O16005 (OPSD_SEPOF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhodopsin

Gene names

Name:

RHO

Organism

Sepia officinalis (Common cuttlefish)

Taxonomic identifier

6610 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Cephalopoda › Coleoidea › Neocoleoidea › Decapodiformes › Sepiida › Sepiina › Sepiidae › Sepia

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Rhodopsin		PRO_0000197747
Regions
	Topological domain	1 – 33	33	Extracellular Potential
	Transmembrane	34 – 58	25	Helical; Name=1; Potential
	Topological domain	59 – 70	12	Cytoplasmic Potential
	Transmembrane	71 – 97	27	Helical; Name=2; Potential
	Topological domain	98 – 111	14	Extracellular Potential
	Transmembrane	112 – 131	20	Helical; Name=3; Potential
	Topological domain	132 – 151	20	Cytoplasmic Potential
	Transmembrane	152 – 175	24	Helical; Name=4; Potential
	Topological domain	176 – 199	24	Extracellular Potential
	Transmembrane	200 – 227	28	Helical; Name=5; Potential
	Topological domain	228 – 261	34	Cytoplasmic Potential
	Transmembrane	262 – 285	24	Helical; Name=6; Potential
	Topological domain	286 – 293	8	Extracellular Potential
	Transmembrane	294 – 318	25	Helical; Name=7; Potential
	Topological domain	319 – 464	146	Cytoplasmic Potential
	Compositional bias	370 – 380	11	Met-rich
	Compositional bias	381 – 464	84	Gln/Pro-rich
Amino acid modifications
	Modified residue	305	1	N6-(retinylidene)lysine By similarity
	Lipidation	336	1	S-palmitoyl cysteine By similarity
	Lipidation	337	1	S-palmitoyl cysteine By similarity
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 186		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O16005 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E90CB72E07E54E33	FASTA	464	51,506
	10 20 30 40 50 60 MGRDIPDNET WWYNPTMEVH PHWKQFNQVP DAVYYSLGIF IGICGIIGCT GNGIVIYLFT 70 80 90 100 110 120 KTKSLQTPAN MFIINLAFSD FTFSLVNGFP LMTISCFIKK WVFGMAACKV YGFIGGIFGL 130 140 150 160 170 180 MSIMTMSMIS IDRYNVIGRP MAASKKMSHR RAFLMIIFVW MWSTLWSIGP IFGWGAYVLE 190 200 210 220 230 240 GVLCNCSFDY ITRDSATRSN IVCMYIFAFC FPILIIFFCY FNIVMAVSNH EKEMAAMAKR 250 260 270 280 290 300 LNAKELRKAQ AGASAEMKLA KISIVIVTQF LLSWSPYAVV ALLAQFGPIE WVTPYAAQLP 310 320 330 340 350 360 VMFAKASAIH NPLIYSVSHP KFREAIAENF PWIITCCQFD EKEVEDDKDA ETEIPATEQS 370 380 390 400 410 420 GGESADAAQM KEMMAMMQKM QQQQAAYPPQ GAYPPQGGYP PQGYPPPPAQ GGYPPQGYPP 430 440 450 460 PPQGYPPAQG YPPQGYPPPQ GAPPQGAPPQ AAPPQGVDNQ AYQA

References

[1]	"The rhodopsin gene of the cuttlefish Sepia officinalis: sequence and spectral tuning." Bellingham J., Morris A.G., Hunt D.M. J. Exp. Biol. 201:2299-2306(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF000947 mRNA. Translation: AAC26329.1.
3D structure databases
ProteinModelPortal	O16005.
SMR	O16005. Positions 4-358.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. IPR027430. Retinal_BS. IPR006031. XYPPX. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF02162. XYPPX. 5 hits. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSD_SEPOF

Accession

Primary (citable) accession number: O16005

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)