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O15498

- YKT6_HUMAN

UniProt

O15498 - YKT6_HUMAN

Protein

Synaptobrevin homolog YKT6

Gene

YKT6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.2 Publications

    GO - Molecular functioni

    1. protein-cysteine S-palmitoyltransferase activity Source: HGNC
    2. SNAP receptor activity Source: HGNC

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: HGNC
    2. metabolic process Source: GOC
    3. protein transport Source: UniProtKB-KW
    4. retrograde transport, endosome to Golgi Source: HGNC
    5. vesicle docking involved in exocytosis Source: HGNC
    6. vesicle targeting Source: HGNC

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptobrevin homolog YKT6 (EC:2.3.1.-)
    Gene namesi
    Name:YKT6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:16959. YKT6.

    Subcellular locationi

    Cytoplasmcytosol. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side
    Note: Probably cycles through vesicles between Golgi and endosomes.

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. cytosol Source: UniProtKB-SubCell
    4. endoplasmic reticulum Source: HGNC
    5. endosome Source: HGNC
    6. extracellular vesicular exosome Source: UniProt
    7. Golgi apparatus Source: HGNC
    8. Golgi membrane Source: UniProtKB-SubCell
    9. integral component of plasma membrane Source: HGNC
    10. mitochondrion Source: HPA
    11. SNARE complex Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi42 – 421F → E: Increases palmitoylation. Targeted to Golgi membranes. Targeted to Golgi and cytosol; when associated with S-194. Targeted to cytosol; when associated with S-195. 2 Publications
    Mutagenesisi194 – 1941C → S: Decreases palmitoylation by 55%. Prevents palmitoylation; when associated with S-195. Targeted to Golgi and cytosol; when associated with E-42. 2 Publications
    Mutagenesisi195 – 1951C → S: Prevents farnesylation. Targeted to cytosol; when associated with E-42. Decreases palmitoylation by 13%. Prevents palmitoylation; when associated with S-194. 2 Publications

    Organism-specific databases

    PharmGKBiPA145007308.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Synaptobrevin homolog YKT6PRO_0000280709Add
    BLAST
    Propeptidei196 – 1983Removed in mature formCuratedPRO_0000396661

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi194 – 1941S-palmitoyl cysteine2 Publications
    Modified residuei195 – 1951Cysteine methyl esterCurated
    Lipidationi195 – 1951S-farnesyl cysteine1 Publication

    Post-translational modificationi

    Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting.2 Publications
    Farnesylation is required for Golgi targeting.

    Keywords - PTMi

    Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    MaxQBiO15498.
    PaxDbiO15498.
    PRIDEiO15498.

    PTM databases

    PhosphoSiteiO15498.

    Expressioni

    Gene expression databases

    ArrayExpressiO15498.
    BgeeiO15498.
    CleanExiHS_YKT6.
    GenevestigatoriO15498.

    Organism-specific databases

    HPAiHPA030817.
    HPA030818.

    Interactioni

    Subunit structurei

    Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5.By similarity

    Protein-protein interaction databases

    BioGridi115895. 9 interactions.
    IntActiO15498. 1 interaction.
    MINTiMINT-2999058.
    STRINGi9606.ENSP00000223369.

    Structurei

    3D structure databases

    ProteinModelPortaliO15498.
    SMRiO15498. Positions 1-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 126119LonginPROSITE-ProRule annotationAdd
    BLAST
    Domaini138 – 19861v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The longin domain regulates palmitoylation and membrane targeting.

    Sequence similaritiesi

    Belongs to the synaptobrevin family.Curated
    Contains 1 longin domain.PROSITE-ProRule annotation
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5143.
    HOGENOMiHOG000189970.
    HOVERGENiHBG088939.
    InParanoidiO15498.
    KOiK08516.
    OMAiKQNSCCE.
    PhylomeDBiO15498.
    TreeFamiTF105606.

    Family and domain databases

    Gene3Di3.30.450.50. 1 hit.
    InterProiIPR011012. Longin-like_dom.
    IPR010908. Longin_dom.
    IPR001388. Synaptobrevin.
    [Graphical view]
    PfamiPF13774. Longin. 1 hit.
    PF00957. Synaptobrevin. 1 hit.
    [Graphical view]
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS50859. LONGIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O15498-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER    50
    SSKGTRASVK EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE 100
    FSKQVDRIDW PVGSPATIHY PALDGHLSRY QNPREADPMT KVQAELDETK 150
    IILHNTMESL LERGEKLDDL VSKSEVLGTQ SKAFYKTARK QNSCCAIM 198
    Length:198
    Mass (Da):22,418
    Last modified:January 1, 1998 - v1
    Checksum:i2AB0C12832D1B943
    GO
    Isoform 2 (identifier: O15498-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         154-187: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:164
    Mass (Da):18,580
    Checksum:i660854BBA5AC6C27
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631D → N in CAG46805. 1 PublicationCurated
    Sequence conflicti138 – 1381P → L in BAD97208. 1 PublicationCurated
    Sequence conflicti198 – 1981M → I in CAG33270. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei154 – 18734Missing in isoform 2. 1 PublicationVSP_056071Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95735 mRNA. Translation: AAB81131.1.
    BT007078 mRNA. Translation: AAP35741.1.
    AK299158 mRNA. Translation: BAG61206.1.
    CR456989 mRNA. Translation: CAG33270.1.
    CR542008 mRNA. Translation: CAG46805.1.
    AK223488 mRNA. Translation: BAD97208.1.
    AC006454 Genomic DNA. No translation available.
    BC007319 mRNA. Translation: AAH07319.1.
    CCDSiCCDS5482.1.
    RefSeqiNP_006546.1. NM_006555.3.
    UniGeneiHs.520794.

    Genome annotation databases

    EnsembliENST00000223369; ENSP00000223369; ENSG00000106636.
    ENST00000496112; ENSP00000420805; ENSG00000106636.
    GeneIDi10652.
    KEGGihsa:10652.
    UCSCiuc003tkm.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95735 mRNA. Translation: AAB81131.1 .
    BT007078 mRNA. Translation: AAP35741.1 .
    AK299158 mRNA. Translation: BAG61206.1 .
    CR456989 mRNA. Translation: CAG33270.1 .
    CR542008 mRNA. Translation: CAG46805.1 .
    AK223488 mRNA. Translation: BAD97208.1 .
    AC006454 Genomic DNA. No translation available.
    BC007319 mRNA. Translation: AAH07319.1 .
    CCDSi CCDS5482.1.
    RefSeqi NP_006546.1. NM_006555.3.
    UniGenei Hs.520794.

    3D structure databases

    ProteinModelPortali O15498.
    SMRi O15498. Positions 1-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115895. 9 interactions.
    IntActi O15498. 1 interaction.
    MINTi MINT-2999058.
    STRINGi 9606.ENSP00000223369.

    PTM databases

    PhosphoSitei O15498.

    Proteomic databases

    MaxQBi O15498.
    PaxDbi O15498.
    PRIDEi O15498.

    Protocols and materials databases

    DNASUi 10652.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000223369 ; ENSP00000223369 ; ENSG00000106636 .
    ENST00000496112 ; ENSP00000420805 ; ENSG00000106636 .
    GeneIDi 10652.
    KEGGi hsa:10652.
    UCSCi uc003tkm.3. human.

    Organism-specific databases

    CTDi 10652.
    GeneCardsi GC07P044207.
    HGNCi HGNC:16959. YKT6.
    HPAi HPA030817.
    HPA030818.
    MIMi 606209. gene.
    neXtProti NX_O15498.
    PharmGKBi PA145007308.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5143.
    HOGENOMi HOG000189970.
    HOVERGENi HBG088939.
    InParanoidi O15498.
    KOi K08516.
    OMAi KQNSCCE.
    PhylomeDBi O15498.
    TreeFami TF105606.

    Miscellaneous databases

    GeneWikii YKT6.
    GenomeRNAii 10652.
    NextBioi 40495.
    PROi O15498.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O15498.
    Bgeei O15498.
    CleanExi HS_YKT6.
    Genevestigatori O15498.

    Family and domain databases

    Gene3Di 3.30.450.50. 1 hit.
    InterProi IPR011012. Longin-like_dom.
    IPR010908. Longin_dom.
    IPR001388. Synaptobrevin.
    [Graphical view ]
    Pfami PF13774. Longin. 1 hit.
    PF00957. Synaptobrevin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS50859. LONGIN. 1 hit.
    PS50892. V_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport."
      McNew J.A., Soegaard M., Lampen N.M., Machida S., Ye R.R., Lacomis L., Tempst P., Rothman J.E., Soellner T.H.
      J. Biol. Chem. 272:17776-17783(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
      Tissue: Pancreas.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Hepatoma.
    6. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lymph.
    8. "The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues."
      Veit M.
      Biochem. J. 384:233-237(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, PALMITOYLATION AT CYS-194.
    9. "Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack."
      Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M., Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.
      Mol. Biol. Cell 15:1506-1518(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network."
      Tai G., Lu L., Wang T.L., Tang B.L., Goud B., Johannes L., Hong W.
      Mol. Biol. Cell 15:4011-4022(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation."
      Fukasawa M., Varlamov O., Eng W.S., Soellner T.H., Rothman J.E.
      Proc. Natl. Acad. Sci. U.S.A. 101:4815-4820(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, ISOPRENYLATION AT CYS-195, PALMITOYLATION.
    12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYKT6_HUMAN
    AccessioniPrimary (citable) accession number: O15498
    Secondary accession number(s): B4DR94
    , Q53F01, Q6FGU9, Q6IB15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3