O15552 (FFAR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Free fatty acid receptor 2

Alternative name(s):
G-protein coupled receptor 43

Gene names

Name:	FFAR2
Synonyms:	GPR43

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Ref.5 Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen. Ref.5 Ref.6
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Lipid-binding
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.1. Source: ProtInc lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Free fatty acid receptor 2		PRO_0000069571
Regions
	Topological domain	1 – 12	12	Extracellular Potential
	Transmembrane	13 – 33	21	Helical; Name=1; Potential
	Topological domain	34 – 41	8	Cytoplasmic
	Transmembrane	42 – 62	21	Helical; Name=2; Potential
	Topological domain	63 – 84	22	Extracellular Potential
	Transmembrane	85 – 105	21	Helical; Name=3; Potential
	Topological domain	106 – 126	21	Cytoplasmic Potential
	Transmembrane	127 – 147	21	Helical; Name=4; Potential
	Topological domain	148 – 173	26	Extracellular Potential
	Transmembrane	174 – 194	21	Helical; Name=5; Potential
	Topological domain	195 – 219	25	Cytoplasmic Potential
	Transmembrane	220 – 240	21	Helical; Name=6; Potential
	Topological domain	241 – 255	15	Extracellular Potential
	Transmembrane	256 – 276	21	Helical; Name=7; Potential
	Topological domain	277 – 330	54	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	211	1	L → H. Corresponds to variant rs409093 [ dbSNP | Ensembl ].		VAR_011861
Experimental info
	Sequence conflict	227	1	T → M in AAH96201. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O15552 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F4A8AC6AFBDF1E90	FASTA	330	37,144
	10 20 30 40 50 60 MLPDWKSSLI LMAYIIIFLT GLPANLLALR AFVGRIRQPQ PAPVHILLLS LTLADLLLLL 70 80 90 100 110 120 LLPFKIIEAA SNFRWYLPKV VCALTSFGFY SSIYCSTWLL AGISIERYLG VAFPVQYKLS 130 140 150 160 170 180 RRPLYGVIAA LVAWVMSFGH CTIVIIVQYL NTTEQVRSGN EITCYENFTD NQLDVVLPVR 190 200 210 220 230 240 LELCLVLFFI PMAVTIFCYW RFVWIMLSQP LVGAQRRRRA VGLAVVTLLN FLVCFGPYNV 250 260 270 280 290 300 SHLVGYHQRK SPWWRSIAVV FSSLNASLDP LLFYFSSSVV RRAFGRGLQV LRNQGSSLLG 310 320 330 RRGKDTAEGT NEDRGVGQGE GMPSSDFTTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1." Sawzdargo M., George S.R., Nguyen T., Xu S., Kolakowski L.F. Jr., O'Dowd B.F. Biochem. Biophys. Res. Commun. 239:543-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Kaighin V.A., Martin A.L., Aronstam R.S. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids." Nilsson N.E., Kotarsky K., Owman C., Olde B. Biochem. Biophys. Res. Commun. 303:1047-1052(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids." Brown A.J., Goldsworthy S.M., Barnes A.A., Eilert M.M., Tcheang L., Daniels D., Muir A.I., Wigglesworth M.J., Kinghorn I., Fraser N.J., Pike N.B., Strum J.C., Steplewski K.M., Murdock P.R., Holder J.C., Marshall F.H., Szekeres P.G., Wilson S. , Ignar D.M., Foord S.M., Wise A., Dowell S.J. J. Biol. Chem. 278:11312-11319(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024690 Genomic DNA. Translation: AAB86713.1. AC002511 Genomic DNA. Translation: AAB67886.1. EU432114 Genomic DNA. Translation: ABY87913.1. BC095535 mRNA. Translation: AAH95535.1. BC096198 mRNA. Translation: AAH96198.1. BC096199 mRNA. Translation: AAH96199.1. BC096200 mRNA. Translation: AAH96200.1. BC096201 mRNA. Translation: AAH96201.1.
IPI	IPI00007366.
PIR	JC5717.
RefSeq	NP_005297.1. NM_005306.2.
UniGene	Hs.248056.
3D structure databases
ProteinModelPortal	O15552.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15552. 3 interactions.
STRING	9606.ENSP00000246549.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	O15552.
Proteomic databases
PaxDb	O15552.
PRIDE	O15552.
Protocols and materials databases
DNASU	2867.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000246549; ENSP00000246549; ENSG00000126262. ENST00000599180; ENSP00000473159; ENSG00000126262.
GeneID	2867.
KEGG	hsa:2867.
UCSC	uc002nzg.2. human.
Organism-specific databases
CTD	2867.
GeneCards	GC19P035940.
H-InvDB	HIX0027605.
HGNC	HGNC:4501. FFAR2.
HPA	HPA023985.
MIM	603823. gene.
neXtProt	NX_O15552.
PharmGKB	PA28890.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47521.
HOGENOM	HOG000026778.
HOVERGEN	HBG051811.
InParanoid	O15552.
KO	K04328.
OMA	TCYENFT.
OrthoDB	EOG4XSKQX.
PhylomeDB	O15552.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	O15552.
Bgee	O15552.
CleanEx	HS_FFAR2.
Genevestigator	O15552.
GermOnline	ENSG00000126262. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR013312. GPR40-rel_orph. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01904. GPR40FAMILY.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O15552.
ChEMBL	CHEMBL5493.
GenomeRNAi	2867.
NextBio	11305.
SOURCE	Search...

Entry information

Entry name

FFAR2_HUMAN

Accession

Primary (citable) accession number: O15552
Secondary accession number(s): B0M0J9 , Q4VAZ3, Q4VAZ5, Q4VBL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.