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Protein

Serine/threonine-protein phosphatase PP1

Gene

pppB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase activity in vitro.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by okadaic acid, tautomycin and calyculin A. Inhibited by phosphatase inhibitor 2 (dpiA).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1By similarity
Metal bindingi62 – 621Manganese 1By similarity
Metal bindingi88 – 881Manganese 1By similarity
Metal bindingi88 – 881Manganese 2By similarity
Metal bindingi120 – 1201Manganese 2By similarity
Active sitei121 – 1211Proton donorBy similarity
Metal bindingi169 – 1691Manganese 2By similarity
Metal bindingi244 – 2441Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.16. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 (EC:3.1.3.16)
Alternative name(s):
DdPP1c
Gene namesi
Name:pppB
ORF Names:DDB_G0275619
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275619. pppB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi269 – 2691F → C: Five-fold increase in phosphatase activity, decreased binding efficiency to dpiA and reduced sensitivity to dpiA inhibition. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Serine/threonine-protein phosphatase PP1PRO_0000328376Add
BLAST

Proteomic databases

PRIDEiO15757.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Interactioni

Subunit structurei

Interacts with dpiA.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0185058.

Structurei

3D structure databases

ProteinModelPortaliO15757.
SMRiO15757. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiO15757.
KOiK06269.
OMAiMALEIDI.
PhylomeDBiO15757.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIDLDSIIT RLLEPRTTKP GKLVDLAEEE IRYLTVQATE IFINQPILLE
60 70 80 90 100
LEAPIKICGD IHGQYYDLLR LFEYGGFPPQ SNYLFLGDYV DRGKQSLETI
110 120 130 140 150
CLLLAYKIKY PENFFILRGN HECASINRIY GFYDECKRRY NSKLWKAFTD
160 170 180 190 200
CFNCLPVAAI IDEKIFCMHG GLSPDLKNMD QIRRITRPTV VPDFGLLCDL
210 220 230 240 250
LWADPDKNIQ GWEDNDRGVS YTFGADVVES FLKKHDLDLV CRAHQVVEDG
260 270 280 290 300
YEFFAKRQLV TLFSAPNYFG EFDNAGAMMG VDETLMCSFQ ILKPADKKKL
310 320
TNDSNGRPLT PPRNKQQKPK K
Length:321
Mass (Da):36,939
Last modified:January 1, 1998 - v1
Checksum:i571EECA8BE113BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020537 mRNA. Translation: AAB71415.1.
AAFI02000013 Genomic DNA. Translation: EAL69560.1.
RefSeqiXP_643639.1. XM_638547.1.

Genome annotation databases

EnsemblProtistsiDDB0185058; DDB0185058; DDB_G0275619.
GeneIDi8620226.
KEGGiddi:DDB_G0275619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020537 mRNA. Translation: AAB71415.1.
AAFI02000013 Genomic DNA. Translation: EAL69560.1.
RefSeqiXP_643639.1. XM_638547.1.

3D structure databases

ProteinModelPortaliO15757.
SMRiO15757. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0185058.

Proteomic databases

PRIDEiO15757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0185058; DDB0185058; DDB_G0275619.
GeneIDi8620226.
KEGGiddi:DDB_G0275619.

Organism-specific databases

dictyBaseiDDB_G0275619. pppB.

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiO15757.
KOiK06269.
OMAiMALEIDI.
PhylomeDBiO15757.

Enzyme and pathway databases

BRENDAi3.1.3.16. 1939.

Miscellaneous databases

PROiO15757.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dictyostelium discoideum protein phosphatase-1 catalytic subunit exhibits distinct biochemical properties."
    Andrioli L.P.M., Zaini P.A., Viviani W., da Silva A.M.
    Biochem. J. 373:703-711(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-269.
    Strain: AX4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Identification and domain mapping of Dictyostelium discoideum type-1 protein phosphatase inhibitor-2."
    Sousa-Canavez J.M., Beton D., Gonzalez-Kristeller D.C., da Silva A.M.
    Biochimie 89:692-701(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, INTERACTION WITH DPIA, MUTAGENESIS OF PHE-269.
    Strain: AX4.

Entry informationi

Entry nameiPP1_DICDI
AccessioniPrimary (citable) accession number: O15757
Secondary accession number(s): Q552P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.