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O15743

- SPNA_DICDI

UniProt

O15743 - SPNA_DICDI

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Protein

Protein spalten

Gene

spnA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in cell-type differentiation and morphogenesis. Dephosphorylates casein; in vitro. May also be involved as modulators or transducers in various transmembrane signaling systems.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by 50 mM NaF (sodium fluoride).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi749 – 7491Manganese 1By similarity
Metal bindingi750 – 7501Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi920 – 9201Manganese 2By similarity
Metal bindingi963 – 9631Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1298GTPBy similarity
Nucleotide bindingi261 – 2677GTPBy similarity
Nucleotide bindingi286 – 2905GTPBy similarity
Nucleotide bindingi373 – 3764GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: dictyBase
  3. magnesium ion binding Source: dictyBase
  4. manganese ion binding Source: dictyBase
  5. protein serine/threonine phosphatase activity Source: dictyBase
  6. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. anatomical structure morphogenesis Source: dictyBase
  2. cell differentiation Source: dictyBase
  3. G-protein coupled receptor signaling pathway Source: InterPro
  4. protein dephosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spalten
Including the following 2 domains:
Probable guanine nucleotide-binding protein spalten
Protein serine/threonine phosphatase spalten (EC:3.1.3.16)
Gene namesi
Name:spnA
Synonyms:spn
ORF Names:DDB_G0276155
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276155. spnA.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Cell membrane 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: dictyBase
  3. plasma membrane Source: UniProtKB-KW
  4. ruffle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Morphological arrest at the mound stage and a defect in cell-type differentiation. Instead of going through morphogenesis, after 16 hours of development, the mounds disaggregate to form smaller aggregates that eventually produce abnormal looking finger-like structures.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi373 – 3731N → D: Leads to the formation of very small-sized fruiting bodies. 1 Publication
Mutagenesisi376 – 3761D → A: Leads to the formation of abnormal looking fruiting bodies. 1 Publication
Mutagenesisi920 – 9201D → A: Loss of phosphatase activity; when associated with A-924. 1 Publication
Mutagenesisi924 – 9241D → A: Loss of phosphatase activity; when associated with A-920. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 975975Protein spaltenPRO_0000367575Add
BLAST

Expressioni

Developmental stagei

Moderately expressed during growth. Increases during development, peaking at around 8 hours of development (mound stage) and then decreases gradually during the later stages. Mainly expressed in the prestalk cell population during the later multicellular stages. Required for prestalk specific gene expression and for prespore cell differentiation. Expressed (at protein level).

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein-protein interaction databases

STRINGi44689.DDB_0185064.

Structurei

3D structure databases

ProteinModelPortaliO15743.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini719 – 972254PP2C-likeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili21 – 7050Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 5323Poly-GlnAdd
BLAST
Compositional biasi97 – 1037Poly-Asn
Compositional biasi502 – 51211Poly-AsnAdd
BLAST
Compositional biasi561 – 57616Poly-ThrAdd
BLAST
Compositional biasi580 – 5856Poly-Asn
Compositional biasi592 – 68695Lys-richAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the G-alpha family.Curated
In the C-terminal section; belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0631.
InParanoidiO15743.
KOiK17500.
OMAiEMDIISA.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
3.60.40.10. 1 hit.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00275. G_alpha. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15743-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKMLFMNKK EKKEEQSPAH SSLAQQHQLA QQQYQLQQQQ LQLQYQQHQQ
60 70 80 90 100
QLQLAQQQKQ NEQNLAQLST STSSNSSVNN TTNTNTNTTN SSSISSNNNN
110 120 130 140 150
NNNTAVPILK AHDFCGTIMI LGHTESGKTT LQRQLEFIYG VTDPTDAKHY
160 170 180 190 200
QRLIYGNTLA TLIRFIENSE RLNITLSPDN LARVKRIQSQ PVELARNRLP
210 220 230 240 250
RFPLKLGWDC KCIWEDKVIQ SVYNHSKICS EIRTPGRPKY YMDRMFKVFD
260 270 280 290 300
PSYTPTEMDI ISAYDQKDTI QSSAIIHKRF KVDLFGCSGK QSSPKNWVGL
310 320 330 340 350
HQNYKPNYIF YVVALKDYFS DHLVATQNTD PTIVEMCNNH IHRNLLLESL
360 370 380 390 400
NSFETLTKSE LFDKSLAIYL IFNTSDIFYE NIKQYDLKSC FSEYEGGNDP
410 420 430 440 450
EKAVSFISNK FTKFLQNKDK PYKSHIVNLL DKNNVREEFE GIFDSLKIDA
460 470 480 490 500
EKRGFTTPYN QSNSSPVSSI GSNSSRNSRL PNTSVSIPGL YSSDNDNTRL
510 520 530 540 550
KNVNNNNNNN NNTTTYGSST FPSSVISTTG SISNSIASAM DNDSSYSNES
560 570 580 590 600
SPTSSMTLLP TTTTTTTTTT TTATTTDSTN NNNNNATVVI GKGKPPKEPK
610 620 630 640 650
PVKPPKEPKP PKEPKPPKEP KPPKEPKPIK EPKEKPVKES KPPKEPKPIK
660 670 680 690 700
EPKESKEPKE PKEPKPTKPP KEKKTSKVDG AAESKKNGAD SCGNGGVGSK
710 720 730 740 750
IKLESGFGSL QGRRKNMEDT HVILNNLMGA VTYNGPPKDI PISYYAVYDG
760 770 780 790 800
HGGTETSTLL EPTVHNCLVN SQSFRDGDYE QAFRDAYAEA DDIVIEKCEK
810 820 830 840 850
SGSTGVSALL VGNKLYTANV GDSEIVLARA QPNANPKGPV TYEPVLLSYK
860 870 880 890 900
HLASDDQEKK RVTDLGGMII FNRLFGSLAV SRSFGDKEYK EGEKKFCVSD
910 920 930 940 950
PYQTTTDLTA RDHFFILACD GLWDKVEYDE AVQFVQRNIK LGKSATEISE
960 970
LLAQDSYDRG SGDNITVLVV ILNWN
Length:975
Mass (Da):109,016
Last modified:January 1, 1998 - v1
Checksum:iBBB322F5026D2A4F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF019985 mRNA. Translation: AAB70844.1.
AAFI02000014 Genomic DNA. Translation: EAL69377.1.
PIRiT08606.
RefSeqiXP_643266.1. XM_638174.1.

Genome annotation databases

EnsemblProtistsiDDB0185064; DDB0185064; DDB_G0276155.
GeneIDi8620309.
KEGGiddi:DDB_G0276155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF019985 mRNA. Translation: AAB70844.1 .
AAFI02000014 Genomic DNA. Translation: EAL69377.1 .
PIRi T08606.
RefSeqi XP_643266.1. XM_638174.1.

3D structure databases

ProteinModelPortali O15743.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185064 ; DDB0185064 ; DDB_G0276155 .
GeneIDi 8620309.
KEGGi ddi:DDB_G0276155.

Organism-specific databases

dictyBasei DDB_G0276155. spnA.

Phylogenomic databases

eggNOGi COG0631.
InParanoidi O15743.
KOi K17500.
OMAi EMDIISA.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
3.60.40.10. 1 hit.
InterProi IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00275. G_alpha. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Spalten, a protein containing Galpha-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium."
    Aubry L., Firtel R.A.
    Genes Dev. 12:1525-1538(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, ENZYME REGULATION, MUTAGENESIS OF ASN-373; ASP-376; ASP-920 AND ASP-924, SUBCELLULAR LOCATION.
    Strain: AX3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "The novel ankyrin-repeat containing kinase ARCK-1 acts as a suppressor of the Spalten signaling pathway during Dictyostelium development."
    Aubry L., Lee S., Ravanel K., Firtel R.A.
    Dev. Biol. 263:308-322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPNA_DICDI
AccessioniPrimary (citable) accession number: O15743
Secondary accession number(s): Q552E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3