O15484 (CAN5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-5
EC=3.4.22.-

Alternative name(s):
Calpain htra-3
New calpain 3
Short name=nCL-3

Gene names

Name:	CAPN5
Synonyms:	NCL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Broad endopeptidase specificity.
Tissue specificity	Expressed in many tissues.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 C2 domain. Contains 1 calpain catalytic domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Traceable author statement Ref.3. Source: ProtInc
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium-dependent cysteine-type endopeptidase activity Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Calpain-5		PRO_0000207713
Regions
	Domain	26 – 343	318	Calpain catalytic
	Domain	518 – 619	102	C2
	Region	344 – 496	153	Domain III
Sites
	Active site	81	1	By similarity
	Active site	252	1	By similarity
	Active site	284	1	By similarity
Experimental info
	Sequence conflict	18	1	R → Q in AAC51869. Ref.1
	Sequence conflict	51	1	W → R in CAA71584. Ref.3
	Sequence conflict	112 – 115	4	EKPN → RKAQ in AAC51869. Ref.1
	Sequence conflict	128 – 131	4	FGEW → LGM in AAC51869. Ref.1
	Sequence conflict	138	1	D → E in AAC51869. Ref.1
	Sequence conflict	502	1	E → K in AAC51869. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15484 [UniParc]. Last modified February 12, 2003. Version 2. Checksum: 7A3A9A1A920410BC	FASTA	640	73,169
	10 20 30 40 50 60 MFSCVKPYED QNYSALRRDC RRRKVLFEDP LFPATDDSLY YKGTPGPAVR WKRPKGICED 70 80 90 100 110 120 PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWD PEKPNAYAGI 130 140 150 160 170 180 FHFHFWRFGE WVDVVIDDRL PTVNNQLIYC HSNSRNEFWC ALVEKAYAKL AGCYQALDGG 190 200 210 220 230 240 NTADALVDFT GGVSEPIDLT EGDFANDETK RNQLFERMLK VHSRGGLISA SIKAVTAADM 250 260 270 280 290 300 EARLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WNGPWSDTSE 310 320 330 340 350 360 EWQKVSKSER EKMGVTVQDD GEFWMTFEDV CRYFTDIIKC RVINTSHLSI HKTWEEARLH 370 380 390 400 410 420 GAWTLHEDPR QNRGGGCINH KDTFFQNPQY IFEVKKPEDE VLICIQQRPK RSTRREGKGE 430 440 450 460 470 480 NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TDQPEGRYVI IPTTFEPGHT 490 500 510 520 530 540 GEFLLRVFTD VPSNCRELRL DEPPHTCWSS LCGYPQLVTQ VHVLGAAGLK DSPTGANSYV 550 560 570 580 590 600 IIKCEGDKVR SAVQKGTSTP EYNVKGIFYR KKLSQPITVQ VWNHRVLKDE FLGQVHLKAD 610 620 630 640 PDNLQALHTL HLRDRNSRQP SNLPGTVAVH ILSSTSLMAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel, tissue-specific calpain htra-3; a human homologue of the Caenorhabditis elegans sex determination gene." Mugita N., Kimura Y., Ogawa M., Saya H., Nakao M. Biochem. Biophys. Res. Commun. 239:845-850(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[3]	"A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution." Dear T.N., Matena K., Vingron M., Boehm T. Genomics 45:175-184(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-633. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U94346 mRNA. Translation: AAC51869.1. BC018123 mRNA. Translation: AAH18123.1. Y10552 mRNA. Translation: CAA71584.1.
IPI	IPI00742984.
PIR	JC5772.
RefSeq	NP_004046.2. NM_004055.4.
UniGene	Hs.248153.
3D structure databases
ProteinModelPortal	O15484.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15484. 1 interaction.
STRING	9606.ENSP00000278559.
Protein family/group databases
MEROPS	C02.011.
PTM databases
PhosphoSite	O15484.
Proteomic databases
PaxDb	O15484.
PRIDE	O15484.
Protocols and materials databases
DNASU	726.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000278559; ENSP00000278559; ENSG00000149260. ENST00000529629; ENSP00000432332; ENSG00000149260. ENST00000571230; ENSP00000461719; ENSG00000262875. ENST00000576267; ENSP00000458516; ENSG00000262875.
GeneID	726.
KEGG	hsa:726.
UCSC	uc001oxx.3. human.
Organism-specific databases
CTD	726.
GeneCards	GC11P076777.
HGNC	HGNC:1482. CAPN5.
HPA	CAB033237.
MIM	602537. gene.
neXtProt	NX_O15484.
PharmGKB	PA26062.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG258770.
HOGENOM	HOG000232036.
HOVERGEN	HBG001095.
InParanoid	O15484.
KO	K08574.
OrthoDB	EOG4DV5KQ.
PhylomeDB	O15484.
Gene expression databases
ArrayExpress	O15484.
Bgee	O15484.
CleanEx	HS_CAPN5.
Genevestigator	O15484.
GermOnline	ENSG00000149260. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF00168. C2. 1 hit. PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00239. C2. 1 hit. SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50004. C2. False negative. PS50203. CALPAIN_CAT. 1 hit. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	726.
NextBio	2956.
SOURCE	Search...

Entry information

Entry name

CAN5_HUMAN

Accession

Primary (citable) accession number: O15484
Secondary accession number(s): O00263

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	February 12, 2003
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.