O16027 (G3P1_GLORO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1
Short name=GAPDH-1
EC=1.2.1.12

Gene names

Name:

GPD-1

Organism

Globodera rostochiensis (Golden nematode worm)

Taxonomic identifier

31243 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Tylenchida › Tylenchina › Tylenchoidea › Heteroderidae › Heteroderinae › Globodera

Protein attributes

Sequence length	324 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›324	›324	Glyceraldehyde-3-phosphate dehydrogenase 1		PRO_0000145516
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	157 – 159	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	217 – 218	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	158	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	85	1	NAD; via carbonyl oxygen By similarity
	Binding site	188	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	240	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	322	1	NAD By similarity
Experimental info
	Non-terminal residue	324	1

Sequences

	Sequence		Length	Mass (Da)
	O16027 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1815C606CBBE1039	FASTA	324	34,580
	10 20 30 40 50 60 MVKPKVGING FGRIGRLALR AAVEKDTVQV VAINDPFIEL DYMVYMFNYD STHGRFNGKI 70 80 90 100 110 120 STSAGNLVVE KEGKATHTIK VFNLKDPAEI KWAEVGAEYV IESTGVFTTI EKASAHLKGG 130 140 150 160 170 180 AKKVVISAPS ADAPMYVMGV NEDKYDPAKD NVISNASCTT NCLAPLAKVI NDEFGIIEGL 190 200 210 220 230 240 MTTVHAVTAT QKTVDGPNGK QWRDGRGAAQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR 250 260 270 280 290 300 VPTPNVSVVD LTARLEKPAS LDAIKAAVKK AAEGNLKGIL GYTEDQVVST DFLGDSRSSI 310 320 FDAGACISLN PHFVKLVSWY DNEF

References

[1]

"Cloning of a trans-spliced glyceraldehyde-3-phosphate-dehydrogenase gene from the potato cyst nematode Globodera rostochiensis and expression of its putative promoter region in Caenorhabditis elegans." Qin L., Smant G., Stokkermans J.P.W.G., Bakker J., Schots A., Helder J. Mol. Biochem. Parasitol. 96:59-67(1998) [PubMed: 9851607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Ro1 / Mierenbos.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004522 mRNA. Translation: AAC79129.1.
3D structure databases
ProteinModelPortal	O16027.
SMR	O16027. Positions 3-324.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_GLORO

Accession

Primary (citable) accession number: O16027

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)