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O15697

- GAS8_TRYBR

UniProt

O15697 - GAS8_TRYBR

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Protein

Trypanin

Gene
N/A
Organism
Trypanosoma brucei rhodesiense
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Cytoskeletal linker that plays a central role in the flagellum cell motility. Required for directional cell motility. Plays a role as part of a dynein regulatory system that regulates flagellar beat in response to signals from the central pair apparatus and radial spokes in procyclic cells. Plays also an essential role in the bloodstream form of the trypanosomes as its silencing is lethal for the circulating form.3 Publications

GO - Biological processi

  1. cell motility Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trypanin
Alternative name(s):
T lymphocyte-triggering factor
Short name:
TLTF
OrganismiTrypanosoma brucei rhodesiense
Taxonomic identifieri31286 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton. Cell projectionciliumflagellum
Note: Associated with the cytoskeleton. Localized to the site of attachment of the flagellum to the subpellicular cytoskeleton. Also localized in the flagellum of the bloodstream form trypanosomes.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. microtubule Source: UniProtKB-KW
  3. motile cilium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453TrypaninPRO_0000220379Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO15697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili60 – 15697Sequence AnalysisAdd
BLAST
Coiled coili185 – 377193Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the GAS8 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR025593. GAS8.
[Graphical view]
PfamiPF13851. GAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15697 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MPPRTAAERG GRRKSVKAPP PVDPLVELTT LESVHDALAK AERLRNYFQV
60 70 80 90 100
ERDKVNDFWT ITKGEVETYR NRLFNAEASI EELERSHQVE MKVYKQRVRH
110 120 130 140 150
LIYERKKKAQ ACQDESDRLL REAEDRHLQR MNEIQAKLQQ QDQQLRAAAA
160 170 180 190 200
DHEMNVYEKR DSHSYMVTVT KTQSHEKELA RLQVSCEAKL KVLRDELELR
210 220 230 240 250
RRAEIHEIEE RKNEHINALI KQHEEKFHEM KTYYNQITTN NLEIIHSLKE
260 270 280 290 300
EIAQMKQNDE HNETLMYDID RENQNLVAPL EEAQREVAEL QQKRKQNEQN
310 320 330 340 350
KRGLEVTRVK LRSLREEIRR QREEHQALEE RYACVHRERE ELKGKFESAL
360 370 380 390 400
RQAVMVVEER NEVLQQKLIE SHALVEERDV QLEGVLRAMN LEPKTLELIA
410 420 430 440 450
TEVDEWLQRK NQLIKDLHFE LKKGEKLYSA TLLEMERRCQ TANIASLPRS

NFE
Length:453
Mass (Da):53,954
Last modified:January 1, 1998 - v1
Checksum:i842A5E2AAA384215
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012853 mRNA. Translation: AAB81499.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF012853 mRNA. Translation: AAB81499.1 .

3D structure databases

ProteinModelPortali O15697.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR025593. GAS8.
[Graphical view ]
Pfami PF13851. GAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The gene for a T lymphocyte triggering factor from African trypanosomes."
    Vaidya T., Bakhiet M., Hill K.L., Olsson T., Kristensson K., Donelson J.E.
    J. Exp. Med. 186:433-438(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "T lymphocyte-triggering factor of African trypanosomes is associated with the flagellar fraction of the cytoskeleton and represents a new family of proteins that are present in several divergent eukaryotes."
    Hill K.L., Hutchings N.R., Grandgenett P.M., Donelson J.E.
    J. Biol. Chem. 275:39369-39378(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes."
    Hutchings N.R., Donelson J.E., Hill K.L.
    J. Cell Biol. 156:867-877(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system."
    Ralston K.S., Lerner A.G., Diener D.R., Hill K.L.
    Eukaryot. Cell 5:696-711(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Trypanin, a component of the flagellar dynein regulatory complex, is essential in bloodstream form African trypanosomes."
    Ralston K.S., Hill K.L.
    PLoS Pathog. 2:873-882(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGAS8_TRYBR
AccessioniPrimary (citable) accession number: O15697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Procyclic parasite mutants lacking trypanin exhibit a motility defect indicative of abnormal dynein regulation, but have an actively beating flagellum and are able to spin and tumble in place. However, bloodstream form mutants lacking trypanin exhibit a severe growth defect and are not viable. They revealed a failure of cytokinesis.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3