Reviewed, UniProtKB/Swiss-Prot O15523 (DDX3Y_HUMAN)

Names and origin

Protein names

Recommended name:
    ATP-dependent RNA helicase DDX3Y
    EC=3.6.1.-
Alternative name(s):
    DEAD box protein 3, Y-chromosomal

Gene names

Name:	DDX3Y
Synonyms:	DBY

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.
Subunit structure	May interact with TDRD3.
Subcellular location	Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner. Ref.4
Tissue specificity	Testis-specific. Expressed predominantly in spermatogonia. Ref.5
Involvement in disease	DDX3Y is deleted in severe non-obstructive hypospermatogenesis [MIM:400042].
Sequence similarities	Belongs to the DEAD box helicase family. DDX3/DED1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	ATP-dependent RNA helicase DDX3Y		PRO_0000055011
Regions
	Domain	209 – 401	193	Helicase ATP-binding
	Domain	412 – 573	162	Helicase C-terminal
	Nucleotide binding	198 – 205	8	ATP By similarity
	Nucleotide binding	222 – 229	8	ATP By similarity
	Motif	178 – 206	29	Q motif
	Motif	345 – 348	4	DEAD box
Amino acid modifications
	Modified residue	69	1	Phosphotyrosine Ref.3 Ref.6
	Modified residue	321	1	Phosphothreonine Ref.8
	Modified residue	592	1	Phosphoserine Ref.9
Experimental info
	Sequence conflict	46	1	R → K in AAC51832. Ref.1
	Sequence conflict	46	1	R → K in AAC51831. Ref.1
	Sequence conflict	219	1	M → V in AAC51832. Ref.1
	Sequence conflict	219	1	M → V in AAC51831. Ref.1
	Sequence conflict	628	1	N → D in AAC51832. Ref.1
	Sequence conflict	628	1	N → D in AAC51831. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15523-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 0C370E9367952AB6	FASTA	660	73,154
	10 20 30 40 50 60 MSHVVVKNDP ELDQQLANLD LNSEKQSGGA STASKGRYIP PHLRNREASK GFHDKDSSGW 70 80 90 100 110 120 SCSKDKDAYS SFGSRDSRGK PGYFSERGSG SRGRFDDRGR SDYDGIGNRE RPGFGRFERS 130 140 150 160 170 180 GHSRWCDKSV EDDWSKPLPP SERLEQELFS GGNTGINFEK YDDIPVEATG SNCPPHIENF 190 200 210 220 230 240 SDIDMGEIIM GNIELTRYTR PTPVQKHAIP IIKGKRDLMA CAQTGSGKTA AFLLPILSQI 250 260 270 280 290 300 YTDGPGEALK AVKENGRYGR RKQYPISLVL APTRELAVQI YEEARKFSYR SRVRPCVVYG 310 320 330 340 350 360 GADIGQQIRD LERGCHLLVA TPGRLVDMME RGKIGLDFCK YLVLDEADRM LDMGFEPQIR 370 380 390 400 410 420 RIVEQDTMPP KGVRHTMMFS ATFPKEIQML ARDFLDEYIF LAVGRVGSTS ENITQKVVWV 430 440 450 460 470 480 EDLDKRSFLL DILGATGSDS LTLVFVETKK GADSLEDFLY HEGYACTSIH GDRSQRDREE 490 500 510 520 530 540 ALHQFRSGKS PILVATAVAA RGLDISNVRH VINFDLPSDI EEYVHRIGRT GRVGNLGLAT 550 560 570 580 590 600 SFFNEKNMNI TKDLLDLLVE AKQEVPSWLE NMAYEHHYKG GSRGRSKSNR FSGGFGARDY 610 620 630 640 650 660 RQSSGSSSSG FGASRGSSSR SGGGGYGNSR GFGGGGYGGF YNSDGYGGNY NSQGVDWWGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional coherence of the human Y chromosome." Lahn B.T., Page D.C. Science 278:675-680(1997) [PubMed: 9381176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-69, MASS SPECTROMETRY. Tissue: T-cell.
[4]	"Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation." Sekiguchi T., Iida H., Fukumura J., Nishimoto T. Exp. Cell Res. 300:213-222(2004) [PubMed: 15383328] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"The AZFa gene DBY (DDX3Y) is widely transcribed but the protein is limited to the male germ cells by translation control." Ditton H.J., Zimmer J., Kamp C., Rajpert-De Meyts E., Vogt P.H. Hum. Mol. Genet. 13:2333-2341(2004) [PubMed: 15294876] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-69, MASS SPECTROMETRY.
[7]	"TDRD3, a novel Tudor domain-containing protein, localizes to cytoplasmic stress granules." Goulet I., Boisvenue S., Mokas S., Mazroui R., Cote J. Hum. Mol. Genet. 17:3055-3074(2008) [PubMed: 18632687] [Abstract] Cited for: INTERACTION WITH TDRD3.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-321, MASS SPECTROMETRY.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-592, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

GeneReviews

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF000985 mRNA. Translation: AAC51832.1. AF000984 mRNA. Translation: AAC51831.1. BC034942 mRNA. Translation: AAH34942.1.
IPI	IPI00293616.
RefSeq	NP_001116137.1. NP_004651.2. XP_001713749.1.
UniGene	Hs.99120
3D structure databases
HSSP	HSSP built from PDB template 1FUK based on UniProtKB P10081.
SMR	O15523. Positions 166-578.
ModBase	Search...
PTM databases
PhosphoSite	O15523.
Proteomic databases
PRIDE	O15523.
Genome annotation databases
Ensembl	ENSG00000067048. Homo sapiens. [Contig view]
GeneID	100130220. 8653.
KEGG	hsa:100130220. hsa:8653.
Organism-specific databases
GeneCards	GC0YP013454. GC0YP013525.
H-InvDB	HIX0028484.
HGNC	HGNC:2699. DDX3Y.
HPA	HPA001648. HPA005631.
MIM	400010. gene. 400042. phenotype.
Orphanet	1646. Chromosome Y deletion.
PharmGKB	PA27168.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O15523.
HOVERGEN	O15523.
OMA	O15523. GKRDLMA.
Gene expression databases
ArrayExpress	O15523.
Bgee	O15523.
CleanEx	HS_DDX3Y.
GermOnline	ENSG00000067048. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

DDX3Y_HUMAN

Accession

Primary (citable) accession number: O15523
Secondary accession number(s): Q8IYV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)