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O15757

- PP1_DICDI

UniProt

O15757 - PP1_DICDI

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Protein
Serine/threonine-protein phosphatase PP1
Gene
pppB, DDB_G0275619
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase activity in vitro.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Enzyme regulationi

Inhibited by okadaic acid, tautomycin and calyculin A. Inhibited by phosphatase inhibitor 2 (dpiA).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1 By similarity
Metal bindingi62 – 621Manganese 1 By similarity
Metal bindingi88 – 881Manganese 1 By similarity
Metal bindingi88 – 881Manganese 2 By similarity
Metal bindingi120 – 1201Manganese 2 By similarity
Active sitei121 – 1211Proton donor By similarity
Metal bindingi169 – 1691Manganese 2 By similarity
Metal bindingi244 – 2441Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 (EC:3.1.3.16)
Alternative name(s):
DdPP1c
Gene namesi
Name:pppB
ORF Names:DDB_G0275619
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275619. pppB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi269 – 2691F → C: Five-fold increase in phosphatase activity, decreased binding efficiency to dpiA and reduced sensitivity to dpiA inhibition. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Serine/threonine-protein phosphatase PP1
PRO_0000328376Add
BLAST

Proteomic databases

PRIDEiO15757.

Expressioni

Developmental stagei

Expressed throughout development.1 Publication

Interactioni

Subunit structurei

Interacts with dpiA.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0185058.

Structurei

3D structure databases

ProteinModelPortaliO15757.
SMRiO15757. Positions 2-298.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.

Phylogenomic databases

eggNOGiCOG0639.
KOiK06269.
OMAiGSKPGQQ.
PhylomeDBiO15757.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15757-1 [UniParc]FASTAAdd to Basket

« Hide

MEIDLDSIIT RLLEPRTTKP GKLVDLAEEE IRYLTVQATE IFINQPILLE    50
LEAPIKICGD IHGQYYDLLR LFEYGGFPPQ SNYLFLGDYV DRGKQSLETI 100
CLLLAYKIKY PENFFILRGN HECASINRIY GFYDECKRRY NSKLWKAFTD 150
CFNCLPVAAI IDEKIFCMHG GLSPDLKNMD QIRRITRPTV VPDFGLLCDL 200
LWADPDKNIQ GWEDNDRGVS YTFGADVVES FLKKHDLDLV CRAHQVVEDG 250
YEFFAKRQLV TLFSAPNYFG EFDNAGAMMG VDETLMCSFQ ILKPADKKKL 300
TNDSNGRPLT PPRNKQQKPK K 321
Length:321
Mass (Da):36,939
Last modified:January 1, 1998 - v1
Checksum:i571EECA8BE113BA4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020537 mRNA. Translation: AAB71415.1.
AAFI02000013 Genomic DNA. Translation: EAL69560.1.
RefSeqiXP_643639.1. XM_638547.1.

Genome annotation databases

EnsemblProtistsiDDB0185058; DDB0185058; DDB_G0275619.
GeneIDi8620226.
KEGGiddi:DDB_G0275619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020537 mRNA. Translation: AAB71415.1 .
AAFI02000013 Genomic DNA. Translation: EAL69560.1 .
RefSeqi XP_643639.1. XM_638547.1.

3D structure databases

ProteinModelPortali O15757.
SMRi O15757. Positions 2-298.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185058.

Proteomic databases

PRIDEi O15757.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185058 ; DDB0185058 ; DDB_G0275619 .
GeneIDi 8620226.
KEGGi ddi:DDB_G0275619.

Organism-specific databases

dictyBasei DDB_G0275619. pppB.

Phylogenomic databases

eggNOGi COG0639.
KOi K06269.
OMAi GSKPGQQ.
PhylomeDBi O15757.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dictyostelium discoideum protein phosphatase-1 catalytic subunit exhibits distinct biochemical properties."
    Andrioli L.P.M., Zaini P.A., Viviani W., da Silva A.M.
    Biochem. J. 373:703-711(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-269.
    Strain: AX4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "Identification and domain mapping of Dictyostelium discoideum type-1 protein phosphatase inhibitor-2."
    Sousa-Canavez J.M., Beton D., Gonzalez-Kristeller D.C., da Silva A.M.
    Biochimie 89:692-701(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, INTERACTION WITH DPIA, MUTAGENESIS OF PHE-269.
    Strain: AX4.

Entry informationi

Entry nameiPP1_DICDI
AccessioniPrimary (citable) accession number: O15757
Secondary accession number(s): Q552P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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