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O15697 (GAS8_TRYBR) Reviewed, UniProtKB/Swiss-Prot

Last modified July 24, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypanin
Alternative name(s):
T lymphocyte-triggering factor
Short name=TLTF
OrganismTrypanosoma brucei rhodesiense
Taxonomic identifier31286 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytoskeletal linker that plays a central role in the flagellum cell motility. Required for directional cell motility. Plays a role as part of a dynein regulatory system that regulates flagellar beat in response to signals from the central pair apparatus and radial spokes in procyclic cells. Plays also an essential role in the bloodstream form of the trypanosomes as its silencing is lethal for the circulating form. Ref.3 Ref.4 Ref.5

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton. Cell projectionciliumflagellum. Note: Associated with the cytoskeleton. Localized to the site of attachment of the flagellum to the subpellicular cytoskeleton. Also localized in the flagellum of the bloodstream form trypanosomes. Ref.2 Ref.3 Ref.5

Miscellaneous

Procyclic parasite mutants lacking trypanin exhibit a motility defect indicative of abnormal dynein regulation, but have an actively beating flagellum and are able to spin and tumble in place. However, bloodstream form mutants lacking trypanin exhibit a severe growth defect and are not viable. They revealed a failure of cytokinesis.

Sequence similarities

Belongs to the GAS8 family.

Ontologies

Keywords
   Cellular componentCell projection
Cilium
Cytoplasm
Cytoskeleton
Flagellum
Microtubule
   DomainCoiled coil
Gene Ontology (GO)
   Biological_processcell motility

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

motile cilium

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Trypanin
PRO_0000220379

Regions

Coiled coil60 – 15697 Potential
Coiled coil185 – 377193 Potential

Sequences

Sequence LengthMass (Da)Tools
O15697 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 842A5E2AAA384215

FASTA45353,954
        10         20         30         40         50         60 
MPPRTAAERG GRRKSVKAPP PVDPLVELTT LESVHDALAK AERLRNYFQV ERDKVNDFWT 

        70         80         90        100        110        120 
ITKGEVETYR NRLFNAEASI EELERSHQVE MKVYKQRVRH LIYERKKKAQ ACQDESDRLL 

       130        140        150        160        170        180 
REAEDRHLQR MNEIQAKLQQ QDQQLRAAAA DHEMNVYEKR DSHSYMVTVT KTQSHEKELA 

       190        200        210        220        230        240 
RLQVSCEAKL KVLRDELELR RRAEIHEIEE RKNEHINALI KQHEEKFHEM KTYYNQITTN 

       250        260        270        280        290        300 
NLEIIHSLKE EIAQMKQNDE HNETLMYDID RENQNLVAPL EEAQREVAEL QQKRKQNEQN 

       310        320        330        340        350        360 
KRGLEVTRVK LRSLREEIRR QREEHQALEE RYACVHRERE ELKGKFESAL RQAVMVVEER 

       370        380        390        400        410        420 
NEVLQQKLIE SHALVEERDV QLEGVLRAMN LEPKTLELIA TEVDEWLQRK NQLIKDLHFE 

       430        440        450 
LKKGEKLYSA TLLEMERRCQ TANIASLPRS NFE 

« Hide

References

[1]"The gene for a T lymphocyte triggering factor from African trypanosomes."
Vaidya T., Bakhiet M., Hill K.L., Olsson T., Kristensson K., Donelson J.E.
J. Exp. Med. 186:433-438(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"T lymphocyte-triggering factor of African trypanosomes is associated with the flagellar fraction of the cytoskeleton and represents a new family of proteins that are present in several divergent eukaryotes."
Hill K.L., Hutchings N.R., Grandgenett P.M., Donelson J.E.
J. Biol. Chem. 275:39369-39378(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes."
Hutchings N.R., Donelson J.E., Hill K.L.
J. Cell Biol. 156:867-877(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system."
Ralston K.S., Lerner A.G., Diener D.R., Hill K.L.
Eukaryot. Cell 5:696-711(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Trypanin, a component of the flagellar dynein regulatory complex, is essential in bloodstream form African trypanosomes."
Ralston K.S., Hill K.L.
PLoS Pathog. 2:873-882(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012853 mRNA. Translation: AAB81499.1.

3D structure databases

ProteinModelPortalO15697.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR025593. GAS8.
[Graphical view]
PfamPF13851. GAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAS8_TRYBR
AccessionPrimary (citable) accession number: O15697
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: January 1, 1998
Last modified: July 24, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families