ID   NKX28_HUMAN             Reviewed;         239 AA.
AC   O15522; Q8IUT7;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 2.
DT   27-MAR-2024, entry version 185.
DE   RecName: Full=Homeobox protein Nkx-2.8;
DE   AltName: Full=Homeobox protein NK-2 homolog H;
GN   Name=NKX2-8; Synonyms=NKX-2.8, NKX2G, NKX2H;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=9446603; DOI=10.1074/jbc.273.5.2917;
RA   Apergis G.A., Crawford N., Ghosh D., Steppan C.M., Vorachek W.R., Wen P.,
RA   Locker J.;
RT   "A novel nk-2-related transcription factor associated with human fetal
RT   liver and hepatocellular carcinoma.";
RL   J. Biol. Chem. 273:2917-2925(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Tongue;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the NK-2 homeobox family. {ECO:0000305}.
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DR   EMBL; AF000295; AAC71081.1; -; mRNA.
DR   EMBL; AF000297; AAC71082.1; -; Genomic_DNA.
DR   EMBL; AF000296; AAC71082.1; JOINED; Genomic_DNA.
DR   EMBL; AK313537; BAG36314.1; -; mRNA.
DR   EMBL; BC041090; AAH41090.1; -; mRNA.
DR   CCDS; CCDS9660.1; -.
DR   RefSeq; NP_055175.2; NM_014360.3.
DR   AlphaFoldDB; O15522; -.
DR   SMR; O15522; -.
DR   STRING; 9606.ENSP00000258829; -.
DR   BioMuta; NKX2-8; -.
DR   EPD; O15522; -.
DR   MassIVE; O15522; -.
DR   PaxDb; 9606-ENSP00000258829; -.
DR   PeptideAtlas; O15522; -.
DR   ProteomicsDB; 48722; -.
DR   Antibodypedia; 9891; 232 antibodies from 28 providers.
DR   DNASU; 26257; -.
DR   Ensembl; ENST00000258829.6; ENSP00000258829.4; ENSG00000136327.7.
DR   GeneID; 26257; -.
DR   KEGG; hsa:26257; -.
DR   MANE-Select; ENST00000258829.6; ENSP00000258829.4; NM_014360.4; NP_055175.2.
DR   UCSC; uc001wtx.4; human.
DR   AGR; HGNC:16364; -.
DR   CTD; 26257; -.
DR   DisGeNET; 26257; -.
DR   GeneCards; NKX2-8; -.
DR   HGNC; HGNC:16364; NKX2-8.
DR   HPA; ENSG00000136327; Group enriched (brain, esophagus, testis).
DR   MIM; 603245; gene.
DR   neXtProt; NX_O15522; -.
DR   OpenTargets; ENSG00000136327; -.
DR   PharmGKB; PA38402; -.
DR   VEuPathDB; HostDB:ENSG00000136327; -.
DR   eggNOG; KOG0842; Eukaryota.
DR   GeneTree; ENSGT00940000161610; -.
DR   HOGENOM; CLU_049543_0_3_1; -.
DR   InParanoid; O15522; -.
DR   OMA; HMPRREP; -.
DR   OrthoDB; 461623at2759; -.
DR   PhylomeDB; O15522; -.
DR   TreeFam; TF351204; -.
DR   PathwayCommons; O15522; -.
DR   SignaLink; O15522; -.
DR   BioGRID-ORCS; 26257; 10 hits in 1146 CRISPR screens.
DR   GenomeRNAi; 26257; -.
DR   Pharos; O15522; Tbio.
DR   PRO; PR:O15522; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   RNAct; O15522; Protein.
DR   Bgee; ENSG00000136327; Expressed in C1 segment of cervical spinal cord and 49 other cell types or tissues.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0007409; P:axonogenesis; IEA:Ensembl.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0006351; P:DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0050673; P:epithelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0001889; P:liver development; NAS:UniProtKB.
DR   GO; GO:0030324; P:lung development; IEA:Ensembl.
DR   GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IDA:UniProtKB.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   PANTHER; PTHR24340; HOMEOBOX PROTEIN NKX; 1.
DR   PANTHER; PTHR24340:SF27; HOMEOBOX PROTEIN NKX-2.8; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; O15522; HS.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..239
FT                   /note="Homeobox protein Nkx-2.8"
FT                   /id="PRO_0000048943"
FT   DNA_BIND        84..143
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..87
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        55..74
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         16
FT                   /note="D -> A"
FT                   /id="VAR_003753"
FT   CONFLICT        16
FT                   /note="D -> G (in Ref. 1; AAC71081/AAC71082)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   239 AA;  25866 MW;  E227378CCD6E9D27 CRC64;
     MATSGRLSFT VRSLLDLPEQ DAQHLPRREP EPRAPQPDPC AAWLDSERGH YPSSDESSLE
     TSPPDSSQRP SARPASPGSD AEKRKKRRVL FSKAQTLELE RRFRQQRYLS APEREQLASL
     LRLTPTQVKI WFQNHRYKLK RARAPGAAES PDLAASAELH AAPGLLRRVV VPVLVRDGQP
     CGGGGGGEVG TAAAQEKCGA PPAAACPLPG YPAFGPGSAL GLFPAYQHLA SPALVSWNW
//