O15746 (RTCA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtca
Synonyms:	R2037, rtc1
ORF Names:	DDB_G0276159

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.
Subcellular location	Nucleus › nucleoplasm By similarity.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Probable RNA 3'-terminal phosphate cyclase		PRO_0000156413
Regions
	Nucleotide binding	328 – 332	5	ATP By similarity
Sites
	Active site	354	1	Tele-AMP-histidine intermediate By similarity
	Binding site	137	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O15746 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F3AEFBC044458CE9	FASTA	433	47,111
	10 20 30 40 50 60 MGKNKNYNKN QFKKSKTNND TTVAQQQQTI EEKPDFKIDG SILEGGGQIL RNSVALASLF 70 80 90 100 110 120 NKAISIEKIR YNRDQPGLKN QHKAGIDLMS RLFKAHLTGC SVGSCKLYYQ PTQKTIQDDG 130 140 150 160 170 180 VIEADTKTAG SICLMIQVSL PCLIFAPHST KMVLGGGTNC DFAPAADYIQ NVFLPIATTM 190 200 210 220 230 240 GFKCEMSIDK RGFYPKGGGA VTLTTQPLTQ PLSPITIVNK GEVNRIVIKS YFTSPRISPL 250 260 270 280 290 300 VAERMNNTAK KLIKKDFKKV DVETELIDVS KFSFGDGTFI EIRAYTDQGC IFGATGNGAI 310 320 330 340 350 360 GVPAEKVAED AANSLLKDLQ DGGCMDEYLQ DQLIIFMALA KGKSQIKTGP ISLHTQTSIH 370 380 390 400 410 420 ITSLMTGAIF TITPLTNNTQ SGEETNLITC EGISYFPSDL NNNNNNSNSN TTTTTTTTTI 430 STTTIDNQNS EEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Loomis W.F., Iranfar N. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020279 Genomic DNA. Translation: AAB70847.1. AAFI02000014 Genomic DNA. Translation: EAL69379.1.
RefSeq	XP_643263.1. XM_638171.1.
3D structure databases
ProteinModelPortal	O15746.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185053.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185053; DDB0185053; DDB_G0276159.
GeneID	8620306.
KEGG	ddi:DDB_G0276159.
Organism-specific databases
dictyBase	DDB_G0276159. rtc1.
Phylogenomic databases
eggNOG	COG0430.
KO	K01974.
OMA	RRGHYPK.
ProtClustDB	CLSZ2430913.
Family and domain databases
Gene3D	3.30.360.20. 1 hit. 3.65.10.20. 2 hits.
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
PANTHER	PTHR11096. PTHR11096. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_DICDI

Accession

Primary (citable) accession number: O15746
Secondary accession number(s): Q552F0, Q75JH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)