRS QQNGLDDELD GIIIDEDEDR TVDVSFSQKQ
110 120 130 140 150
DTRKLKSRPF LGEKSSFKLG EIPKPKEEKR REEPFTMRGF DFGSDDKVTK
160 170 180 190 200
IRDKICDIVD PTNARRTDPN FIRQMHENTL KGIEVASNPH FKKTRAPTKN
210 220 230 240 250
RAAIQNTLGT LYPSFTTAAG QDPQNSKFQV PLDRQSSSQS IGSLAGIPPA
260 270 280 290 300
RRAPDIPKRC SNPLIRKAMG MDTEGGGKDE KMSGLRAEPT LKHFDENIIS
310 320 330 340 350
LIESEIMSVN NEIGWADVAG LEGAKKALRE IVVLPFKRPD VFTGIRAPPK
360 370 380 390 400
GVLLFGPPGT GKTMIGRCVA SQCKATFFNI SASSLTSKWV GEGEKLVRAL
410 420 430 440 450
FSVARLKLPS VIFIDEIDSL LSSRSESEHE SSRRIKTEFL VQLDGVNTAP
460 470 480 490 500
DERLLVLGAT NRPQELDEAA RRRFQKRLYI ALPEPESRTQ IVQNLLVGTR
510 520 530 540 550
HDITNHNLER IRELTDGYSG ADMRQLCTEA AMGPIRDIGD DIETIDKDDI
560 570 580 590
RAVTVMDFAE AARVVRPTVD DSQLDAYAAW DKKFGCLPPP SISR
Length:594
Mass (Da):66,187
Last modified:January 1, 1998 - v1
Checksum:i59E0FFBA05B6712F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68481.1.
PIRiT03922.
RefSeqiNP_504197.1. NM_071796.3.
UniGeneiCel.6491.

Genome annotation databases

EnsemblMetazoaiF32D1.1; F32D1.1; WBGene00017981.
GeneIDi178829.
KEGGicel:CELE_F32D1.1.
UCSCiF32D1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68481.1 .
PIRi T03922.
RefSeqi NP_504197.1. NM_071796.3.
UniGenei Cel.6491.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4L15 X-ray 2.60 A 261-594 [» ]
4L16 X-ray 2.80 A 261-594 [» ]
ProteinModelPortali O16299.
SMRi O16299. Positions 286-588.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 43882. 9 interactions.
DIPi DIP-25869N.
IntActi O16299. 18 interactions.
MINTi MINT-117972.
STRINGi 6239.F32D1.1.

Proteomic databases

PaxDbi O16299.
PRIDEi O16299.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F32D1.1 ; F32D1.1 ; WBGene00017981 .
GeneIDi 178829.
KEGGi cel:CELE_F32D1.1.
UCSCi F32D1.1. c. elegans.

Organism-specific databases

CTDi 178829.
WormBasei F32D1.1 ; CE09865 ; WBGene00017981 ; figl-1.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00570000078874.
HOGENOMi HOG000112588.
InParanoidi O16299.
OMAi HFDENII.
OrthoDBi EOG7GXPCR.
PhylomeDBi O16299.

Enzyme and pathway databases

SABIO-RK O16299.

Miscellaneous databases

NextBioi 902732.
PROi O16299.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Identification of a cysteine residue important for the ATPase activity of C. elegans fidgetin homologue."
    Yakushiji Y., Yamanaka K., Ogura T.
    FEBS Lett. 578:191-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF THR-360; CYS-368; CYS-373 AND CYS-527.
  3. "Mutational analysis of the functional motifs in the ATPase domain of Caenorhabditis elegans fidgetin homologue FIGL-1: firm evidence for an intersubunit catalysis mechanism of ATP hydrolysis by AAA ATPases."
    Yakushiji Y., Nishikori S., Yamanaka K., Ogura T.
    J. Struct. Biol. 156:93-100(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, MUTAGENESIS OF LYS-362; GLU-416; ASN-461; ARG-471; ARG-472 AND ARG-473.
  4. "The AAA-ATPase FIGL-1 controls mitotic progression, and its levels are regulated by the CUL-3MEL-26 E3 ligase in the C. elegans germ line."
    Luke-Glaser S., Pintard L., Tyers M., Peter M.
    J. Cell Sci. 120:3179-3187(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiFIGL1_CAEEL
AccessioniPrimary (citable) accession number: O16299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3