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Reviewed, UniProtKB/Swiss-Prot O15504 (NUPL2_HUMAN)

Last modified June 16, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Nucleoporin-like 2
Alternative name(s):
    NLP-1
      Short name=hCG1
    NUP42 homolog
Gene names
Name: NUPL2
Synonyms: CG1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope. Ref.7 Ref.10

Subunit structure

Probable component of the nuclear pore complex (NPC). Interacts with nuclear export protein NXF1. Interacts with GLE1. Able to form a heterotrimer with NUP155 and GLE1 in vitro. Interacts with the HIV-1 virus proteins Rev and Vpr. The interaction with HIV-1 Rev, a protein that mediates nuclear export of unspliced viral RNAs, suggests that its function may be bypassed by the HIV-1 virus. Ref.10 Ref.6 Ref.8 Ref.9

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Excluded from the nucleolus. Ref.10 Ref.8 Ref.9

Tissue specificity

Ubiquitously expressed. Ref.8

Domain

The FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC By similarity.

Post-translational modification

O-glycosylated. Ref.8

Sequence similarities

Contains 1 C3H1-type zinc finger.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O15504-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O15504-2)

The sequence of this isoform differs from the canonical sequence as follows:
     204-224: LSDVKDGVNQAAPAFGFGSSQ → KCLWKIYTHLSPKIYGPDCAL
     225-423: Missing.
Isoform 3 (identifier: O15504-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Nucleoporin-like 2
PRO_0000204895

Regions

Repeat14 – 152FG 1
Repeat95 – 962FG 2
Repeat218 – 2192FG 3
Repeat220 – 2212FG 4
Repeat265 – 2662FG 5
Repeat271 – 2722FG 6
Repeat288 – 2892FG 7
Repeat290 – 2912FG 8
Repeat311 – 3122FG 9
Repeat336 – 3372FG 10
Repeat345 – 3462FG 11
Repeat364 – 3652FG 12
Zinc finger1 – 2525C3H1-type
Region94 – 17077Interaction with HIV-1 Vpr
Region365 – 42359Interaction with GLE1
Compositional bias238 – 378141Ser-rich

Natural variations

Alternative sequence1 – 228228Missing in isoform 3.
VSP_016480
Alternative sequence204 – 22421LSDVK…FGSSQ → KCLWKIYTHLSPKIYGPDCA L in isoform 2.
VSP_016481
Alternative sequence225 – 423199Missing in isoform 2.
VSP_016482
Natural variant3911D → N: dbSNP rs13243961.
VAR_050572
Natural variant3921K → N: dbSNP rs34902971.
VAR_050573

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 6F8B95073312E999

FASTA42344,872
        10         20         30         40         50         60 
MAICQFFLQG RCRFGDRCWN EHPGARGAGG GRQQPQQQPS GNNRRGWNTT SQRYSNVIQP 

        70         80         90        100        110        120 
SSFSKSTPWG GSRDQEKPYF SSFDSGASTN RKEGFGLSEN PFASLSPDEQ KDEKKLLEGI 

       130        140        150        160        170        180 
VKDMEVWESS GQWMFSVYSP VKKKPNISGF TDISPEELRL EYHNFLTSNN LQSYLNSVQR 

       190        200        210        220        230        240 
LINQWRNRVN ELKSLNISTK VALLSDVKDG VNQAAPAFGF GSSQAATFMS PGFPVNNSSS 

       250        260        270        280        290        300 
DNAQNFSFKT NSGFAAASSG SPAGFGSSPA FGAAASTSSG ISTSAPAFGF GKPEVTSAAS 

       310        320        330        340        350        360 
FSFKSPAASS FGSPGFSGLP ASLATGPVRA PVAPAFGGGS SVAGFGSPGS HSHTAFSKPS 

       370        380        390        400        410        420 
SDTFGNSSIS TSLSASSSII ATDNVLFTPR DKLTVEELEQ FQSKKFTLGK IPLKPPPLEL 


LNV 

« Hide

Isoform 2.

Checksum: 2EBE880F1494052D
Show »

FASTA22425,542
Isoform 3.

Checksum: 90FAD1990293952F
Show »

FASTA19519,312

References

« Hide 'large scale' references
[1]"Refined mapping of a gene for autosomal dominant progressive sensorineural hearing loss (DFNA5) to a 2-cM region, and exclusion of a candidate gene that is expressed in the cochlea."
Van Laer L., Van Camp G., van Zuylen D., Green E.D., Verstreken M., Schatteman I., Van de Heyning P., Balemans W., Coucke P., Greinwald J.H., Smith R.J.H., Huizing E., Willems P.
Eur. J. Hum. Genet. 5:397-405(1997) [PubMed: 9450185] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed: 12690205] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Testis and Urinary bladder.
[6]"The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human."
Katahira J., Straesser K., Podtelejnikov A., Mann M., Jung J.U., Hurt E.
EMBO J. 18:2593-2609(1999) [PubMed: 10228171] [Abstract]
Cited for: INTERACTION WITH NXF1.
[7]"The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p."
Strahm Y., Fahrenkrog B., Zenklusen D., Rychner E., Kantor J., Rosbach M., Stutz F.
EMBO J. 18:5761-5777(1999) [PubMed: 10610322] [Abstract]
Cited for: FUNCTION.
[8]"A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1."
Farjot G., Sergeant A., Mikaelian I.
J. Biol. Chem. 274:17309-17317(1999) [PubMed: 10358091] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH XPO1 AND HIV-1 VIRUS REV PROTEIN.
[9]"Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1."
Le Rouzic E., Mousnier A., Rustum C., Stutz F., Hallberg E., Dargemont C., Benichou S.
J. Biol. Chem. 277:45091-45098(2002) [PubMed: 12228227] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH HIV-1 VIRUS VPR PROTEIN.
[10]"Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA."
Kendirgi F., Rexer D.J., Alcazar-Roman A.R., Onishko H.M., Wente S.R.
Mol. Biol. Cell 16:4304-4315(2005) [PubMed: 16000379] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLE1.

Cross-references

Sequence databases

U97198 mRNA. Translation: AAC33794.1.
BT007409 mRNA. Translation: AAP36077.1.
AK298183 mRNA. Translation: BAG60454.1.
CH236948 Genomic DNA. Translation: EAL24260.1.
BC005327 mRNA. Translation: AAH05327.1.
BC039333 mRNA. Translation: AAH39333.1.
IPIIPI00010654.
IPI00187080.
IPI00477209.
RefSeqNP_031368.1.
UniGeneHs.408241

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteO15504.

Proteomic databases

PRIDEO15504.

Genome annotation databases

EnsemblENSG00000136243. Homo sapiens. [Contig view]
GeneID11097.
KEGGhsa:11097.

Organism-specific databases

GeneCardsGC07P023188.
H-InvDBHIX0006518.
HGNCHGNC:17010. NUPL2.
PharmGKBPA134990469.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO15504.
HOVERGENO15504.
OMAO15504. RCWNEHP.

Enzyme and pathway databases

ReactomeREACT_6185. HIV Infection.

Gene expression databases

ArrayExpressO15504.
BgeeO15504.
CleanExHS_NUPL2.
GermOnlineENSG00000136243. Homo sapiens.

Family and domain databases

InterProIPR000571. Znf_CCCH.
[Graphical view]
PfamPF00642. zf-CCCH. 1 hit.
[Graphical view]
PROSITEPS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio42186.

Entry information

Entry nameNUPL2_HUMAN
AccessionPrimary (citable) accession number: O15504
Secondary accession number(s): A4D143 expand/collapse secondary AC list , B4DP42, Q49AE7, Q9BS49
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents