O15498 (YKT6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptobrevin homolog YKT6
EC=2.3.1.-

Gene names

Name:

YKT6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity. Ref.1 Ref.7
Subunit structure	Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5 By similarity.
Subcellular location	Cytoplasm › cytosol. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Note: Probably cycles through vesicles between Golgi and endosomes. Ref.7 Ref.8
Domain	The longin domain regulates palmitoylation and membrane targeting.
Post-translational modification	Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting. Ref.6 Ref.8 Farnesylation is required for Golgi targeting.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Coiled coil
Molecular function	Transferase
PTM	Lipoprotein Methylation Palmitate Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from direct assay Ref.1. Source: HGNC protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from direct assay Ref.7. Source: HGNC vesicle docking involved in exocytosis Inferred from direct assay Ref.1. Source: HGNC vesicle targeting Inferred from direct assay Ref.1. Source: HGNC
Cellular_component	Golgi apparatus Inferred from direct assay Ref.7. Source: HGNC Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Inferred from sequence or structural similarity. Source: HGNC cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay Ref.1. Source: HGNC endosome Inferred from direct assay Ref.7. Source: HGNC integral to plasma membrane Inferred from direct assay Ref.1. Source: HGNC mitochondrion Inferred from direct assay. Source: HPA
Molecular_function	SNAP receptor activity Inferred from direct assay Ref.7. Source: HGNC protein-cysteine S-palmitoleyltransferase activity Inferred from direct assay Ref.6. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Synaptobrevin homolog YKT6		PRO_0000280709
	Propeptide	196 – 198	3	Removed in mature form Probable		PRO_0000396661
Regions
	Domain	8 – 126	119	Longin
	Domain	138 – 198	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	195	1	Cysteine methyl ester Probable
	Lipidation	194	1	S-palmitoyl cysteine Ref.6 Ref.8
	Lipidation	195	1	S-farnesyl cysteine Ref.8
Experimental info
	Mutagenesis	42	1	F → E: Increases palmitoylation. Targeted to Golgi membranes. Targeted to Golgi and cytosol; when associated with S-194. Targeted to cytosol; when associated with S-195. Ref.6 Ref.8
	Mutagenesis	194	1	C → S: Decreases palmitoylation by 55%. Prevents palmitoylation; when associated with S-195. Targeted to Golgi and cytosol; when associated with E-42. Ref.6 Ref.8
	Mutagenesis	195	1	C → S: Prevents farnesylation. Targeted to cytosol; when associated with E-42. Decreases palmitoylation by 13%. Prevents palmitoylation; when associated with S-194. Ref.6 Ref.8
	Sequence conflict	63	1	D → N in CAG46805. Ref.3
	Sequence conflict	138	1	P → L in BAD97208. Ref.4
	Sequence conflict	198	1	M → I in CAG33270. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O15498 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2AB0C12832D1B943	FASTA	198	22,418
	10 20 30 40 50 60 MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER SSKGTRASVK 70 80 90 100 110 120 EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE FSKQVDRIDW PVGSPATIHY 130 140 150 160 170 180 PALDGHLSRY QNPREADPMT KVQAELDETK IILHNTMESL LERGEKLDDL VSKSEVLGTQ 190 SKAFYKTARK QNSCCAIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport." McNew J.A., Soegaard M., Lampen N.M., Machida S., Ye R.R., Lacomis L., Tempst P., Rothman J.E., Soellner T.H. J. Biol. Chem. 272:17776-17783(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Pancreas.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hepatoma.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[6]	"The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues." Veit M. Biochem. J. 384:233-237(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, PALMITOYLATION AT CYS-194.
[7]	"Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network." Tai G., Lu L., Wang T.L., Tang B.L., Goud B., Johannes L., Hong W. Mol. Biol. Cell 15:4011-4022(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation." Fukasawa M., Varlamov O., Eng W.S., Soellner T.H., Rothman J.E. Proc. Natl. Acad. Sci. U.S.A. 101:4815-4820(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, ISOPRENYLATION AT CYS-195, PALMITOYLATION.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U95735 mRNA. Translation: AAB81131.1. BT007078 mRNA. Translation: AAP35741.1. CR456989 mRNA. Translation: CAG33270.1. CR542008 mRNA. Translation: CAG46805.1. AK223488 mRNA. Translation: BAD97208.1. BC007319 mRNA. Translation: AAH07319.1.
IPI	IPI00008569.
RefSeq	NP_006546.1. NM_006555.3.
UniGene	Hs.520794.
3D structure databases
HSSP	HSSP built from PDB template 1IOU based on UniProtKB P36015.
ProteinModelPortal	O15498.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000223369.
PTM databases
PhosphoSite	O15498.
Proteomic databases
PaxDb	O15498.
PRIDE	O15498.
Protocols and materials databases
DNASU	10652.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000223369; ENSP00000223369; ENSG00000106636.
GeneID	10652.
KEGG	hsa:10652.
UCSC	uc003tkm.3. human.
Organism-specific databases
CTD	10652.
GeneCards	GC07P044207.
HGNC	HGNC:16959. YKT6.
HPA	HPA030817. HPA030818.
MIM	606209. gene.
neXtProt	NX_O15498.
PharmGKB	PA145007308.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5143.
HOGENOM	HOG000189970.
HOVERGEN	HBG088939.
InParanoid	O15498.
KO	K08516.
OMA	KQNSCCE.
OrthoDB	EOG4FFD2W.
PhylomeDB	O15498.
Gene expression databases
ArrayExpress	O15498.
Bgee	O15498.
CleanEx	HS_YKT6.
Genevestigator	O15498.
Family and domain databases
Gene3D	3.30.450.50. 1 hit.
InterPro	IPR011012. Longin-like_dom. IPR010908. Longin_dom. IPR001388. Synaptobrevin. [Graphical view]
Pfam	PF13774. Longin. 1 hit. PF00957. Synaptobrevin. 1 hit. [Graphical view]
SUPFAM	SSF64356. Longin_like. 1 hit.
PROSITE	PS50859. LONGIN. 1 hit. PS00417. SYNAPTOBREVIN. False negative. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10652.
NextBio	40495.
SOURCE	Search...

Entry information

Entry name

YKT6_HUMAN

Accession

Primary (citable) accession number: O15498
Secondary accession number(s): Q53F01, Q6FGU9, Q6IB15

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.