O15539 (RGS5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 5
Short name=RGS5

Gene names

Name:

RGS5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha By similarity.
Subcellular location	Isoform 1: Cytoplasm Ref.4. Membrane Ref.4. Isoform 2: Cytoplasm Ref.4.
Sequence similarities	Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Molecular function	Signal transduction inhibitor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of G-protein coupled receptor protein signaling pathway Traceable author statement Ref.3. Source: ProtInc signal transduction Inferred from electronic annotation. Source: Compara termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O15539-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O15539-2)

Also known as: RGS5s;

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Note: Acts as an endogenous negative regulator of isoform 1.

Isoform 3 (identifier: O15539-3)

The sequence of this isoform differs from the canonical sequence as follows:
     128-128: E → EVGLW

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

Regulator of G-protein signaling 5

PRO_0000204188

Regions

Domain

64 – 180

117

RGS

Natural variations

Alternative sequence

1 – 108

108

Missing in isoform 2.

VSP_043094

Alternative sequence

128

1

E → EVGLW in isoform 3.

VSP_045086

Secondary structure

1

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181

Helix Strand Turn

Details...

Helix

55 – 58

4

Helix

60 – 63

4

Helix

65 – 70

6

Helix

72 – 84

13

Helix

88 – 101

14

Helix

108 – 119

12

Helix

133 – 141

9

Helix

149 – 151

3

Helix

152 – 164

13

Helix

166 – 172

7

Helix

174 – 180

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2E08CB0179DE7687	FASTA	181	20,946
	10 20 30 40 50 60 MCKGLAALPH SCLERAKEIK IKLGILLQKP DSVGDLVIPY NEKPEKPAKT QKTSLDEALQ 70 80 90 100 110 120 WRDSLDKLLQ NNYGLASFKS FLKSEFSEEN LEFWIACEDY KKIKSPAKMA EKAKQIYEEF 130 140 150 160 170 180 IQTEAPKEVN IDHFTKDITM KNLVEPSLSS FDMAQKRIHA LMEKDSLPRF VRSEFYQELI K
	Isoform 2 (RGS5s) [UniParc]. Checksum: 3B9DE08FBE577E5D	FASTA	73	8,664
	10 20 30 40 50 60 MAEKAKQIYE EFIQTEAPKE VNIDHFTKDI TMKNLVEPSL SSFDMAQKRI HALMEKDSLP 70 RFVRSEFYQE LIK
	Isoform 3 [UniParc]. Checksum: 3B1840D1AB0D8DC0	FASTA	185	21,402
	10 20 30 40 50 60 MCKGLAALPH SCLERAKEIK IKLGILLQKP DSVGDLVIPY NEKPEKPAKT QKTSLDEALQ 70 80 90 100 110 120 WRDSLDKLLQ NNYGLASFKS FLKSEFSEEN LEFWIACEDY KKIKSPAKMA EKAKQIYEEF 130 140 150 160 170 180 IQTEAPKEVG LWVNIDHFTK DITMKNLVEP SLSSFDMAQK RIHALMEKDS LPRFVRSEFY QELIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Chatterjee T.K., Fisher R.A. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Isolation, tissue expression, and chromosomal assignment of human RGS5, a novel G-protein signaling regulator gene." Seki N., Sugano S., Suzuki Y., Nakagawara A., Ohira M., Muramatsu M.-A., Saito T., Hori T. J. Hum. Genet. 43:202-205(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Neuroblastoma.
[3]	"Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling." Cismowski M.J., Takesono A., Ma C., Lizano J.S., Xie X., Fuernkranz H., Lanier S.M., Duzic E. Nat. Biotechnol. 17:878-883(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[4]	"Identification of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues." Liang Y., Li C., Gozamn V.W., Woodwrad D.F. FEBS J. 272:791-799(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION. Tissue: Eye.
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix.
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Melanoma.
[10]	"Solution structure of the RGS domain of regulator of G-protein signalling 5 (RGS 5)." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 44-180.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF030108 mRNA. Translation: AAB84001.1.
AB008109 mRNA. Translation: BAA22889.1.
AF159570 mRNA. Translation: AAD40957.1.
AJ891044 mRNA. Translation: CAI76926.1.
AF493929 mRNA. Translation: AAM12643.1.
BX537427 mRNA. Translation: CAD97669.1.
AL451063, AL499616, AL583850 Genomic DNA. Translation: CAH71851.1.
AL583850, AL451063, AL499616 Genomic DNA. Translation: CAH73764.1.
AL499616, AL451063, AL583850 Genomic DNA. Translation: CAI15095.1.
CH471067 Genomic DNA. Translation: EAW90731.1.
BC030059 mRNA. Translation: AAH30059.1.
BU172751 mRNA. No translation available.

IPI

IPI00183077.
IPI00977109.
IPI00984574.

RefSeq

NP_001182232.1. NM_001195303.2.
NP_001241677.1. NM_001254748.1.
NP_001241678.1. NM_001254749.1.
NP_003608.1. NM_003617.3.

UniGene

Hs.24950.
Hs.677623.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CRP	NMR	-	A	44-180	[»]

ProteinModelPortal

O15539.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000319308.

PTM databases

PhosphoSite

O15539.

Proteomic databases

PaxDb

O15539.

PRIDE

O15539.

Protocols and materials databases

DNASU

8490.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000313961; ENSP00000319308; ENSG00000143248.
ENST00000527988; ENSP00000432313; ENSG00000143248.
ENST00000530507; ENSP00000433001; ENSG00000143248.

GeneID

8490.

KEGG

hsa:8490.

UCSC

uc001gcn.3. human.

Organism-specific databases

CTD

8490.

GeneCards

GC01M163080.

HGNC

HGNC:10001. RGS5.

HPA

HPA001821.

MIM

603276. gene.

neXtProt

NX_O15539.

PharmGKB

PA34376.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG285261.

HOGENOM

HOG000233512.

HOVERGEN

HBG013233.

KO

K16449.

OMA

HSCLERA.

OrthoDB

EOG41RPW5.

PhylomeDB

O15539.

Gene expression databases

ArrayExpress

O15539.

Bgee

O15539.

CleanEx

HS_RGS5.

Genevestigator

O15539.

GermOnline

ENSG00000143248. Homo sapiens.

Family and domain databases

Gene3D

1.10.196.10. 1 hit.

InterPro

IPR000342. Regulat_G_prot_signal.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
[Graphical view]

Pfam

PF00615. RGS. 1 hit.
[Graphical view]

PRINTS

PR01301. RGSPROTEIN.

SMART

SM00315. RGS. 1 hit.
[Graphical view]

SUPFAM

SSF48097. Regulat_G_prot_signal_superfam. 1 hit.

PROSITE

PS50132. RGS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O15539.

GenomeRNAi

8490.

NextBio

31763.

SOURCE

Search...

Entry information

Entry name

RGS5_HUMAN

Accession

Primary (citable) accession number: O15539
Secondary accession number(s): E9PMP5, Q53XA9, Q599J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.