O15991 (KLOM_EISFO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lombricine kinase Short name=LK EC=2.7.3.5
Organism	Eisenia foetida (Common brandling worm) (Common dung-worm)
Taxonomic identifier	6396 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Clitellata › Oligochaeta › Haplotaxida › Lumbricina › Lumbricidae › Eiseninae › Eisenia

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + lombricine = ADP + N-phospholombricine.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lombricine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Lombricine kinase		PRO_0000211985
Regions
	Domain	1 – 86	86	Phosphagen kinase N-terminal
	Domain	113 – 355	243	Phosphagen kinase C-terminal
	Nucleotide binding	116 – 120	5	ATP By similarity
	Nucleotide binding	308 – 313	6	ATP By similarity
Sites
	Binding site	179	1	ATP By similarity
	Binding site	224	1	ATP By similarity
	Binding site	280	1	ATP By similarity
	Binding site	323	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O15991 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8F8409D72F92ACB9	FASTA	371	41,781
	10 20 30 40 50 60 MPKFTARQNF PDYKSNGHKC MVGRHLTEDM YERLYELRTP NGVSIDKCIQ PSVDNTGRII 70 80 90 100 110 120 GLVAGDPESY EVFKELFDAV INEKHGGFGP TDKHPPPDLN ANALVGGQFD PKYVKSARIR 130 140 150 160 170 180 TGRSVKGFCL PPSISRAERR EVERIIVDAL AGLEGDLAGV YYPLKKMTPE QEKQLIADHF 190 200 210 220 230 240 LFQKPTGHLM VNSGAVRDWP DARGIWHNKD KTFLIWINEE DQVRIIAMQH GGDVKAVFER 250 260 270 280 290 300 FSRGLTQIEG LMKKHGHEFA WSERLGYICT CPSNLGTGLR ASVHLQLHKL SKHPKFEEII 310 320 330 340 350 360 LAFHLQKRGT GGEHTEAVDD VYDISNRARL KKSEREFVQL LIDGVGKLIE YEKLLEAGKS 370 IDDVLPASLK G

References

[1]

"Evolution of phosphagen kinase. VI. Isolation, characterization and cDNA-derived amino acid sequence of lombricine kinase from the earthworm Eisenia foetida, and identification of a possible candidate for the guanidine substrate recognition site." Suzuki T., Kawasaki Y., Furukohri T., Ellington W.R. Biochim. Biophys. Acta 1343:152-159(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB008013 mRNA. Translation: BAA22872.1.
3D structure databases
ProteinModelPortal	O15991.
SMR	O15991. Positions 2-368.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.135.10. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR000749. ATP-guanido_PTrfase. IPR022415. ATP-guanido_PTrfase_AS. IPR022414. ATP-guanido_PTrfase_cat. IPR022413. ATP-guanido_PTrfase_N. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
PANTHER	PTHR11547. PTHR11547. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
SUPFAM	SSF48034. ATP-gua_Ptrans. 1 hit.
PROSITE	PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLOM_EISFO

Accession

Primary (citable) accession number: O15991

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)