Reviewed, UniProtKB/Swiss-Prot O16294 (GMPR_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

ORF Names:

F32D1.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.
Sequence similarities	Belongs to the IMPDH/GMPR family.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase nucleotide metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Probable GMP reductase		PRO_0000093729
Regions
	Nucleotide binding	109 – 132	24	NADP By similarity
Sites
	Active site	187	1	Thioimidate intermediate By similarity
	Metal binding	182	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	184	1	Potassium; via carbonyl oxygen By similarity
	Binding site	220	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O16294-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A4AEE84F2CB72360	FASTA	358	38,806
	10 20 30 40 50 60 MPRIENEPKL DFKDVLLRPK RSTLKSRADV ELDREYVFRN SKATYTGVPV VASNMDTVGT 70 80 90 100 110 120 FEMAAALNNH KIFTTIHKHY SVDEWKAFAA SASPDTFNNL AISSGISDND WTKLNTVITE 130 140 150 160 170 180 LPQLKYICLD VANGYSESFV EFIRRVREAY PKHTIMAGNV VTGEMVEELI LSGADIVKVG 190 200 210 220 230 240 IGPGSVCTTR KKAGVGYPQL SAVLECADAA HGLNGHVMSD GGCSNPGDVA KAFGAGADFV 250 260 270 280 290 300 MIGGLFAGHD QSGGDLIEHN GKKFKLFYGM SSDTAMKKHH GSVAEYRASE GKTVTIPYRG 310 320 330 340 350 DVNGTVQDIL GGIRSACTYT GAKHLKELAK RATFIRVTQQ TNDMYVPFEV PTVPAPSK

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF016427 Genomic DNA. Translation: AAB65350.1.
PIR	T03917.
RefSeq	NP_504202.1.
UniGene	Cel.6523
3D structure databases
HSSP	HSSP built from PDB template 1EEP based on UniProtKB P49058.
SMR	O16294. Positions 2-339.
ModBase	Search...
Genome annotation databases
Ensembl	F32D1.5. Caenorhabditis elegans. [Contig view]
GeneID	178834.
KEGG	cel:F32D1.5.
Organism-specific databases
WormBase	WBGene00017984. F32D1.5.
WormPep	F32D1.5. CE09869. [WorfDB]
Phylogenomic databases
OMA	O16294. NSRSECD.
Enzyme and pathway databases
BRENDA	1.7.1.7. 672.
Gene expression databases
ArrayExpress	O16294.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	902748.

Entry information

Entry name

GMPR_CAEEL

Accession

Primary (citable) accession number: O16294

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project