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O15547

- P2RX6_HUMAN

UniProt

O15547 - P2RX6_HUMAN

Protein

P2X purinoceptor 6

Gene

P2RX6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Receptor for ATP that acts as a ligand-gated ion channel.By similarity

    GO - Molecular functioni

    1. ATP binding Source: BHF-UCL
    2. channel activity Source: ProtInc
    3. extracellular ATP-gated cation channel activity Source: BHF-UCL
    4. identical protein binding Source: BHF-UCL
    5. purinergic nucleotide receptor activity Source: BHF-UCL
    6. transmembrane signaling receptor activity Source: ProtInc

    GO - Biological processi

    1. cation transport Source: RefGenome
    2. muscle contraction Source: ProtInc
    3. protein heterooligomerization Source: Ensembl
    4. protein homooligomerization Source: BHF-UCL
    5. purinergic nucleotide receptor signaling pathway Source: GOC
    6. signal transduction Source: ProtInc
    7. transmembrane transport Source: GOC
    8. transport Source: ProtInc

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    P2X purinoceptor 6
    Short name:
    P2X6
    Alternative name(s):
    ATP receptor
    P2XM
    Purinergic receptor
    Purinergic receptor P2X-like 1
    Gene namesi
    Name:P2RX6
    Synonyms:P2RXL1, P2X6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:8538. P2RX6.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: BHF-UCL
    2. cytoplasm Source: BHF-UCL
    3. dendritic spine Source: Ensembl
    4. integral component of nuclear inner membrane Source: RefGenome
    5. integral component of plasma membrane Source: ProtInc
    6. neuronal cell body Source: Ensembl
    7. postsynaptic density Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162398523.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441P2X purinoceptor 6PRO_0000161557Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei64 – 641Phosphotyrosine1 Publication
    Disulfide bondi127 ↔ 177By similarity
    Disulfide bondi138 ↔ 161By similarity
    Disulfide bondi144 ↔ 171By similarity
    Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi228 ↔ 238By similarity
    Disulfide bondi272 ↔ 281By similarity

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiO15547.
    PRIDEiO15547.

    PTM databases

    PhosphoSiteiO15547.

    Expressioni

    Tissue specificityi

    Expressed predominantly in skeletal muscle.

    Gene expression databases

    ArrayExpressiO15547.
    BgeeiO15547.
    CleanExiHS_P2RX6.
    GenevestigatoriO15547.

    Organism-specific databases

    HPAiHPA028776.
    HPA028777.

    Interactioni

    Subunit structurei

    Unlike most P2XRs, P2RX6 does not seem to form homotrimers or heterotrimers.1 Publication

    Protein-protein interaction databases

    BioGridi114575. 1 interaction.
    IntActiO15547. 1 interaction.
    STRINGi9606.ENSP00000416193.

    Structurei

    3D structure databases

    ProteinModelPortaliO15547.
    SMRiO15547. Positions 41-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini11 – 3929CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini61 – 333273ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini355 – 44187CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei334 – 35421Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the P2X receptor family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG47843.
    HOGENOMiHOG000232042.
    HOVERGENiHBG053086.
    InParanoidiO15547.
    KOiK05221.
    OMAiRCPEHPS.
    OrthoDBiEOG78PV92.
    PhylomeDBiO15547.
    TreeFamiTF328633.

    Family and domain databases

    Gene3Di2.60.490.10. 1 hit.
    InterProiIPR003049. P2X6_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view]
    PANTHERiPTHR10125. PTHR10125. 1 hit.
    PfamiPF00864. P2X_receptor. 1 hit.
    [Graphical view]
    PRINTSiPR01313. P2X6RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsiTIGR00863. P2X. 1 hit.
    PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O15547-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCPQLAGAGS MGSPGATTGW GLLDYKTEKY VMTRNWRVGA LQRLLQFGIV    50
    VYVVGWALLA KKGYQERDLE PQFSIITKLK GVSVTQIKEL GNRLWDVADF 100
    VKPPQGENVF FLVTNFLVTP AQVQGRCPEH PSVPLANCWV DEDCPEGEGG 150
    THSHGVKTGQ CVVFNGTHRT CEIWSWCPVE SGVVPSRPLL AQAQNFTLFI 200
    KNTVTFSKFN FSKSNALETW DPTYFKHCRY EPQFSPYCPV FRIGDLVAKA 250
    GGTFEDLALL GGSVGIRVHW DCDLDTGDSG CWPHYSFQLQ EKSYNFRTAT 300
    HWWEQPGVEA RTLLKLYGIR FDILVTGQAG KFGLIPTAVT LGTGAAWLGV 350
    VTFFCDLLLL YVDREAHFYW RTKYEEAKAP KATANSVWRE LALASQARLA 400
    ECLRRSSAPA PTATAAGSQT QTPGWPCPSS DTHLPTHSGS L 441
    Length:441
    Mass (Da):48,829
    Last modified:November 2, 2010 - v2
    Checksum:iEFD45BDC81234A64
    GO
    Isoform 2 (identifier: O15547-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         30-55: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:415
    Mass (Da):45,808
    Checksum:i3E32A59741527671
    GO

    Sequence cautioni

    The sequence AAF13303.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.
    The sequence AAF13303.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAH33488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAI09210.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAA22046.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAA22047.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti393 – 3931L → F in AAF13303. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti38 – 381V → G.
    Corresponds to variant rs2006846 [ dbSNP | Ensembl ].
    VAR_057664
    Natural varianti242 – 2421R → H.1 Publication
    Corresponds to variant rs2277838 [ dbSNP | Ensembl ].
    VAR_020338

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei30 – 5526Missing in isoform 2. 1 PublicationVSP_044799Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002059 Genomic DNA. Translation: BAA22047.1. Different initiation.
    AB002058 mRNA. Translation: BAA22046.1. Different initiation.
    CR456535 mRNA. Translation: CAG30421.1.
    AC002472 Genomic DNA. No translation available.
    BC033488 mRNA. Translation: AAH33488.1. Different initiation.
    BC109209 mRNA. Translation: AAI09210.1. Different initiation.
    AF065385 mRNA. Translation: AAF13303.1. Sequence problems.
    CCDSiCCDS13788.2. [O15547-1]
    CCDS54504.1. [O15547-2]
    RefSeqiNP_001153026.1. NM_001159554.1. [O15547-2]
    NP_005437.2. NM_005446.3. [O15547-1]
    UniGeneiHs.113275.

    Genome annotation databases

    EnsembliENST00000401443; ENSP00000385309; ENSG00000099957. [O15547-2]
    ENST00000413302; ENSP00000416193; ENSG00000099957. [O15547-1]
    GeneIDi9127.
    KEGGihsa:9127.
    UCSCiuc010gsu.1. human. [O15547-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    P2X receptor entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002059 Genomic DNA. Translation: BAA22047.1 . Different initiation.
    AB002058 mRNA. Translation: BAA22046.1 . Different initiation.
    CR456535 mRNA. Translation: CAG30421.1 .
    AC002472 Genomic DNA. No translation available.
    BC033488 mRNA. Translation: AAH33488.1 . Different initiation.
    BC109209 mRNA. Translation: AAI09210.1 . Different initiation.
    AF065385 mRNA. Translation: AAF13303.1 . Sequence problems.
    CCDSi CCDS13788.2. [O15547-1 ]
    CCDS54504.1. [O15547-2 ]
    RefSeqi NP_001153026.1. NM_001159554.1. [O15547-2 ]
    NP_005437.2. NM_005446.3. [O15547-1 ]
    UniGenei Hs.113275.

    3D structure databases

    ProteinModelPortali O15547.
    SMRi O15547. Positions 41-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114575. 1 interaction.
    IntActi O15547. 1 interaction.
    STRINGi 9606.ENSP00000416193.

    Chemistry

    BindingDBi O15547.
    ChEMBLi CHEMBL2670.
    GuidetoPHARMACOLOGYi 483.

    PTM databases

    PhosphoSitei O15547.

    Proteomic databases

    PaxDbi O15547.
    PRIDEi O15547.

    Protocols and materials databases

    DNASUi 9127.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000401443 ; ENSP00000385309 ; ENSG00000099957 . [O15547-2 ]
    ENST00000413302 ; ENSP00000416193 ; ENSG00000099957 . [O15547-1 ]
    GeneIDi 9127.
    KEGGi hsa:9127.
    UCSCi uc010gsu.1. human. [O15547-1 ]

    Organism-specific databases

    CTDi 9127.
    GeneCardsi GC22P021364.
    HGNCi HGNC:8538. P2RX6.
    HPAi HPA028776.
    HPA028777.
    MIMi 608077. gene.
    neXtProti NX_O15547.
    PharmGKBi PA162398523.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47843.
    HOGENOMi HOG000232042.
    HOVERGENi HBG053086.
    InParanoidi O15547.
    KOi K05221.
    OMAi RCPEHPS.
    OrthoDBi EOG78PV92.
    PhylomeDBi O15547.
    TreeFami TF328633.

    Miscellaneous databases

    GeneWikii P2RX6.
    GenomeRNAii 9127.
    NextBioi 34213.
    PROi O15547.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O15547.
    Bgeei O15547.
    CleanExi HS_P2RX6.
    Genevestigatori O15547.

    Family and domain databases

    Gene3Di 2.60.490.10. 1 hit.
    InterProi IPR003049. P2X6_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view ]
    PANTHERi PTHR10125. PTHR10125. 1 hit.
    Pfami PF00864. P2X_receptor. 1 hit.
    [Graphical view ]
    PRINTSi PR01313. P2X6RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsi TIGR00863. P2X. 1 hit.
    PROSITEi PS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of P2XM, a novel human P2X receptor gene regulated by p53."
      Urano T., Nishimori H., Han H., Furuhata T., Kimura Y., Nakamura Y., Tokino T.
      Cancer Res. 57:3281-3287(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The DNA sequence of human chromosome 22."
      Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
      , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
      Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-441 (ISOFORM 1), VARIANT HIS-242.
      Tissue: Brain.
    5. "Cloning and tissue distribution of a human cDNA encoding P2X6 purinoceptor."
      Cheng X., Jin H., Huang C.-C.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-441 (ISOFORM 1).
      Tissue: Skeletal muscle.
    6. "Identification of the phosphotyrosine proteome from thrombin activated platelets."
      Maguire P.B., Wynne K.J., Harney D.F., O'Donoghue N.M., Stephens G., Fitzgerald D.J.
      Proteomics 2:642-648(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-64.
    7. "Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize."
      Barrera N.P., Ormond S.J., Henderson R.M., Murrell-Lagnado R.D., Edwardson J.M.
      J. Biol. Chem. 280:10759-10765(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, GLYCOSYLATION.

    Entry informationi

    Entry nameiP2RX6_HUMAN
    AccessioniPrimary (citable) accession number: O15547
    Secondary accession number(s): F6V3D7
    , Q32MB6, Q58F04, Q6IC33, Q9UL50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3