Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O15746

- RTCA_DICDI

UniProt

O15746 - RTCA_DICDI

Protein

Probable RNA 3'-terminal phosphate cyclase

Gene

rtca

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.By similarity

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei137 – 1371ATPBy similarity
    Active sitei354 – 3541Tele-AMP-histidine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi328 – 3325ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-EC

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
    Short name:
    RNA cyclase
    Short name:
    RNA-3'-phosphate cyclase
    Gene namesi
    Name:rtca
    Synonyms:R2037, rtc1
    ORF Names:DDB_G0276159
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0276159. rtc1.

    Subcellular locationi

    Nucleusnucleoplasm By similarity

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 433433Probable RNA 3'-terminal phosphate cyclasePRO_0000156413Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0185053.

    Structurei

    3D structure databases

    ProteinModelPortaliO15746.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0430.
    KOiK01974.
    OMAiAEMLLRN.
    PhylomeDBiO15746.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O15746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKNKNYNKN QFKKSKTNND TTVAQQQQTI EEKPDFKIDG SILEGGGQIL    50
    RNSVALASLF NKAISIEKIR YNRDQPGLKN QHKAGIDLMS RLFKAHLTGC 100
    SVGSCKLYYQ PTQKTIQDDG VIEADTKTAG SICLMIQVSL PCLIFAPHST 150
    KMVLGGGTNC DFAPAADYIQ NVFLPIATTM GFKCEMSIDK RGFYPKGGGA 200
    VTLTTQPLTQ PLSPITIVNK GEVNRIVIKS YFTSPRISPL VAERMNNTAK 250
    KLIKKDFKKV DVETELIDVS KFSFGDGTFI EIRAYTDQGC IFGATGNGAI 300
    GVPAEKVAED AANSLLKDLQ DGGCMDEYLQ DQLIIFMALA KGKSQIKTGP 350
    ISLHTQTSIH ITSLMTGAIF TITPLTNNTQ SGEETNLITC EGISYFPSDL 400
    NNNNNNSNSN TTTTTTTTTI STTTIDNQNS EEK 433
    Length:433
    Mass (Da):47,111
    Last modified:January 1, 1998 - v1
    Checksum:iF3AEFBC044458CE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020279 Genomic DNA. Translation: AAB70847.1.
    AAFI02000014 Genomic DNA. Translation: EAL69379.1.
    RefSeqiXP_643263.1. XM_638171.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185053; DDB0185053; DDB_G0276159.
    GeneIDi8620306.
    KEGGiddi:DDB_G0276159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020279 Genomic DNA. Translation: AAB70847.1 .
    AAFI02000014 Genomic DNA. Translation: EAL69379.1 .
    RefSeqi XP_643263.1. XM_638171.1.

    3D structure databases

    ProteinModelPortali O15746.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185053.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185053 ; DDB0185053 ; DDB_G0276159 .
    GeneIDi 8620306.
    KEGGi ddi:DDB_G0276159.

    Organism-specific databases

    dictyBasei DDB_G0276159. rtc1.

    Phylogenomic databases

    eggNOGi COG0430.
    KOi K01974.
    OMAi AEMLLRN.
    PhylomeDBi O15746.

    Miscellaneous databases

    PROi O15746.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Loomis W.F., Iranfar N.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AX3.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiRTCA_DICDI
    AccessioniPrimary (citable) accession number: O15746
    Secondary accession number(s): Q552F0, Q75JH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3