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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk

Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase, glycosomal (PGI)
  3. ATP-dependent 6-phosphofructokinase (pfk)
  4. Fructose-bisphosphate aldolase, glycosomal (ALD)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATP; via amide nitrogenUniRule annotation1 Publication
Metal bindingi199 – 1991Magnesium; catalyticUniRule annotation
Sitei200 – 2001Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Binding sitei226 – 2261ATP1 Publication
Active sitei229 – 2291Proton acceptorUniRule annotation
Binding sitei325 – 3251SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi173 – 1742ATPUniRule annotation1 Publication
Nucleotide bindingi198 – 2014ATPUniRule annotation1 Publication
Nucleotide bindingi341 – 3433ATPUniRule annotation1 Publication

GO - Molecular functioni

  • 6-phosphofructokinase activity Source: GeneDB
  • ATP binding Source: GeneDB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 6-phosphate metabolic process Source: InterPro
  • glycolytic process Source: GeneDB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKO15648.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkUniRule annotation
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

  • Glycosome UniRule annotation1 Publication

GO - Cellular componenti

  • glycosome Source: GeneDB
Complete GO annotation...

Keywords - Cellular componenti

Glycosome, Peroxisome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5686.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487ATP-dependent 6-phosphofructokinasePRO_0000429723Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation2 Publications

Chemistry

BindingDBiO15648.

Structurei

Secondary structure

1
487
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 155Combined sources
Turni26 – 294Combined sources
Helixi42 – 443Combined sources
Helixi46 – 516Combined sources
Beta strandi52 – 554Combined sources
Beta strandi58 – 614Combined sources
Beta strandi75 – 773Combined sources
Beta strandi81 – 844Combined sources
Beta strandi88 – 936Combined sources
Helixi95 – 973Combined sources
Beta strandi99 – 1046Combined sources
Helixi112 – 12716Combined sources
Beta strandi130 – 1345Combined sources
Helixi138 – 1425Combined sources
Helixi144 – 1474Combined sources
Beta strandi151 – 1533Combined sources
Helixi155 – 1584Combined sources
Helixi161 – 1633Combined sources
Beta strandi164 – 1663Combined sources
Helixi178 – 18811Combined sources
Beta strandi191 – 1977Combined sources
Helixi199 – 21517Combined sources
Beta strandi220 – 2256Combined sources
Helixi241 – 26020Combined sources
Beta strandi265 – 2717Combined sources
Helixi278 – 28710Combined sources
Beta strandi291 – 2944Combined sources
Helixi302 – 31211Combined sources
Beta strandi317 – 3248Combined sources
Turni325 – 3284Combined sources
Helixi329 – 3313Combined sources
Helixi349 – 36214Combined sources
Turni363 – 3675Combined sources
Beta strandi368 – 3703Combined sources
Beta strandi372 – 3765Combined sources
Helixi379 – 3835Combined sources
Helixi389 – 40719Combined sources
Beta strandi410 – 4189Combined sources
Beta strandi421 – 4266Combined sources
Helixi427 – 4304Combined sources
Beta strandi433 – 4375Combined sources
Helixi442 – 4509Combined sources
Beta strandi454 – 4563Combined sources
Helixi458 – 48124Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HIGX-ray2.40A/B1-487[»]
3CVLX-ray2.15B481-487[»]
3F5MX-ray2.70A/B/C/D1-487[»]
ProteinModelPortaliO15648.
SMRiO15648. Positions 9-455.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15648.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 2293Substrate bindingUniRule annotation
Regioni272 – 2743Substrate bindingUniRule annotation
Regioni380 – 3834Substrate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi485 – 4873Peroxisomal targeting signalUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

O15648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVESRSRVT SKLVKAHRAM LNSVTQEDLK VDRLPGADYP NPSKKYSSRT
60 70 80 90 100
EFRDKTDYIM YNPRPRDEPS SENPVSVSPL LCELAAARSR IHFNPTETTI
110 120 130 140 150
GIVTCGGICP GLNDVIRSIT LTGINVYNVK RVIGFRFGYW GLSKKGSQTA
160 170 180 190 200
IELHRGRVTN IHHYGGTILG SSRGPQDPKE MVDTLERLGV NILFTVGGDG
210 220 230 240 250
TQRGALVISQ EAKRRGVDIS VFGVPKTIDN DLSFSHRTFG FQTAVEKAVQ
260 270 280 290 300
AIRAAYAEAV SANYGVGVVK LMGRDSGFIA AQAAVASAQA NICLVPENPI
310 320 330 340 350
SEQEVMSLLE RRFCHSRSCV IIVAEGFGQD WGRGSGGYDA SGNKKLIDIG
360 370 380 390 400
VILTEKVKAF LKANKSRYPD STVKYIDPSY MIRACPPSAN DALFCATLAT
410 420 430 440 450
LAVHEAMAGA TGCIIAMRHN NYILVPIKVA TSVRRVLDLR GQLWRQVREI
460 470 480
TVDLGSDVRL ARKLEIRREL EAINRNRDRL HEELAKL
Length:487
Mass (Da):53,517
Last modified:January 1, 1998 - v1
Checksum:i59357FF92ADA1FA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008186 Genomic DNA. Translation: AAC47836.1.

Genome annotation databases

GeneDBiTb927.3.3270:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008186 Genomic DNA. Translation: AAC47836.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HIGX-ray2.40A/B1-487[»]
3CVLX-ray2.15B481-487[»]
3F5MX-ray2.70A/B/C/D1-487[»]
ProteinModelPortaliO15648.
SMRiO15648. Positions 9-455.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiO15648.
ChEMBLiCHEMBL5686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiTb927.3.3270:pep.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
SABIO-RKO15648.

Miscellaneous databases

EvolutionaryTraceiO15648.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_TRYBB
AccessioniPrimary (citable) accession number: O15648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.