Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione-independent glyoxalase DJR-1.2

Gene

djr-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) or glyoxal (GO) to D-lactate or glycolic acid respectively in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against glyoxal-induced cell death. Protects dopaminergic neurons from glyoxal-dependent neuronal degeneration.2 Publications

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 60.0 min(-1) with methylglyoxal as substrate and 146.4 min(-1) with glyoxal as substrate.1 Publication
  1. KM=0.39 mM for methylglyoxal1 Publication
  2. KM=0.78 mM for glyoxal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei20By similarity1
    Active sitei105By similarity1
    Active sitei124By similarity1

    GO - Molecular functioni

    GO - Biological processi

    • cellular response to glyoxal Source: ParkinsonsUK-UCL
    • cellular response to methylglyoxal Source: ParkinsonsUK-UCL
    • glycolate biosynthetic process Source: ParkinsonsUK-UCL
    • glyoxal metabolic process Source: ParkinsonsUK-UCL
    • lactate biosynthetic process Source: ParkinsonsUK-UCL
    • methylglyoxal metabolic process Source: ParkinsonsUK-UCL
    • negative regulation of neuron death Source: ParkinsonsUK-UCL

    Keywordsi

    Molecular functionLyase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase DJR-1.21 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Protein DJ-1 homolog 2
    Gene namesi
    Name:djr-1.2Imported
    ORF Names:C49G7.11
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    Proteomesi
    • UP000001940 Componenti: Chromosome V

    Organism-specific databases

    WormBaseiC49G7.11 ; CE08869 ; WBGene00016789 ; djr-1.2

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004321091 – 186Glutathione-independent glyoxalase DJR-1.2Add BLAST186

    Proteomic databases

    PaxDbiO16228
    PeptideAtlasiO16228

    Expressioni

    Tissue specificityi

    Expressed in various tissues, including pharyngeal muscles, pharynx-intestinal valve, ventral nerve cord, spermatheca, rectal gland, inner labial (IL) cells of head neurons, phasmid (PHA/PHB) neurons in tail and supporting sheath/socket cells, as well as in head mesodermal cells (HMC), excretory canals and coelomocytes.1 Publication

    Inductioni

    Induced by DAF-16 during starvation as well as in the dauer stage.1 Publication

    Gene expression databases

    BgeeiWBGene00016789

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.C49G7.11

    Structurei

    3D structure databases

    ProteinModelPortaliO16228
    SMRiO16228
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family. DJ-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG2764 Eukaryota
    COG0693 LUCA
    GeneTreeiENSGT00390000001231
    HOGENOMiHOG000063194
    InParanoidiO16228
    KOiK05687
    OMAiEACAGKS
    OrthoDBiEOG091G12NS
    PhylomeDBiO16228

    Family and domain databases

    Gene3Di3.40.50.880, 1 hit
    InterProiView protein in InterPro
    IPR029062 Class_I_gatase-like
    IPR006287 DJ-1
    IPR002818 DJ-1/PfpI
    PfamiView protein in Pfam
    PF01965 DJ-1_PfpI, 1 hit
    SUPFAMiSSF52317 SSF52317, 1 hit
    TIGRFAMsiTIGR01383 not_thiJ, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O16228-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAAQKSALIL LPPEDAEEIE VIVTGDVLVR GGLQVLYAGS STEPVKCAKG
    60 70 80 90 100
    ARIVPDVALK DVKNKTFDII IIPGGPGCSK LAECPVIGEL LKTQVKSGGL
    110 120 130 140 150
    IGAICAGPTV LLAHGIVAER VTCHYTVKDK MTEGGYKYLD DNVVISDRVI
    160 170 180
    TSKGPGTAFE FALKIVETLE GPEKTNSLLK PLCLAK
    Length:186
    Mass (Da):19,556
    Last modified:January 1, 1998 - v1
    Checksum:iDE31E1AF30C011E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO080906 Genomic DNA Translation: CCD67692.1
    PIRiT03871
    RefSeqiNP_504132.1, NM_071731.1
    UniGeneiCel.4466

    Genome annotation databases

    EnsemblMetazoaiC49G7.11; C49G7.11; WBGene00016789
    GeneIDi183625
    KEGGicel:CELE_C49G7.11
    UCSCiC49G7.11 c. elegans

    Similar proteinsi

    Entry informationi

    Entry nameiDJ12_CAEEL
    AccessioniPrimary (citable) accession number: O16228
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: January 1, 1998
    Last modified: May 23, 2018
    This is version 108 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health