Reviewed, UniProtKB/Swiss-Prot O15498 (YKT6_HUMAN)

Names and origin

Protein names

Recommended name:
    Synaptobrevin homolog YKT6
    EC=2.3.1.-

Gene names

Name:

YKT6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity. Ref.1 Ref.7
Subunit structure	Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5 By similarity.
Subcellular location	Cytoplasm › cytosol. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Note: Probably cycles through vesicles between Golgi and endosomes. Ref.7 Ref.8
Domain	The longin domain regulates palmitoylation and membrane targeting.
Post-translational modification	Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting. Ref.8 Ref.6 Farnesylation is required for Golgi targeting.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Coiled coil
Molecular function	Transferase
PTM	Lipoprotein Palmitate Prenylation
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Ref.1 Inferred from direct assay. Source: HGNC protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Ref.7 Inferred from direct assay. Source: HGNC vesicle docking during exocytosis Ref.1 Inferred from direct assay. Source: HGNC vesicle targeting Ref.1 Inferred from direct assay. Source: HGNC
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Ref.7 Traceable author statement. Source: HGNC cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Ref.1 Inferred from direct assay. Source: HGNC endosome Ref.7 Inferred from direct assay. Source: HGNC integral to plasma membrane Ref.1 Inferred from direct assay. Source: HGNC
Molecular function	SNAP receptor activity Ref.7 Inferred from direct assay. Source: HGNC protein-cysteine S-palmitoleyltransferase activity Ref.6 Inferred from direct assay. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Synaptobrevin homolog YKT6		PRO_0000280709
Regions
	Domain	8 – 126	119	Longin
	Domain	138 – 198	61	v-SNARE coiled-coil homology
Amino acid modifications
	Lipidation	194	1	S-palmitoyl cysteine Ref.8 Ref.6
	Lipidation	195	1	S-farnesyl cysteine
Experimental info
	Mutagenesis	42	1	F → E: Increases palmitoylation. Targeted to Golgi membranes. Targeted to Golgi and cytosol; when associated with S-194. Targeted to cytosol; when associated with S-195. Ref.8 Ref.6
	Mutagenesis	194	1	C → S: Decreases palmitoylation by 55%. Prevents palmitoylation; when associated with S-195. Targeted to Golgi and cytosol; when associated with E-42. Ref.8 Ref.6
	Mutagenesis	195	1	C → S: Prevents farnesylation. Targeted to cytosol; when associated with E-42. Decreases palmitoylation by 13%. Prevents palmitoylation; when associated with S-194. Ref.8 Ref.6
	Sequence conflict	63	1	D → N in CAG46805. Ref.3
	Sequence conflict	138	1	P → L in BAD97208. Ref.4
	Sequence conflict	198	1	M → I in CAG33270. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O15498-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2AB0C12832D1B943	FASTA	198	22,418
	10 20 30 40 50 60 MKLYSLSVLY KGEAKVVLLK AAYDVSSFSF FQRSSVQEFM TFTSQLIVER SSKGTRASVK 70 80 90 100 110 120 EQDYLCHVYV RNDSLAGVVI ADNEYPSRVA FTLLEKVLDE FSKQVDRIDW PVGSPATIHY 130 140 150 160 170 180 PALDGHLSRY QNPREADPMT KVQAELDETK IILHNTMESL LERGEKLDDL VSKSEVLGTQ 190 SKAFYKTARK QNSCCAIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport." McNew J.A., Soegaard M., Lampen N.M., Machida S., Ye R.R., Lacomis L., Tempst P., Rothman J.E., Soellner T.H. J. Biol. Chem. 272:17776-17783(1997) [PubMed: 9211930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Pancreas.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hepatoma.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[6]	"The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues." Veit M. Biochem. J. 384:233-237(2004) [PubMed: 15479160] [Abstract] Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, PALMITOYLATION AT CYS-194.
[7]	"Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network." Tai G., Lu L., Wang T.L., Tang B.L., Goud B., Johannes L., Hong W. Mol. Biol. Cell 15:4011-4022(2004) [PubMed: 15215310] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation." Fukasawa M., Varlamov O., Eng W.S., Soellner T.H., Rothman J.E. Proc. Natl. Acad. Sci. U.S.A. 101:4815-4820(2004) [PubMed: 15044687] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-42; CYS-194 AND CYS-195, ISOPRENYLATION AT CYS-195, PALMITOYLATION.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U95735 mRNA. Translation: AAB81131.1. BT007078 mRNA. Translation: AAP35741.1. CR456989 mRNA. Translation: CAG33270.1. CR542008 mRNA. Translation: CAG46805.1. AK223488 mRNA. Translation: BAD97208.1. BC007319 mRNA. Translation: AAH07319.1.
IPI	IPI00008569.
RefSeq	NP_006546.1.
UniGene	Hs.520794
3D structure databases
HSSP	HSSP built from PDB template 1IOU based on UniProtKB P36015.
ModBase	Search...
Proteomic databases
PRIDE	O15498.
Genome annotation databases
Ensembl	ENSG00000106636. Homo sapiens. [Contig view]
GeneID	10652.
KEGG	hsa:10652.
Organism-specific databases
GeneCards	GC07P044207.
HGNC	HGNC:16959. YKT6.
MIM	606209. gene.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O15498.
OMA	O15498. KQNSCCE.
Gene expression databases
ArrayExpress	O15498.
Bgee	O15498.
CleanEx	HS_YKT6.
Family and domain databases
InterPro	IPR010908. Longin. IPR001388. Synaptobrevin. [Graphical view]
Gene3D	G3DSA:3.30.450.50. Longin. 1 hit.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
ProDom	PD001229. Synaptobrevin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50859. LONGIN. 1 hit. PS00417. SYNAPTOBREVIN. False negative. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40495.
SOURCE	Search...

Entry information

Entry name

YKT6_HUMAN

Accession

Primary (citable) accession number: O15498
Secondary accession number(s): Q53F01, Q6FGU9, Q6IB15

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)