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O15743

- SPNA_DICDI

UniProt

O15743 - SPNA_DICDI

Protein

Protein spalten

Gene

spnA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in cell-type differentiation and morphogenesis. Dephosphorylates casein; in vitro. May also be involved as modulators or transducers in various transmembrane signaling systems.2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Enzyme regulationi

    Inhibited by 50 mM NaF (sodium fluoride).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi749 – 7491Manganese 1By similarity
    Metal bindingi750 – 7501Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi920 – 9201Manganese 2By similarity
    Metal bindingi963 – 9631Manganese 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi122 – 1298GTPBy similarity
    Nucleotide bindingi261 – 2677GTPBy similarity
    Nucleotide bindingi286 – 2905GTPBy similarity
    Nucleotide bindingi373 – 3764GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: dictyBase
    3. magnesium ion binding Source: dictyBase
    4. manganese ion binding Source: dictyBase
    5. protein serine/threonine phosphatase activity Source: dictyBase
    6. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. anatomical structure morphogenesis Source: dictyBase
    2. cell differentiation Source: dictyBase
    3. G-protein coupled receptor signaling pathway Source: InterPro
    4. protein dephosphorylation Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase, Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein spalten
    Including the following 2 domains:
    Probable guanine nucleotide-binding protein spalten
    Protein serine/threonine phosphatase spalten (EC:3.1.3.16)
    Gene namesi
    Name:spnA
    Synonyms:spn
    ORF Names:DDB_G0276155
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0276155. spnA.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Cell membrane 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. membrane Source: dictyBase
    3. plasma membrane Source: UniProtKB-SubCell
    4. ruffle Source: dictyBase

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Morphological arrest at the mound stage and a defect in cell-type differentiation. Instead of going through morphogenesis, after 16 hours of development, the mounds disaggregate to form smaller aggregates that eventually produce abnormal looking finger-like structures.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi373 – 3731N → D: Leads to the formation of very small-sized fruiting bodies. 1 Publication
    Mutagenesisi376 – 3761D → A: Leads to the formation of abnormal looking fruiting bodies. 1 Publication
    Mutagenesisi920 – 9201D → A: Loss of phosphatase activity; when associated with A-924. 1 Publication
    Mutagenesisi924 – 9241D → A: Loss of phosphatase activity; when associated with A-920. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 975975Protein spaltenPRO_0000367575Add
    BLAST

    Expressioni

    Developmental stagei

    Moderately expressed during growth. Increases during development, peaking at around 8 hours of development (mound stage) and then decreases gradually during the later stages. Mainly expressed in the prestalk cell population during the later multicellular stages. Required for prestalk specific gene expression and for prespore cell differentiation. Expressed (at protein level).

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

    Protein-protein interaction databases

    STRINGi44689.DDB_0185064.

    Structurei

    3D structure databases

    ProteinModelPortaliO15743.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini719 – 972254PP2C-likeAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili21 – 7050Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi31 – 5323Poly-GlnAdd
    BLAST
    Compositional biasi97 – 1037Poly-Asn
    Compositional biasi502 – 51211Poly-AsnAdd
    BLAST
    Compositional biasi561 – 57616Poly-ThrAdd
    BLAST
    Compositional biasi580 – 5856Poly-Asn
    Compositional biasi592 – 68695Lys-richAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the G-alpha family.Curated
    In the C-terminal section; belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0631.
    InParanoidiO15743.
    KOiK17500.
    OMAiEMDIISA.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    3.60.40.10. 1 hit.
    InterProiIPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00275. G_alpha. 1 hit.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O15743-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKMLFMNKK EKKEEQSPAH SSLAQQHQLA QQQYQLQQQQ LQLQYQQHQQ    50
    QLQLAQQQKQ NEQNLAQLST STSSNSSVNN TTNTNTNTTN SSSISSNNNN 100
    NNNTAVPILK AHDFCGTIMI LGHTESGKTT LQRQLEFIYG VTDPTDAKHY 150
    QRLIYGNTLA TLIRFIENSE RLNITLSPDN LARVKRIQSQ PVELARNRLP 200
    RFPLKLGWDC KCIWEDKVIQ SVYNHSKICS EIRTPGRPKY YMDRMFKVFD 250
    PSYTPTEMDI ISAYDQKDTI QSSAIIHKRF KVDLFGCSGK QSSPKNWVGL 300
    HQNYKPNYIF YVVALKDYFS DHLVATQNTD PTIVEMCNNH IHRNLLLESL 350
    NSFETLTKSE LFDKSLAIYL IFNTSDIFYE NIKQYDLKSC FSEYEGGNDP 400
    EKAVSFISNK FTKFLQNKDK PYKSHIVNLL DKNNVREEFE GIFDSLKIDA 450
    EKRGFTTPYN QSNSSPVSSI GSNSSRNSRL PNTSVSIPGL YSSDNDNTRL 500
    KNVNNNNNNN NNTTTYGSST FPSSVISTTG SISNSIASAM DNDSSYSNES 550
    SPTSSMTLLP TTTTTTTTTT TTATTTDSTN NNNNNATVVI GKGKPPKEPK 600
    PVKPPKEPKP PKEPKPPKEP KPPKEPKPIK EPKEKPVKES KPPKEPKPIK 650
    EPKESKEPKE PKEPKPTKPP KEKKTSKVDG AAESKKNGAD SCGNGGVGSK 700
    IKLESGFGSL QGRRKNMEDT HVILNNLMGA VTYNGPPKDI PISYYAVYDG 750
    HGGTETSTLL EPTVHNCLVN SQSFRDGDYE QAFRDAYAEA DDIVIEKCEK 800
    SGSTGVSALL VGNKLYTANV GDSEIVLARA QPNANPKGPV TYEPVLLSYK 850
    HLASDDQEKK RVTDLGGMII FNRLFGSLAV SRSFGDKEYK EGEKKFCVSD 900
    PYQTTTDLTA RDHFFILACD GLWDKVEYDE AVQFVQRNIK LGKSATEISE 950
    LLAQDSYDRG SGDNITVLVV ILNWN 975
    Length:975
    Mass (Da):109,016
    Last modified:January 1, 1998 - v1
    Checksum:iBBB322F5026D2A4F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019985 mRNA. Translation: AAB70844.1.
    AAFI02000014 Genomic DNA. Translation: EAL69377.1.
    PIRiT08606.
    RefSeqiXP_643266.1. XM_638174.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185064; DDB0185064; DDB_G0276155.
    GeneIDi8620309.
    KEGGiddi:DDB_G0276155.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF019985 mRNA. Translation: AAB70844.1 .
    AAFI02000014 Genomic DNA. Translation: EAL69377.1 .
    PIRi T08606.
    RefSeqi XP_643266.1. XM_638174.1.

    3D structure databases

    ProteinModelPortali O15743.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0185064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185064 ; DDB0185064 ; DDB_G0276155 .
    GeneIDi 8620309.
    KEGGi ddi:DDB_G0276155.

    Organism-specific databases

    dictyBasei DDB_G0276155. spnA.

    Phylogenomic databases

    eggNOGi COG0631.
    InParanoidi O15743.
    KOi K17500.
    OMAi EMDIISA.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    3.60.40.10. 1 hit.
    InterProi IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00275. G_alpha. 1 hit.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Spalten, a protein containing Galpha-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium."
      Aubry L., Firtel R.A.
      Genes Dev. 12:1525-1538(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, ENZYME REGULATION, MUTAGENESIS OF ASN-373; ASP-376; ASP-920 AND ASP-924, SUBCELLULAR LOCATION.
      Strain: AX3.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "The novel ankyrin-repeat containing kinase ARCK-1 acts as a suppressor of the Spalten signaling pathway during Dictyostelium development."
      Aubry L., Lee S., Ravanel K., Firtel R.A.
      Dev. Biol. 263:308-322(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSPNA_DICDI
    AccessioniPrimary (citable) accession number: O15743
    Secondary accession number(s): Q552E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3