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O15498 (YKT6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptobrevin homolog YKT6

EC=2.3.1.-
Gene names
Name:YKT6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity. Ref.1 Ref.8

Subunit structure

Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5 By similarity.

Subcellular location

Cytoplasmcytosol. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Note: Probably cycles through vesicles between Golgi and endosomes. Ref.7 Ref.8 Ref.9

Domain

The longin domain regulates palmitoylation and membrane targeting.

Post-translational modification

Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting. Ref.6 Ref.9

Farnesylation is required for Golgi targeting.

Sequence similarities

Belongs to the synaptobrevin family.

Contains 1 longin domain.

Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Biological processER-Golgi transport
Protein transport
Transport
   Cellular componentCytoplasm
Cytoplasmic vesicle
Golgi apparatus
Membrane
   DomainCoiled coil
   Molecular functionTransferase
   PTMLipoprotein
Methylation
Palmitate
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processER to Golgi vesicle-mediated transport

Inferred from direct assay Ref.1. Source: HGNC

metabolic process

Inferred from direct assay Ref.6. Source: GOC

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

retrograde transport, endosome to Golgi

Inferred from direct assay Ref.8. Source: HGNC

vesicle docking involved in exocytosis

Inferred from direct assay Ref.1. Source: HGNC

vesicle fusion

Inferred from Biological aspect of Ancestor. Source: RefGenome

vesicle targeting

Inferred from direct assay Ref.1. Source: HGNC

   Cellular_componentGolgi apparatus

Inferred from direct assay Ref.8. Source: HGNC

Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

SNARE complex

Inferred from sequence or structural similarity. Source: HGNC

cytoplasm

Inferred from direct assay. Source: HPA

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum

Inferred from direct assay Ref.1. Source: HGNC

endosome

Inferred from direct assay Ref.8. Source: HGNC

integral component of plasma membrane

Inferred from direct assay Ref.1. Source: HGNC

mitochondrion

Inferred from direct assay. Source: HPA

   Molecular_functionSNAP receptor activity

Inferred from direct assay Ref.8. Source: HGNC

SNARE binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein-cysteine S-palmitoyltransferase activity

Inferred from direct assay Ref.6. Source: HGNC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Synaptobrevin homolog YKT6
PRO_0000280709
Propeptide196 – 1983Removed in mature form Probable
PRO_0000396661

Regions

Domain8 – 126119Longin
Domain138 – 19861v-SNARE coiled-coil homology

Amino acid modifications

Modified residue1951Cysteine methyl ester Probable
Lipidation1941S-palmitoyl cysteine Ref.6 Ref.9
Lipidation1951S-farnesyl cysteine Ref.9

Experimental info

Mutagenesis421F → E: Increases palmitoylation. Targeted to Golgi membranes. Targeted to Golgi and cytosol; when associated with S-194. Targeted to cytosol; when associated with S-195. Ref.6 Ref.9
Mutagenesis1941C → S: Decreases palmitoylation by 55%. Prevents palmitoylation; when associated with S-195. Targeted to Golgi and cytosol; when associated with E-42. Ref.6 Ref.9
Mutagenesis1951C → S: Prevents farnesylation. Targeted to cytosol; when associated with E-42. Decreases palmitoylation by 13%. Prevents palmitoylation; when associated with S-194. Ref.6 Ref.9
Sequence conflict631D → N in CAG46805. Ref.3
Sequence conflict1381P → L in BAD97208. Ref.4
Sequence conflict1981M → I in CAG33270. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O15498 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 2AB0C12832D1B943

FASTA19822,418
        10         20         30         40         50         60 
MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER SSKGTRASVK 

        70         80         90        100        110        120 
EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE FSKQVDRIDW PVGSPATIHY 

       130        140        150        160        170        180 
PALDGHLSRY QNPREADPMT KVQAELDETK IILHNTMESL LERGEKLDDL VSKSEVLGTQ 

       190 
SKAFYKTARK QNSCCAIM 

« Hide

References

« Hide 'large scale' references
[1]"Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport."
McNew J.A., Soegaard M., Lampen N.M., Machida S., Ye R.R., Lacomis L., Tempst P., Rothman J.E., Soellner T.H.
J. Biol. Chem. 272:17776-17783(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Pancreas.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hepatoma.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymph.
[6]"The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues."
Veit M.
Biochem. J. 384:233-237(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, PALMITOYLATION AT CYS-194.
[7]"Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack."
Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M., Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.
Mol. Biol. Cell 15:1506-1518(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network."
Tai G., Lu L., Wang T.L., Tang B.L., Goud B., Johannes L., Hong W.
Mol. Biol. Cell 15:4011-4022(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation."
Fukasawa M., Varlamov O., Eng W.S., Soellner T.H., Rothman J.E.
Proc. Natl. Acad. Sci. U.S.A. 101:4815-4820(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, ISOPRENYLATION AT CYS-195, PALMITOYLATION.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U95735 mRNA. Translation: AAB81131.1.
BT007078 mRNA. Translation: AAP35741.1.
CR456989 mRNA. Translation: CAG33270.1.
CR542008 mRNA. Translation: CAG46805.1.
AK223488 mRNA. Translation: BAD97208.1.
BC007319 mRNA. Translation: AAH07319.1.
CCDSCCDS5482.1.
RefSeqNP_006546.1. NM_006555.3.
UniGeneHs.520794.

3D structure databases

ProteinModelPortalO15498.
SMRO15498. Positions 1-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115895. 10 interactions.
IntActO15498. 1 interaction.
MINTMINT-2999058.
STRING9606.ENSP00000223369.

PTM databases

PhosphoSiteO15498.

Proteomic databases

MaxQBO15498.
PaxDbO15498.
PRIDEO15498.

Protocols and materials databases

DNASU10652.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000223369; ENSP00000223369; ENSG00000106636.
GeneID10652.
KEGGhsa:10652.
UCSCuc003tkm.3. human.

Organism-specific databases

CTD10652.
GeneCardsGC07P044207.
HGNCHGNC:16959. YKT6.
HPAHPA030817.
HPA030818.
MIM606209. gene.
neXtProtNX_O15498.
PharmGKBPA145007308.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5143.
HOGENOMHOG000189970.
HOVERGENHBG088939.
InParanoidO15498.
KOK08516.
OMAKQNSCCE.
PhylomeDBO15498.
TreeFamTF105606.

Gene expression databases

ArrayExpressO15498.
BgeeO15498.
CleanExHS_YKT6.
GenevestigatorO15498.

Family and domain databases

Gene3D3.30.450.50. 1 hit.
InterProIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
SUPFAMSSF64356. SSF64356. 1 hit.
PROSITEPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiYKT6.
GenomeRNAi10652.
NextBio40495.
PROO15498.
SOURCESearch...

Entry information

Entry nameYKT6_HUMAN
AccessionPrimary (citable) accession number: O15498
Secondary accession number(s): Q53F01, Q6FGU9, Q6IB15
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM