O16099 (MAL2_DROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maltase 2
EC=3.2.1.20

Gene names

Name:	Mal-B2
Synonyms:	Mav2
ORF Names:	GJ22506

Organism

Drosophila virilis (Fruit fly) [Complete proteome]

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Drosophila › virilis group

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence AAB82328.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 594	564	Maltase 2		PRO_0000001450
Sites
	Active site	241	1	Nucleophile By similarity
	Active site	310	1	Proton donor By similarity
	Site	377	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Glycosylation	537	1	N-linked (GlcNAc...) Potential
	Glycosylation	546	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	263	1	D → N in AAB82328. Ref.2
	Sequence conflict	554 – 555	2	PQ → L in AAB82328. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O16099 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 0AB84967886D3785	FASTA	594	67,586
	10 20 30 40 50 60 MAAAHQLTRT TQLLLLCSLL WTQAPRPVMS SLLSAQTEDF IDWWQHAVFY QIYPRSFKDS 70 80 90 100 110 120 NGDGIGDLQG VISKLPYLAE TGITATWLSP IFQSPMVDFG YDVSDYKSIQ TEYGTMADFE 130 140 150 160 170 180 QLVNTATSLG IKIILDFVPN HTSDKHEWFI KSAARDPLYD NFYVWADGKL DNQGVRQPPN 190 200 210 220 230 240 NWQSVFYGSA WQWHEQRGQY YLHQFAKEQP DLNFRNPAVV RAMDDVLLFW LNKGVAGFRI 250 260 270 280 290 300 DALNHLFEDE TLPDEPLSGK TTDPLSYDYT KHIYTKDLPE VLSMVQHWRQ LLDDYTAKHS 310 320 330 340 350 360 EGATRIMMTE AYADLQVLMD YYEDAGGVRG SQLPFNFHFI TDVSGDSDAR DFVYNIEKWL 370 380 390 400 410 420 IYMPRGHTAN WVMGNHDKPR VATRFGPASV DAMNMLLLTL PGVAVTYNGE ELGMQDYDEI 430 440 450 460 470 480 SWEDTVDPPA RIAGKLDYKK VSRDPERTPF QWSNATNAGF STAAKTWLPV NPNYLVLNLE 490 500 510 520 530 540 AQKQAVKSHY KVYKSLIELR KLPVLRRGRF SIEPLSRTVF AFKRTLKDYD TLVTIINVSA 550 560 570 580 590 KEQLVNLTDF INRPQKLVVE VAGVDSVYAP GQTISSSALT LSAHEGLICK LLDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15010-1051.87.
[2]	"The evolution of small gene clusters: evidence for an independent origin of the maltase gene cluster in Drosophila virilis and Drosophila melanogaster." Vieira C.P., Vieira J., Hartl D.L. Mol. Biol. Evol. 14:985-993(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-562. Strain: 9.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH940649 Genomic DNA. Translation: EDW64447.1. AF006573 Genomic DNA. Translation: AAB82328.1. Sequence problems.
RefSeq	XP_002052292.1. XM_002052256.1.
3D structure databases
ProteinModelPortal	O16099.
ModBase	Search...
Protein-protein interaction databases
STRING	7244.FBpp0236923.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0238431; FBpp0236923; FBgn0022838.
GeneID	6627886.
KEGG	dvi:Dvir_GJ22506.
Organism-specific databases
FlyBase	FBgn0022838. Dvir\Mal-B2.
Phylogenomic databases
eggNOG	COG0366.
InParanoid	O16099.
KO	K01187.
OMA	CIRYTNP.
OrthoDB	EOG48PK1D.
Family and domain databases
Gene3D	3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MAL2_DROVI

Accession

Primary (citable) accession number: O16099
Secondary accession number(s): B4LQJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project