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O15910

- RIR2_TRYBB

UniProt

O15910 - RIR2_TRYBB

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

RNR2

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi85 – 851Iron 1PROSITE-ProRule annotation
    Metal bindingi116 – 1161Iron 1PROSITE-ProRule annotation
    Metal bindingi116 – 1161Iron 2By similarity
    Metal bindingi119 – 1191Iron 1PROSITE-ProRule annotation
    Active sitei123 – 1231PROSITE-ProRule annotation
    Metal bindingi178 – 1781Iron 2By similarity
    Metal bindingi212 – 2121Iron 2By similarity
    Metal bindingi215 – 2151Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase R2 subunit
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:RNR2
    Synonyms:NRDB
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Ribonucleoside-diphosphate reductase small chainPRO_0000190460Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliO15910.
    SMRiO15910. Positions 16-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0208.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O15910-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPKSHKCSR KEGEVEEPLL TENPDRYVIF PIKYPDIWQK YKEAESSIWT    50
    VEEIDLGNDM TDWEKLDDGE RHFIKHVLAF FAASDGIVLE NLAERFMCEV 100
    QVPEVRCFYG FQIAMENIHS ETYSVLIDTY VVDPDEKQRL LHAIRTIPCI 150
    EKKAKWAIEW IGSQTSFPTR LVAFAAVEGI FFSGSFCAIF WLKKRGLMPG 200
    LTFSNELISR DEGLHTDFAC LLYEKYIVNK LPRDRVLEII CNAVSIEREF 250
    ICDALPVRLI GMNSQLMTQY IEFVADRLLV SLGYDRHYNS KNPFDFMDMI 300
    SLQGKTNFFE KKVGEYQKAG VMSSERSSKV FSLDADF 337
    Length:337
    Mass (Da):39,009
    Last modified:January 1, 1998 - v1
    Checksum:iBD39919280C65CAE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 103CSR → RSA in CAA71741. (PubMed:9315738)Curated
    Sequence conflicti43 – 431E → K in CAA71741. (PubMed:9315738)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80911 mRNA. Translation: AAB70705.1.
    Y10768 Genomic DNA. Translation: CAA71741.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80911 mRNA. Translation: AAB70705.1 .
    Y10768 Genomic DNA. Translation: CAA71741.1 .

    3D structure databases

    ProteinModelPortali O15910.
    SMRi O15910. Positions 16-298.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0208.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the R1 and R2 subunits of ribonucleotide reductase from Trypanosoma brucei."
      Hofer A., Schmidt P.P., Graslund A., Thelander L.
      Proc. Natl. Acad. Sci. U.S.A. 94:6959-6964(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 427.
    2. "Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei."
      Dormeyer M., Schoneck R., Dittmar G.A.G., Krauth-Siegel R.L.
      FEBS Lett. 414:449-453(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRIR2_TRYBB
    AccessioniPrimary (citable) accession number: O15910
    Secondary accession number(s): O15880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3