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Protein

Regulator of G-protein signaling 16

Gene

RGS16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11602604, PubMed:18434541). Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha. May regulate extra and intracellular mitogenic signals (By similarity).By similarity2 Publications

GO - Molecular functioni

  • calmodulin binding Source: ProtInc
  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
  • visual perception Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143333-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.
SIGNORiO15492.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 16
Short name:
RGS16
Alternative name(s):
A28-RGS14P
Retinal-specific RGS
Short name:
RGS-r
Short name:
hRGS-r
Retinally abundant regulator of G-protein signaling
Gene namesi
Name:RGS16
Synonyms:RGSR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9997. RGS16.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cytoplasm Source: CACAO
  • intrinsic component of membrane Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168Y → F: 30% decrease in GAP activity. 1 Publication1
Mutagenesisi177Y → F: No effect on GAP activity. 1 Publication1

Organism-specific databases

DisGeNETi6004.
OpenTargetsiENSG00000143333.
PharmGKBiPA34367.

Polymorphism and mutation databases

BioMutaiRGS16.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002042211 – 202Regulator of G-protein signaling 16Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2S-palmitoyl cysteineBy similarity1
Lipidationi12S-palmitoyl cysteineBy similarity1
Modified residuei168Phosphotyrosine; by EGFR1 Publication1
Modified residuei177Phosphotyrosine1 Publication1

Post-translational modificationi

Palmitoylated on Cys-2 and/or Cys-12.By similarity
Phosphorylated. Phosphorylation at Tyr-168 by EGFR enhances GTPase accelerating (GAP) activity toward GNAI1.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO15492.
PeptideAtlasiO15492.
PRIDEiO15492.

PTM databases

iPTMnetiO15492.
PhosphoSitePlusiO15492.
SwissPalmiO15492.

Expressioni

Tissue specificityi

Abundantly expressed in retina with lower levels of expression in most other tissues.

Gene expression databases

BgeeiENSG00000143333.
CleanExiHS_RGS16.
GenevisibleiO15492. HS.

Organism-specific databases

HPAiHPA053250.
HPA055824.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAQ (PubMed:18434541). Interacts with GNAI2, GNAI3 and GNAO1 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calmodulin binding Source: ProtInc

Protein-protein interaction databases

BioGridi111936. 14 interactors.
DIPiDIP-59094N.
IntActiO15492. 2 interactors.
STRINGi9606.ENSP00000356529.

Chemistry databases

BindingDBiO15492.

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 62Combined sources10
Helixi66 – 70Combined sources5
Helixi73 – 85Combined sources13
Helixi89 – 101Combined sources13
Helixi107 – 121Combined sources15
Helixi134 – 143Combined sources10
Helixi144 – 146Combined sources3
Turni149 – 152Combined sources4
Helixi153 – 165Combined sources13
Helixi167 – 173Combined sources7
Helixi175 – 186Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BT2X-ray1.90A/B/C/D/E53-190[»]
2IK8X-ray2.71B/D53-190[»]
ProteinModelPortaliO15492.
SMRiO15492.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15492.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 181RGSPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO15492.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiO15492.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O15492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRTLAAFPT TCLERAKEFK TRLGIFLHKS ELGCDTGSTG KFEWGSKHSK
60 70 80 90 100
ENRNFSEDVL GWRESFDLLL SSKNGVAAFH AFLKTEFSEE NLEFWLACEE
110 120 130 140 150
FKKIRSATKL ASRAHQIFEE FICSEAPKEV NIDHETHELT RMNLQTATAT
160 170 180 190 200
CFDAAQGKTR TLMEKDSYPR FLKSPAYRDL AAQASAASAT LSSCSLDEPS

HT
Length:202
Mass (Da):22,749
Last modified:January 11, 2011 - v2
Checksum:i19B384E935F3E5D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42F → S in AAC16912 (PubMed:9223279).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046528137H → R.7 PublicationsCorresponds to variant rs1144566dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70426 mRNA. Translation: AAC16912.1.
U94829 mRNA. Translation: AAC52040.1.
AF009356 Genomic DNA. Translation: AAC39642.1.
AF493937 mRNA. Translation: AAM12651.1.
BT006638 mRNA. Translation: AAP35284.1.
AK311880 mRNA. Translation: BAG34821.1.
AL353778 Genomic DNA. Translation: CAH72419.1.
CH471067 Genomic DNA. Translation: EAW91129.1.
BC006243 mRNA. Translation: AAH06243.1.
CCDSiCCDS1348.1.
RefSeqiNP_002919.3. NM_002928.3.
UniGeneiHs.413297.

Genome annotation databases

EnsembliENST00000367558; ENSP00000356529; ENSG00000143333.
GeneIDi6004.
KEGGihsa:6004.
UCSCiuc001gpl.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70426 mRNA. Translation: AAC16912.1.
U94829 mRNA. Translation: AAC52040.1.
AF009356 Genomic DNA. Translation: AAC39642.1.
AF493937 mRNA. Translation: AAM12651.1.
BT006638 mRNA. Translation: AAP35284.1.
AK311880 mRNA. Translation: BAG34821.1.
AL353778 Genomic DNA. Translation: CAH72419.1.
CH471067 Genomic DNA. Translation: EAW91129.1.
BC006243 mRNA. Translation: AAH06243.1.
CCDSiCCDS1348.1.
RefSeqiNP_002919.3. NM_002928.3.
UniGeneiHs.413297.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BT2X-ray1.90A/B/C/D/E53-190[»]
2IK8X-ray2.71B/D53-190[»]
ProteinModelPortaliO15492.
SMRiO15492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111936. 14 interactors.
DIPiDIP-59094N.
IntActiO15492. 2 interactors.
STRINGi9606.ENSP00000356529.

Chemistry databases

BindingDBiO15492.

PTM databases

iPTMnetiO15492.
PhosphoSitePlusiO15492.
SwissPalmiO15492.

Polymorphism and mutation databases

BioMutaiRGS16.

Proteomic databases

PaxDbiO15492.
PeptideAtlasiO15492.
PRIDEiO15492.

Protocols and materials databases

DNASUi6004.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367558; ENSP00000356529; ENSG00000143333.
GeneIDi6004.
KEGGihsa:6004.
UCSCiuc001gpl.5. human.

Organism-specific databases

CTDi6004.
DisGeNETi6004.
GeneCardsiRGS16.
H-InvDBHIX0001402.
HGNCiHGNC:9997. RGS16.
HPAiHPA053250.
HPA055824.
MIMi602514. gene.
neXtProtiNX_O15492.
OpenTargetsiENSG00000143333.
PharmGKBiPA34367.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO15492.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiO15492.
TreeFamiTF315837.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143333-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.
SIGNORiO15492.

Miscellaneous databases

EvolutionaryTraceiO15492.
GeneWikiiRGS16.
GenomeRNAii6004.
PROiO15492.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143333.
CleanExiHS_RGS16.
GenevisibleiO15492. HS.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS16_HUMAN
AccessioniPrimary (citable) accession number: O15492
Secondary accession number(s): B2R4M4, Q5VYN9, Q99701
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.