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O15504

- NUPL2_HUMAN

UniProt

O15504 - NUPL2_HUMAN

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Protein
Nucleoporin-like protein 2
Gene
NUPL2, CG1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 2525C3H1-type
Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nuclear export signal receptor activity Source: ProtInc
  3. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. cytokine-mediated signaling pathway Source: Reactome
  3. glucose transport Source: Reactome
  4. hexose transport Source: Reactome
  5. mRNA transport Source: UniProtKB-KW
  6. mitotic cell cycle Source: Reactome
  7. mitotic nuclear envelope disassembly Source: Reactome
  8. protein export from nucleus Source: ProtInc
  9. regulation of glucose transport Source: Reactome
  10. small molecule metabolic process Source: Reactome
  11. transmembrane transport Source: Reactome
  12. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_115831. ISG15 antiviral mechanism.
REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
REACT_6190. Rev-mediated nuclear export of HIV RNA.
REACT_6804. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_7991. Vpr-mediated nuclear import of PICs.
REACT_9395. Nuclear import of Rev protein.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin-like protein 2
Alternative name(s):
NLP-1
NUP42 homolog
Nucleoporin hCG1
Gene namesi
Name:NUPL2
Synonyms:CG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:17010. NUPL2.

Subcellular locationi

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side
Note: Excluded from the nucleolus.3 Publications

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nuclear membrane Source: UniProtKB-SubCell
  3. nuclear pore Source: UniProtKB-SubCell
  4. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134990469.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Nucleoporin-like protein 2
PRO_0000204895Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei106 – 1061Phosphoserine1 Publication

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO15504.
PaxDbiO15504.
PRIDEiO15504.

PTM databases

PhosphoSiteiO15504.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

ArrayExpressiO15504.
BgeeiO15504.
CleanExiHS_NUPL2.
GenevestigatoriO15504.

Organism-specific databases

HPAiHPA049036.
HPA057251.

Interactioni

Subunit structurei

Probable component of the nuclear pore complex (NPC). Interacts with nuclear export protein NXF1. Interacts with GLE1. Able to form a heterotrimer with NUP155 and GLE1 in vitro. Interacts with the HIV-1 virus proteins Rev and Vpr. The interaction with HIV-1 Rev, a protein that mediates nuclear export of unspliced viral RNAs, suggests that its function may be bypassed by the HIV-1 virus.4 Publications

Protein-protein interaction databases

BioGridi116278. 9 interactions.
IntActiO15504. 6 interactions.
MINTiMINT-4786853.
STRINGi9606.ENSP00000258742.

Structurei

3D structure databases

ProteinModelPortaliO15504.
SMRiO15504. Positions 1-26.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 152FG 1
Repeati95 – 962FG 2
Repeati218 – 2192FG 3
Repeati220 – 2212FG 4
Repeati265 – 2662FG 5
Repeati271 – 2722FG 6
Repeati288 – 2892FG 7
Repeati290 – 2912FG 8
Repeati311 – 3122FG 9
Repeati336 – 3372FG 10
Repeati345 – 3462FG 11
Repeati364 – 3652FG 12

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 17077Interaction with HIV-1 Vpr
Add
BLAST
Regioni365 – 42359Interaction with GLE1
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi238 – 378141Ser-rich
Add
BLAST

Domaini

The FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG69917.
HOGENOMiHOG000113895.
HOVERGENiHBG082070.
InParanoidiO15504.
KOiK14321.
OrthoDBiEOG70ZZQ7.
PhylomeDBiO15504.
TreeFamiTF106503.

Family and domain databases

InterProiIPR000571. Znf_CCCH.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O15504-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAICQFFLQG RCRFGDRCWN EHPGARGAGG GRQQPQQQPS GNNRRGWNTT    50
SQRYSNVIQP SSFSKSTPWG GSRDQEKPYF SSFDSGASTN RKEGFGLSEN 100
PFASLSPDEQ KDEKKLLEGI VKDMEVWESS GQWMFSVYSP VKKKPNISGF 150
TDISPEELRL EYHNFLTSNN LQSYLNSVQR LINQWRNRVN ELKSLNISTK 200
VALLSDVKDG VNQAAPAFGF GSSQAATFMS PGFPVNNSSS DNAQNFSFKT 250
NSGFAAASSG SPAGFGSSPA FGAAASTSSG ISTSAPAFGF GKPEVTSAAS 300
FSFKSPAASS FGSPGFSGLP ASLATGPVRA PVAPAFGGGS SVAGFGSPGS 350
HSHTAFSKPS SDTFGNSSIS TSLSASSSII ATDNVLFTPR DKLTVEELEQ 400
FQSKKFTLGK IPLKPPPLEL LNV 423
Length:423
Mass (Da):44,872
Last modified:January 1, 1998 - v1
Checksum:i6F8B95073312E999
GO
Isoform 2 (identifier: O15504-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-224: LSDVKDGVNQAAPAFGFGSSQ → KCLWKIYTHLSPKIYGPDCAL
     225-423: Missing.

Show »
Length:224
Mass (Da):25,542
Checksum:i2EBE880F1494052D
GO
Isoform 3 (identifier: O15504-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.

Show »
Length:195
Mass (Da):19,312
Checksum:i90FAD1990293952F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti391 – 3911D → N.
Corresponds to variant rs13243961 [ dbSNP | Ensembl ].
VAR_050572
Natural varianti392 – 3921K → N.
Corresponds to variant rs34902971 [ dbSNP | Ensembl ].
VAR_050573

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 228228Missing in isoform 3.
VSP_016480Add
BLAST
Alternative sequencei204 – 22421LSDVK…FGSSQ → KCLWKIYTHLSPKIYGPDCA L in isoform 2.
VSP_016481Add
BLAST
Alternative sequencei225 – 423199Missing in isoform 2.
VSP_016482Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97198 mRNA. Translation: AAC33794.1.
BT007409 mRNA. Translation: AAP36077.1.
AK298183 mRNA. Translation: BAG60454.1.
CH236948 Genomic DNA. Translation: EAL24260.1.
BC005327 mRNA. Translation: AAH05327.1.
BC039333 mRNA. Translation: AAH39333.1.
CCDSiCCDS5379.1. [O15504-1]
RefSeqiNP_031368.1. NM_007342.2. [O15504-1]
UniGeneiHs.408241.

Genome annotation databases

EnsembliENST00000258742; ENSP00000258742; ENSG00000136243. [O15504-1]
ENST00000438012; ENSP00000415511; ENSG00000136243. [O15504-2]
GeneIDi11097.
KEGGihsa:11097.
UCSCiuc003svu.3. human. [O15504-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U97198 mRNA. Translation: AAC33794.1 .
BT007409 mRNA. Translation: AAP36077.1 .
AK298183 mRNA. Translation: BAG60454.1 .
CH236948 Genomic DNA. Translation: EAL24260.1 .
BC005327 mRNA. Translation: AAH05327.1 .
BC039333 mRNA. Translation: AAH39333.1 .
CCDSi CCDS5379.1. [O15504-1 ]
RefSeqi NP_031368.1. NM_007342.2. [O15504-1 ]
UniGenei Hs.408241.

3D structure databases

ProteinModelPortali O15504.
SMRi O15504. Positions 1-26.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116278. 9 interactions.
IntActi O15504. 6 interactions.
MINTi MINT-4786853.
STRINGi 9606.ENSP00000258742.

PTM databases

PhosphoSitei O15504.

Proteomic databases

MaxQBi O15504.
PaxDbi O15504.
PRIDEi O15504.

Protocols and materials databases

DNASUi 11097.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000258742 ; ENSP00000258742 ; ENSG00000136243 . [O15504-1 ]
ENST00000438012 ; ENSP00000415511 ; ENSG00000136243 . [O15504-2 ]
GeneIDi 11097.
KEGGi hsa:11097.
UCSCi uc003svu.3. human. [O15504-1 ]

Organism-specific databases

CTDi 11097.
GeneCardsi GC07P023222.
HGNCi HGNC:17010. NUPL2.
HPAi HPA049036.
HPA057251.
neXtProti NX_O15504.
PharmGKBi PA134990469.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG69917.
HOGENOMi HOG000113895.
HOVERGENi HBG082070.
InParanoidi O15504.
KOi K14321.
OrthoDBi EOG70ZZQ7.
PhylomeDBi O15504.
TreeFami TF106503.

Enzyme and pathway databases

Reactomei REACT_115831. ISG15 antiviral mechanism.
REACT_163931. Nuclear Pore Complex (NPC) Disassembly.
REACT_6190. Rev-mediated nuclear export of HIV RNA.
REACT_6804. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_7991. Vpr-mediated nuclear import of PICs.
REACT_9395. Nuclear import of Rev protein.

Miscellaneous databases

ChiTaRSi NUPL2. human.
GeneWikii NUPL2.
GenomeRNAii 11097.
NextBioi 42186.
PROi O15504.

Gene expression databases

ArrayExpressi O15504.
Bgeei O15504.
CleanExi HS_NUPL2.
Genevestigatori O15504.

Family and domain databases

InterProi IPR000571. Znf_CCCH.
[Graphical view ]
PROSITEi PS50103. ZF_C3H1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Refined mapping of a gene for autosomal dominant progressive sensorineural hearing loss (DFNA5) to a 2-cM region, and exclusion of a candidate gene that is expressed in the cochlea."
    Van Laer L., Van Camp G., van Zuylen D., Green E.D., Verstreken M., Schatteman I., Van de Heyning P., Balemans W., Coucke P., Greinwald J.H., Smith R.J.H., Huizing E., Willems P.
    Eur. J. Hum. Genet. 5:397-405(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  4. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Testis and Urinary bladder.
  6. "The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human."
    Katahira J., Straesser K., Podtelejnikov A., Mann M., Jung J.U., Hurt E.
    EMBO J. 18:2593-2609(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NXF1.
  7. "The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p."
    Strahm Y., Fahrenkrog B., Zenklusen D., Rychner E., Kantor J., Rosbach M., Stutz F.
    EMBO J. 18:5761-5777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1."
    Farjot G., Sergeant A., Mikaelian I.
    J. Biol. Chem. 274:17309-17317(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH XPO1 AND HIV-1 VIRUS REV PROTEIN.
  9. "Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1."
    Le Rouzic E., Mousnier A., Rustum C., Stutz F., Hallberg E., Dargemont C., Benichou S.
    J. Biol. Chem. 277:45091-45098(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH HIV-1 VIRUS VPR PROTEIN.
  10. "Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA."
    Kendirgi F., Rexer D.J., Alcazar-Roman A.R., Onishko H.M., Wente S.R.
    Mol. Biol. Cell 16:4304-4315(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLE1.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiNUPL2_HUMAN
AccessioniPrimary (citable) accession number: O15504
Secondary accession number(s): A4D143
, B4DP42, Q49AE7, Q9BS49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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