Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoporin-like protein 2

Gene

NUPL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 2525C3H1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • nuclear export signal receptor activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin-like protein 2
Alternative name(s):
NLP-1
NUP42 homolog
Nucleoporin hCG1
Gene namesi
Name:NUPL2
Synonyms:CG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:17010. NUPL2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134990469.

Polymorphism and mutation databases

BioMutaiNUPL2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Nucleoporin-like protein 2PRO_0000204895Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei106 – 1061PhosphoserineCombined sources

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO15504.
MaxQBiO15504.
PaxDbiO15504.
PeptideAtlasiO15504.
PRIDEiO15504.

PTM databases

iPTMnetiO15504.
PhosphoSiteiO15504.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000136243.
CleanExiHS_NUPL2.
ExpressionAtlasiO15504. baseline and differential.
GenevisibleiO15504. HS.

Organism-specific databases

HPAiHPA049036.
HPA057251.

Interactioni

Subunit structurei

Probable component of the nuclear pore complex (NPC). Interacts with nuclear export protein NXF1. Interacts with GLE1. Able to form a heterotrimer with NUP155 and GLE1 in vitro. Interacts with the HIV-1 virus proteins Rev and Vpr. The interaction with HIV-1 Rev, a protein that mediates nuclear export of unspliced viral RNAs, suggests that its function may be bypassed by the HIV-1 virus.4 Publications

Protein-protein interaction databases

BioGridi116278. 22 interactions.
IntActiO15504. 17 interactions.
MINTiMINT-4786853.
STRINGi9606.ENSP00000258742.

Structurei

3D structure databases

ProteinModelPortaliO15504.
SMRiO15504. Positions 1-26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 152FG 1
Repeati95 – 962FG 2
Repeati218 – 2192FG 3
Repeati220 – 2212FG 4
Repeati265 – 2662FG 5
Repeati271 – 2722FG 6
Repeati288 – 2892FG 7
Repeati290 – 2912FG 8
Repeati311 – 3122FG 9
Repeati336 – 3372FG 10
Repeati345 – 3462FG 11
Repeati364 – 3652FG 12

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 17077Interaction with HIV-1 VprAdd
BLAST
Regioni365 – 42359Interaction with GLE1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi238 – 378141Ser-richAdd
BLAST

Domaini

The FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC.By similarity

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1 – 2525C3H1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIH3. Eukaryota.
ENOG4111HKJ. LUCA.
GeneTreeiENSGT00390000000118.
HOGENOMiHOG000113895.
HOVERGENiHBG082070.
InParanoidiO15504.
KOiK14321.
PhylomeDBiO15504.
TreeFamiTF106503.

Family and domain databases

InterProiIPR000571. Znf_CCCH.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 1 hit.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15504-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAICQFFLQG RCRFGDRCWN EHPGARGAGG GRQQPQQQPS GNNRRGWNTT
60 70 80 90 100
SQRYSNVIQP SSFSKSTPWG GSRDQEKPYF SSFDSGASTN RKEGFGLSEN
110 120 130 140 150
PFASLSPDEQ KDEKKLLEGI VKDMEVWESS GQWMFSVYSP VKKKPNISGF
160 170 180 190 200
TDISPEELRL EYHNFLTSNN LQSYLNSVQR LINQWRNRVN ELKSLNISTK
210 220 230 240 250
VALLSDVKDG VNQAAPAFGF GSSQAATFMS PGFPVNNSSS DNAQNFSFKT
260 270 280 290 300
NSGFAAASSG SPAGFGSSPA FGAAASTSSG ISTSAPAFGF GKPEVTSAAS
310 320 330 340 350
FSFKSPAASS FGSPGFSGLP ASLATGPVRA PVAPAFGGGS SVAGFGSPGS
360 370 380 390 400
HSHTAFSKPS SDTFGNSSIS TSLSASSSII ATDNVLFTPR DKLTVEELEQ
410 420
FQSKKFTLGK IPLKPPPLEL LNV
Length:423
Mass (Da):44,872
Last modified:January 1, 1998 - v1
Checksum:i6F8B95073312E999
GO
Isoform 2 (identifier: O15504-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-224: LSDVKDGVNQAAPAFGFGSSQ → KCLWKIYTHLSPKIYGPDCAL
     225-423: Missing.

Show »
Length:224
Mass (Da):25,542
Checksum:i2EBE880F1494052D
GO
Isoform 3 (identifier: O15504-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.

Show »
Length:195
Mass (Da):19,312
Checksum:i90FAD1990293952F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti391 – 3911D → N.
Corresponds to variant rs13243961 [ dbSNP | Ensembl ].
VAR_050572
Natural varianti392 – 3921K → N.
Corresponds to variant rs34902971 [ dbSNP | Ensembl ].
VAR_050573

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 228228Missing in isoform 3. 2 PublicationsVSP_016480Add
BLAST
Alternative sequencei204 – 22421LSDVK…FGSSQ → KCLWKIYTHLSPKIYGPDCA L in isoform 2. 2 PublicationsVSP_016481Add
BLAST
Alternative sequencei225 – 423199Missing in isoform 2. 2 PublicationsVSP_016482Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97198 mRNA. Translation: AAC33794.1.
BT007409 mRNA. Translation: AAP36077.1.
AK298183 mRNA. Translation: BAG60454.1.
CH236948 Genomic DNA. Translation: EAL24260.1.
BC005327 mRNA. Translation: AAH05327.1.
BC039333 mRNA. Translation: AAH39333.1.
CCDSiCCDS5379.1. [O15504-1]
RefSeqiNP_031368.1. NM_007342.2. [O15504-1]
UniGeneiHs.408241.

Genome annotation databases

EnsembliENST00000258742; ENSP00000258742; ENSG00000136243. [O15504-1]
ENST00000438012; ENSP00000415511; ENSG00000136243. [O15504-2]
GeneIDi11097.
KEGGihsa:11097.
UCSCiuc003svu.4. human. [O15504-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97198 mRNA. Translation: AAC33794.1.
BT007409 mRNA. Translation: AAP36077.1.
AK298183 mRNA. Translation: BAG60454.1.
CH236948 Genomic DNA. Translation: EAL24260.1.
BC005327 mRNA. Translation: AAH05327.1.
BC039333 mRNA. Translation: AAH39333.1.
CCDSiCCDS5379.1. [O15504-1]
RefSeqiNP_031368.1. NM_007342.2. [O15504-1]
UniGeneiHs.408241.

3D structure databases

ProteinModelPortaliO15504.
SMRiO15504. Positions 1-26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116278. 22 interactions.
IntActiO15504. 17 interactions.
MINTiMINT-4786853.
STRINGi9606.ENSP00000258742.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiO15504.
PhosphoSiteiO15504.

Polymorphism and mutation databases

BioMutaiNUPL2.

Proteomic databases

EPDiO15504.
MaxQBiO15504.
PaxDbiO15504.
PeptideAtlasiO15504.
PRIDEiO15504.

Protocols and materials databases

DNASUi11097.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258742; ENSP00000258742; ENSG00000136243. [O15504-1]
ENST00000438012; ENSP00000415511; ENSG00000136243. [O15504-2]
GeneIDi11097.
KEGGihsa:11097.
UCSCiuc003svu.4. human. [O15504-1]

Organism-specific databases

CTDi11097.
GeneCardsiNUPL2.
HGNCiHGNC:17010. NUPL2.
HPAiHPA049036.
HPA057251.
neXtProtiNX_O15504.
PharmGKBiPA134990469.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIH3. Eukaryota.
ENOG4111HKJ. LUCA.
GeneTreeiENSGT00390000000118.
HOGENOMiHOG000113895.
HOVERGENiHBG082070.
InParanoidiO15504.
KOiK14321.
PhylomeDBiO15504.
TreeFamiTF106503.

Enzyme and pathway databases

ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-165054. Rev-mediated nuclear export of HIV RNA.
R-HSA-168276. NS1 Mediated Effects on Host Pathways.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-HSA-180746. Nuclear import of Rev protein.
R-HSA-180910. Vpr-mediated nuclear import of PICs.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-4570464. SUMOylation of RNA binding proteins.
R-HSA-4615885. SUMOylation of DNA replication proteins.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-6784531. tRNA processing in the nucleus.

Miscellaneous databases

ChiTaRSiNUPL2. human.
GeneWikiiNUPL2.
GenomeRNAii11097.
PROiO15504.

Gene expression databases

BgeeiENSG00000136243.
CleanExiHS_NUPL2.
ExpressionAtlasiO15504. baseline and differential.
GenevisibleiO15504. HS.

Family and domain databases

InterProiIPR000571. Znf_CCCH.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 1 hit.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUPL2_HUMAN
AccessioniPrimary (citable) accession number: O15504
Secondary accession number(s): A4D143
, B4DP42, Q49AE7, Q9BS49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.