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Protein

Glutathione-independent glyoxalase DJR-1.2

Gene

djr-1.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) or glyoxal (GO) to D-lactate or glycolic acid respectively in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals. Involved in protection against glyoxal-induced cell death. Protects dopaminergic neurons from glyoxal-dependent neuronal degeneration.2 Publications

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 60.0 min(-1) with methylglyoxal as substrate and 146.4 min(-1) with glyoxal as substrate.1 Publication

  1. KM=0.39 mM for methylglyoxal1 Publication
  2. KM=0.78 mM for glyoxal1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei20 – 201By similarity
    Active sitei105 – 1051By similarity
    Active sitei124 – 1241By similarity

    GO - Molecular functioni

    • glyoxalase (glycolic acid-forming) activity Source: ParkinsonsUK-UCL
    • glyoxalase III activity Source: ParkinsonsUK-UCL

    GO - Biological processi

    • cellular response to glyoxal Source: ParkinsonsUK-UCL
    • cellular response to methylglyoxal Source: ParkinsonsUK-UCL
    • glycolate biosynthetic process Source: ParkinsonsUK-UCL
    • glyoxal catabolic process Source: ParkinsonsUK-UCL
    • lactate biosynthetic process Source: GOC
    • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: ParkinsonsUK-UCL
    • negative regulation of neuron death Source: ParkinsonsUK-UCL
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione-independent glyoxalase DJR-1.21 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Protein DJ-1 homolog 2
    Gene namesi
    Name:djr-1.2Imported
    ORF Names:C49G7.11
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    Proteomesi
    • UP000001940 Componenti: Chromosome V

    Organism-specific databases

    WormBaseiC49G7.11; CE08869; WBGene00016789; djr-1.2.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: WormBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 186186Glutathione-independent glyoxalase DJR-1.2PRO_0000432109Add
    BLAST

    Proteomic databases

    EPDiO16228.
    PaxDbiO16228.
    PRIDEiO16228.

    Expressioni

    Tissue specificityi

    Expressed in various tissues, including pharyngeal muscles, pharynx-intestinal valve, ventral nerve cord, spermatheca, rectal gland, inner labial (IL) cells of head neurons, phasmid (PHA/PHB) neurons in tail and supporting sheath/socket cells, as well as in head mesodermal cells (HMC), excretory canals and coelomocytes.1 Publication

    Inductioni

    Induced by DAF-16 during starvation as well as in the dauer stage.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.C49G7.11.

    Structurei

    3D structure databases

    ProteinModelPortaliO16228.
    SMRiO16228. Positions 3-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C56 family. DJ-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG2764. Eukaryota.
    COG0693. LUCA.
    GeneTreeiENSGT00390000001231.
    HOGENOMiHOG000063194.
    InParanoidiO16228.
    OMAiAICASPR.
    OrthoDBiEOG7CVPZX.
    PhylomeDBiO16228.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    IPR006287. DJ1.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01383. not_thiJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O16228-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAAQKSALIL LPPEDAEEIE VIVTGDVLVR GGLQVLYAGS STEPVKCAKG
    60 70 80 90 100
    ARIVPDVALK DVKNKTFDII IIPGGPGCSK LAECPVIGEL LKTQVKSGGL
    110 120 130 140 150
    IGAICAGPTV LLAHGIVAER VTCHYTVKDK MTEGGYKYLD DNVVISDRVI
    160 170 180
    TSKGPGTAFE FALKIVETLE GPEKTNSLLK PLCLAK
    Length:186
    Mass (Da):19,556
    Last modified:January 1, 1998 - v1
    Checksum:iDE31E1AF30C011E3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO080906 Genomic DNA. Translation: CCD67692.1.
    PIRiT03871.
    RefSeqiNP_504132.1. NM_071731.1.
    UniGeneiCel.4466.

    Genome annotation databases

    EnsemblMetazoaiC49G7.11; C49G7.11; WBGene00016789.
    GeneIDi183625.
    KEGGicel:CELE_C49G7.11.
    UCSCiC49G7.11. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO080906 Genomic DNA. Translation: CCD67692.1.
    PIRiT03871.
    RefSeqiNP_504132.1. NM_071731.1.
    UniGeneiCel.4466.

    3D structure databases

    ProteinModelPortaliO16228.
    SMRiO16228. Positions 3-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi6239.C49G7.11.

    Proteomic databases

    EPDiO16228.
    PaxDbiO16228.
    PRIDEiO16228.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiC49G7.11; C49G7.11; WBGene00016789.
    GeneIDi183625.
    KEGGicel:CELE_C49G7.11.
    UCSCiC49G7.11. c. elegans.

    Organism-specific databases

    CTDi183625.
    WormBaseiC49G7.11; CE08869; WBGene00016789; djr-1.2.

    Phylogenomic databases

    eggNOGiKOG2764. Eukaryota.
    COG0693. LUCA.
    GeneTreeiENSGT00390000001231.
    HOGENOMiHOG000063194.
    InParanoidiO16228.
    OMAiAICASPR.
    OrthoDBiEOG7CVPZX.
    PhylomeDBiO16228.

    Miscellaneous databases

    NextBioi921786.
    PROiO16228.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiIPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    IPR006287. DJ1.
    [Graphical view]
    PfamiPF01965. DJ-1_PfpI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01383. not_thiJ. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. "DJR-1.2 of Caenorhabditis elegans is induced by DAF-16 in the dauer state."
      Lee J.Y., Kim C., Kim J., Park C.
      Gene 524:373-376(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiDJ12_CAEEL
    AccessioniPrimary (citable) accession number: O16228
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: January 1, 1998
    Last modified: May 11, 2016
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.