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Protein

Syntaxin-1A homolog

Gene

unc-64

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a critical role in several secretory processes, including cuticle secretion and neurotransmitter release, and probably assists in neuronal membrane maturation or the final stages of neuronal differentiation. Essential for embryonic viability and development. Has a role in dauer formation and adult life span. Required for locomotion.3 Publications

GO - Molecular functioni

  • chaperone binding Source: WormBase
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central
  • structural constituent of cuticle Source: UniProtKB-KW

GO - Biological processi

  • calcium-mediated signaling Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • chemical synaptic transmission Source: UniProtKB
  • insulin receptor signaling pathway Source: UniProtKB
  • intracellular protein transport Source: GO_Central
  • locomotion Source: UniProtKB
  • nervous system development Source: UniProtKB-KW
  • neurotransmitter secretion Source: UniProtKB
  • regulation of exocytosis Source: InterPro
  • regulation of multicellular organism growth Source: UniProtKB
  • synaptic transmission, cholinergic Source: WormBase
  • synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
  • vesicle docking Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-449147. Signaling by Interleukins.

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-1A homolog
Alternative name(s):
Uncoordinated protein 64
Gene namesi
Name:unc-64Imported
ORF Names:F56A8.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF56A8.7a; CE16127; WBGene00006798; unc-64.
F56A8.7b; CE28035; WBGene00006798; unc-64.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 266266CytoplasmicSequence analysisAdd
BLAST
Transmembranei267 – 28721Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini288 – 2914ExtracellularSequence analysis

GO - Cellular componenti

  • axon Source: WormBase
  • basolateral plasma membrane Source: WormBase
  • dendrite Source: WormBase
  • integral component of membrane Source: GO_Central
  • membrane Source: UniProtKB
  • neuronal cell body Source: WormBase
  • SNARE complex Source: GO_Central
  • synaptic vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cuticle, Membrane

Pathology & Biotechi

Disruption phenotypei

Worms exhibit defects in locomotion and postembryonic development. All mutants are resistant to the acetylcholinesterase inhibitor aldicarb indicating impaired cholinergic transmission.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi248 – 2481A → V in e246; causes constitutive dauer formation, defects in locomotion and increases life span. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Syntaxin-1A homologPRO_0000314061Add
BLAST

Proteomic databases

PaxDbiO16000.
PRIDEiO16000.

Expressioni

Tissue specificityi

Expressed throughout the head ganglion, nerve ring, ventral cord, dorsal cord, intestine, vulva and spermatheca.2 Publications

Gene expression databases

BgeeiWBGene00006798.

Interactioni

Subunit structurei

Interacts with unc-18.1 Publication

GO - Molecular functioni

  • chaperone binding Source: WormBase
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi41914. 2 interactions.
DIPiDIP-25166N.
DIP-29202N.
IntActiO16000. 1 interaction.
MINTiMINT-1119100.
STRINGi6239.F56A8.7b.

Structurei

3D structure databases

ProteinModelPortaliO16000.
SMRiO16000. Positions 30-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini193 – 25563t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili69 – 9527Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Sequence analysis
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
InParanoidiO16000.
KOiK08486.
OMAiLIQYIPG.
OrthoDBiEOG091G0EUK.
PhylomeDBiO16000.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b3 Publications (identifier: O16000-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKDRLSALK AAQSEDEQDD DMHMDTGNAQ YMEEFFEQVE EIRGSVDIIA
60 70 80 90 100
NNVEEVKKKH SAILSNPVND QKTKEELDEL MAVIKRAANK VRGKLKLIEN
110 120 130 140 150
AIDHDEQGAG NADLRIRKTQ HSTLSRRFVE VMTDYNKTQT DYRERCKGRI
160 170 180 190 200
QRQLDIAGKQ VGDEDLEEMI ESGNPGVFTQ GIITDTQQAK QTLADIEARH
210 220 230 240 250
NDIMKLESSI RELHDMFMDM AMLVESQGEM VDRIEYNVEH AKEFVDRAVA
260 270 280 290
DTKKAVQYQS KARRKKICIL VTGVILITGL IIFILFYAKV L
Length:291
Mass (Da):33,253
Last modified:January 1, 1998 - v1
Checksum:iA1EC04D6F04A3613
GO
Isoform a3 Publications (identifier: O16000-2) [UniParc]FASTAAdd to basket
Also known as: syn1a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     268-291: CILVTGVILITGLIIFILFYAKVL → IILIVVTILIGFVSLWLIQYIPGI

Show »
Length:291
Mass (Da):33,314
Checksum:iA23E47EA538B6E69
GO
Isoform c1 Publication (identifier: O16000-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     265-291: KKICILVTGVILITGLIIFILFYAKVL → MKCYIFVLIV...NTSSQRFRVR

Show »
Length:319
Mass (Da):36,244
Checksum:iFBF05795E880EA5F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei265 – 29127KKICI…YAKVL → MKCYIFVLIVVIILVIVIAV VIWVIVANASTSVVLPSKTS SSSTSNTSSQRFRVR in isoform c. 1 PublicationVSP_052631Add
BLAST
Alternative sequencei268 – 29124CILVT…YAKVL → IILIVVTILIGFVSLWLIQY IPGI in isoform a. 3 PublicationsVSP_052630Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008842 mRNA. Translation: BAA23584.1.
AB008843 mRNA. Translation: BAA23585.1.
AB008844 mRNA. Translation: BAA23586.1.
AF047885 mRNA. Translation: AAD10538.1.
AF047886 mRNA. Translation: AAD10539.1.
AF047887 mRNA. Translation: AAD10540.1.
Z83230 Genomic DNA. Translation: CAB05747.1.
Z83230 Genomic DNA. Translation: CAC42303.1.
PIRiT37265.
T37266.
T42641.
RefSeqiNP_001022614.1. NM_001027443.3. [O16000-2]
NP_001022615.1. NM_001027444.3. [O16000-1]
UniGeneiCel.18377.

Genome annotation databases

EnsemblMetazoaiF56A8.7b; F56A8.7b; WBGene00006798. [O16000-1]
GeneIDi176743.
KEGGicel:CELE_F56A8.7.
UCSCiF56A8.7b. c. elegans. [O16000-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008842 mRNA. Translation: BAA23584.1.
AB008843 mRNA. Translation: BAA23585.1.
AB008844 mRNA. Translation: BAA23586.1.
AF047885 mRNA. Translation: AAD10538.1.
AF047886 mRNA. Translation: AAD10539.1.
AF047887 mRNA. Translation: AAD10540.1.
Z83230 Genomic DNA. Translation: CAB05747.1.
Z83230 Genomic DNA. Translation: CAC42303.1.
PIRiT37265.
T37266.
T42641.
RefSeqiNP_001022614.1. NM_001027443.3. [O16000-2]
NP_001022615.1. NM_001027444.3. [O16000-1]
UniGeneiCel.18377.

3D structure databases

ProteinModelPortaliO16000.
SMRiO16000. Positions 30-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41914. 2 interactions.
DIPiDIP-25166N.
DIP-29202N.
IntActiO16000. 1 interaction.
MINTiMINT-1119100.
STRINGi6239.F56A8.7b.

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Proteomic databases

PaxDbiO16000.
PRIDEiO16000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF56A8.7b; F56A8.7b; WBGene00006798. [O16000-1]
GeneIDi176743.
KEGGicel:CELE_F56A8.7.
UCSCiF56A8.7b. c. elegans. [O16000-1]

Organism-specific databases

CTDi176743.
WormBaseiF56A8.7a; CE16127; WBGene00006798; unc-64.
F56A8.7b; CE28035; WBGene00006798; unc-64.

Phylogenomic databases

eggNOGiKOG0810. Eukaryota.
COG5074. LUCA.
GeneTreeiENSGT00760000119200.
HOGENOMiHOG000286023.
InParanoidiO16000.
KOiK08486.
OMAiLIQYIPG.
OrthoDBiEOG091G0EUK.
PhylomeDBiO16000.

Enzyme and pathway databases

ReactomeiR-CEL-449147. Signaling by Interleukins.

Miscellaneous databases

PROiO16000.

Gene expression databases

BgeeiWBGene00006798.

Family and domain databases

InterProiIPR028669. STX1A/1B.
IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR19957:SF84. PTHR19957:SF84. 1 hit.
PfamiPF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX1A_CAEEL
AccessioniPrimary (citable) accession number: O16000
Secondary accession number(s): O18657, O61526, Q9TZZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.