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Protein

GMP reductase

Gene

F32D1.5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781NADPUniRule annotation
Metal bindingi182 – 1821Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi184 – 1841Potassium; via carbonyl oxygenUniRule annotation
Active sitei187 – 1871Thioimidate intermediateUniRule annotation
Metal bindingi187 – 1871Potassium; via carbonyl oxygenUniRule annotation
Active sitei189 – 1891Proton donor/acceptorUniRule annotation
Metal bindingi190 – 1901PotassiumUniRule annotation
Binding sitei270 – 2701NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 272NADP; shared with neighboring subunitUniRule annotation
Nucleotide bindingi130 – 1323NADPUniRule annotation
Nucleotide bindingi181 – 1822NADPUniRule annotation
Nucleotide bindingi286 – 2872NADPUniRule annotation
Nucleotide bindingi315 – 3184NADP; shared with neighboring subunitUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Metal-binding, NADP, Potassium

Enzyme and pathway databases

ReactomeiR-CEL-74217. Purine salvage.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP reductaseUniRule annotation (EC:1.7.1.7UniRule annotation)
Short name:
GMPRUniRule annotation
Alternative name(s):
Guanosine 5'-monophosphate oxidoreductaseUniRule annotation
Short name:
Guanosine monophosphate reductaseUniRule annotation
Gene namesi
ORF Names:F32D1.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF32D1.5; CE09869; WBGene00017984.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358GMP reductasePRO_0000093729Add
BLAST

Proteomic databases

EPDiO16294.
PaxDbiO16294.
PRIDEiO16294.

Expressioni

Gene expression databases

BgeeiWBGene00017984.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi43886. 1 interaction.
STRINGi6239.F32D1.5.1.

Structurei

3D structure databases

ProteinModelPortaliO16294.
SMRiO16294. Positions 10-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni220 – 2223GMP bindingUniRule annotation
Regioni243 – 2442GMP bindingUniRule annotation
Regioni269 – 2713GMP bindingUniRule annotation
Regioni287 – 2915GMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2550. Eukaryota.
COG0516. LUCA.
GeneTreeiENSGT00530000062923.
HOGENOMiHOG000165756.
InParanoidiO16294.
KOiK00364.
OMAiCSCAGDV.
OrthoDBiEOG091G09KQ.
PhylomeDBiO16294.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00596. GMP_reduct_type1. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005993. GMPR.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsiTIGR01305. GMP_reduct_1. 1 hit.
PROSITEiPS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O16294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRIENEPKL DFKDVLLRPK RSTLKSRADV ELDREYVFRN SKATYTGVPV
60 70 80 90 100
VASNMDTVGT FEMAAALNNH KIFTTIHKHY SVDEWKAFAA SASPDTFNNL
110 120 130 140 150
AISSGISDND WTKLNTVITE LPQLKYICLD VANGYSESFV EFIRRVREAY
160 170 180 190 200
PKHTIMAGNV VTGEMVEELI LSGADIVKVG IGPGSVCTTR KKAGVGYPQL
210 220 230 240 250
SAVLECADAA HGLNGHVMSD GGCSNPGDVA KAFGAGADFV MIGGLFAGHD
260 270 280 290 300
QSGGDLIEHN GKKFKLFYGM SSDTAMKKHH GSVAEYRASE GKTVTIPYRG
310 320 330 340 350
DVNGTVQDIL GGIRSACTYT GAKHLKELAK RATFIRVTQQ TNDMYVPFEV

PTVPAPSK
Length:358
Mass (Da):38,806
Last modified:January 1, 1998 - v1
Checksum:iA4AEE84F2CB72360
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68485.1.
PIRiT03917.
RefSeqiNP_504202.1. NM_071801.6.
UniGeneiCel.6523.

Genome annotation databases

EnsemblMetazoaiF32D1.5; F32D1.5; WBGene00017984.
GeneIDi178834.
KEGGicel:CELE_F32D1.5.
UCSCiF32D1.5.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081012 Genomic DNA. Translation: CCD68485.1.
PIRiT03917.
RefSeqiNP_504202.1. NM_071801.6.
UniGeneiCel.6523.

3D structure databases

ProteinModelPortaliO16294.
SMRiO16294. Positions 10-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43886. 1 interaction.
STRINGi6239.F32D1.5.1.

Proteomic databases

EPDiO16294.
PaxDbiO16294.
PRIDEiO16294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF32D1.5; F32D1.5; WBGene00017984.
GeneIDi178834.
KEGGicel:CELE_F32D1.5.
UCSCiF32D1.5.1. c. elegans.

Organism-specific databases

CTDi178834.
WormBaseiF32D1.5; CE09869; WBGene00017984.

Phylogenomic databases

eggNOGiKOG2550. Eukaryota.
COG0516. LUCA.
GeneTreeiENSGT00530000062923.
HOGENOMiHOG000165756.
InParanoidiO16294.
KOiK00364.
OMAiCSCAGDV.
OrthoDBiEOG091G09KQ.
PhylomeDBiO16294.

Enzyme and pathway databases

ReactomeiR-CEL-74217. Purine salvage.

Miscellaneous databases

PROiO16294.

Gene expression databases

BgeeiWBGene00017984.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00596. GMP_reduct_type1. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005993. GMPR.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsiTIGR01305. GMP_reduct_1. 1 hit.
PROSITEiPS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMPR_CAEEL
AccessioniPrimary (citable) accession number: O16294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.