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Protein

Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial

Gene

ap33-3

Organism
Trichomonas vaginalis
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit alpha-2, mitochondrial (ALPHA-SCS2), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (TVAG_165340), Succinate--CoA ligase [ADP-forming] subunit alpha-3, mitochondrial (ALPHA-SCS3), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (TVAG_144730), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (ap33-2), Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrial (ALPHA-SCS1), Succinate--CoA ligase [ADP-forming] subunit beta, hydrogenosomal, Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (TVAG_259190), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (ap33-1), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (TVAG_183500), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (ap33-3)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Coenzyme AUniRule annotation1
Binding sitei171Substrate; shared with subunit betaUniRule annotation1
Active sitei262Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processTricarboxylic acid cycleUniRule annotation
LigandNucleotide-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
Gene namesi
Name:ap33-3Imported
OrganismiTrichomonas vaginalisImported
Taxonomic identifieri5722 [NCBI]
Taxonomic lineageiEukaryotaParabasaliaTrichomonadidaTrichomonadidaeTrichomonas

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

MitochondrionUniRule annotation

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO15569
SMRiO15569
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 111CoA_bindingInterPro annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 109Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1255 Eukaryota
COG0074 LUCA

Family and domain databases

Gene3Di3.40.50.261, 1 hit
HAMAPiMF_01988 Succ_CoA_alpha, 1 hit
InterProiView protein in InterPro
IPR017440 Cit_synth/succinyl-CoA_lig_AS
IPR033847 Citrt_syn/SCS-alpha_CS
IPR003781 CoA-bd
IPR005810 CoA_lig_alpha
IPR005811 CoA_ligase
IPR036291 NAD(P)-bd_dom_sf
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF02629 CoA_binding, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001553 SucCS_alpha, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01019 sucCoAalpha, 1 hit
PROSITEiView protein in PROSITE
PS01216 SUCCINYL_COA_LIG_1, 1 hit
PS00399 SUCCINYL_COA_LIG_2, 1 hit

Sequencei

Sequence statusi: Complete.

O15569-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSSFERNL HQPLLFIDKD TKVVIQGIGN QGQFHSRLMR QYGTKVVGAV
60 70 80 90 100
HPKKAGSIIA GLPIFKNMKE VVKRTDANAS LIFVPAPGAA AACIEAAQAG
110 120 130 140 150
MGLVVCITEH IPQHDMIKVK KVMKETGCQL IGPNCPGLIQ PGTHTKLGII
160 170 180 190 200
PTNIFNNGKI GIVSRSGTLT YEAAYATTLA GLGQSTVVGI GGDPFAGQLH
210 220 230 240 250
TDVVKRFAAD PQTEGIILIG EIGGTSEEDA AEWIAKTKLT QEKPVVAFIA
260 270 280 290 300
GATAPPGKRM GHSGAIVSGG KGTAEGKYKA LEAAGVRIAR HPGNMGKFIF

EEMKRMGKI
Length:309
Mass (Da):32,529
Last modified:January 1, 1998 - v1
Checksum:i3D7D17FA7AFFF0DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87098 mRNA Translation: AAC48339.1

Entry informationi

Entry nameiO15569_TRIVA
AccessioniPrimary (citable) accession number: O15569
Entry historyiIntegrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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