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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RNR1

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the large subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Allosteric activatorBy similarity1
Binding sitei58Allosteric activatorBy similarity1
Binding sitei93Allosteric activatorBy similarity1
Binding sitei212SubstrateBy similarity1
Sitei228Important for hydrogen atom transferBy similarity1
Sitei236Allosteric effector binding, determines substrate specificityBy similarity1
Binding sitei257Substrate; via amide nitrogenBy similarity1
Sitei266Allosteric effector binding, determines substrate specificityBy similarity1
Active sitei437Proton acceptorBy similarity1
Active sitei439Cysteine radical intermediateBy similarity1
Active sitei441Proton acceptorBy similarity1
Sitei454Important for hydrogen atom transferBy similarity1
Sitei759Important for electron transferBy similarity1
Sitei760Important for electron transferBy similarity1
Sitei833Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei836Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R1 subunit
Gene namesi
Name:RNR1
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

  • ribonucleoside-diphosphate reductase complex Source: GeneDB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001871991 – 838Ribonucleoside-diphosphate reductase large subunitAdd BLAST838

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi228 ↔ 454Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliO15909.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 97ATP-conePROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 22Allosteric activator bindingBy similarity7
Regioni227 – 228Substrate bindingBy similarity2
Regioni295 – 298Allosteric effector binding, determines substrate specificityBy similarity4
Regioni437 – 441Substrate bindingBy similarity5
Regioni625 – 629Substrate bindingBy similarity5

Sequence similaritiesi

Contains 1 ATP-cone domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLETVKLVTK RDGSVEPYDE KVVRSRIVNL MSGIDSYYVD VDDLVRVVGE
60 70 80 90 100
GVREGMSTSM LDELLAETAA YCVTKHPDYG LLAGRLAVTA LHKTTTESVL
110 120 130 140 150
DSFRVLHEHV SQATKRHAPL ISEELWDIAN KHSAALQQII NYERDFDFEY
160 170 180 190 200
FGYKTLERSY LLRVHKGRGV MEVVERPQQM FLRVALGIHG EDLERVKETY
210 220 230 240 250
DYMSQGFFTH ATPTLFNAGT PFPQMSSCFL VAMREDSIDG IYDTLKQCAI
260 270 280 290 300
ISKSAGGIGI HMHNIRAAGS YIAGTNGTSN GLVPMLRVWN NTARYVDQGG
310 320 330 340 350
GKRKGAFAIY LEPWHADIFG FLLLKKNTGK EDQRARDLFY GLWIPDLFME
360 370 380 390 400
RVESHGTWTL MDPNTAPFLS DCYGQEFTDL YERYEREGRG VRTIQAQELW
410 420 430 440 450
FLILESQVET GVPFMLYKDA CNFKSNQKNL GTIKCSNLCT EIVEYTSRDE
460 470 480 490 500
VAVCNLASIA LPRFVKDGAF DYVALKEVTK VVTRNLNRVI DRNHYPVCEA
510 520 530 540 550
RYSNLRHRPV GIGVQGLADA FALLSLPFAH PEAKKLNRQI FETIYIAAVE
560 570 580 590 600
ASTELAEKDG PYETFKGSPA SEGKLQFDLW DEERRIRGMN EDSVHSHCGL
610 620 630 640 650
WDWDSLKERV VKVGMRNSLL IAPMPTASTS QILGNNECIE PFTSNIYVRR
660 670 680 690 700
VLSGEFPVVN KHLVKELIRL RLWNDDMRRK IIALNGSVSG IKEIPERIRE
710 720 730 740 750
LYKVVWEIRQ KDLIDMAADR GRYIDQSQSL NLFLATPTSS QLTSMHFYSW
760 770 780 790 800
KKGLKTGMYY LRSQPAADAI KFTLDPKAMK ELPKPDKQSK EEVHGSVGRG
810 820 830
KRKRVGEKPT ANHSNAGAPN LNGPPDTDGD GGCLNCGS
Length:838
Mass (Da):94,619
Last modified:January 1, 1998 - v1
Checksum:i19A9B95525BAF69A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80910 mRNA. Translation: AAB70704.1.

Genome annotation databases

GeneDBiTb927.11.7840:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80910 mRNA. Translation: AAB70704.1.

3D structure databases

ProteinModelPortaliO15909.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiTb927.11.7840:pep.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_TRYBB
AccessioniPrimary (citable) accession number: O15909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.