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Protein

Synaptobrevin homolog YKT6

Gene

YKT6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.2 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • protein-cysteine S-palmitoyltransferase activity Source: HGNC
  • SNAP receptor activity Source: HGNC
  • SNARE binding Source: GO_Central

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: HGNC
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to Golgi Source: HGNC
  • vesicle docking involved in exocytosis Source: HGNC
  • vesicle fusion Source: GO_Central
  • vesicle targeting Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106636-MONOMER.
ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptobrevin homolog YKT6 (EC:2.3.1.-)
Gene namesi
Name:YKT6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:16959. YKT6.

Subcellular locationi

GO - Cellular componenti

  • apical dendrite Source: Ensembl
  • basilar dendrite Source: Ensembl
  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: BHF-UCL
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: HGNC
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: Reactome
  • endosome Source: HGNC
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: HGNC
  • Golgi membrane Source: Reactome
  • integral component of plasma membrane Source: HGNC
  • mitochondrion Source: HPA
  • neuronal cell body Source: Ensembl
  • SNARE complex Source: HGNC
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42F → E: Increases palmitoylation. Targeted to Golgi membranes. Targeted to Golgi and cytosol; when associated with S-194. Targeted to cytosol; when associated with S-195. 2 Publications1
Mutagenesisi194C → S: Decreases palmitoylation by 55%. Prevents palmitoylation; when associated with S-195. Targeted to Golgi and cytosol; when associated with E-42. 2 Publications1
Mutagenesisi195C → S: Prevents farnesylation. Targeted to cytosol; when associated with E-42. Decreases palmitoylation by 13%. Prevents palmitoylation; when associated with S-194. 2 Publications1

Organism-specific databases

DisGeNETi10652.
OpenTargetsiENSG00000106636.
PharmGKBiPA145007308.

Polymorphism and mutation databases

BioMutaiYKT6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002807091 – 195Synaptobrevin homolog YKT6Add BLAST195
PropeptideiPRO_0000396661196 – 198Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159PhosphoserineCombined sources1
Lipidationi194S-palmitoyl cysteine1 Publication1
Modified residuei195Cysteine methyl esterCurated1
Lipidationi195S-farnesyl cysteine1 Publication1

Post-translational modificationi

Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting.2 Publications
Farnesylation is required for Golgi targeting.

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein, Prenylation

Proteomic databases

EPDiO15498.
MaxQBiO15498.
PaxDbiO15498.
PeptideAtlasiO15498.
PRIDEiO15498.
TopDownProteomicsiO15498-1. [O15498-1]

PTM databases

iPTMnetiO15498.
PhosphoSitePlusiO15498.
SwissPalmiO15498.

Expressioni

Gene expression databases

BgeeiENSG00000106636.
CleanExiHS_YKT6.
ExpressionAtlasiO15498. baseline and differential.
GenevisibleiO15498. HS.

Organism-specific databases

HPAiHPA030817.
HPA030818.

Interactioni

Subunit structurei

Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5.By similarity

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • SNAP receptor activity Source: HGNC
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi115895. 48 interactors.
IntActiO15498. 5 interactors.
MINTiMINT-2999058.
STRINGi9606.ENSP00000223369.

Structurei

3D structure databases

ProteinModelPortaliO15498.
SMRiO15498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 126LonginPROSITE-ProRule annotationAdd BLAST119
Domaini138 – 198v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Domaini

The longin domain regulates palmitoylation and membrane targeting.

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 longin domain.PROSITE-ProRule annotation
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0861. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00390000015164.
HOGENOMiHOG000189970.
HOVERGENiHBG088939.
InParanoidiO15498.
KOiK08516.
OMAiGNQSKAF.
OrthoDBiEOG091G0MPE.
PhylomeDBiO15498.
TreeFamiTF105606.

Family and domain databases

Gene3Di3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O15498-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER
60 70 80 90 100
SSKGTRASVK EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE
110 120 130 140 150
FSKQVDRIDW PVGSPATIHY PALDGHLSRY QNPREADPMT KVQAELDETK
160 170 180 190
IILHNTMESL LERGEKLDDL VSKSEVLGTQ SKAFYKTARK QNSCCAIM
Length:198
Mass (Da):22,418
Last modified:January 1, 1998 - v1
Checksum:i2AB0C12832D1B943
GO
Isoform 2 (identifier: O15498-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-187: Missing.

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):18,580
Checksum:i660854BBA5AC6C27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63D → N in CAG46805 (Ref. 4) Curated1
Sequence conflicti138P → L in BAD97208 (Ref. 5) Curated1
Sequence conflicti198M → I in CAG33270 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056071154 – 187Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95735 mRNA. Translation: AAB81131.1.
BT007078 mRNA. Translation: AAP35741.1.
AK299158 mRNA. Translation: BAG61206.1.
CR456989 mRNA. Translation: CAG33270.1.
CR542008 mRNA. Translation: CAG46805.1.
AK223488 mRNA. Translation: BAD97208.1.
AC006454 Genomic DNA. No translation available.
BC007319 mRNA. Translation: AAH07319.1.
CCDSiCCDS5482.1. [O15498-1]
RefSeqiNP_006546.1. NM_006555.3. [O15498-1]
XP_016867171.1. XM_017011682.1. [O15498-2]
UniGeneiHs.520794.

Genome annotation databases

EnsembliENST00000223369; ENSP00000223369; ENSG00000106636. [O15498-1]
ENST00000496112; ENSP00000420805; ENSG00000106636. [O15498-2]
GeneIDi10652.
KEGGihsa:10652.
UCSCiuc003tkm.4. human. [O15498-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95735 mRNA. Translation: AAB81131.1.
BT007078 mRNA. Translation: AAP35741.1.
AK299158 mRNA. Translation: BAG61206.1.
CR456989 mRNA. Translation: CAG33270.1.
CR542008 mRNA. Translation: CAG46805.1.
AK223488 mRNA. Translation: BAD97208.1.
AC006454 Genomic DNA. No translation available.
BC007319 mRNA. Translation: AAH07319.1.
CCDSiCCDS5482.1. [O15498-1]
RefSeqiNP_006546.1. NM_006555.3. [O15498-1]
XP_016867171.1. XM_017011682.1. [O15498-2]
UniGeneiHs.520794.

3D structure databases

ProteinModelPortaliO15498.
SMRiO15498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115895. 48 interactors.
IntActiO15498. 5 interactors.
MINTiMINT-2999058.
STRINGi9606.ENSP00000223369.

PTM databases

iPTMnetiO15498.
PhosphoSitePlusiO15498.
SwissPalmiO15498.

Polymorphism and mutation databases

BioMutaiYKT6.

Proteomic databases

EPDiO15498.
MaxQBiO15498.
PaxDbiO15498.
PeptideAtlasiO15498.
PRIDEiO15498.
TopDownProteomicsiO15498-1. [O15498-1]

Protocols and materials databases

DNASUi10652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223369; ENSP00000223369; ENSG00000106636. [O15498-1]
ENST00000496112; ENSP00000420805; ENSG00000106636. [O15498-2]
GeneIDi10652.
KEGGihsa:10652.
UCSCiuc003tkm.4. human. [O15498-1]

Organism-specific databases

CTDi10652.
DisGeNETi10652.
GeneCardsiYKT6.
HGNCiHGNC:16959. YKT6.
HPAiHPA030817.
HPA030818.
MIMi606209. gene.
neXtProtiNX_O15498.
OpenTargetsiENSG00000106636.
PharmGKBiPA145007308.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0861. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00390000015164.
HOGENOMiHOG000189970.
HOVERGENiHBG088939.
InParanoidiO15498.
KOiK08516.
OMAiGNQSKAF.
OrthoDBiEOG091G0MPE.
PhylomeDBiO15498.
TreeFamiTF105606.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000106636-MONOMER.
ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811438. Intra-Golgi traffic.

Miscellaneous databases

ChiTaRSiYKT6. human.
GeneWikiiYKT6.
GenomeRNAii10652.
PROiO15498.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106636.
CleanExiHS_YKT6.
ExpressionAtlasiO15498. baseline and differential.
GenevisibleiO15498. HS.

Family and domain databases

Gene3Di3.30.450.50. 1 hit.
InterProiIPR011012. Longin-like_dom.
IPR010908. Longin_dom.
IPR001388. Synaptobrevin.
[Graphical view]
PfamiPF13774. Longin. 1 hit.
PF00957. Synaptobrevin. 1 hit.
[Graphical view]
SMARTiSM01270. Longin. 1 hit.
[Graphical view]
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYKT6_HUMAN
AccessioniPrimary (citable) accession number: O15498
Secondary accession number(s): B4DR94
, Q53F01, Q6FGU9, Q6IB15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.