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Protein

DNA-directed RNA polymerase II subunit RPB4

Gene

POLR2D

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).By similarity1 Publication

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB
  • nucleotide binding Source: InterPro
  • translation initiation factor binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE during HIV infection.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB4
Short name:
RNA polymerase II subunit B4
Alternative name(s):
DNA-directed RNA polymerase II subunit D
RNA polymerase II 16 kDa subunit
Short name:
RPB16
Gene namesi
Name:POLR2D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000144231.10.
HGNCiHGNC:9191. POLR2D.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5433.
OpenTargetsiENSG00000144231.
PharmGKBiPA33511.

Polymorphism and mutation databases

BioMutaiPOLR2D.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739811 – 142DNA-directed RNA polymerase II subunit RPB4Add BLAST142

Proteomic databases

EPDiO15514.
MaxQBiO15514.
PaxDbiO15514.
PeptideAtlasiO15514.
PRIDEiO15514.

PTM databases

iPTMnetiO15514.
PhosphoSitePlusiO15514.

Expressioni

Gene expression databases

BgeeiENSG00000144231.
CleanExiHS_POLR2D.
ExpressionAtlasiO15514. baseline and differential.
GenevisibleiO15514. HS.

Organism-specific databases

HPAiHPA034694.
HPA046092.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111429. 71 interactors.
CORUMiO15514.
DIPiDIP-32912N.
IntActiO15514. 18 interactors.
STRINGi9606.ENSP00000272645.

Structurei

Secondary structure

1142
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 19Combined sources3
Beta strandi24 – 28Combined sources5
Helixi34 – 49Combined sources16
Helixi59 – 70Combined sources12
Helixi77 – 88Combined sources12
Helixi94 – 103Combined sources10
Helixi108 – 114Combined sources7
Helixi116 – 118Combined sources3
Turni119 – 121Combined sources3
Helixi124 – 137Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C35X-ray2.70A/C/E/G1-142[»]
5IY6electron microscopy7.20D1-142[»]
5IY7electron microscopy8.60D1-142[»]
5IY8electron microscopy7.90D1-142[»]
5IY9electron microscopy6.30D1-142[»]
5IYAelectron microscopy5.40D1-142[»]
5IYBelectron microscopy3.90D1-142[»]
5IYCelectron microscopy3.90D1-142[»]
5IYDelectron microscopy3.90D1-142[»]
ProteinModelPortaliO15514.
SMRiO15514.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15514.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2351. Eukaryota.
COG5250. LUCA.
GeneTreeiENSGT00390000004912.
HOGENOMiHOG000195281.
HOVERGENiHBG002800.
InParanoidiO15514.
KOiK03012.
OMAiQTMRKFQ.
OrthoDBiEOG091G06WT.
PhylomeDBiO15514.
TreeFamiTF103039.

Family and domain databases

InterProiView protein in InterPro
IPR010997. HRDC-like_sf.
IPR005574. RNA_pol_II_Rpb4.
IPR006590. RNA_pol_II_Rpb4_core.
PfamiView protein in Pfam
PF03874. RNA_pol_Rpb4. 1 hit.
SMARTiView protein in SMART
SM00657. RPOL4c. 1 hit.
SUPFAMiSSF47819. SSF47819. 1 hit.

Sequencei

Sequence statusi: Complete.

O15514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES
60 70 80 90 100
AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQK KLHKFELACL
110 120 130 140
ANLCPETAEE SKALIPSLEG RFEDEELQQI LDDIQTKRSF QY
Length:142
Mass (Da):16,311
Last modified:January 1, 1998 - v1
Checksum:i8A670386C433BE93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89387 Genomic DNA. Translation: AAC52056.1.
U85510 mRNA. Translation: AAC80226.1.
BC017205 mRNA. Translation: AAH17205.1.
BC093795 mRNA. Translation: AAH93795.1.
BC093797 mRNA. Translation: AAH93797.1.
CCDSiCCDS2151.1.
RefSeqiNP_004796.1. NM_004805.3.
UniGeneiHs.715348.

Genome annotation databases

EnsembliENST00000272645; ENSP00000272645; ENSG00000144231.
GeneIDi5433.
KEGGihsa:5433.
UCSCiuc002tpj.4. human.

Similar proteinsi

Entry informationi

Entry nameiRPB4_HUMAN
AccessioniPrimary (citable) accession number: O15514
Secondary accession number(s): Q52LT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families