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O15910

- RIR2_TRYBB

UniProt

O15910 - RIR2_TRYBB

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Protein

Ribonucleoside-diphosphate reductase small chain

Gene

RNR2

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 2By similarity
Metal bindingi119 – 1191Iron 1PROSITE-ProRule annotation
Active sitei123 – 1231PROSITE-ProRule annotation
Metal bindingi178 – 1781Iron 2By similarity
Metal bindingi212 – 2121Iron 2By similarity
Metal bindingi215 – 2151Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R2 subunit
Ribonucleotide reductase small subunit
Gene namesi
Name:RNR2
Synonyms:NRDB
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Ribonucleoside-diphosphate reductase small chainPRO_0000190460Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliO15910.
SMRiO15910. Positions 16-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15910-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPKSHKCSR KEGEVEEPLL TENPDRYVIF PIKYPDIWQK YKEAESSIWT
60 70 80 90 100
VEEIDLGNDM TDWEKLDDGE RHFIKHVLAF FAASDGIVLE NLAERFMCEV
110 120 130 140 150
QVPEVRCFYG FQIAMENIHS ETYSVLIDTY VVDPDEKQRL LHAIRTIPCI
160 170 180 190 200
EKKAKWAIEW IGSQTSFPTR LVAFAAVEGI FFSGSFCAIF WLKKRGLMPG
210 220 230 240 250
LTFSNELISR DEGLHTDFAC LLYEKYIVNK LPRDRVLEII CNAVSIEREF
260 270 280 290 300
ICDALPVRLI GMNSQLMTQY IEFVADRLLV SLGYDRHYNS KNPFDFMDMI
310 320 330
SLQGKTNFFE KKVGEYQKAG VMSSERSSKV FSLDADF
Length:337
Mass (Da):39,009
Last modified:January 1, 1998 - v1
Checksum:iBD39919280C65CAE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 103CSR → RSA in CAA71741. (PubMed:9315738)Curated
Sequence conflicti43 – 431E → K in CAA71741. (PubMed:9315738)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80911 mRNA. Translation: AAB70705.1.
Y10768 Genomic DNA. Translation: CAA71741.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80911 mRNA. Translation: AAB70705.1 .
Y10768 Genomic DNA. Translation: CAA71741.1 .

3D structure databases

ProteinModelPortali O15910.
SMRi O15910. Positions 16-298.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0208.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the R1 and R2 subunits of ribonucleotide reductase from Trypanosoma brucei."
    Hofer A., Schmidt P.P., Graslund A., Thelander L.
    Proc. Natl. Acad. Sci. U.S.A. 94:6959-6964(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 427.
  2. "Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei."
    Dormeyer M., Schoneck R., Dittmar G.A.G., Krauth-Siegel R.L.
    FEBS Lett. 414:449-453(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRIR2_TRYBB
AccessioniPrimary (citable) accession number: O15910
Secondary accession number(s): O15880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3