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O15504 (NUPL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin-like protein 2
Alternative name(s):
NLP-1
NUP42 homolog
Nucleoporin hCG1
Gene names
Name:NUPL2
Synonyms:CG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. In case of infection by HIV-1, it may participate in the docking of viral Vpr at the nuclear envelope. Ref.7 Ref.10

Subunit structure

Probable component of the nuclear pore complex (NPC). Interacts with nuclear export protein NXF1. Interacts with GLE1. Able to form a heterotrimer with NUP155 and GLE1 in vitro. Interacts with the HIV-1 virus proteins Rev and Vpr. The interaction with HIV-1 Rev, a protein that mediates nuclear export of unspliced viral RNAs, suggests that its function may be bypassed by the HIV-1 virus. Ref.6 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Excluded from the nucleolus. Ref.8 Ref.9 Ref.10

Tissue specificity

Ubiquitously expressed. Ref.8

Domain

The FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC By similarity.

Post-translational modification

O-glycosylated. Ref.8

Sequence similarities

Contains 1 C3H1-type zinc finger.

Ontologies

Keywords
   Biological processmRNA transport
Protein transport
Translocation
Transport
   Cellular componentMembrane
Nuclear pore complex
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

glucose transport

Traceable author statement. Source: Reactome

hexose transport

Traceable author statement. Source: Reactome

mRNA transport

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic cell cycle

Traceable author statement. Source: Reactome

mitotic nuclear envelope disassembly

Traceable author statement. Source: Reactome

protein export from nucleus

Traceable author statement Ref.8. Source: ProtInc

regulation of glucose transport

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

nuclear membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear pore

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear export signal receptor activity

Traceable author statement Ref.8. Source: ProtInc

poly(A) RNA binding

Inferred from direct assay PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O15504-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O15504-2)

The sequence of this isoform differs from the canonical sequence as follows:
     204-224: LSDVKDGVNQAAPAFGFGSSQ → KCLWKIYTHLSPKIYGPDCAL
     225-423: Missing.
Isoform 3 (identifier: O15504-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Nucleoporin-like protein 2
PRO_0000204895

Regions

Repeat14 – 152FG 1
Repeat95 – 962FG 2
Repeat218 – 2192FG 3
Repeat220 – 2212FG 4
Repeat265 – 2662FG 5
Repeat271 – 2722FG 6
Repeat288 – 2892FG 7
Repeat290 – 2912FG 8
Repeat311 – 3122FG 9
Repeat336 – 3372FG 10
Repeat345 – 3462FG 11
Repeat364 – 3652FG 12
Zinc finger1 – 2525C3H1-type
Region94 – 17077Interaction with HIV-1 Vpr
Region365 – 42359Interaction with GLE1
Compositional bias238 – 378141Ser-rich

Amino acid modifications

Modified residue1061Phosphoserine Ref.12

Natural variations

Alternative sequence1 – 228228Missing in isoform 3.
VSP_016480
Alternative sequence204 – 22421LSDVK…FGSSQ → KCLWKIYTHLSPKIYGPDCA L in isoform 2.
VSP_016481
Alternative sequence225 – 423199Missing in isoform 2.
VSP_016482
Natural variant3911D → N.
Corresponds to variant rs13243961 [ dbSNP | Ensembl ].
VAR_050572
Natural variant3921K → N.
Corresponds to variant rs34902971 [ dbSNP | Ensembl ].
VAR_050573

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 6F8B95073312E999

FASTA42344,872
        10         20         30         40         50         60 
MAICQFFLQG RCRFGDRCWN EHPGARGAGG GRQQPQQQPS GNNRRGWNTT SQRYSNVIQP 

        70         80         90        100        110        120 
SSFSKSTPWG GSRDQEKPYF SSFDSGASTN RKEGFGLSEN PFASLSPDEQ KDEKKLLEGI 

       130        140        150        160        170        180 
VKDMEVWESS GQWMFSVYSP VKKKPNISGF TDISPEELRL EYHNFLTSNN LQSYLNSVQR 

       190        200        210        220        230        240 
LINQWRNRVN ELKSLNISTK VALLSDVKDG VNQAAPAFGF GSSQAATFMS PGFPVNNSSS 

       250        260        270        280        290        300 
DNAQNFSFKT NSGFAAASSG SPAGFGSSPA FGAAASTSSG ISTSAPAFGF GKPEVTSAAS 

       310        320        330        340        350        360 
FSFKSPAASS FGSPGFSGLP ASLATGPVRA PVAPAFGGGS SVAGFGSPGS HSHTAFSKPS 

       370        380        390        400        410        420 
SDTFGNSSIS TSLSASSSII ATDNVLFTPR DKLTVEELEQ FQSKKFTLGK IPLKPPPLEL 


LNV 

« Hide

Isoform 2 [UniParc].

Checksum: 2EBE880F1494052D
Show »

FASTA22425,542
Isoform 3 [UniParc].

Checksum: 90FAD1990293952F
Show »

FASTA19519,312

References

« Hide 'large scale' references
[1]"Refined mapping of a gene for autosomal dominant progressive sensorineural hearing loss (DFNA5) to a 2-cM region, and exclusion of a candidate gene that is expressed in the cochlea."
Van Laer L., Van Camp G., van Zuylen D., Green E.D., Verstreken M., Schatteman I., Van de Heyning P., Balemans W., Coucke P., Greinwald J.H., Smith R.J.H., Huizing E., Willems P.
Eur. J. Hum. Genet. 5:397-405(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Testis and Urinary bladder.
[6]"The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human."
Katahira J., Straesser K., Podtelejnikov A., Mann M., Jung J.U., Hurt E.
EMBO J. 18:2593-2609(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NXF1.
[7]"The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p."
Strahm Y., Fahrenkrog B., Zenklusen D., Rychner E., Kantor J., Rosbach M., Stutz F.
EMBO J. 18:5761-5777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1."
Farjot G., Sergeant A., Mikaelian I.
J. Biol. Chem. 274:17309-17317(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, TISSUE SPECIFICITY, INTERACTION WITH XPO1 AND HIV-1 VIRUS REV PROTEIN.
[9]"Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1."
Le Rouzic E., Mousnier A., Rustum C., Stutz F., Hallberg E., Dargemont C., Benichou S.
J. Biol. Chem. 277:45091-45098(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH HIV-1 VIRUS VPR PROTEIN.
[10]"Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA."
Kendirgi F., Rexer D.J., Alcazar-Roman A.R., Onishko H.M., Wente S.R.
Mol. Biol. Cell 16:4304-4315(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLE1.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U97198 mRNA. Translation: AAC33794.1.
BT007409 mRNA. Translation: AAP36077.1.
AK298183 mRNA. Translation: BAG60454.1.
CH236948 Genomic DNA. Translation: EAL24260.1.
BC005327 mRNA. Translation: AAH05327.1.
BC039333 mRNA. Translation: AAH39333.1.
CCDSCCDS5379.1. [O15504-1]
RefSeqNP_031368.1. NM_007342.2. [O15504-1]
UniGeneHs.408241.

3D structure databases

ProteinModelPortalO15504.
SMRO15504. Positions 1-26.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116278. 9 interactions.
IntActO15504. 6 interactions.
MINTMINT-4786853.
STRING9606.ENSP00000258742.

PTM databases

PhosphoSiteO15504.

Proteomic databases

MaxQBO15504.
PaxDbO15504.
PRIDEO15504.

Protocols and materials databases

DNASU11097.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000258742; ENSP00000258742; ENSG00000136243. [O15504-1]
ENST00000438012; ENSP00000415511; ENSG00000136243. [O15504-2]
GeneID11097.
KEGGhsa:11097.
UCSCuc003svu.3. human. [O15504-1]

Organism-specific databases

CTD11097.
GeneCardsGC07P023222.
HGNCHGNC:17010. NUPL2.
HPAHPA049036.
HPA057251.
neXtProtNX_O15504.
PharmGKBPA134990469.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG69917.
HOGENOMHOG000113895.
HOVERGENHBG082070.
InParanoidO15504.
KOK14321.
OrthoDBEOG70ZZQ7.
PhylomeDBO15504.
TreeFamTF106503.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_115566. Cell Cycle.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.
REACT_21300. Mitotic M-M/G1 phases.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressO15504.
BgeeO15504.
CleanExHS_NUPL2.
GenevestigatorO15504.

Family and domain databases

InterProIPR000571. Znf_CCCH.
[Graphical view]
PROSITEPS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUPL2. human.
GeneWikiNUPL2.
GenomeRNAi11097.
NextBio42186.
PROO15504.

Entry information

Entry nameNUPL2_HUMAN
AccessionPrimary (citable) accession number: O15504
Secondary accession number(s): A4D143 expand/collapse secondary AC list , B4DP42, Q49AE7, Q9BS49
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM