##gff-version 3
O15503	UniProtKB	Chain	1	277	.	.	.	ID=PRO_0000191675;Note=Insulin-induced gene 1 protein	
O15503	UniProtKB	Topological domain	1	84	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14660594;Dbxref=PMID:14660594	
O15503	UniProtKB	Transmembrane	85	107	.	.	.	Note=Helical%3B Name%3D1;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	108	126	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Transmembrane	127	144	.	.	.	Note=Helical%3B Name%3D2;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	145	159	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Transmembrane	160	182	.	.	.	Note=Helical%3B Name%3D3;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	183	185	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Transmembrane	186	204	.	.	.	Note=Helical%3B Name%3D4;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	205	209	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14660594;Dbxref=PMID:14660594	
O15503	UniProtKB	Transmembrane	210	231	.	.	.	Note=Helical%3B Name%3D5;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	232	245	.	.	.	Note=Lumenal;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Transmembrane	246	263	.	.	.	Note=Helical%3B Name%3D6;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:A1T557	
O15503	UniProtKB	Topological domain	264	277	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:14660594;Dbxref=PMID:14660594	
O15503	UniProtKB	Region	51	73	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
O15503	UniProtKB	Motif	271	277	.	.	.	Note=KxHxx;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:23481256;Dbxref=PMID:23481256	
O15503	UniProtKB	Site	171	171	.	.	.	Note=Required for the recognition of 25-hydroxycholesterol;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9Y5U4	
O15503	UniProtKB	Modified residue	207	207	.	.	.	Note=Phosphoserine%3B by PCK1;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
O15503	UniProtKB	Cross-link	156	156	.	.	.	Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16399501;Dbxref=PMID:16399501	
O15503	UniProtKB	Cross-link	158	158	.	.	.	Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16399501;Dbxref=PMID:16399501	
O15503	UniProtKB	Alternative sequence	138	164	.	.	.	ID=VSP_045084;Note=In isoform 2. AVVGLLYPCIDSHLGEPHKFKREWASV->GIHPQISSIFVLGSLVYFSQEASRWGT;Ontology_term=ECO:0000303;evidence=ECO:0000303|Ref.5	
O15503	UniProtKB	Alternative sequence	165	277	.	.	.	ID=VSP_045085;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|Ref.5	
O15503	UniProtKB	Natural variant	27	27	.	.	.	ID=VAR_027683;Note=A->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9268630;Dbxref=dbSNP:rs1129825,PMID:9268630	
O15503	UniProtKB	Mutagenesis	156	156	.	.	.	Note=Loss of ubiquitination and degradation. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16399501;Dbxref=PMID:16399501	
O15503	UniProtKB	Mutagenesis	158	158	.	.	.	Note=Loss of ubiquitination and degradation. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16399501;Dbxref=PMID:16399501	
O15503	UniProtKB	Mutagenesis	205	205	.	.	.	Note=Loss of ability to suppress the cleavage of SREBP2 and to accelerate the degradation of HMGCR. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16606821;Dbxref=PMID:16606821	
O15503	UniProtKB	Mutagenesis	207	207	.	.	.	Note=Abolished phosphorylation by PCK1%2C does not affect oxysterol-binding%2C does not affect the interaction with SCAP. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
O15503	UniProtKB	Mutagenesis	207	207	.	.	.	Note=Phosphomimetic mutant%2C reduced binding to oxysterol. S->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32322062;Dbxref=PMID:32322062	
O15503	UniProtKB	Sequence conflict	31	32	.	.	.	Note=AA->PP;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Sequence conflict	99	99	.	.	.	Note=A->T;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O15503	UniProtKB	Sequence conflict	170	172	.	.	.	Note=VFV->GFG;Ontology_term=ECO:0000305;evidence=ECO:0000305