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O15757 (PP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1

EC=3.1.3.16
Alternative name(s):
DdPP1c
Gene names
Name:pppB
ORF Names:DDB_G0275619
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase activity in vitro. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Enzyme regulation

Inhibited by okadaic acid, tautomycin and calyculin A. Inhibited by phosphatase inhibitor 2 (dpiA). Ref.1 Ref.4

Subunit structure

Interacts with dpiA. Ref.4

Developmental stage

Expressed throughout development. Ref.1

Sequence similarities

Belongs to the PPP phosphatase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321Serine/threonine-protein phosphatase PP1
PRO_0000328376

Sites

Active site1211Proton donor By similarity
Metal binding601Manganese 1 By similarity
Metal binding621Manganese 1 By similarity
Metal binding881Manganese 1 By similarity
Metal binding881Manganese 2 By similarity
Metal binding1201Manganese 2 By similarity
Metal binding1691Manganese 2 By similarity
Metal binding2441Manganese 2 By similarity

Experimental info

Mutagenesis2691F → C: Five-fold increase in phosphatase activity, decreased binding efficiency to dpiA and reduced sensitivity to dpiA inhibition. Ref.1 Ref.4

Sequences

Sequence LengthMass (Da)Tools
O15757 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 571EECA8BE113BA4

FASTA32136,939
        10         20         30         40         50         60 
MEIDLDSIIT RLLEPRTTKP GKLVDLAEEE IRYLTVQATE IFINQPILLE LEAPIKICGD 

        70         80         90        100        110        120 
IHGQYYDLLR LFEYGGFPPQ SNYLFLGDYV DRGKQSLETI CLLLAYKIKY PENFFILRGN 

       130        140        150        160        170        180 
HECASINRIY GFYDECKRRY NSKLWKAFTD CFNCLPVAAI IDEKIFCMHG GLSPDLKNMD 

       190        200        210        220        230        240 
QIRRITRPTV VPDFGLLCDL LWADPDKNIQ GWEDNDRGVS YTFGADVVES FLKKHDLDLV 

       250        260        270        280        290        300 
CRAHQVVEDG YEFFAKRQLV TLFSAPNYFG EFDNAGAMMG VDETLMCSFQ ILKPADKKKL 

       310        320 
TNDSNGRPLT PPRNKQQKPK K 

« Hide

References

« Hide 'large scale' references
[1]"Dictyostelium discoideum protein phosphatase-1 catalytic subunit exhibits distinct biochemical properties."
Andrioli L.P.M., Zaini P.A., Viviani W., da Silva A.M.
Biochem. J. 373:703-711(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-269.
Strain: AX4.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"Identification and domain mapping of Dictyostelium discoideum type-1 protein phosphatase inhibitor-2."
Sousa-Canavez J.M., Beton D., Gonzalez-Kristeller D.C., da Silva A.M.
Biochimie 89:692-701(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, INTERACTION WITH DPIA, MUTAGENESIS OF PHE-269.
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF020537 mRNA. Translation: AAB71415.1.
AAFI02000013 Genomic DNA. Translation: EAL69560.1.
RefSeqXP_643639.1. XM_638547.1.

3D structure databases

ProteinModelPortalO15757.
SMRO15757. Positions 2-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185058.

Proteomic databases

PRIDEO15757.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185058; DDB0185058; DDB_G0275619.
GeneID8620226.
KEGGddi:DDB_G0275619.

Organism-specific databases

dictyBaseDDB_G0275619. pppB.

Phylogenomic databases

eggNOGCOG0639.
OMAGSKPGQQ.
PhylomeDBO15757.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP1_DICDI
AccessionPrimary (citable) accession number: O15757
Secondary accession number(s): Q552P2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase