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O15697

- GAS8_TRYBR

UniProt

O15697 - GAS8_TRYBR

Protein

Trypanin

Gene
N/A
Organism
Trypanosoma brucei rhodesiense
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Cytoskeletal linker that plays a central role in the flagellum cell motility. Required for directional cell motility. Plays a role as part of a dynein regulatory system that regulates flagellar beat in response to signals from the central pair apparatus and radial spokes in procyclic cells. Plays also an essential role in the bloodstream form of the trypanosomes as its silencing is lethal for the circulating form.3 Publications

    GO - Biological processi

    1. cell motility Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypanin
    Alternative name(s):
    T lymphocyte-triggering factor
    Short name:
    TLTF
    OrganismiTrypanosoma brucei rhodesiense
    Taxonomic identifieri31286 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton. Cell projectionciliumflagellum
    Note: Associated with the cytoskeleton. Localized to the site of attachment of the flagellum to the subpellicular cytoskeleton. Also localized in the flagellum of the bloodstream form trypanosomes.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. microtubule Source: UniProtKB-KW
    3. motile cilium Source: InterPro

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 453453TrypaninPRO_0000220379Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO15697.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili60 – 15697Sequence AnalysisAdd
    BLAST
    Coiled coili185 – 377193Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GAS8 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR025593. GAS8.
    [Graphical view]
    PfamiPF13851. GAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O15697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPRTAAERG GRRKSVKAPP PVDPLVELTT LESVHDALAK AERLRNYFQV    50
    ERDKVNDFWT ITKGEVETYR NRLFNAEASI EELERSHQVE MKVYKQRVRH 100
    LIYERKKKAQ ACQDESDRLL REAEDRHLQR MNEIQAKLQQ QDQQLRAAAA 150
    DHEMNVYEKR DSHSYMVTVT KTQSHEKELA RLQVSCEAKL KVLRDELELR 200
    RRAEIHEIEE RKNEHINALI KQHEEKFHEM KTYYNQITTN NLEIIHSLKE 250
    EIAQMKQNDE HNETLMYDID RENQNLVAPL EEAQREVAEL QQKRKQNEQN 300
    KRGLEVTRVK LRSLREEIRR QREEHQALEE RYACVHRERE ELKGKFESAL 350
    RQAVMVVEER NEVLQQKLIE SHALVEERDV QLEGVLRAMN LEPKTLELIA 400
    TEVDEWLQRK NQLIKDLHFE LKKGEKLYSA TLLEMERRCQ TANIASLPRS 450
    NFE 453
    Length:453
    Mass (Da):53,954
    Last modified:January 1, 1998 - v1
    Checksum:i842A5E2AAA384215
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012853 mRNA. Translation: AAB81499.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012853 mRNA. Translation: AAB81499.1 .

    3D structure databases

    ProteinModelPortali O15697.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR025593. GAS8.
    [Graphical view ]
    Pfami PF13851. GAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene for a T lymphocyte triggering factor from African trypanosomes."
      Vaidya T., Bakhiet M., Hill K.L., Olsson T., Kristensson K., Donelson J.E.
      J. Exp. Med. 186:433-438(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "T lymphocyte-triggering factor of African trypanosomes is associated with the flagellar fraction of the cytoskeleton and represents a new family of proteins that are present in several divergent eukaryotes."
      Hill K.L., Hutchings N.R., Grandgenett P.M., Donelson J.E.
      J. Biol. Chem. 275:39369-39378(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes."
      Hutchings N.R., Donelson J.E., Hill K.L.
      J. Cell Biol. 156:867-877(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. "Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system."
      Ralston K.S., Lerner A.G., Diener D.R., Hill K.L.
      Eukaryot. Cell 5:696-711(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Trypanin, a component of the flagellar dynein regulatory complex, is essential in bloodstream form African trypanosomes."
      Ralston K.S., Hill K.L.
      PLoS Pathog. 2:873-882(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGAS8_TRYBR
    AccessioniPrimary (citable) accession number: O15697
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Procyclic parasite mutants lacking trypanin exhibit a motility defect indicative of abnormal dynein regulation, but have an actively beating flagellum and are able to spin and tumble in place. However, bloodstream form mutants lacking trypanin exhibit a severe growth defect and are not viable. They revealed a failure of cytokinesis.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3