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O15743

- SPNA_DICDI

UniProt

O15743 - SPNA_DICDI

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Protein
Protein spalten
Gene
spnA, spn, DDB_G0276155
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in cell-type differentiation and morphogenesis. Dephosphorylates casein; in vitro. May also be involved as modulators or transducers in various transmembrane signaling systems.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Enzyme regulationi

Inhibited by 50 mM NaF (sodium fluoride).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi749 – 7491Manganese 1 By similarity
Metal bindingi750 – 7501Manganese 1; via carbonyl oxygen By similarity
Metal bindingi920 – 9201Manganese 2 By similarity
Metal bindingi963 – 9631Manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1298GTP By similarity
Nucleotide bindingi261 – 2677GTP By similarity
Nucleotide bindingi286 – 2905GTP By similarity
Nucleotide bindingi373 – 3764GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: dictyBase
  2. GTPase activity Source: InterPro
  3. magnesium ion binding Source: dictyBase
  4. manganese ion binding Source: dictyBase
  5. protein serine/threonine phosphatase activity Source: dictyBase
  6. signal transducer activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: InterPro
  2. anatomical structure morphogenesis Source: dictyBase
  3. cell differentiation Source: dictyBase
  4. protein dephosphorylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spalten
Including the following 2 domains:
Probable guanine nucleotide-binding protein spalten
Protein serine/threonine phosphatase spalten (EC:3.1.3.16)
Gene namesi
Name:spnA
Synonyms:spn
ORF Names:DDB_G0276155
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276155. spnA.

Subcellular locationi

Cytoplasmcytosol. Cell membrane 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. membrane Source: dictyBase
  3. plasma membrane Source: UniProtKB-SubCell
  4. ruffle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Morphological arrest at the mound stage and a defect in cell-type differentiation. Instead of going through morphogenesis, after 16 hours of development, the mounds disaggregate to form smaller aggregates that eventually produce abnormal looking finger-like structures.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi373 – 3731N → D: Leads to the formation of very small-sized fruiting bodies. 1 Publication
Mutagenesisi376 – 3761D → A: Leads to the formation of abnormal looking fruiting bodies. 1 Publication
Mutagenesisi920 – 9201D → A: Loss of phosphatase activity; when associated with A-924. 1 Publication
Mutagenesisi924 – 9241D → A: Loss of phosphatase activity; when associated with A-920. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 975975Protein spalten
PRO_0000367575Add
BLAST

Expressioni

Developmental stagei

Moderately expressed during growth. Increases during development, peaking at around 8 hours of development (mound stage) and then decreases gradually during the later stages. Mainly expressed in the prestalk cell population during the later multicellular stages. Required for prestalk specific gene expression and for prespore cell differentiation. Expressed (at protein level).

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein-protein interaction databases

STRINGi44689.DDB_0185064.

Structurei

3D structure databases

ProteinModelPortaliO15743.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini719 – 972254PP2C-like
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili21 – 7050 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi31 – 5323Poly-Gln
Add
BLAST
Compositional biasi97 – 1037Poly-Asn
Compositional biasi502 – 51211Poly-Asn
Add
BLAST
Compositional biasi561 – 57616Poly-Thr
Add
BLAST
Compositional biasi580 – 5856Poly-Asn
Compositional biasi592 – 68695Lys-rich
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the G-alpha family.
In the C-terminal section; belongs to the PP2C family.
Contains 1 PP2C-like domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0631.
InParanoidiO15743.
KOiK17500.
OMAiEMDIISA.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
3.60.40.10. 1 hit.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00275. G_alpha. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15743-1 [UniParc]FASTAAdd to Basket

« Hide

MKKMLFMNKK EKKEEQSPAH SSLAQQHQLA QQQYQLQQQQ LQLQYQQHQQ    50
QLQLAQQQKQ NEQNLAQLST STSSNSSVNN TTNTNTNTTN SSSISSNNNN 100
NNNTAVPILK AHDFCGTIMI LGHTESGKTT LQRQLEFIYG VTDPTDAKHY 150
QRLIYGNTLA TLIRFIENSE RLNITLSPDN LARVKRIQSQ PVELARNRLP 200
RFPLKLGWDC KCIWEDKVIQ SVYNHSKICS EIRTPGRPKY YMDRMFKVFD 250
PSYTPTEMDI ISAYDQKDTI QSSAIIHKRF KVDLFGCSGK QSSPKNWVGL 300
HQNYKPNYIF YVVALKDYFS DHLVATQNTD PTIVEMCNNH IHRNLLLESL 350
NSFETLTKSE LFDKSLAIYL IFNTSDIFYE NIKQYDLKSC FSEYEGGNDP 400
EKAVSFISNK FTKFLQNKDK PYKSHIVNLL DKNNVREEFE GIFDSLKIDA 450
EKRGFTTPYN QSNSSPVSSI GSNSSRNSRL PNTSVSIPGL YSSDNDNTRL 500
KNVNNNNNNN NNTTTYGSST FPSSVISTTG SISNSIASAM DNDSSYSNES 550
SPTSSMTLLP TTTTTTTTTT TTATTTDSTN NNNNNATVVI GKGKPPKEPK 600
PVKPPKEPKP PKEPKPPKEP KPPKEPKPIK EPKEKPVKES KPPKEPKPIK 650
EPKESKEPKE PKEPKPTKPP KEKKTSKVDG AAESKKNGAD SCGNGGVGSK 700
IKLESGFGSL QGRRKNMEDT HVILNNLMGA VTYNGPPKDI PISYYAVYDG 750
HGGTETSTLL EPTVHNCLVN SQSFRDGDYE QAFRDAYAEA DDIVIEKCEK 800
SGSTGVSALL VGNKLYTANV GDSEIVLARA QPNANPKGPV TYEPVLLSYK 850
HLASDDQEKK RVTDLGGMII FNRLFGSLAV SRSFGDKEYK EGEKKFCVSD 900
PYQTTTDLTA RDHFFILACD GLWDKVEYDE AVQFVQRNIK LGKSATEISE 950
LLAQDSYDRG SGDNITVLVV ILNWN 975
Length:975
Mass (Da):109,016
Last modified:January 1, 1998 - v1
Checksum:iBBB322F5026D2A4F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF019985 mRNA. Translation: AAB70844.1.
AAFI02000014 Genomic DNA. Translation: EAL69377.1.
PIRiT08606.
RefSeqiXP_643266.1. XM_638174.1.

Genome annotation databases

EnsemblProtistsiDDB0185064; DDB0185064; DDB_G0276155.
GeneIDi8620309.
KEGGiddi:DDB_G0276155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF019985 mRNA. Translation: AAB70844.1 .
AAFI02000014 Genomic DNA. Translation: EAL69377.1 .
PIRi T08606.
RefSeqi XP_643266.1. XM_638174.1.

3D structure databases

ProteinModelPortali O15743.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185064 ; DDB0185064 ; DDB_G0276155 .
GeneIDi 8620309.
KEGGi ddi:DDB_G0276155.

Organism-specific databases

dictyBasei DDB_G0276155. spnA.

Phylogenomic databases

eggNOGi COG0631.
InParanoidi O15743.
KOi K17500.
OMAi EMDIISA.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
3.60.40.10. 1 hit.
InterProi IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00275. G_alpha. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Spalten, a protein containing Galpha-protein-like and PP2C domains, is essential for cell-type differentiation in Dictyostelium."
    Aubry L., Firtel R.A.
    Genes Dev. 12:1525-1538(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, FUNCTION, ENZYME REGULATION, MUTAGENESIS OF ASN-373; ASP-376; ASP-920 AND ASP-924, SUBCELLULAR LOCATION.
    Strain: AX3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "The novel ankyrin-repeat containing kinase ARCK-1 acts as a suppressor of the Spalten signaling pathway during Dictyostelium development."
    Aubry L., Lee S., Ravanel K., Firtel R.A.
    Dev. Biol. 263:308-322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPNA_DICDI
AccessioniPrimary (citable) accession number: O15743
Secondary accession number(s): Q552E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi