O15757 (PP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1
EC=3.1.3.16

Alternative name(s):
DdPP1c

Gene names

Name:	pppB
ORF Names:	DDB_G0275619

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein phosphatase activity in vitro. Ref.1
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Enzyme regulation	Inhibited by okadaic acid, tautomycin and calyculin A. Inhibited by phosphatase inhibitor 2 (dpiA). Ref.1 Ref.4
Subunit structure	Interacts with dpiA. Ref.4
Developmental stage	Expressed throughout development. Ref.1
Sequence similarities	Belongs to the PPP phosphatase family.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Serine/threonine-protein phosphatase PP1		PRO_0000328376
Sites
	Active site	121	1	Proton donor By similarity
	Metal binding	60	1	Iron By similarity
	Metal binding	62	1	Iron By similarity
	Metal binding	88	1	Iron By similarity
	Metal binding	88	1	Manganese By similarity
	Metal binding	120	1	Manganese By similarity
	Metal binding	169	1	Manganese By similarity
	Metal binding	244	1	Manganese By similarity
Experimental info
	Mutagenesis	269	1	F → C: Five-fold increase in phosphatase activity, decreased binding efficiency to dpiA and reduced sensitivity to dpiA inhibition. Ref.1 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O15757 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 571EECA8BE113BA4	FASTA	321	36,939
	10 20 30 40 50 60 MEIDLDSIIT RLLEPRTTKP GKLVDLAEEE IRYLTVQATE IFINQPILLE LEAPIKICGD 70 80 90 100 110 120 IHGQYYDLLR LFEYGGFPPQ SNYLFLGDYV DRGKQSLETI CLLLAYKIKY PENFFILRGN 130 140 150 160 170 180 HECASINRIY GFYDECKRRY NSKLWKAFTD CFNCLPVAAI IDEKIFCMHG GLSPDLKNMD 190 200 210 220 230 240 QIRRITRPTV VPDFGLLCDL LWADPDKNIQ GWEDNDRGVS YTFGADVVES FLKKHDLDLV 250 260 270 280 290 300 CRAHQVVEDG YEFFAKRQLV TLFSAPNYFG EFDNAGAMMG VDETLMCSFQ ILKPADKKKL 310 320 TNDSNGRPLT PPRNKQQKPK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium discoideum protein phosphatase-1 catalytic subunit exhibits distinct biochemical properties." Andrioli L.P.M., Zaini P.A., Viviani W., da Silva A.M. Biochem. J. 373:703-711(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-269. Strain: AX4.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Identification and domain mapping of Dictyostelium discoideum type-1 protein phosphatase inhibitor-2." Sousa-Canavez J.M., Beton D., Gonzalez-Kristeller D.C., da Silva A.M. Biochimie 89:692-701(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION, INTERACTION WITH DPIA, MUTAGENESIS OF PHE-269. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020537 mRNA. Translation: AAB71415.1. AAFI02000013 Genomic DNA. Translation: EAL69560.1.
RefSeq	XP_643639.1. XM_638547.1.
3D structure databases
HSSP	HSSP built from PDB template 1FJM based on UniProtKB P62139.
ProteinModelPortal	O15757.
SMR	O15757. Positions 2-298.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185058.
Proteomic databases
PRIDE	O15757.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185058; DDB0185058; DDB_G0275619.
GeneID	8620226.
KEGG	ddi:DDB_G0275619.
Organism-specific databases
dictyBase	DDB_G0275619. pppB.
Phylogenomic databases
eggNOG	COG0639.
KO	K06269.
OMA	ADKKLYP.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP1_DICDI

Accession

Primary (citable) accession number: O15757
Secondary accession number(s): Q552P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)